Synthesis of symmetrical structured triglycerides via a bottom-up process

The synthesis of symmetrical structured triglycerides (STG) through a bottom-up approach was previously shown to produce 1,3-dioleoyl-2-palmitoyl glycerol in significant quantities. This solvent-free lipase-catalyzed process, consisting of a low-temperature (40 °C) esterification step with glycerol dosing followed by a high-temperature (60 °C) esterification step, was further investigated in the production of symmetrical medium-and-long-chain triglycerides (MLCT). By replacing oleic acid with capric acid in the first step or the palmitic acid by either capric acid or lauric acid in the second step, the effects of free fatty chain length and sequence of fatty acid addition on STG production were established. These produced 1,3-dicaproyl-2-oleoyl glycerol, 1,3-dioleoyl-2-caproyl glycerol, and 1,3-dioleoyl-2-lauroyl glycerol at concentrations of 36.98 g, 36.77 g, and 37.08 g per 100 g of triglycerides respectively after 72 h at an overall FFA1:FFA2:Glycerol of 2:1:1 and 4 g Novozyme 435 per 100 g reactants, without the purification of intermediates and products. The sequence of fatty acid addition had the most significant effect as purer STG products can be obtained when the medium chain fatty acid is introduced in the first step. As the process was carried out without solvents, the STG produced are appropriate for functional food or nutraceutical applications.

Applied Studies on Application of Capric/Caprylic MCT Oils in Food Industries

Non healthy oils & fats consumption in foods is the major reason of obesity in human beings. Common cooking oils & fats are composed of medium and long chain triglycerides. Each triglyceride consist of fatty acids called medium and long chain fatty acids abbreviated as (MCFAs & LCFAs). The ratio of LCFAs is mostly higher than the MCFAs in common cooking oils to be to be suitable for heat stress applications like cooking and frying. On the other side there is natural dietary fats are rich in Medium-chain fatty acids (MCFAs) like coconut oil and dairy fats. Also there are synthetic medium-chain triglyceride (MCT) oils which are synthetized by a processes called fractionation that extracts the MCFAs as caprylic and capric acid from the other fatty acids in the coconut or palm kernel oil. The MCFAs, shows substantial metabolic advantage compared to LCFAs. MCFAs are a preferred source of energy (b-oxidation). The last 20 years studies confirmed the potential of MCFAs to reduce body weight and may reduce fasting lipid levels more than oils rich in LCFAs. The same is true for glucose levels. In this study, the authors did chemical and instrumental studies on dietary structured MLCT and non-structured MCT/LCT cooking oils which were used for producing for weight reduction and obesity control purpose Capric /caprylic MCT oil was used as a source of MCFAs for producing of novel. Different sources of long-chain triglycerides (LCFAs) have been used including sunflower oil (SFO), Canola oil(CNO), high oleic sunflower oil (HOSFO), palm oil (PO) and double fractionated palm olein (DFOlein). The following techniques have been used for preparation of structured and non structured cooking oils in this study : 1-Physical blending. 2 - Chemical interesterification in a multipurpose batch reactor. 2- Enzymatic interesterification using immobilized Lipase enzyme in pilot scale packed bed reactor (PBR). Different analytical and instrumental techniques were used in this study including Gas chromatography (GC), high-performance liquid chromatography (HPLC), Differential scanning calorimeter (DSC), Rancimate, and smoke point tester. The study shown that the non-structured MCT/LCT oils gave reasonable heat stability, higher smoke points compared the structured MLCT oil which make it more suitable for cooking and frying applications. The chemical and enzymatic interesterification (CIE & EIE) technique yield a real structured MLCT oil which is more effective in caloric reduction and obesity control purpose during long term consumption in dietary foods, however, due to its much lower smoke point compared to its relative LCFAs oil so it will be suitable only for cold applications like salad dressing and other culinary applications. But not suitable for heat stress applications like deep or shallow frying.

An effective medical replacement therapy: ketogenic diet for intractable childhood epilepsy

Ketogenic diet (KD) a high fat, adequate protein and low carbohydrate restrictive diet has a long history of its use in intractable epilepsy of childhood. The diet produces biochemical changes mimicking that of starvation. The high levels of ketone bodies produced by KD act as a major source of energy for brain replacing the usual glucose.1 Comprising the ratio of 4:1 (fat:carbohydrate and protein) by weight, the diet produces state of ketonemia or ketosis that leads to reduction in frequency of epileptic seizures by is unique mode of action. To increase the palatability medium chain triglycerides (as coconut oil) in ratio of 3:1 is used which is more efficiently absorbed and have lesser gastro intestinal side effects as compared to traditional 4:1 ratio diet with long chain triglycerides like PUFA

Lipolytic Activity of a Carboxylesterase from Bumblebee(Bombus ignitus) Venom

Bee venom is a complex mixture composed of peptides, proteins with enzymatic properties, and low-molecular-weight compounds. Although the carboxylesterase in bee venom has been identified as an allergen, the enzyme’s role as a venom component has not been previously elucidated. Here, we show the lipolytic activity of a bumblebee (Bombus ignitus) venom carboxylesterase (BivCaE). The presence of BivCaE in the venom secreted by B. ignitus worker bees was confirmed using an anti-BivCaE antibody raised against a recombinant BivCaE protein produced in baculovirus-infected insect cells. The enzymatic activity of the recombinant BivCaE protein was optimal at 40 °C and pH 8.5. Recombinant BivCaE protein degrades triglycerides and exhibits high lipolytic activity toward long-chain triglycerides, defining the role of BivCaE as a lipolytic agent. Bee venom phospholipase A2 binds to mammalian cells and induces apoptosis, whereas BivCaE does not affect mammalian cells. Collectively, our data demonstrate that BivCaE functions as a lipolytic agent in bee venom, suggesting that BivCaE will be involved in distributing the venom via degradation of blood triglycerides.

Nutritional Values and Health Protective Properties Of Coconut Oil

Chemically, fat or oil is a mixture of triacylglycerol molecules, in which glycerol esterified with three fatty acids. Fatty acid is a monocarboxilic acid containing even number of carbon atom started from 4 to 22. Based on the length of fatty acid in triacylglycerol, fats and oils can be classified into two groups; medium chain triglycerides and long chain triglycerides. Coconut oil belongs to medium chain triglycerides oil because it’s fatty acids consist mostly of medium chain fatty acids (C4:0 to C12:0) and dominated by lauric acid (C12:0), hence usually called as lauric oil. In the year of 1950s, coconut oil was claimed that saturated fats, including coconut oil, could increase blood total cholesterol and hence is atherogenic, while unsaturated fats decrease total cholesterol. However, in 1990s, coconut oil was found to be different from the other saturated oils. Coconut oil composed of medium chain fatty acids with high amount of lauric acid. Coconut oil is metabolized differently from long chain triglycerides saturated oil, and therefore coconut oil has numerous beneficial nutritional values and health promotion. Consumption of food rich in medium chain fatty acids reduces the level of body fat and the decrease the risk of heart disease, diabetes, increase mother’s milk quality and active as potential antibacterial agent.

INJECTION PAIN OF PROPOFOL IN CHILDREN WITH MEDIUM PLUS LONG CHAIN TRIGLYCERIDES AND LONG CHAIN PROPOFOL MIXED WITH LIGNOCAINE

Introduction: Propofol is widely accepted drug because of its rapid and smooth induction, short context-sensitive time, rapid terminal half-life time and low incidence of postoperative nausea and vomiting. It is used for sedation and anaesthesia for almost all types of surgeries. During a propofol injection, pain due to the long-chain triglyceride (LCT) emulsion is experienced by 70% of adults and up to 85% of children. Despite various strategies to reduce propofol injection pain, this still poses a clinical problem in adults and children, and the incidence reported to be 30–90%. A medium-chain triglyceride/long-chain triglyceride (MCT/LCT) emulsion has been advised to reduce injection pain as compared to propofol LCT in adults and teenagers Material and Methods: 100 children aged 5–13 years, in American Society Anaesthesiologists (ASA) class I or II, undergoing elective general anaesthesia without any contraindication to propofol anaesthesia were included in the study. Injection pain following injection propofol was assessed in all patients, but the anaesthetist was blinded for which formula was being used. Results: The demographic characteristics like age, sex, weight, amount of propofol given, ASA physical status and recovery period was calculated. No statistically significant difference was observed in both the groups. Incidence of pain and severe pain in LCT group was 24 (48%) and 7 (14%) respectively while incidence of pain and severe pain in MCT/LCT group was 15 (30%) and 1 (2%) respectively. Pain score was found less in group MCT / LCT. Conclusion: Medium plus long chain triglycerides and long chain propofol along with lignocaine significantly reduces the incidence as well as the severity of injection pain in the paediatric age group and can be used in children for sedation or induction of anaesthesia.