Microwave assisted extraction of antiplasmodial and antioxidant limonoids from Trichilia roka (chiov)

Trichilia roka (Chiov) is a medicinal plant from Meliaceae family. It is recognized in traditional medicine for its innumerable therapeutic properties. Limonoids, the main constituents of the root bark of this plant, is known for its antioxidant and antiplasmodial activities. To obtain an improved yield of these bioactive compounds from T. roka and reduce extraction time, solvent and energy required, it is of utmost importance to adopt innovative approaches such as microwave-assisted extraction. Microwave was attempted, as compared with the conventional mechanical agitation method to extract bioactive limonoids and quantify them through colorimetric quantification method using 4(dimethyl amino) benzaldehyde (DMAB). The antiplasmodial activity was evaluated against the intraerythrocytic stages of cultured Plasmodium falciparum using a phenotypic approach, and the antioxidant property was evaluated in vitro using DPPH radical-scavenging and β-carotene-linoleate model systems respectively. Three limonoids and were isolated from Trichilia roka (Chiov) root bark labelled RA, RO and RY. The microwave extraction yields were 115.895 mgRUBE/gDW for limonoids. The isolated compounds exhibited good antioxidant activities than crude extracts with IC50 values of 2.59 10-3 , 2.26 10-3 and 1.79 10-3 mg/mL respectively compared to crude extract IC50 values of 2. 10-2 mg/mL. The in vivo antiplasmodial test of the hydromethanolic microwave extracts showed during the five treatment days the decreasing of the parasitaemia for doses 100 mg/kg, 200 mg/kg and 500 mg/kg with inhibition percentages of 82.75, 84.84 and 87.8 respectively

Download Full-text

Influence of microwave assisted extraction on antioxidant and antiplasmodial activities of Trichilia roka extracts

The Journal of Phytopharmacology ◽

10.31254/phyto.2015.4502 ◽

2015 ◽

Vol 4 (5) ◽

pp. 248-252

Author(s):

Olga Nana ◽

◽

Jean Momeni ◽

Fabrice Fekam Boyom ◽

Martin Benoît Ngassoum ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Model Systems ◽

Antiplasmodial Activity ◽

Root Bark ◽

Microwave Assisted Extraction ◽

Mechanical Agitation ◽

Microwave Assisted ◽

Assisted Extraction ◽

Β Carotene

Antiplasmodial and antioxidant activities of extracts derived from Trichilia roka (Chiov) (Meliaceae) root bark were determined respectively in vitro and using two methods as well as 1,1-diphenyl-2-picrylhydrazyl radicalscavenging and β-carotene-linoleate model systems. The microwave assisted extraction extract was more effective concern antioxydant activity than the antiplasmodial activity compare to conventional mechanical agitation extraction method. Extracts obtained by microwave assisted extraction showed a high total polyphenol content of 126766 μg Equivalent of Gallic acid /g Dry Mater and a total flavonoid content of 789.22 μg Equivalent of Quercetin/g dry mater but a low antiplasmodial activity with and inhibition concentration of 48.386 μg /mL and 23.983 μg/mL for mechanical agitation extract. The evaluation of the antioxidant properties of the two extracts showed that those obtained by microwave assisted extraction shown in the 1,1-diphenyl-2-picrylhydrazyl assay and in the β-carotene bleaching test, the highest antioxidant activity respectively, with an antioxidant activity of 82.12% and with a radical-scavenging activity with inhibition percentage of 88.78%..

Download Full-text

Microwave-assisted extraction of flavonoids from Phyllostachys heterocycla leaves: Optimization, mechanism, and antioxidant activity in vitro

BioResources ◽

10.15376/biores.16.4.8060-8081 ◽

2021 ◽

Vol 16 (4) ◽

pp. 8060-8081

Author(s):

Jie Xu ◽

Jianjun Wu ◽

Juan Qi ◽

Juan Li ◽

Yongju Liu ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Soxhlet Extraction ◽

Extraction Yield ◽

Microwave Assisted Extraction ◽

Extraction Technology ◽

Microwave Assisted ◽

Assisted Extraction ◽

Phyllostachys Heterocycla

Flavonoids were extracted from Phyllostachys heterocycla leaves by adopting microwave-assisted extraction technology. Based on the single factor experiment and Plackett-Burman design results, the extraction process of flavonoids was further optimized using the response surface methodology. The optimum conditions were as follows: an ethanol concentration of 78.1%, an extraction time of 24.9 min, and a microwave power of 559 W. Under these conditions, the extraction yield of flavonoids was 4.67%, which was in close proximity to the predicted value (4.70%) and higher than the extraction yield from traditional Soxhlet extraction (3.35%). Moreover, the possible extraction mechanisms of these two extraction methods were further derived to explain why the microwave-assisted extraction of flavonoids was more efficient compared with traditional Soxhlet extraction. Ultimately, the antioxidant activities in vitro of flavonoids from Phyllostachys heterocycla leaves were evaluated via DPPH and ABTS radical scavenging assay. The flavonoids from Phyllostachys heterocycla leaves exhibited excellent antioxidant activities in vitro and Phyllostachys heterocycla leaves could be a new natural source for developing antioxidants. Overall, the findings of this research could provide a theoretical reference for the further comprehensive development and utilization of bamboo resources.

Download Full-text

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Foods ◽

10.3390/foods9040456 ◽

2020 ◽

Vol 9 (4) ◽

pp. 456 ◽

Cited By ~ 1

Author(s):

Ding-Tao Wu ◽

Wen Liu ◽

Mei-Lin Xian ◽

Gang Du ◽

Xin Liu ◽

...

Keyword(s):

Physicochemical Properties ◽

Antioxidant Activities ◽

Extraction Methods ◽

Water Extraction ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Hovenia Dulcis

Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

Download Full-text

Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities

Archives of Biological Sciences ◽

10.2298/abs191216015w ◽

2020 ◽

Vol 72 (2) ◽

pp. 211-221

Author(s):

Xiang Wang ◽

Mi-Jun Peng ◽

Zhi-Hong Wang ◽

Qiu-Ling Yang ◽

Sheng Peng

Keyword(s):

Antibacterial Activities ◽

Eucommia Ulmoides ◽

Microwave Assisted Extraction ◽

Ultrasound Extraction ◽

Microwave Assisted ◽

Flavonoid Compounds ◽

Assisted Extraction ◽

Ic50 Values ◽

Burman Design

Ultrasound/microwave assisted extraction (UMAE) of flavonoid compounds from Eucommia ulmoides leaves was studied and the extraction conditions were optimized by the Plackett-Burman design (PBD) method combined with the Box-Behnken design (BBD). The antioxidant and antibacterial activities of the flavonoid extract were investigated. The results show that the optimal conditions were an ethanol concentration of 41%, microwave power of 178 W and an ultrasound extraction time of 26 min. Under these conditions, the yield of the flavonoid compounds was 2.454%?0.230%, which was higher than that after direct solvent extraction, ultrasound extraction and microwave extraction. The results of in vitro antioxidant assays showed that the flavonoid extract had scavenging capacity for DPPH, ABTS and hydroxyl radicals, with corresponding IC50 values of 30.76 mg/L, 21.09 mg/L, 248.4 mg/L, respectively. In addition, this extract exhibited strong antibacterial activity on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium.

Download Full-text

Alpha-Mangostin-Rich Extracts from Mangosteen Pericarp: Optimization of Green Extraction Protocol and Evaluation of Biological Activity

Molecules ◽

10.3390/molecules23081852 ◽

2018 ◽

Vol 23 (8) ◽

pp. 1852 ◽

Cited By ~ 9

Author(s):

Ali Ghasemzadeh ◽

Hawa Jaafar ◽

Ali Baghdadi ◽

Amin Tayebi-Meigooni

Keyword(s):

Antioxidant Activities ◽

Natural Antioxidants ◽

Extraction Process ◽

Total Phenolic ◽

Microwave Assisted Extraction ◽

Garcinia Mangostana ◽

Main Compound ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ic50 Values

Since α-mangostin in mangosteen fruits was reported to be the main compound able to provide natural antioxidants, the microwave-assisted extraction process to obtain high-quality α-mangostin from mangosteen pericarp (Garcinia mangostana L.) was optimized using a central composite design and response surface methodology. The parameters examined included extraction time, microwave power, and solvent percentage. The antioxidant and antimicrobial activity of optimized and non-optimized extracts was evaluated. Ethyl acetate as a green solvent exhibited the highest concentration of α-mangostin, followed by dichloromethane, ethanol, and water. The highest α-mangostin concentration in mangosteen pericarp of 121.01 mg/g dry matter (DM) was predicted at 3.16 min, 189.20 W, and 72.40% (v/v). The verification of experimental results under these optimized conditions showed that the α-mangostin value for the mangosteen pericarp was 120.68 mg/g DM. The predicted models were successfully developed to extract α-mangostin from the mangosteen pericarp. No significant differences were observed between the predicted and the experimental α-mangostin values, indicating that the developed models are accurate. The analysis of the extracts for secondary metabolites showed that the total phenolic content (TPC) and total flavonoid content (TFC) increased significantly in the optimized extracts (OE) compared to the non-optimized extracts (NOE). Additionally, trans-ferulic acid and catechin were abundant among the compounds identified. In addition, the optimized extract of mangosteen pericarp with its higher α-mangostin and secondary metabolite concentrations exhibited higher antioxidant activities with half maximal inhibitory concentration (IC50) values of 20.64 µg/mL compared to those of the NOE (28.50 µg/mL). The OE exhibited the highest antibacterial activity, particularly against Gram-positive bacteria. In this study, the microwave-assisted extraction process of α-mangostin from mangosteen pericarp was successfully optimized, indicating the accuracy of the models developed, which will be usable in a larger-scale extraction process.

Download Full-text