scholarly journals Effect of gonadotropins and alpha 2u-globulin on testicular steroidogenesis and spermatogenesis in melatonin-treated rats

2012 ◽  
Vol 8 (1) ◽  
pp. 7-12
Author(s):  
R Biswas ◽  
NM Biswas ◽  
H Mandal ◽  
NM Biswas
Keyword(s):  
Serum Level ◽  
Hydroxysteroid Dehydrogenase ◽  
Medical Sciences ◽  
Accessory Sex Organs ◽  
Testicular Steroidogenesis ◽  
Testicular Degeneration

Administration of melatonin (400?g/100g bd.wt.) for 14 days caused a fall in weights of the testes and accessory sex organs and testicular 17?-hydroxysteroid dehydrogenase (17?-HSD) but rise in 3?-hydroxysteroid dehydrogenase (3?-HSD) activity, decreased spermatogenesis, serum level of gonadotropins, testosterone and alpha 2u-globulin, The animals treated with melatonin when received gonadotropins or alpha 2u-globulin for the last seven days reversed the weight of testis and accessory sex organs, 3?-HSD, 17?-HSD activities, serum level of gonadotropins, testosterone and alpha 2u-globulin when compared with melatonin-treated rats. It is concluded that alpha 2u-globulin prevents testicular degeneration in melatonin-treated rats by stimulating the synthesis of gonadotropins. Journal of College of Medical Sciences-Nepal,2012,Vol-8,No-1, 7-12 DOI: http://dx.doi.org/10.3126/jcmsn.v8i1.6819

Studies on the effect of prolactin treatment on testicular steroidogenesis and gametogenesis in lithium-treated rats

1991 ◽  
Vol 125 (3) ◽  
pp. 313-318 ◽  
Author(s):  
D. Ghosh ◽  
N. M. Biswas ◽  
P. K. Ghosh
Keyword(s):  
Lithium Chloride ◽  
Lithium Treatment ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Significant Inhibition ◽  
Steroidogenic Enzymes ◽  
Subcutaneous Injections ◽  
Testicular Dysfunction ◽  
Testicular Steroidogenesis ◽  
Chloride Treatment

Abstract. The effect of PRL supplementation in lithium-treated rats on spermatogenesis, testicular Δ5-3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase activities, and serum levels of FSH, LH, PRL and testosterone were studied on the 22nd day of the experiment. Subcutaneous injections of lithium chloride at a dose of 2.0 mg·kg−1·day−1 for 21 days resulted in a significant inhibition of spermatogenesis at stage VII of the seminiferous epithelial cycle, along with remarkable diminution of serum levels of the above hormones and suppression of the activities of the above two testicular steroidogenic enzymes. Administration of bovine PRL at a dose of 0.25 mg·kg−1·day−1 plus lithium treatment resulted in a remarkable protection of spermatogenic and steroidogenic activities of the testes, along with restoration of serum levels of FSH and testosterone. It is concluded that PRL can markedly protect the testicular dysfunction induced by lithium chloride treatment in rats.

Biopotency and site of action of drugs affecting testicular steroidogenesis

1987 ◽  
Vol 113 (3) ◽  
pp. 457-461 ◽  
Author(s):  
A. Lambert ◽  
R. Mitchell ◽  
W. R. Robertson
Keyword(s):  
Cyproterone Acetate ◽  
Leydig Cells ◽  
Hydroxysteroid Dehydrogenase ◽  
Hormone Stimulation ◽  
Testosterone Secretion ◽  
Testicular Cells ◽  
Adrenal Steroidogenesis ◽  
Site Of Action ◽  
Testicular Steroidogenesis

ABSTRACT The effect of etomidate (an anaesthetic), epostane (WIN 32729; an inhibitor of ovarian and adrenal steroidogenesis) and cyproterone acetate (an antiandrogen) on testosterone secretion from mouse Leydig cells stimulated with LH (5 i.u./l) was tested. The concentration of drug which inhibited testosterone secretion by 50% was 11·5±1·1 (s.e.m.) μmol/l for cyproterone acetate, 1·2 ± 0·2 μmol/l for etomidate and 0·23 ± 0·03 μmol/l for epostane. The effect of all three drugs on testicular steroidogenesis was completely reversible. Thus testicular cells which had been washed after exposure to a >95% inhibitory dose of drug responded in a similar manner to hormone stimulation as cells similarly washed and which had not been exposed to the drug. The sites of the antisteroidogenic effect of epostane, etomidate and cyproterone acetate were established using a method based on the sequential stimulation by the exogenous precursor steroids of the various steps leading to the biosynthesis of testosterone. It was concluded that etomidate acts at the sequence between LH binding and pregnenolone production, epostane acts at 3β-hydroxysteroid dehydrogenase and cyproterone acetate inhibits 3β-hydroxysteroid dehydrogenase and C17,20-lyase. J. Endocr. (1987) 113, 457–461

Effect of α2u-globulin on serum concentration of gonadotrophins and testicular activity in oestrogen-treated rats

1983 ◽  
Vol 96 (2) ◽  
pp. 321-327 ◽  
Author(s):  
N. M. Biswas ◽  
P. K. Ghosh ◽  
K. K. Ghosh ◽  
O. W. Neuhaus
Keyword(s):  
Serum Concentration ◽  
Adult Male ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Organ Weight ◽  
Male Rats ◽  
Testicular Function ◽  
Accessory Sex Organs ◽  
Testosterone Synthesis

Adult male rats were given injections of oestradiol-17β (50 μg/100 g body wt per day) for 7 days. When they were killed 14 days after the last injection, serum levels of gonadotrophins and testosterone and weights of accessory sex organs were less, testicular 17β-hydroxysteroid dehydrogenase 17β-HSD activity was suppressed and spermatogenesis was inhibited. Administration of α2u-globulin (1·5 mg/day) for 14 days to oestrogen-treated rats and for 10 days to control rats resulted in increased concentrations of gonadotrophins and testosterone in the serum. Accessory sex organ weight and spermatogenesis appeared to be normal while 17β-HSD activity increased in oestrogen-treated rats after treatment with α2u-globulin. It was concluded that α2u-globulin has an effect on testicular function in oestrogenized rats by inducing gonadotrophin and testosterone synthesis.

scholarly journals Selective Inhibition of Steroidogenic Enzymes by Ketoconazole in Rat Ovary Cells

2014 ◽  
Vol 8 ◽  
pp. CMRH.S14036 ◽  
Author(s):  
Michael Gal ◽  
Joseph Orly
Keyword(s):  
Selective Inhibition ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
Steroidogenic Enzymes ◽  
Ovary Cells ◽  
Estrogen Synthesis ◽  
Lyase Activity ◽  
Testicular Steroidogenesis ◽  
Rat Ovary

Objective Ketoconazole (KCZ) is an anti-fungal agent extensively used for clinical applications related to its inhibitory effects on adrenal and testicular steroidogenesis. Much less information is available on the effects of KCZ on synthesis of steroid hormones in the ovary. The present study aimed to characterize the in situ effects of KCZ on steroidogenic enzymes in primary rat ovary cells. Methods Following the induction of folliculogenesis in gonadotropin treated rats, freshly prepared ovarian cells were incubated in suspension for up to four hours while radiolabeled steroid substrates were added and time dependent generation of their metabolic products was analyzed by thin layer chromatography (TLC). Results KCZ inhibits the P450 steroidogenic enzymes in a selective and dose dependent manner, including cholesterol side-chain cleavage cytochrome P450 (CYP11A1/P450scc), the 17α-hydroxylase activity of CYP17A1/P450c17, and CYP19A1/P450arom, with IC50 values of 0.3, 1.8, and 0.3 μg/mL (0.56, 3.36, and 0.56 μM), respectively. Unaffected by KCZ, at 10 μg/mL, were the 17,20 lyase activity of CYP17A1, as well as five non-cytochrome steroidogenic enzymes including 3β-hydroxysteroid dehydrogenase-δ5-4 isomerase type 1 (3βHSD1), 5α-reductase, 20α-hydroxysteroid dehydrogenase (20α-HSD), 3α-hydroxysteroid dehydrogenase (3α-HSD), and 17β-hydroxysteroid dehydrogenase type 1 (17HSD1). Conclusion These findings map the effects of KCZ on the ovarian pathways of progestin, androgen, and estrogen synthesis. Hence, the drug may have a potential use as an acute and reversible modulator of ovarian steroidogenesis in pathological circumstances.

Inhibition of pituitary gonadotropic activity in the viviparous seaperch Cymatogaster aggregata Gibbons by a dithiocarbamoylhydrazine derivative (I.C.I. 33 828)

1968 ◽  
Vol 46 (4) ◽  
pp. 751-758 ◽  
Author(s):  
John P. Wiebe
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Gonadal Hormones ◽  
State Characteristic ◽  
Testicular Steroidogenesis ◽  
Aquarium Water ◽  
Breeding Condition ◽  
Gonadotropic Activity ◽  
And Control ◽  
Immature Fish ◽  
Thiourea Treatment

A suspension of Methallibure (I.C.I. Compound 33 828) was added regularly for about 6 weeks to aquaria housing males of the teleost Cymatogaster aggregata. The testis atrophied and the gametogenetic processes of maturation were suspended. Histochemically demonstrable 3β-hydroxysteroid dehydrogenase and sudanophilic lipids diminished and the interstitial cells of Leydig regressed. At the end of the experiments the fleshy modifications on the male anal fin (secondary sex structures which develop under the influence of male gonadal hormones) were in a state characteristic of immature fish. Methallibure treatment resulted in these changes, which were characteristic of hypophysectomy, without the marked antithyroid activity histologically evident in thiourea treatment. All thiourea-treated and control animals remained in breeding condition. It is concluded that the addition of Methallibure to the aquarium water will directly block the production and (or) release of pituitary gonadotropins and thus inhibit spermatogenesis and testicular steroidogenesis.

Effect of lithium chloride on testicular steroidogenesis and gametogenesis in immature male rats

1991 ◽  
Vol 124 (1) ◽  
pp. 76-82 ◽  
Author(s):  
P. K. Ghosh ◽  
N. M. Biswas ◽  
D. Ghosh
Keyword(s):  
Lithium Chloride ◽  
Sperm Count ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Significant Inhibition ◽  
Male Rats ◽  
Hormonal Changes ◽  
Subcutaneous Injections ◽  
Immature Male ◽  
Testicular Steroidogenesis

Abstract. The present study was performed on immature male rats aged 35 days. Subcutaneous injections of lithium chloride at a daily dose of 2.0 mg/kg for 15 days resulted in significant inhibition of spermatogenesis at stage VII of the seminiferous epithelial cycle. Spermatogonia A, preleptotene spermatocytes and step 7 spermatids were decreased in number in comparison to controls. Serum levels of FSH, LH, PRL, and testosterone were decreased. Activities of testicular Δ5-3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase were suppressed along with a low caudal epididymal sperm count in comparison with controls. When the treatment was prolonged for 20 and 25 days, it showed an additional significant diminution in accessory sex organ weights and number of midpachytene spermatocytes at stage VII in comparison to control animals of corresponding age. It is concluded that lithium has an adverse effect on testicular function in immature rats by reducing serum levels of FSH, LH, PRL, and testosterone. Furthermore, since hormonal changes and altered spermatogenic activities were evident when the serum concentration of lithium was within the therapeutic range, our data may have some potential clinical implications.

Effect of dihydrotestosterone on serum concentrations of α2u-globulin and on spermatogenesis in melatonin-treated rats

1990 ◽  
Vol 126 (3) ◽  
pp. 431-435 ◽  
Author(s):  
H. Mandal ◽  
P. K. Ghosh ◽  
N. M. Biswas
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Male Rats ◽  
Testicular Function ◽  
Serum Concentrations ◽  
Melatonin Treatment ◽  
Accessory Sex Organs

ABSTRACT Adult male rats were given s.c. injections of melatonin (400 μg/100 g body weight per day) for 14 days. On day 15, the weights of the testis and accessory sex organs were less, testicular 17β-hydroxysteroid dehydrogenase (17β-HSD) activity was inhibited, spermatogenesis was suppressed and serum levels of gonadotrophins, testosterone and α2u-globulin were decreased compared with control animals injected with vehicle. In a third group of rats given the same dose of melatonin for 14 days, administration of dihydrotestosterone (DHT) at a dose of 25 μg/100 g body weight per day on days 8–14 resulted in serum levels of α2u-globulin, FSH, LH and testosterone and testicular 17β-HSD activity similar to those seen in vehicle-injected control animals. Weights of the testes and accessory sex organs and spermatogenesis were normal after administration of DHT in melatonintreated rats. In another group of rats, the depressive effects of melatonin treatment on plasma gonadotrophins were reversed by the administration of α2u-globulin on days 8–14. It was concluded that treatment with DHT prevents the depressive action of melatonin on testicular function by inducing the synthesis of α2u-globulin. Journal of Endocrinology (1990) 126, 431–435

scholarly journals Serum Level of Anti-Hepatitis B Surface Antigen 18 Years after Vaccination in Students of Golestan University of Medical Sciences, Iran

2021 ◽  
Vol 5 (1) ◽  
pp. 31-36
Author(s):  
Rokhsare Ebneghasem ◽  
Sima Besharat ◽  
Behnaz Khodabakhshi ◽  
Roghieh Golsha ◽  
Hessamaddin Shirzad-Aski ◽  
...  
Keyword(s):  
Serum Level ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Medical Sciences
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