Fermentative Production of Melanin by the Fungus <i>Auricularia auricula</i> Using Wheat Bran Extract as Major Nutrient Source

Objective: The study was aimed to evaluate the hypolipidemic and antioxidant effect of Triticum aestivum (wheat) bran extraction cholesterol-fed rabbits.Methods: The induced hyperlipidemic rabbits were treated with 70% ethanolic extract of wheat bran at a dose of 500 mg/kg body weight/day. The experimental protocol was designed for control, hyperlipidemic, wheat bran extract, and statin-treated groups for 60 days. The estimations for serum lipid profile, lipid peroxidation (LPO), and ferric reducing ability of plasma (FRAP) were done, and histopathological study of thoracic aorta was carried out.Results: Oral administration of wheat bran extract caused significant reductions in the serum lipid profile by 94.30% (total cholesterol), 96.73% (low-density lipoprotein cholesterol), and 58.28% (triglycerides). It significantly decreased the LPO and enhanced the antioxidant capacity (FRAP) activity as compared to the hyperlipidemic rabbits. Histopathology revealed that wheat bran extract treatment prevented the accumulation of cholesterol and regressed atheromatous plaques significantly in the aorta as compared to the hyperlipidemic group, and the results were comparable to that of the standard drug statin.Conclusion: The study exhibited that wheat bran extract controls atherosclerosis just like statin and can be used as a regular dietary supplement to manage cholesterol levels and prevent cardiovascular problems.

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Role of the Inorganic Constituents of Wheat Bran Extract in Streptomycin Production

Nature ◽

10.1038/162850a0 ◽

1948 ◽

Vol 162 (4126) ◽

pp. 850-850 ◽

Cited By ~ 1

Author(s):

R. RAGHUNANDANA RAO

Keyword(s):

Wheat Bran ◽

Wheat Bran Extract ◽

Inorganic Constituents

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Fermentative Production of Hydrogen from Wheat Bran by Mixed Anaerobic Cultures

Industrial & Engineering Chemistry Research ◽

10.1021/ie701789c ◽

2008 ◽

Vol 47 (16) ◽

pp. 5812-5818 ◽

Cited By ~ 26

Author(s):

Chunmei Pan ◽

Yaoting Fan ◽

Hongwei Hou

Keyword(s):

Wheat Bran ◽

Fermentative Production ◽

Production Of Hydrogen

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Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota

10.1101/2021.07.23.453482 ◽

2021 ◽

Author(s):

Ana S Luis ◽

Arnaud Basle ◽

Dominic P Byrne ◽

Gareth SA Wright ◽

James London ◽

...

Keyword(s):

Gut Microbiota ◽

Functional Data ◽

Molecular Level ◽

Human Colon ◽

Carbohydrate Active Enzymes ◽

Nutrient Source ◽

Human Gut ◽

Human Gut Microbiota ◽

Host Health ◽

Major Nutrient

The vast microbial community that resides in the human colon, termed the human gut microbiota, performs important roles in maintaining host health. Sulfated host glycans comprise both a major nutrient source and important colonisation factors for this community. Carbohydrate sulfatases remove sulfate groups from glycans and are essential in many bacteria for the utilisation of sulfated host glycans. Additionally, carbohydrate sulfatases are also implicated in numerous host diseases, but remain some of the most understudied carbohydrate active enzymes to date, especially at the structural and molecular level. In this work, we analyse 7 carbohydrate sulfatases, spanning 4 subfamilies, from the human gut symbiont Bacteroides thetaiotaomicron, a major utiliser of sulfated host glycans, correlating structural and functional data with phylogenetic and environmental analyses. Together, these data begin to fill the knowledge gaps in how carbohydrate sulfatases orchestrate sulfated glycan metabolism within their environment.

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Optimizing Laccase Production in Auricularia Cornea by Submerged Fermentation With Wheat Bran Extract: Applications in Decolorization of Malachite Green Dye

10.21203/rs.3.rs-464777/v1 ◽

2021 ◽

Author(s):

Li Meng ◽

Xiaoran Bai ◽

Qingji Wang ◽

Xiaobo Li ◽

Shaoyan Zhang ◽

...

Keyword(s):

Malachite Green ◽

Wheat Bran ◽

Submerged Fermentation ◽

High Performance ◽

Laccase Activity ◽

Degradation Products ◽

Laccase Production ◽

Malachite Green Dye ◽

Chromosome Bridge ◽

Wheat Bran Extract

Abstract Wheat bran extract may enhance laccase activity of Auricularia cornea in submerged fermentation. The laccase activity of A. cornea was maximal (768.78 U/mL) at 26.9 ℃, pH 5, a time point of 6 d, 22 h, and an inducer concentration of 10%. Laccase from A. cornea AC5 decolorized 83.27% of 75 mg/L malachite green within 12 h. High performance liquid chromatography (HPLC) analysis of the extracted metabolites suggested that the decolorization occurred through biodegradation. Malachite green induced cytotoxic damage including formation of a micronucleus and chromosome bridge at anaphase. Degradation products of malachite green proved to be less toxic and had negligible effects on chromosomal aberrations.

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