scholarly journals The Investigation of Some Phytochemical Compounds Found in Anchusa strigosa L. Grown Naturally in Iraq

2021 ◽  
Vol 30 (1) ◽  
pp. 179-188
Author(s):  
Suroor A. Ghalib ◽  
Enass J. Kadhim
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fourier Transforms ◽  
Ftir Spectra ◽  
Perennial Herb ◽  
Absolute Methanol ◽  
Phytochemical Study ◽  
Soxhlet Apparatus ◽  
Layer Chromatography ◽  
Preparative Layer Chromatography

Anchusa strigosa L.: Hardy annual biennial or perennial herb, with hairs especially on the leaves., flowers generally regular. Commonly named (Lisan Althour) in Iraq, from Boraginaceae family. The plant contains phenolic acids, flavonoids, alkaloids, sterols, and terpenoids. The Whole plant part defatted with n-hexane for 24 hours. The defatted plant material extracted using absolute methanol by Soxhlet apparatus for 24 hours, the extract fractionated by solvents of different polarity: petroleum ether- chloroform - ethylacetate- and n-butanol respectively. The n-butanol fraction hydrolyzed with 10% HCl for 5 hours by reflex to break down the glycosidic linkage. Rosmarinic acid, caffeic acid, genistein, and silybin were isolated from ethyl acetate fraction by preparative layer chromatography which identified by high performance liquid chromatography HPLC, Fourier transforms infrared (FTIR) spectra, thin- layer chromatography TLC and melting point. Since the plant contain alkaloids so acid- base extraction performed for crude extract resulting from the maceration of the plant parts in methanol (cold method) to obtain the alkaloid that isolated by preparative layer chromatography and then identified by Fourier transforms infrared (FTIR) spectra and thin-layer chromatography (TLC). The aim of this research was to carry out a phytochemical study of this plant since no previous phytochemical investigation work had been done on this species in Iraq.

scholarly journals phytochemical investigations of Iraqi Abrus precatorius Linn. plant

2018 ◽  
Vol 27 (1) ◽  
pp. 30-38
Author(s):  
Zahra'a S. Nassir ◽  
Enas J. Khadem
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Fourier Transforms ◽  
Ethanolic Extract ◽  
Preparative Thin Layer Chromatography ◽  
Chloroform Fraction ◽  
Abrus Precatorius ◽  
Soxhlet Apparatus ◽  
Layer Chromatography

    The plant Abrus precatorius, which belong to Leguminosae (Fabaceae) family and known as Crab’s eyes, Rosary pea with characteristic red and black seeds. It was used in folk medicine in India, China and East Asian countries for treatment of various diseases.    The plant was extracted by '' general method of extraction'' (Harborne, 1973) using 80% aqueous ethanol as a solvent of extraction by soxhlet apparatus. Preliminary qualitative phytochemical screening were performed on the crude ethanolic extract  and revealed the presence of alkaloids, flavonoids ,terpenoids and phytosterols in Iraqi Abrus precatorius plant. Three different fractions were obtained from crude extract which are fraction one (chloroform fraction), fraction two (ethyl acetate fraction), and fraction three (petroleum ether fraction) which are represent alkaloids, flavonoids and steroids respectively. The alkaloid abrine was isolated from the chloroform fraction  in pure form by using preparative thin layer chromatography (PTLC) and then subjected to different physico-chemical and specteral analytical techniques to identify its chemical structure: melting point (M.P.), thin layer chromatography (TLC), high performance liquid chromatography (HPLC) , fourier transforms infrared spectra (FT-IR) and elemental microanalysis (CHNO).      

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

Qualitative analysis of the herb fagopyrum sagittatum gilib grown in pot culture

2020 ◽  
pp. 63-68
Author(s):  
A. S. Soldatov ◽  
N. V. Nesterova ◽  
N. V. Biryukova
Keyword(s):  
Qualitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phytochemical Study ◽  
Promising Source ◽  
Layer Chromatography ◽  
Pot Culture

This article is devoted to the phytochemical study of fresh and dried samples of buckwheat grass, the preparation of which was carried out from plants cultivated in pot culture using pharmacopoeial qualitative reactions. The presence of tannins and flavonoids was identified, and using the method of thin-layer chromatography, using GSO Rutin, rutin was identified, which allows us to consider potted buckwheat culture as a promising source of rutin production.

scholarly journals DENSITOMETRIC VALIDATION OF LAPACHOL IN TECOMELLA UNDULATA SEEM BARK BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

2018 ◽  
Vol 10 (7) ◽  
pp. 87
Author(s):  
Raj Richa ◽  
Siddiqui Nahida ◽  
Aeri Vidhu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Quantitative Estimation ◽  
Marker Compound ◽  
Soxhlet Apparatus ◽  
Peak Areas ◽  
Tecomella Undulata ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: To develop a validated high-performance thin layer chromatography (HPTLC) method for the quantitative estimation of Lapachol in a Soxhlet extracted bark of Tecomella undulata seem (T. undulata). Methods: The bark of T. undulata was extracted with chloroform by Soxhlet apparatus. The separation was achieved on a silica-gel 60 F254 HPTLC plate using toluene-ethyl acetate–glacial acetic acid (8.5:1.5:0.02 v/v/v) as a mobile phase. Densitometric analysis of Lapachol was carried out in absorbance mode at 254 nm.Results: The proposed method was accurate for the separation and resolution between peaks of the standard and Lapachol (5.04µg) with Rf value 0.77. Calibration curves were found to be linear over the concentration range (10-130µg) for Lapachol and correlation coefficient over (R2= 0.9973), indicating an excellent correlation between peak areas and concentrations of the marker compound. The experimentally derived LOD and LOQ for Lapachol were determined to be 0.028 µg and 0.086 µg respectively and the developed HPTLC-UV method showed lower %RSD and SEM, value indicating the method to be precise, accurate and robust.Conclusion: The study concludes that HPTLC-UV validation method can be very efficient and promising technique for the identification and quantitative analysis of Lapachol from T. undulata bark. The statistical analysis of data indicates that the developed method is reproducible and specific.

scholarly journals Phytochemical Screening of Sorghum (Sorghum Sp) with Thin Layer Chromatography Method

2020 ◽  
Vol 3 (2) ◽  
pp. 8-12
Author(s):  
Endang Noerhartati ◽  
Muhammad Farid Rizal ◽  
Elika Joeniarti
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Glycemic Index ◽  
Methanol Extract ◽  
Pharmaceutical Products ◽  
Absolute Methanol ◽  
Phytochemical Screening ◽  
Chromatography Method ◽  
Layer Chromatography ◽  
Low Glycemic Index

Sorghum is a food plant rich in benefits and has the advantage of being free gluten; it contains antioxidants and has a low glycemic index. The purpose of this research is to determine the content of compounds in sorghum. In the research, the variety of KD 4 sorghum has been used, which was macerated by absolute methanol. Results screening using thin-layer chromatography showed that methanol extract of sorghum contained flavonoid, phenol, triterpenoid, tannin, and saponin compounds, and finally, these compounds will be useful for pharmaceutical products.

scholarly journals A Pharmacognostical and Phytochemical study of Sookshma Eladi Choornam

2020 ◽  
Vol 11 (4) ◽  
pp. 712-715
Author(s):  
Harinatha Chary B ◽  
Manu Rajagopal ◽  
Pavan Kumar S ◽  
Gnana Prasuna S
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Large Scale ◽  
Fine Powder ◽  
Chemical Study ◽  
Quality Parameters ◽  
Water Soluble ◽  
Dissolution Time ◽  
Phytochemical Study ◽  
Layer Chromatography

Introduction: Sookshma Eladi choornam is one of the Ayurvedic formulations specially mentioned for Kaphaja Hridroga in Hridroga prakarana of Bhaishajya Ratnavali.  Dhamani pratichaya (Atherosclerosis) is one of the diseases of Kaphaja nanatmaja vaydhi which is the more important cause for Ischemic Heart Disease. The cause for Atherosclerosis could be Hyperlipidemias. Eladi choornam is a compound preparation which contains powders of Sookshma ela (Elettaria cardamomum Maton.) and Pippali moolam (root of Piper longum L.,). As these drugs are Katu vipaka in nature they can be used in Hyerlipidemia.  To know the efficacy of the drugs their quality parameters are highly essential to manufacture in the large scale. Method: The present study deals with the Pharmacognostical and phyto-chemical study of Sookshma eladi choornam including Thin Layer chromatography study (TLC) as per the standard literature. Result: Consistency of Sookshma eladi choornam is fine powder. Colour was brown, odour was aromatic spicy smell, Taste was pungent, touch was smooth powder form,  Qualitative study showed that pH is 4.9, total ash value 12 %, loss on drying is 4.5%, Water soluble matter17%, Alcohol soluble matter 8%,  Acid insoluble ash 8.5%. Thin Layer chromatography (TLC) revealed one yellow spot.  Dissolution time is 4 minutes and Moisture content was 8%. Phytochemicals as Alkaloids, Glycosides, Tannins, Flavonoids and Phenols were found. Conclusion: Pharmacognostical, phyto-chemical and TLC studies inferred that the formulation meets the minimum quality standards. The study may be used as reference standard in the further quality control researches.

scholarly journals Synthesis of quaternary ammonium salts based on quinuclidin-3-ol and pyridine-4-aldoxime with alkyl chains

St open ◽  
2020 ◽  
Vol 1 ◽  
pp. 1-8
Author(s):  
Doris Crnčević ◽  
Renata Odžak
Keyword(s):  
Melting Point ◽  
Nitrogen Atom ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antimicrobial Agents ◽  
Ftir Spectra ◽  
Ammonium Salts ◽  
Product Yields ◽  
Alkyl Chains ◽  
Layer Chromatography

Aim: In search of a new class of potential antimicrobial agents, novel ammonium salts based on quinuclidine-3-ol and pyridine-4-aldoxime with different lengths of alkyl chains were synthesized and analyzed. In addition to their potential biological application, newly synthesized salts containing terminal bromine could possibly be used for the synthesis of more potent bisquaternary salts. Methods: The commercially available quinuclidine-3-ol and pyridine-4-aldoxime were used for the synthesis of new derivatives with the appropriate alkyl chains. The purity of the synthesized salts was tested by thin-layer chromatography on an aluminum plate where aluminum oxide was used as a stationary phase. Melting points were determined in open capillaries using an instrument for melting point determination and the obtained values were left uncorrected. The FTIR spectra were recorded with a spectrometer and the data were analyzed in cm-1. Results: All the synthesized compounds, which contained heterocyclic moiety and alkyl chains (with or without a terminal bromine atom), were obtained in very good yields under the simple quaternization conditions. The better product yields were observed in reactions with quinuclidine3-ol (46-95%) compared to those with pyridine-4-aldoxime (49-59%). The obtained products were analyzed and confirmed by the thin-layer chromatography, the melting point measurement and the FTIR spectra. Conclusion: The observed difference in product yields could be explained by the different basicity of the nitrogen atom of quinuclidin-3-ol than that of pyridine-4-aldoxime. All prepared salts have a positively charged nitrogen atom as a part of their polar “head” and a long hydrocarbon chain as the non-polar “tail”. Such a structure allows electrostatic interaction with the negatively charged bacterial membrane and causes it to be disrupted. The structure of the prepared salts containing long alkyl chains could be crucial for their antimicrobial activity.

Sign in / Sign up

Export Citation Format

Share Document