scholarly journals Structural changes of duodenal mucosa enterocytes of rats in burn skin injury under experimental streptozotocin-induced diabetes mellitus

2019 ◽  
Vol 25 (1) ◽  
pp. 55-61 ◽  
Author(s):  
V.G. Cherkasov ◽  
I.O. Tymoshenko
Keyword(s):  
Diabetes Mellitus ◽  
Mucous Membrane ◽  
Structural Changes ◽  
Burn Injury ◽  
Duodenal Mucosa ◽  
The Body ◽  
Experimental Simulation ◽  
Skin Burn ◽  
Streptozotocin Induced Diabetes ◽  
Experimental Group

The work is devoted to the study of structural changes of enterocytes in the mucous membrane of the duodenum in a burn injury of the skin of a rat under conditions of experimental streptozotocin induced diabetes. The study was carried out on laboratory white adult rats-males weighing 180-210 g. The control group consisted of 21 animals without somatic pathology, the first experimental group consisted of 21 rats with skin burn injury, the second experimental group cleared 21 rats with skin burn and experimental streptozotocin induced diabetes. A model of experimental diabetes mellitus was reproduced by administering streptozotocin intraperitoneally once in dose of 50 mg/kg to rats. In an experimental simulation of a skin burn, two copper plates in the form of an ellipse were kept in water at 100oC for 10 minutes and, under ether anesthesia conditions, were applied simultaneously symmetrically on both exposed parts of the body of rats with an exposure for 10 seconds. Burn skin damage in rats was II-AB degrees – dermal surface burn (according to the old classification III-A degree) with a total area of 21-23% of the body surface with the development of burn shock. For morphological studies, the duodenum was taken, fragments of which were processed using conventional light and electron microscopy. The main criteria for assessing damage the enterocytes of the duodenal mucosa were the results studies of histological and ultrastructural data over 7, 14 and 21 days after a skin burn. The results of the studies showed that the damage of the enterocytes of the duodenal mucosa is based on deep destructive changes, which after 21 days (at the stage of septic toxemia), as a rule, are not reversible and develop against the background of significant intoxication of the organism. In the mucous membrane of the duodenum with burn injury of the skin associated with diabetes mellitus, there is a deterioration of the manifestations of the adaptive response and prolongation of destructive processes, accompanied by a violation of intercellular interactions in cytoarchitectically modified and deformed villi and crypts.

scholarly journals Structural changes of the intestinal epithelial barrier of the duodenum of rats in burn injury of skin under experimental streptozotocin-induced diabetes mellitus

2019 ◽  
Vol 25 (2) ◽  
pp. 28-35
Author(s):  
I.O. Tymoshenko
Keyword(s):  
Diabetes Mellitus ◽  
Structural Changes ◽  
Burn Injury ◽  
The Body ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Cytoplasmic Matrix ◽  
Streptozotocin Induced Diabetes ◽  
Experimental Group

The aim of the study was to study the structural changes of the intestinal epithelial barrier in the duodenum in burn injury of skin in rat under experimental streptozotocin-induced diabetes mellitus. The study was carried out on laboratory white adult rats-males weighing 180-210 g. The control group consisted of 21 animals without somatic pathology, the first experimental group was 21 rats with burn skin injury, the second experimental group was 21 rats with burn skin and experimental streptozotocin-induced diabetes mellitus. The experimental diabetes model was reproduced by administering streptozotocin to the rats intraperitoneally at a single dose of 50 mg/kg. Thermal burn skin damage in rats corresponded to II – A-B degrees of dermal surface burn (according to the old classification III – A degree) with a total area of 21-23 % of the body surface with the development of burn shock. Duodenum was selected for morphological studies, fragments of which were processed by conventional methods of light and electron microscopy. The main criteria for assessing damage to the duodenal mucosa enterocytes were the results of a study comparing histological and ultrastructural data in dynamics at 7, 14, and 21 days after skin burns. The results of the studies showed that the base of damage to the intestinal epithelial barrier of the duodenum are deep destructive changes, which after 21 days (in the stage of septicotoxemia), as a rule, are not reverse and develop on the background of significant intoxication of the body. There was a decrease in the number of tight junctions in the intestinal epithelial barrier of the duodenum of rats of the first and second experimental groups and a loss of ordering (acquisition of some chaotic nature) of their localization as the time after burn injury increased. For the most part, the intestinal epithelial barrier loses the integrity of the cellular component with partial preservation of the basement membrane (the first occurs both due to necrosis of the enterocytes with a brush border and due to complete destruction of goblet cells). In all cases, defects exist in the intestinal epithelial barrier, which are potential pathways for paracellular translocation of the pathogenic contents of the duodenum. It is not inconceivable that part of this intestinal pathogenic content can be translocated also by partially damaged cells. Evidence of the latter is the presence of microbial bodies in the cytoplasm of cells with partially destroyed plasmalemma (but preserved organelles and nucleus). An adaptive mechanism for ensuring the repair of damaged enterocytes is selective autophagy, which acts as a factor in the recycling of destroyed organelles and the cytoplasmic matrix, aimed primarily at maintaining cell viability.

scholarly journals STRUCTURAL CHARACTERISTICS OF THE RAT DUODENAL MUCOSA DURING BURN INJURY TO THE SKIN UNDER THE CONDITIONS OF EXPERIMENTAL STREPTOZOTOCININ-INDUCED DIABETES MELLITUS

2019 ◽  
pp. 35-47
Author(s):  
Irina Tymoshenko
Keyword(s):  
Diabetes Mellitus ◽  
Er Stress ◽  
Burn Injury ◽  
Duodenal Mucosa ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Skin Burn ◽  
Streptozotocin Induced Diabetes ◽  
Experimental Group

The work is devoted to the study of structural features of the mucous membrane of the duodenum in case of burn injury to the skin under experimental streptozotocin-induced diabetes mellitus. The study was carried out on laboratory adult white and male rats weighing 180-210 g. The control group consisted of 21 intact animals, and the I experimental group consisted of 21 rats with experimental skin burn injury, experimental II the group consisted of 21 rats without skin burns with experimental streptozotocin-induced diabetes, the III experimental group consisted of 21 rats with skin burn and experimental streptozotocin induced diabetes. The experimental diabetes mellitus model was reproduced by administering rats streptozotocin intradermally once in a dose of 50 mg / kg. Thermal burn skin damage in rats corresponded to II - AB degree - dermal superficial burn (according to the old classification III - A degree) with a total area of ​​21-23% of the body surface with the development of burn shock. For morphological studies, a section of the duodenum was taken, fragments of which were processed by conventional methods of light and electron microscopy. The main criteria for assessing damage of the duodenal mucosa were the results of a study comparing histological and ultrastructural data in dynamics after 7, 14 and 21 days after a skin burn. The results of the studies showed a decrease in the number of tight contacts in the intestinal epithelial barrier of the mucous membrane of the duodenum of rats of the first and third experimental groups and the loss of order (the acquisition of a certain randomness) of their localization with increasing time after a burn injury. Defects appear in the intestinal epithelial barrier, which are potential pathways of paracelular translocation of the pathogenic contents of the duodenum. It is possible that part of this intestinal pathogenic content can be translocated and through partially damaged cells. Evidence of the latter is the presence of microbial bodies in the cytoplasm of cells with partially destroyed plasmolemma (but preserved organelles and nucleus). The adaptive mechanism for ensuring the repair of damaged enterocytes is selective autophagy, which acts as a factor in the recycling of the destroyed organelles and cytoplasmic matrix material, primarily aimed at maintaining cell viability. The initiation of autophagy associated with the development of enterocytes stress endoplasmic reticulum (ER-stress). In the enterocytes of the duodenum of rats of the third experimental group, ER-stress occurs so quickly and is accompanied by a steady spread of destruction processes in the cell, autophagy mechanisms do not have time to work, and most cells die. Thus, the absence of an autophagous reaction is a marker of the unfavorable direction of the course of post-burn structural changes in enterocytes.

scholarly journals Comparative characteristics of the manifestations of damage and reparative processes in the mucous membrane of the duodenum of rats under the conditions of skin burns and skin burns associated with diabetes

2020 ◽  
Vol 26 (2) ◽  
pp. 39-44
Author(s):  
I.A. Tymoshenko ◽  
L.M. Sokurenko ◽  
A.Ya. Yanchyshyn ◽  
V.A. Pastukhova
Keyword(s):  
Diabetes Mellitus ◽  
Mucous Membrane ◽  
Structural Changes ◽  
Burn Injury ◽  
Experimental Diabetes ◽  
Male Rats ◽  
Control Group ◽  
Experimental Diabetes Mellitus ◽  
Buffer Solution ◽  
Solution Ph

Currently, severe thermal injury is becoming one of the most important problems of practical medicine. Diabetes is also recognized as another global medical and social challenge of our century. The emergency situation for the treatment and prevention of the consequences of these pathologies is a consequence of the lack of a reliable theoretical basis for solving specific clinical problems regarding the course of burns, diabetes and their complications. The aim of the study is to establish the patterns of structural changes in the mucous membrane of the duodenum after burn injury of the skin of rats under conditions of experimental diabetes mellitus. The study was performed on 63 laboratory white adult male rats weighing 180-210 g, which were divided into 3 groups: intact animals, rats with skin burns and rats with skin burns on the background of diabetes. The model of experimental diabetes mellitus was reproduced by administering Streptozotocin to rats intraperitoneally once at a dose of 50 mg/kg, pre-dissolved in 0.1 M citrate buffer solution (pH=4.5). The control of the development of hyperglycemia in the experimental groups was the level of glucose in the blood 24.24±0.79 mmol/l. In the control group this index was 8.03±0.4 mmol/l. Rats with skin burns revealed destructive manifestations, which are accompanied by an active inflammatory reaction and corresponding necrotic changes, while rats with skin burns on the background of diabetes mellitus pathological processes are not just “summed up”, but in some way adaptively modified with the involvement of stress mechanisms of the endoplasmic reticulum and associated autophagy.

scholarly journals Effects of armadin on hepatic transaminases in rats with acute generalized peritonitis on the background of diabetes mellitus

2021 ◽  
Vol 11 (5) ◽  
pp. 333-338
Author(s):  
M. Regeda ◽  
B. Verveha ◽  
N. Melnyk
Keyword(s):  
Diabetes Mellitus ◽  
Abdominal Cavity ◽  
White Male ◽  
The Body ◽  
Laboratory Animals ◽  
Control Group ◽  
Male Adult ◽  
Generalized Peritonitis ◽  
Streptozotocin Induced Diabetes ◽  
Experimental Group

The article discusses the issues about the effect of the Armadin on the hepatic transaminase in rats with acute generalized peritonitis on the background of diabetes mellitus. The experiment was performed on 48 white male adult rats, which were divided into two groups. The experimental group – animals with simulated acute generalized peritonitis against the background of streptozotocin-induced diabetes with the drug “Armadin” (ethylmethylhydroxypyridine succinate) application (n=24). The control group – animals with simulated acute generalized peritonitis against the background of streptozotocin-induced diabetes without pharmacocorrection (n = 24). Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin at the dose of 60 mg/kg. Acute generalized peritonitis was induced with 10% faecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method. Every day for 6 days, rats of experimental group received the drug “Armadin” at the doses of 100 mg/kg. We found a probable decrease in aminotransferase activity on day 3 of the study in rats of the experimental group after administration of Armadin. Thus, ALT activity decreased by 26.7%, and AST activity – by 25.9% compared to the control group. On the 7th day of the study, the activity of ALT in the blood of rats of the experimental group decreased to 0.50 ± 0.03 mmol/l, which was 49.5% lower than the control group of rats in this period of the experiment. Similar changes are observed in the study of AST activity, where, accordingly, it decreased by 48 % relative to control. The positive effect of the drug "Armadin" on the body of rats, with AGP on the background of STZ-induced diabetes, is manifested by the restoration of the functional state of the liver.

scholarly journals Morphological Characteristics of Changes in the Duodenal Wall Within 14-56 Days of the Development of Streptozotocin-Induced Experimental Diabetes Mellitus

2020 ◽  
Vol 27 (4) ◽  
pp. E2020413
Author(s):  
Ihor Bilinskyi
Keyword(s):  
Diabetes Mellitus ◽  
Lamina Propria ◽  
Experimental Diabetes ◽  
Histological Study ◽  
Duodenal Mucosa ◽  
Male Rats ◽  
Duodenal Wall ◽  
Apical Surface ◽  
Experimental Diabetes Mellitus ◽  
Streptozotocin Induced Diabetes

The objective of the research was to determine the morphological features of the duodenal wall of animals within 14-56 days of developing streptozotocin-induced diabetes mellitus using light optical microscopy. Materials and Methods. The research was carried out on 40 white nonlinear adult male rats. Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin (Sigma, USA) at a dose of 60 mg/kg body weight. The material was taken from the duodenum on the 14th, 28th and the 56th days after the onset of experimental diabetes mellitus. For histological study, the preparations were made using the conventional method, which included the staining of sections with hematoxylin and eosin. Results. Streptozotocin-induced diabetes mellitus was experimentally found to lead to dystrophic changes in the epithelial components of the duodenal mucosa from the 14th day of developing. There were observed a shortening of the villi of the mucous membrane and a lack of distinctness of striated border contours on the apical surface of epitheliocytes. Between the connective-tissue fibers of the lamina propria of the mucosa and thin-walled vessels, the cellular elements, including mainly macrophages, lymphocytes, were found. There was a shortening of the villi, edema and histiolymphocytic infiltration of the villous stroma 28 days after developing experimental diabetes mellitus. The epithelium covering was discontinuous; numerous areas of desquamation were found at the apex of the villi. Fifty-six days after developing experimental diabetes mellitus, the destruction and desquamation of the epithelium of the villi and crypts were observed. The surface of the duodenal mucosa smoothed down due to the shortening and flattening of the villi (indicating their atrophy), while the crypts elongated and their depth increased. Conclusions. Histological study of the duodenal wall of diabetic animals showed pronounced desquamation at the apex of the villi, destructive and dystrophic changes in the surface epithelium, edema and increased cellular infiltration of the lamina propria of the mucosa. Thus, in diabetes mellitus, structural changes in the duodenal wall of rats are characterized by the dystrophic processes, which can be considered as the morphological reflection of enteropathy.

scholarly journals Morphological features of the large intestine in experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes

2021 ◽  
pp. 41-45
Author(s):  
B. M. Verveha
Keyword(s):  
Diabetes Mellitus ◽  
Mucous Membrane ◽  
Abdominal Cavity ◽  
Morphological Features ◽  
Male Rats ◽  
The Third ◽  
Acute Peritonitis ◽  
Perivascular Edema ◽  
Fecal Suspension ◽  
Streptozotocin Induced Diabetes

The work is a fragment of the research project “Pathogenetic features of the allergic and inflammatory processes course and their pharmacocorrection”, conducted by the Department of Pathological Physiology, Danylo Halytskyi Lviv National Medical University (state registration No. 0116U004503). The aim of the work: to study the morphological features of the colon in the dynamics of the development of experimental acute peritonitis on the background of streptozotocin-induced diabetes mellitus. Materials and Methods. The experiment used 48 white male rats. Diabetes mellitus in experimental animals was simulated by a single intraperitoneal injection of streptozotocin (Sigma) at a rate of 60 mg/kg, acute disseminated peritonitis – the introduction of 0.5 ml of 10 % filtered fecal suspension into the abdominal cavity. A morphological study of the colon in animals removed from the experiment on the first, third and seventh days of acute peritonitis on the background of concomitant diabetes mellitus was performed. Results and Discussion. Morphological examination of the colon of animals on the first day of experimental acute peritonitis in conditions of concomitant streptozotocin-induced diabetes mellitus revealed an increase in the size of the crypts due to stroma edema and lymphohistiocytic infiltration, slight perivascular edema in the subclavian edema. On the third day, thickening of the mucous membrane of the colon, a sharp increase in the depth of the crypts, uneven blood supply to the vessels in the submucosal layer with a predominance of perivascular edema were verified. On the seventh day, a decrease in the thickness of the mucous membrane due to the expansion of the crypts was visualized. Part of the epitheliocytes was in a phase of increased secretory activity, the other part was dystrophically altered, which stimulated increased lymphocytic and histiocytic infiltration. These changes were accompanied by activation of reactive processes and hyperplasia of lymphoid follicles on the first day of peritonitis on the background of streptozotocin-induced diabetes mellitus, and as the severity of purulent inflammation – hypoplasia of the lymphoid tissue and a decrease in local reactivity(the third and seventh days of the development of acute peritoneal burning in conditions of combined pathology).

scholarly journals Structural changes of mucous membrane and the presence of immunoglobulins in small intestines of neonatal pigs

2013 ◽  
Vol 82 (3) ◽  
pp. 317-322
Author(s):  
Tomáš Kanka ◽  
Michal Rolinec ◽  
Danka Šťastná ◽  
Ivan Imrich ◽  
Daniel Bíro ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Structural Changes ◽  
Postnatal Period ◽  
Control Group ◽  
Large White ◽  
Neonatal Pigs ◽  
Large White Breed ◽  
White Breed ◽  
Mucosal Changes ◽  
Experimental Group

The structure of the small intestine of neonatal pigs has an important effect on immunoglobulin absorption. The aim of the experiment was to observe changes in the mucous membrane and the presence of immunoglobulins in the intestines of piglets during the first hours of their postnatal lives. In total, 80 piglets of the Large White breed were divided into two groups of 40 pigs. One group (control) came from an infection-free breeding, the other (experimental) group came from a breeding with the reproduction and respiration syndrome of pigs. Samples (n = 80) of duodenum, jejunum and ileum were taken at intervals of 0, 3, 6 and 12 h after colostrum intake in order to carry out histological tests. Primary pig antibodies were detected by immunohistochemical staining. The low content of immunoglobulins IgG, IgA and IgM was detected in all segments of intestines of precolostral piglets. Difference (P < 0.05) in size of the mucous membrane was found in the duodenum at the interval of 0, 3 and 6 h (46.03%), in the jejunum at 0 and 12 h (45.98%) and in favour of the control group. Compared to the control group, more Ig (P < 0.01) antibodies were observed in the experimental group: in the duodenum IgG at 12 h (37.22 vs. 10.81%), IgA, 6 h (43.29 vs. 16.71%), IgM, 12 h (25.29 vs. 8.40%), in the jejunum at IgG 12 h (23.97 vs. 9.96%), IgA, 0 h (0.48 vs. 0.37%) and IgM, 3 h (21.80 vs. 10.10%), in the ileum IgG, 0 h (0.38 vs. 0.29%), IgA, 3 h (20.46 vs. 6.45%), IgM, 3 h (31.5 vs. 12.29%). We can conclude that the colostrum intake and the presence of reproduction and respiration syndrome of pigs resulted in changes of microscopic structures of intestines as well as in the presence of Ig in piglet intestines. These mucosal changes significantly affect the development of passive immunity in piglets in the postnatal period.

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