scholarly journals Inhibition of Salmonella Typhimurium by medium chain fatty acids in an in vitro simulation of the porcine caecum

2007 ◽  
Author(s):  
J. Goris ◽  
N. Dierick ◽  
L. Herman ◽  
M. Heyndrickx ◽  
W. Messens
Keyword(s):  
Fatty Acids ◽  
Salmonella Typhimurium ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
In Vitro Simulation ◽  
Chain Fatty Acids

Inhibition of Salmonella typhimurium by medium-chain fatty acids in an in vitro simulation of the porcine cecum

2010 ◽  
Vol 141 (1-2) ◽  
pp. 73-80 ◽  
Author(s):  
Winy Messens ◽  
Johan Goris ◽  
Noël Dierick ◽  
Lieve Herman ◽  
Marc Heyndrickx
Keyword(s):  
Fatty Acids ◽  
Salmonella Typhimurium ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
In Vitro Simulation ◽  
Chain Fatty Acids

scholarly journals Use of Medium Chain Fatty Acids To Mitigate Salmonella Typhimurium (ATCC 14028) on Dry Pet Food Kibbles

2020 ◽  
Vol 83 (9) ◽  
pp. 1505-1511
Author(s):  
JANAK DHAKAL ◽  
CHARLES G. ALDRICH
Keyword(s):  
Fatty Acids ◽  
Salmonella Typhimurium ◽  
Pet Food ◽  
Treatment Control ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Dog Food ◽  
Evaluation Time ◽  
Time Point ◽  
Chain Fatty Acids

ABSTRACT This study evaluated the antimicrobial effects of medium chain fatty acids (MCFAs) against Salmonella Typhimurium (ATCC 14028) when used on dry dog food kibbles. The MIC of three MCFAs, caproic (C6), caprylic (C8), and capric (C10), was determined using the broth micro- and macrodilution assay technique. Using canola oil as a fat coating, the efficacy of each MCFA was then tested on dry dog food kibbles at 37°C for up to 5 h. The MIC was found to be 0.3125, 0.3125, and 0.625% for C6, C8, and C10, respectively. When the MCFAs were tested on fat-coated dry kibbles, all three MCFAs reduced (P ≤ 0.05) Salmonella levels by >4.5 log after 5 h when the Salmonella recovery from a no-treatment control was ∼6.4 log. At each evaluation time point, the three treatments were effective in reducing (P ≤ 0.05) Salmonella loads. No countable colonies of Salmonella were detected at 4 h when the combination of C6+C8 was used on the kibbles (P ≤ 0.05), whereas with the C6+C10 combination, the Salmonella colonies were not detectable between 2 and 4 h after treatments (P ≤ 0.05). Different combinations of C8 and C10 caused Salmonella to drop to a nondetectable limit (1 CFU/g) between 1 and 5 h after treatment (P ≤ 0.05). This study suggests that the use of MCFAs during kibble coating may mitigate postprocessing Salmonella recontamination on dry dog food kibbles. HIGHLIGHTS

scholarly journals In vitro antimicrobial effect of palm oils rich in medium-chain fatty acids against mastitis-causing Gram-positive bacteria

2019 ◽  
Vol 64 (No. 8) ◽  
pp. 325-331
Author(s):  
Klára Laloučková ◽  
Lucie Malá ◽  
Paula Slaničková ◽  
Eva Skřivanová
Keyword(s):  
Fatty Acids ◽  
Dairy Cattle ◽  
Antibacterial Effect ◽  
Bovine Mastitis ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Streptococcus Uberis ◽  
Broth Microdilution Method ◽  
Chain Fatty Acids

Various pathogens causing mastitis in dairy cattle are of serious concern due to their increasing antibacterial resistance and potential transmission to other cows, calves, and the environment, especially through the milking process. Therefore, alternative approaches to antimicrobial usage in the treatment or control of mastitis in dairy cattle are severely needed. The antibacterial effect of medium-chain fatty acids (MCFAs) is known to be significant for various pathogens, but there is only limited information about the activity of MCFAs on mastitis-causing pathogens. Moreover, no evidence about the antimicrobial effects of palm oils rich in MCFAs, such as coconut, palm kernel, and tucuma oil, can be found in the current literature. The aim of this study was to evaluate the in vitro antibacterial effect of palm oils rich in MCFAs, after cleavage by an exogenous lipase from Mucor javanicus, on bovine mastitis-causing strains (Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis) by the broth microdilution method. All tested palm oils exerted antibacterial activity against eight tested bacterial strains in the range of 64–8192 µl/ml with Str. agalactiae being the most sensitive and S. aureus being the most resistant species. The results of the present study demonstrate that palm oils rich in MCFAs can serve as an alternative to the predominantly used predip and postdip procedures in bovine mastitis control, but further in vivo studies are needed to confirm the findings for their possible applications.

scholarly journals The potential anti- African Swine Fever Virus effects of medium chain fatty acids on in vitro pig feed and water models

2020 ◽  
Author(s):  
Ha Thi Thanh Tran ◽  
Anh Duc Truong ◽  
Duc Viet Ly ◽  
Tuan Van Hoang ◽  
Chinh Thi Nguyen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Control Measures ◽  
Effective Control ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Water Models ◽  
Chain Fatty Acids

Abstract Background African swine fever (ASF) is an important disease affecting swine and has a significant economic loss on both the developed and developing world but due to the lack of vaccines, drug, and effective control measures, ASF virus remains a serious threat to global pork production. The activities of medium-chain fatty acids (MCFAs) against viral pathogens have been reported previously. However, the effects of this family on ASFV have been not yet investigated. In this study, we evaluated the potential effects of MCFAs in individual and synergistic forms, to prevent and/or reduce ASFV infection using in vitro feed and water models. Results The potential effects of MCFAs, including C8, C6-C8-C10 (1:1:1 ratio) and C8-C10-C12 (1:1:1 ratio) against a field ASFV strain isolated in Red River Delta region of Vietnam were further examined by real-time PCR in in vitro feed and water models. All tested products have shown a strong antiviral effect against ASFV infectivity at doses of 0.375% and 0.5% in both feed and water assays. Interestingly, the synergistic MCFAs have shown clearly their potential activities against ASFV in which at lower dose of 0.25%, pre-treatment with product 2 and 3 induced significant increases at the level of Cq value when compared to positive control and/or product 1 (P < 0.05). Conclusions To our knowledge, it is the first report on in vitro examination of the anti-ASFV activities of the MCFAs. Our findings suggested that all tested products, both individual and synergistic forms of MCFAs, have possessed a strong anti-ASFV effect and this effect is dose- dependence in in vitro feed and water models. Additionally, synergistic effects of MCFAs are more effective against ASFV when compared to individual form. The further studies focusing on in vivo anti-ASFV effects of MCFAs are very important to bring new insight into the mode of ASFV-reduced action by these compounds in swine feed and water consumption.

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