scholarly journals Effect of Carbafuran Pesticide on Mitotic Chromosomes of Mus musculus

2018 ◽  
Vol 6 (2) ◽  
pp. 169-173
Author(s):  
Manisha Kumari ◽  
Rohit Kumar Verma ◽  
Prabhat Kumar Roy
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Chromosomal Abnormalities ◽  
Bone Marrow Cells ◽  
Test Material ◽  
Mitotic Chromosomes ◽  
Swiss Albino Mice ◽  
Female Mice ◽  
Old Mice ◽  
Marrow Cells

The Genotoxic effect of the carbafuran pesticide was evaluated in the metaphasic chromosomes of bone marrow cells of female Swiss albino mice, Mus musculus . Six week old mice were put into different groups(six mice per group) and treatment were done according to experimental plan (CLD15 i.e. lower dose =30ppm or 0.03mg/ml and CHD30 i.e. higher dose =300ppm or 0.3mg/ml).The test material was administered orally for 15 days in CLD15 and for 30 days in CHD30 test groups of female mice. The result revealed that the frequency of chromosomal abnormalities i.e. structural and numerical changes were11.5% in control, 74.0%in CLD15 and 99.0%in CHD30, respectively in bone marrow cells of female mice.Int. J. Appl. Sci. Biotechnol. Vol 6(2): 169-173 

scholarly journals Genotoxic Effect of Silk Dyeing Wastes in Bone Marrow Cells of Mice, Mus musculus

CYTOLOGIA ◽  
2005 ◽  
Vol 70 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Om Prakash Chaurasia ◽  
Alok Kumar ◽  
Moushmi Kumari
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Bone Marrow Cells ◽  
Genotoxic Effect ◽  
Silk Dyeing ◽  
Marrow Cells

scholarly journals Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Sahdeo Prasad ◽  
Smita Srivastava ◽  
Madhulika Singh ◽  
Yogeshwer Shukla
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Positive Control ◽  
Vehicle Group ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Human Beings ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Marrow Cells

Glyphosate (N-(phosphonomethyl) glycine,C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and micronuclei (MN) in bone marrow cells of Swiss albino mice. A single dose of glyphosate was given intraperitoneally (i.p) to the animals at a concentration of 25 and 50 mg/kg b.wt. Animals of positive control group were injectedi.p. benzo(a)pyrene (100 mg/kg b.wt., once only), whereas, animals of control (vehicle) group were injectedi.p. dimethyl sulfoxide (0.2mL). Animals from all the groups were sacrificed at sampling times of 24, 48, and 72 hours and their bone marrow was analyzed for cytogenetic and chromosomal damage. Glyphosate treatment significantly increases CAs and MN induction at both treatments and time compared with the vehicle control (P<.05). The cytotoxic effects of glyphosate were also evident, as observed by significant decrease in mitotic index (MI). The present results indicate that glyphosate is clastogenic and cytotoxic to mouse bone marrow.

Environmental monitoring for genotoxicity: in vivo sister chromatid exchange in the house mouse (Mus musculus)

1985 ◽  
Vol 27 (3) ◽  
pp. 351-356 ◽  
Author(s):  
B. N. Nayak ◽  
M. L. Petras
Keyword(s):  
Bone Marrow ◽  
Sister Chromatid ◽  
Mus Musculus ◽  
Sister Chromatid Exchange ◽  
Bone Marrow Cells ◽  
Natural Populations ◽  
Wild Mice ◽  
Monitoring Procedure ◽  
Marrow Cells

Sister chromatid exchange (SCE) values were determined in bone marrow cells isolated from mouse (Mus musculus) femurs after injections of 5-bromo-2′-deoxyuridine (BrdU) and 5-fluorodeoxyuridine (FrdU). Male mice of C3H/J, C57BL/6J, and DBA/2 strains maintained in the laboratory gave mean SCE values of 3.42 ± 0.07, 3.62 ± 0.08, and 3.97 ± 0.13, respectively. Males obtained from natural populations of southwestern Ontario had a higher mean SCE value (6.02 ± 0.16), as did inbred males maintained in outdoor enclosures for at least 3 weeks (5.07 ± 0.22). Wild mice housed in the laboratory for 9 months or longer had SCE values similar to laboratory bred mice (3.46 ± 0.05). The SCE values in wild-caught mice were inversely proportional (r = −0.49) to the distance between the sites where these animals were collected and the nearest major industrial center. Based on these results, SCE analysis in mice is proposed as a possible first-line monitoring procedure for the detection of general changes in environmental genotoxicity.Key words: environmental genotoxicity, sister chromatid exchange, bone marrow cells, BrdU, FrdU, mouse.

scholarly journals G-banding analysis of complex aneuploidy in multiple myeloma bone marrow cells

Blood ◽  
1976 ◽  
Vol 47 (1) ◽  
pp. 69-77 ◽  
Author(s):  
P Philip ◽  
A Drivsholm
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Chromosomal Abnormalities ◽  
Bone Marrow Cells ◽  
Clonal Evolution ◽  
Banding Technique ◽  
Chromosome Loss ◽  
Banding Analysis ◽  
Myeloproliferative Diseases ◽  
Marrow Cells

Abstract Chromosome studies with the banding technique have been performed in a considerable number of cases of myeloproliferative diseases, but technical difficulties have so far prevented detailed studies of chromosomal abnormalities in multiple myeloma. The karyotypes of bone marrow cells from two patients with multiple myeloma have been analyzed by a trypsin-Giemsa banding technique. Evidence is given for clonal evolution which in one patient has probably occurred by cell fusion and subsequent chromosome loss. Eight different marker chromosomes are characterized. Nonrandom chromosomal participation in the translocations and the existence of specific vulnerable points on chromosomes 1, 3, and 16 are suggested.

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