scholarly journals Identification of short-stem gene Rht-B1 in the winter soft wheat samples

2020 ◽  
pp. 55-59
Author(s):  
N. T. Kupreyshvily ◽  
N. N. Vozhzhova ◽  
D. M. Marchenko ◽  
E. V. Ionova

The current paper has presented the estimation results of the collection material of winter soft wheat according to the presence of alleles of short-stem gene Rht-B1. Identification of the samples with short-stem genes is of great necessity in the development of winter wheat varieties of the steppe ecotype with high adaptability to harvesting. Search, identification and development of a new highly productive and short-stem source material of winter soft wheat is of great relevance nowadays. The purpose of our study was to identify alleles of short-stem gene Rht-B1 in the collection samples of winter soft wheat. The study was carried out in the laboratory of the marker breeding of the FSBSI “Agricultural Research Center “Donskoy” in 2017. There were used the following research methods: a DNA extraction by the CTAB-method, estimation of the quantity and quality of the extracted DNA on a spectrophotometer, fulfillment of polymerase chain reaction and agarose gel electrophoresis, detection of amplicons in ultraviolet by gel photodocumentation system. As a result, due to the use of SSR-markers there were studied 162 short-stem samples of winter soft wheat of various ecological and geographical origin. There were identified 7 winter soft wheat samples with the Rht-B1a allele, such as “Grom”, “Kraplina”, “Stepovichka”, etc. and 13 winter soft wheat samples with the Rht-B1b (Rht1) allele, such as “1765/13”, “1816/15”, “GK Margit”, etc. There were identified 9 winter soft wheat samples with the Rht-B1e (Rht11) allele, such as “GK Margit”, “Minestrel”, “1004/14”, etc. It was recommended to use winter soft wheat samples with the Rht-B1b and Rht-B1e alleles when breeding for short stems.

2020 ◽  
pp. 12-17 ◽  
Author(s):  
M. M. Ivanisov ◽  
D. M. Marchenko ◽  
E. I. Nekrasov ◽  
I. A. Rybas ◽  
T. A. Grichanikova ◽  
...  

The improvement of grain production is the most important global task. The introduction of new highly productive varieties of the leading grain crop, winter wheat, plays a huge role in its solution. The paper presents the study results of 80 winter soft wheat varieties of various ecological and geographical origin. Frost and winter resistance rise of the developed winter soft wheat genotypes today and in the nearest future is one of the main directions of breeding work. The preservation of highly productive winter soft wheat varieties varied from 0.0% to 78.3% at a freezing temperature of –19 °C. The varieties “Kaprizulya”, “Lilit”, “Zhavoronok”, “Lydia”, “Krasa Dona”, “Polina”, “Volnitsa” and “Volny Don”, developed in the Agricultural Research Center “Donskoy” have the highest frost resistance among the studied samples, they preserved 57.0 ... 78.3% of living plants. Grain productivity of the samples ranged from 6.36 to 8.90 t/ha in 2015–2017. The 16 out of 80 studied varieties reliably (НСР05 = 0.64 t/ha) exceeded the standard variety “Don 107” for this trait. Productivity of the best samples ranged from 8.14 t/ha (“СО 911”) to 8.90 t / ha (“Laureat”). The biological productivity of winter soft wheat varieties varied from 8.37 to 9.07 t/ha, exceeding the standard variety from 0.67 t/ha (“СО 911”) to 1.37 t/ha (“Laureat”). It should be noted that the correlation coefficient of actual and biological productivity was 0.84±0.06. As a result of the current study, there were identified the genotypes “Lydia”, “Volnitsa” and “Zhavoronok” combining high productivity, grain quality and resistance to low negative temperatures.


Author(s):  
Т.А. АСЕЕВА ◽  
К.В. ЗЕНКИНА ◽  
И.В. ЛОМАКИНА ◽  
З.С. РУБАН

Тенденция ухудшения качества зерна пшеницы, отмечающаяся в настоящее время, влечет за собой закономерное снижение хлебопекарных свойств муки. В связи с этим изучены особенности технологических и хлебопекарных свойств семи сортов яровой мягкой пшеницы селекции Дальневосточного НИИСХ – Хабаровчанка, Зарянка, Лира-98, Елизавета, Приамурская, Анфея, Далира. Оценку показателей качества зерна и хлеба проводили по общепринятым методикам ежегодно в 2006–2019 гг. Сорта яровой мягкой пшеницы Приамурская, Анфея, Далира в среднем за годы исследований отличались максимальным формированием урожая зерна (3,4; 3,8; 3,8 т/га соответственно). Натура зерна у сорта Далира соответствует I классу зерна мягкой пшеницы и составляет 756 г/л. Все изучаемые образцы яровой мягкой пшеницы имеют достаточно высокое содержание белка в зерне (более 14 %) и клейковины (более 28 %) второй группы качества (удовлетворительно слабая). Показатель «сила муки» существенно изменялся в зависимости от гидротермических условий вегетации (V = 6–37 %), в среднем за годы исследований максимальное значение данного признака (более 280 е.а.) установлено у сортов Лира-98, Елизавета, Приамурская, Далира. В благоприятные годы объемный выход хлеба составляет более 1000 мл у генотипов Зарянка, Лира-98, Елизавета, Анфея. Согласно пробной выпечке хлеба выделены сорта пшеницы с высокой хлебопекарной оценкой (выше 4 баллов) – Зарянка, Лира-98, Елизавета, Приамурская, Анфея, Далира. Таким образом, изученные образцы яровой мягкой пшеницы перспективны для использования в хлебопекарной промышленности. Currently, there is a tendency of deterioration in the quality of wheat grain, which entails a natural decrease in the baking properties of flour. In this regard, the technological and baking properties of 7 cultivars of spring soft wheat, bred in the Far Eastern Agricultural Research Institute, were studied. These cultivars were: Khabarovchanka, Zaryanka, Lira-98, Elizaveta, Priamurskaya, Anfeya, Dalira. The assessment of bread and grain quality was conducted according to the standard methodology in 2006–2019. Spring soft wheat cultivars Priamurskaya, Anfeya, Dalira on average differed in the maximum formation of grain yield over the years of study (3.4, 3.8, 3.8 tons per ha respectively). The grain nature of the Dalira cultivar was 756 g/l, which corresponds to the I class of soft wheat grain. All studied samples of spring soft wheat had a fairly high content of protein (more than 14 %), and gluten (more than 28 %) of the second quality group (satisfactory weak) in grain. The flour strength indicator significantly changed depending on the hydrothermal conditions of the growing season (V = 6–37 %), and on average, over the years of research, the maximum value of this trait (more than 280 e.a.) was found in cultivars Lira-98, Elizaveta, Priamurskaya, Dalira. In favourable years, the volume of bread from the Zaryanka, Lira-98, Elizaveta, Anfeya was more than 1,000 ml. According to the test bread baking, wheat cultivars with a high baking score (above 4 points) were highlighted: Zaryanka, Lira-98, Elizaveta, Priamurskaya, Anfeya, Dalira. Therefore, the studied cultivars of spring soft wheat are promising for use in the baking industry.


2014 ◽  
Vol 39 (1) ◽  
pp. 67-77
Author(s):  
Ataur Rahman ◽  
MM Rahman ◽  
MM Hasan ◽  
Farida Begum ◽  
MAZ Sarker

A field experiment was conducted at Bangladesh Agricultural Research Institute (BARI) farm, Gazipur to evaluate the effect of foliar application of potassium orthophosphate on grain yield and kernel quality of wheat under the terminal heat stress imposed by late sowing for two consecutive years (2008-09 and 2009-10). Five combinations of foliar applications of potassium orthophosphate and Tilt were tested on three wheat varieties, namely Kanchan, Shatabdi, and Prodip. The result indicated that foliar application of potassium orthophosphate was effective in increasing SPAD value (Measure of leaf chlorophyll content) and leaf area of all the wheat varieties, whereas Tilt application was effective only in Kanchan. The grain size of wheat in terms of 1000-grain weight was improved and thereby contributed to grain yield. Also the foliar application of potassium orthophosphate decreased the number of immature, smaller and deformed kernel, and thus improved the kernel quality. Two foliar sprays of 0.1% potassium orthophosphate solution at 70 DAS (Days after sowing) and 80 DAS performed better results than other applications. There were varietal differences in response to foliar treatments and the variety Prodip and Shatabdi were more responsive to potassium orthophosphate compared to Kanchan. Potassium orthophosphate could be suggested to improve grain yield and kernel quality of wheat under terminal heat stress condition. DOI: http://dx.doi.org/10.3329/bjar.v39i1.20144 Bangladesh J. Agril. Res. 39(1): 67-77, March 2014


Author(s):  
E.A. Gordeeva ◽  
N.A. Shestakova ◽  
R.G. Sakan

The practice of manufacturers using substances that stimulate the growth and development of plants, increase their resistance to external influences, and as a result increase the yield and quality of grain, has become a common practice in field cultivation technology. A wide variety of their production requires careful study and detailed application in agricultural practice. In the process of studying the variety of spring soft wheat after treatment with the studied organo-mineral mixtures (OMM) of the B-plus line, PRK «White Pearl» increases the vegetative mass and productivity, and improves the technological qualities of grain. The article presents data on the processing of seeds and plants of ОMM on the formation of the yield of varieties of spring soft wheat Astana and Karaganda 30. Studies have shown that the varietal reaction of soft wheat showed different responsiveness to treatment with drugs. Thus, grain yield was obtained from the use of the B-plus line of PRK «White Pearl» - Astana varieties 27.87 c/ ha, Karaganda varieties 30 - 32.16 c / ha (increase + 6.3; 8.75 c / ha in comparison with the control) with an increase in the amount of gluten by 0.1-2.2% and its quality.


2021 ◽  
pp. 15-21
Author(s):  
Raisa I. Belkina ◽  
Julia A. Letyago ◽  
Victor V. Vidrin ◽  
Tatyana K. Fedoruk

2020 ◽  
Vol 4 (24) ◽  
pp. 143-151
Author(s):  
S.V. Podgorny ◽  
◽  
O.V. Skripka ◽  
A.P. Samofalov ◽  
S.N. Gromova ◽  
...  

Improving the quality of wheat grain is an important task of agricultural production. In recent years, the production of strong and valuable wheat, necessary for the production of high-quality baking flour, has decreased. In this regard, the study of the quality of grain varieties of winter wheat in specific soil and climatic conditions of the Rostov region is becoming topical. Therefore, the purpose of our research was to study the main indicators of grain quality of varieties of soft winter wheat (protein and gluten content, flour strength, bread size from 100 g of flour) in ecological variety trials to select the best under conditions of the Rostov region. The studies were carried out on the trial fields of the Laboratory of selection and seed production of winter soft wheat of intensive type of the State Scientific Establishment “Agricultural research center «Donskoy»” (Rostov region) in 2014–2016. Seventy-five varieties were studied in the course of the research. Planting dates – optimal for implementation of agricultural and agro-technical measures. Planter – «Wintersteiger Plotseed S.» Seed placement depth – 4–6 cm. Preceding crop – black fallow. Accounting square of fields – 10 m2, double replication. Seeding rate – 4.5 million seeds per hectare. Grain quality was assessed according to the methods of the national standards of the Russian Federation. Such grain quality indicators as gluten content (according to GOST R 54478-2011), protein mass fraction (as required by GOST 108460-91), baking properties of flour (in a laboratory using the remix method) were determined. The article discusses the main indicators of the quality of grain and flour of winter soft wheat varieties in ecological variety testing and compares them. Analysis of the main indicators of the quality of varieties in trials showed that in the south of the Rostov region in 2014–2016 flour strength and gluten content were the most variable indicators. In terms of protein content (> 14.5 %), 8.0 % of the studied varieties were in full compliance with strong wheat standards; by the gluten content (> 28 %) – 7.0 %; by the flour strength (>280 e.a.) – 9.0 %. Five varieties of winter soft wheat: ‘Aksinya’, ‘Tanais’, ‘Asket’, ‘Nakhodka’, ‘Yumpa’ (Russia) with a complex of economically valuable traits are of greater interest for practical breeding in terms of improving grain quality.


2020 ◽  
Vol 9 (29) ◽  
pp. 124-136
Author(s):  
Anastasia Afonasyevna Kazak ◽  
Yury Pavlovich Loginov

Over the past century and at the beginning of the current one, Siberian breeders have been intensively breeding spring soft wheat for improving the yield and grain quality. The ecologically and geographically diverse source material has been used at the breeding and educational institutions of the region. Thus, in the severe Siberian conditions, the wheat gene pool has been painstakingly created. It has been very difficult here to solve the problem of improving the grain quality, but, given the determination and the talent of many breeders and geneticists, the gene pool of valuable and strong wheat varieties has been created. As a result, being formerly a consumer of food wheat, Siberia has now become a region producing high-quality grain for domestic use and sale in the foreign market. The gene pool of spring soft wheat, which is so rich in the volume and quality, has not been studied in full, and not used throughout Siberia. In 2013 – 2015, sixteen valuable and strong varieties of Siberia-bred wheat had been studied in the northern zone of the Tyumen region on the experimental field of the Northern Trans-Ural State Agricultural University. It has been found that the average productivity of the early-ripening Novosibirskaya 16, Tulun 15, and Polyushko varieties over the three years amounted to 2.52 – 2.89 t/ha, and was close to the yield (2.35 – 3.10 t/ha) of the middle-early ripening varieties. In the midseason-ripening group, the Alyoshina and Altayskaya 110 and 325 varieties exceeded the Tyumen-bred standard Lutescens 70 by 0.23 – 0.69 t/ha with the yield of the latter being 2.54 t/ha. In terms of the content and quality of gluten, the following varieties stood out: Iren, Novosibirskaya 15, Tulun 15, Polyushko, Omskaya 36, Skala, Boyevchanka, Pamyati Vavenkova, Tyumenskaya 80, Alyoshina, and Altayskaya 325. In terms of baking properties (3.7 – 4.2 points), the following varieties stood out: Iren, Novosibirskaya 15, 29, 31, Polyushko, Skala, Boyevchanka, Pamyati Vavenkova, Tyumenskaya 80, Tulunskaya 12, and Altayskaya 325, which can be used as the source material for further wheat breeding.


2020 ◽  
pp. 31-35
Author(s):  
O. A. Nekrasova ◽  
N. S. Kravchenko ◽  
N. G. Ignatieva ◽  
D. М. Marchenko ◽  
М. М. Ivanisov

2020 ◽  
pp. 26-31
Author(s):  
N. S. Kravchenko ◽  
N. E. Samofalova ◽  
I. M. Oldyreva ◽  
T. S. Makarova

The current paper has presented the study results of grain quality and pasta properties of the new and promising winter durum wheat varieties developed in the FSBSI “Agricultural Research Center “Donskoy” in 2016–2018. The purpose of the study was to evaluate winter durum wheat varieties according to such traits as grain quality and pasta properties to identify the best genotypes and recommend them for use in the breeding process and wider introduction into production. There have been identified the varieties “Yubilyarka” (96%), “Kristella” (94%), “Lakomka” (92%), “Diona” and “Yantarina” (91%) with maximum values of general hardness. All studied genotypes have formed the nature weight of grain corresponding to a first quality class (from 786–814 g/l). There have been identified the varieties “Kristella” (13.9%), “Donchanka” (13.8%), “Diona” (14.0%), “Yakhont” (14.0%), “Yubilyarka” (13.6%), “Yantarina” (13.7%), “Uslada” (13.6%), “Zoloto Dona” (13.6%), “Solntsedar” (13.9%) and “Almaz Dona” (13.5%) with protein percentage in grain corresponding to a first quality class. As a result of the study, it was found that all the studied varieties had good rheological properties of gluten and formed a DCI from 80 units (the variety “Eyrena”) to 102 units (the variety “Donchanka”) and belonged to the 2-nd group. The varieties “Diona”, “Eyrena”, “Yakhont”, “Yantarina” and “Solntsedar” had high rheological properties of dough. There have been identified the genotypes of the varieties “Donchanka” (613 μg/%), “Solntsedar” (626 μg/%) and “Almaz Dona” (630 μg/%) with the maximum concentration of carotenoid pigments, which could be used as sources to improve the trait “carotenoid content”. Throughout the years of study, the varieties were characterized by high indices of dry pasta color (4–5 points). According to a brittle strength of dry pasta there have been identified the varieties “Kristella” (775 g), “Donchanka” (774 g), “Diona” (757 g), “Yantarina” (782 g), “Solntsedar” (766 g) and “Dinas” (769 g) with the best indices of the trait. The varieties identified both by a single and by a set of traits characterizing grain quality of winter durum wheat and its final products, can be used in the breeding process as an adaptive initial material. The introduction of the best varieties into production will partly solve the problem of deficiency of high-quality durum wheat in the south of Russia.


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