scholarly journals Investigation of the combined use effect of the antiseptic decamethoxin and fluoroquinolones on clinical strains S. aureus

2020 ◽  
Vol 24 (1) ◽  
pp. 80-83
Author(s):  
O.A. Nazarchuk ◽  
S.V. Pavliuk ◽  
H.H. Nazarchuk ◽  
V.M. Mruh ◽  
A.O. Dudar ◽  
...  
Keyword(s):  
Antimicrobial Properties ◽  
High Sensitivity ◽  
Infectious Complications ◽  
Chemotherapeutic Agents ◽  
Staphylococcal Infection ◽  
Dilution Method ◽  
Antibiotic Resistant ◽  
Clinical Strains ◽  
Resistant Strains ◽  
Low Sensitivity

Annotation. Postoperative infectious complications in eye microsurgery are most often caused by S. aureus strains, among which resistance to fluoroquinolones, first-line drugs for the prevention of postoperative complications, is common. An alternative to fluoroquinolones is antiseptics. The aim of the study was to investigate the effect of decamethoxin antiseptic on the antimicrobial properties of fluoroquinolones against S. aureus. Studies on the combined effect of the antimicrobial properties of fluoroquinolones and decamethoxin (DCM) were performed on the S. aureus museum strain ATCC 25923, on moderately stable and persistent S. aureus clinical strains (n=42) obtained from patients undergoing eye microsurgical procedures, by serial dilution method. The minimum bacteriostatic concentration (MBsC), the minimum bactericidal concentration (MBcC) of antimicrobials separately in pure form and with the addition of sub-bacteriostatic concentrations (subBsC, 1/4 MBsC) of DCM were determined. Statistical data processing was performed using special and office programs “STATISTICA 6.0”, “Microsoft Excel 2010”. The study found low sensitivity to fluoroquinolones in clinical strains of S. aureus, high sensitivity to DCM (MBsC 0.66±0.1; MBcC 3.19±0.4 μg/ml). The sensitivity of resistant and moderately resistant strains of S. aureus to fluoroquinolones in the presence of subBsC DCM was established: MBsC of ciprofloxacin in the presence of DCM decreased almost 4 times, norfloxacin — 5.5 times, ofloxacin — 6.8 times, of levofloxacin — 6.8 times, moxifloxacin 7.1 times. It was found that MBcC of norfloxacin for clinical resistant S. aureus strains decreased in 5.7 times, ofloxacin — in 9.2 times, levofloxacin — in 6.9 times, ciprofloxacin — in 8.6 times, moxifloxacin — in 7.9 times. The simultaneous use of antiseptic DCM and various fluoroquinolone chemotherapeutic agents provides effective protection against staphylococcal infection, contributes to the fight against antibiotic resistant strains of S. aureus.

scholarly journals Effect of Naseptin cream prophylaxis on staphylococcal infection in adult surgical wards and infant nurseries

1962 ◽  
Vol 60 (2) ◽  
pp. 209-215 ◽  
Author(s):  
E. Joan Stokes ◽  
Shelagh E. Milne
Keyword(s):  
Wound Infection ◽  
Marked Effect ◽  
Controlled Trial ◽  
Nasal Carriage ◽  
Staphylococcal Infection ◽  
Antibiotic Resistant ◽  
Resistant Strains ◽  
Made In

A controlled trial of Naseptin cream, as an anti-staphylococcaiprophylactic, made in two adult wards, showed the cream to be effective in reducing nasal carriage, including antibiotic resistant strains. There was no marked effect on wound infection.A controlled trial of Naseptin cream and Sterzac powder prophylactically in maternity nurseries was found to be effective in reducing nasal carriage and infection.These findings are discussed, and recommendations on the use of Naseptin cream as a prophylactic are made.

scholarly journals Antibiotic resistance in Acinetobacter baumannii as agent of surgery infection and approaches to its overcoming by means of deca-methoxinum antiseptic

2019 ◽  
Vol 1 (2) ◽  
pp. 18-22
Author(s):  
O A Nazarchuk ◽  
V I Nahaichuk
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Sensitivity ◽  
Antibiotic Resistant ◽  
Clinical Strains ◽  
Minimal Inhibitory Concentrations ◽  
Hospital Acquired Infections ◽  
Causative Agents ◽  
Resistant Strains ◽  
Burn Disease ◽  
Hospital Acquired

Introduction. Non-fermenting Gram-negative bacilli are known as one of the most frequent causative agents of hospital-acquired infections. Acinetobacter baumannii, as causative agent of infection complications of different localization, has obtained recently high resistance to anti-biotics and has belonged to ESKAPE group of pathogens. Antimicrobials, recommended for the prophylaxis and therapy of hospital-acquired infections, have been failing in their effectiveness and lead to selection of antibiotic resistant strains of A. baumannii. The aim of this research was to substantiate the way of overcoming of resistance in clinical strains of A. baumannii, by means of synergic antimicrobial activity of antibiotics and antiseptic decamethoxinum®. Material and methods. The research was carried out on 190 clinical strains of A. baumannii, isolated from patients with burn disease during the period 2011–2015. The sensitivity of clinical strains of A. baumannii was determined to such antibiotics as ampicillin/sulbactam, cefoperazone, cefoperazone/sulbactam, meropenem, imipenem, amikacin, ciprofloxacin, gatifloxacin and antiseptic decamethoxinum® (DCM; Registration certificate No UA/14444/01/01 since 24.06.2015. Order of the Ministry of Health of Ukraine No 373). The sensitivity of A. baumannii to antibiotics and DCM was determined by means of disk diffusion test and serial dilution (Order of the Ministry of Health of Ukraine No167 since 05.04.2007; EUCAST expert rules).The study of the influence of antiseptic DCM on the sensitivity of acinetobacteria to antibiotics was studied on 35 clinical strains of A. baumannii, drafted from the general number of isolates enrolled in the research. For this, the sensitivity of A. baumannii to antibiotics in the presence of sub-minimal inhibitory concentrations (subMIC) of DCM was identified. The received experimental data were analyzed by “Statistica 6.0”. Results and discussion. The changes of antibiotic sensitivity profile of A. baumannii for five years were shown. It was found that the sensitivity of A. baumannii to majority of antibiotics, selected for study, decreased significantly. But the only ampicillin/sulbactam was found to have vice versa tendency. We found the rising quantity of antibiotic resistant strains of A. baumannii. At the same time, high resistance of acinetobacteria to fluoroquinolones (ciprofloxacin– 96,1%; gatifloxacin– 95,8%) was found in 2015. The in vitro research of combined activity of DCM antiseptic remedy and early mentioned antibiotics against clinical strains of A. baumannii demonstrated the reveal antibiotic effectiveness. As follows, minimal inhibitory concentrations of antibiotics decreased in 1.5–4 times in the mediums which contained subMIC of DCM. Especially this tendency was found in resistant clinical strains. Conclusion. Under selective influence of antibiotics protected by β-lactamase inhibitors, carbapenems, fluoroquinolones aminoglycosides increase the antibiotic resistance in A. baumannii, causative agents of infectious complications in patients with burn disease. The antiseptic remedy decamethoxinum® helps to improve antibiotic sensitivity in resistant A. baumannii.

scholarly journals Antimicrobial activity of antiseptics in the prevention of postoperative infectious complications

2021 ◽  
pp. 33-36
Author(s):  
N. A. Bagnyuk ◽  
O. A. Nazarchuk ◽  
Y. M. Babina ◽  
R. M. Chornopyshchuk ◽  
A. V. Kulyk
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Bactericidal Effect ◽  
Infectious Complications ◽  
Antimicrobial Efficacy ◽  
Gram Positive ◽  
Gram Negative ◽  
Clinical Strains ◽  
Enterococcus Spp

Recently, among hospital strains of microorganisms, an increase in the number of antiseptic-resistant strains of opportunistic pathogens has been registered, which significantly affects the effectiveness of these drugs. It is important to study their antimicrobial efficacy to justify rational use. The aim is to conduct a comparative study of the antimicrobial efficacy of antiseptics of decamethoxine, chlorhexidine, polyhexanide. During study we examined the antimicrobial activity against 186 clinical strains of microorganisms (Acinetobacter baumannii, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus spp., Enterobacter spp.) isolated from patients with infectious complications in the postoperative period. The minimum inhibitory (MIC) and bactericidal concentrations (MBC) of 0.02 % and 0.1 % decamethoxine, 0.05 % chlorhexidine bigluconate, 0.1 % polyhexanide were determined; antimicrobial efficacy of drugs was evaluated by the index of antiseptic activity by conventional methods. The study found high antimicrobial properties of decamethoxine, chlorhexidine, which had a high bactericidal effect on clinical strains of S. aureus, Enterococcus spp., Enterobacter spp. Proved the benefits of antimicrobial activity of the drug based on decamethoxine (p<0.001). The polyhexanide has pronounced antimicrobial properties against A. baumannii, bacteria of the family Enterobactericae, P. aeruginosa. Thus, the leading gram-positive (Staphylococcus aureus, enterococci) and gram-negative pathogens (enterobacteria, acinetobacteria, pseudomonads) are sensitive to polyhexanide, chlorhexidine and the domestic drug decamethoxin, with a probable advantage of the antimicrobial properties of the latter over all gram-positive and most gram-negative microorganisms.

The analysis of sensitivity of Escherichia clinical strains, isolated from ill children to antibiotics, antiseptics

2013 ◽  
Vol 4 (4) ◽  
pp. 23-27
Author(s):  
Gordiy Kondratyevich Paliy ◽  
Oleksandr Adamovych Nazarchuk ◽  
Dmitri Vladimirovich Paliy ◽  
Sergey Adamovych Nazarchuk ◽  
Oksana Olegovna Gonchar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
High Sensitivity ◽  
High Antimicrobial Activity ◽  
Clinical Strains ◽  
E Coli ◽  
Amoxicillin Clavulanate ◽  
Low Sensitivity

In the research the results of the study of sensitivity of Escherichia clinical strains (E. coli n 110) to antibiotics, antiseptics are presented. The Escherichia were isolated from ill children. According to the data of the research, we found high sensitivity of E. coli to combined penicillin antibiotics (amoxicillin/clavulanate, piperacyllin/tazobactam), cefalosporines (ceftriaxone, cefepime); meropenem; fluoroquilones (gatifloxacine, levofloxacine). The Escherichia had low sensitivity to amoxicillin/sulbactam, streptomycine, kanamycine, cefazoline, cefamandol, cefuroxime. High antimicrobial activity of decasan, miramistin with superiority of decasan according to E. coli, was proven.

DEPENDENCE OF BIOFILM-FORMING ABILITY ON THE ANTIBIOTIC SENSITIVITY OF PSEUDOMONAS AERUGINOSA CLINICAL STRAINS ISOLATED FROM PATIENTS WITH CHRONIC OSTEOMYELITIS

2020 ◽  
Vol 65 (1) ◽  
pp. 37-41
Author(s):  
I. V. Shipitsyna ◽  
E. V. Osipova ◽  
E. N. Ovchinnikov ◽  
D. S. Leonchuk
Keyword(s):  
Biofilm Formation ◽  
Chronic Osteomyelitis ◽  
Antibiotic Sensitivity ◽  
Antibiotic Resistant ◽  
Clinical Strains ◽  
Wide Range ◽  
Adhesive Ability ◽  
Resistant Strains ◽  
Clinically Significant ◽  
Tubular Bones

Due to the spreading highly resistant strains among clinically significant P. aeruginosa clones, it becomes necessary to prescribe antibiotics not only taking into account the knowledge of sensitivity spectrum of a particular isolate but the data of microorganism biofilm activity as well. To study the dependence of biofilm-forming ability on the sensitivity to antibacterial preparations of P. aeruginosa clinical strains, isolated from patients with chronic osteomyelitis. 36 patients above 18 with chronic osteomyelitis of long tubular bones who were treated in the center of purulent osteology took part in the experiment. Object of the study - material isolated from wounds, fistulas, as well as from inflammatory foci. The sensitivity of isolated microorganisms to 10 antibiotics was analyzed: Piperacillin/Tazobactam, Imipenem, Meropenem, Aztreonam, Amikacin, Ciprofloxacin, Ceftriaxone, Ceftazidime, Cefotaxime, Cefepime. High- and medium-adhesive strains accounted for 86,1 % among P. aeruginosa clinical isolates, obtained from the wounds of patients with chronic osteomyelitis of long tubular bones. Highly adhesive strains are resistant to a wide range of antibacterial preparations used clinically. Penicillins were the most effective preparations when analyzing antibioticograms obtained for highly adhesive strains, for medium adhesive strains - penicillins, aminoglycosides and carbapenems, for low adhesive ones - aminoglycosides, penicillins, carbapenems, monobactams, quinolones. P. aeruginosa multi-resistance is a serious problem in the treatment of patients with chronic osteomyelitis. Spreading antibiotic-resistant strains of P. aeruginosa is associated with the presence of bacteria in the biofilm. Since adhesion is the first step in the biofilm formation, it is important to identify strains having high adhesive ability timely.

scholarly journals Research of antimicrobial efficacy of modern antiseptic agents based on decamethoxine and povidone-iodine

2019 ◽  
Vol 2 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Oleksandr Nazarchuk
Keyword(s):  
Antimicrobial Activity ◽  
Povidone Iodine ◽  
Antimicrobial Properties ◽  
Infectious Complications ◽  
Antimicrobial Efficacy ◽  
Gram Positive ◽  
Gram Negative ◽  
Clinical Strains ◽  
E Coli ◽  
Enterococcus Spp

Under conditions of wide increased resistance of pathogens of infectious complications to antimicrobial agents, a considerable attention is paid to the use of antiseptic drugs. The research of their antimicrobial efficacy remains valid for substantiation of the rational use. The purpose was to carry out a comparative study of the antimicrobial efficacy of medicinal antiseptic agents based on decamethoxine and povidone-iodine. In the study there was examined antimicrobial activity of mentioned antiseptics against 682 clinical strains of microorganisms (A. baumannii, S. aureus, P. aeruginosa, Enterococcus spp., E. coli, Enterobacter spp., K. pneumoniae, Proteus spp.), isolated from patients with infectious complications. Minimum inhibitory and bactericidal concentrations of 1–2– 10 % iodine, 0.02 % decamethoxine (decasan) were determined. Antimicrobial efficacy of medicines, based on studied antiseptics was evaluated with the use of an index of antiseptic activity, calculated by means of commonly used methods. The study revealed high bactericidal properties of decasan against clinical strains of S. aureus, Enterococcus spp., E. coli, K. pneumoniae and Enterobacter spp. The advantages of antimicrobial activity of decamethoxine-based antseptis (decasan, p < 0.001) were proved. Iodine has expressed antimicrobial properties against Enterococcus spp., S. aureus, A. baumannii, bacteria of the Enterobacteriаcеae family and P. aeruginosa. It is proven that the dissolution of povidone-iodine leads to the reduction of the antimicrobial efficacy of 2 % antiseptic solution. There was found inefficacy of 1 % povidone-iodine against infectious agents (p < 0.001). Thus, the leading Gram-positive (S. aureus, Enterococci) and Gram-negative pathogens (Escherichia, Klebsiella, Enterobacteria, Acinetobacteria, Pseudomonas) have a sensitivity to iodine and to domestic preparation based on decamethoxin 0.02 % (decasan), with a definite advantage of the antimicrobial properties of the latter to Gram-positive and some Gram-negative microorganisms (p < 0.001).

scholarly journals Assessment of Antimicrobial Activities and Toxicological Effects of Green and Red Cultivars of Roselle- Hibiscus sabdariffa L

2020 ◽  
pp. 11-22
Author(s):  
Serifat Olatundun Salami ◽  
Anthony Jide Afolayan
Keyword(s):  
Brine Shrimp ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Artemia Salina ◽  
New Drugs ◽  
Dilution Method ◽  
Toxicological Effects ◽  
Resistant Strains ◽  
Antibacterial And Antifungal ◽  
Drug Resistant Strains

Aims: The use of synthetic antibiotics has been the major way of curing diseases; however, over-use of antibiotics has led to emergence of multi-drug-resistant strains of several groups of microorganisms. This study aimed at examining roselle extracts for antimicrobial properties with a view to providing the best alternative to the injudicious use of synthetic antibiotics and also examines the toxicological effects of roselle extracts. Methodology: Ethanolic and aqueous extracts of roselle leaves and calyces were evaluated for antimicrobial activity based on minimum inhibitory concentration (MIC50) using Broth dilution method. The toxicological effects based on LC50 were also evaluated using Brine shrimp- Artemia salina. Simple percentage was used to determine the mortality rate of the nauplii while the minimum inhibitory concentrations of the extracts were determined using MINITAB 17 statistical package (P<0.05). Data were expressed as mean ± standard deviation of three replicates. Results: Roselle calyces, pre-flowering green and flowering red exhibited excellent inhibition to bacteria. Calyces had better antimicrobial activities and higher toxicological effects than the leaves. Green roselle at pre-flowering stage and red flowering were good for antimicrobial screening. Conclusion: Roselle extracts possessed excellent antibacterial and antifungal properties. These can be explored to develop new drugs, which can suppress the resistant strains. All the plant extracts were greater than 1000 mg/mL, which indicates that they were non-toxic to brine shrimp larvae.

scholarly journals The Effect of Local Antibiotic on Nasal Carriage of Staphylococcus Pyogenes

1955 ◽  
Vol 53 (3) ◽  
pp. 379-385 ◽  
Author(s):  
J. C. Gould
Keyword(s):  
Nasal Carriage ◽  
Staphylococcal Infection ◽  
Control Group ◽  
Resistant Organism ◽  
Antibiotic Resistant ◽  
Resistant Strains ◽  
Local Antibiotic ◽  
Cessation Of Treatment

SummaryLanoline-water cream containing 1% of antibiotic was applied for 7–14 days to the anterior nares of 124 carriers of antibiotic sensitive staphylococci. Nose swab cultures, examined during the administration of the cream, were negative for Staph. pyogenes in all carriers who received oxytetracycline, and in 80, 75, 70 and 67% of those who received penicillin, chlortetracycline, streptomycin and chloramphenicol respectively.Seventy per cent of carriers gave nose-swab cultures negative for at least 2 weeks, 50% for at least 4 weeks and 25% for at least 20 weeks after the cessation of treatment. Over the same period only 1% of a control group ceased to be carriers.The nares of 20% of carriers receiving antibiotic were temporarily colonized with antibiotic-resistant strains, but in only one case (0·8%) did the resistant organism persist throughout the period of examination.The use of this method of controlling staphylococcal carriage in preventing staphylococcal infection is discussed.I wish to thank Prof. T. J. Mackie for his advice.

PROFILE OF COLIFORM STRAINS’ ANTIBIOTIC RESISTANCE

2019 ◽  
Vol 49 ◽  
pp. 135-157
Author(s):  
Chikviladze D. ჩიკვილაძე დ. ◽  
Gachechiladze Kh. გაჩეჩილაძე ხ. ◽  
Mikeladze M. მიქელაძე მ. ◽  
მეტრეველი დ. Metreveli D.
Keyword(s):  
Drug Resistance ◽  
Clinical Course ◽  
High Sensitivity ◽  
Multidrug Resistant ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Intestinal Infection ◽  
E Coli ◽  
Agar Dilution ◽  
Resistant Strains

  The spectrum of sensitivity of 112 E. coli strains isolated from patients with acute intestinal infection to 23 antibiotics was investigated  by the agar dilution method, which revealed an increase in drug resistance and the emergence of multidrug-resistant strains  (33.04%). It was found that the drug resistance of the pathogen to nalidixic acid and carbopenems increased and its high sensitivity to some ftuoroquinolones, III and IV generation aminoglycosides, penicillins and cephalosporins preserved. The drug resistance in future may lead to the formation of hospital strains among E. coli and alter an epidemiological process and the clinical course of the disease.

Effect of Bacteriostats on Cells Growth and Contamination-Resistant Capability of Rhizobium Inoculants

2011 ◽  
Vol 291-294 ◽  
pp. 2460-2465
Author(s):  
Jian Feng Li ◽  
Ping Hui Huo ◽  
Shang Li Shi ◽  
Shu Qing Zhang ◽  
Li Yu Chen
Keyword(s):  
Survival Rate ◽  
Doubling Time ◽  
Agar Plate ◽  
Relative Survival ◽  
Dilution Method ◽  
Nodule Occupancy ◽  
Antibiotic Resistant ◽  
Relative Survival Rate ◽  
Nodulation Competitiveness ◽  
Resistant Strains

The purpose of this study was to evaluate the effect of ampicillin as bacteriostats on the doubling time and relative survival rate of antibiotic resistantRhizobiumstrains and the ratio of undesirable microbes in artificially polluted inoculants during 60 days storage, and the nodule occupancy of two bacteriostat-resistant strains were also investigated. Fully grown Yeast-mannitol liquid media culture ofRhizobiummeliloti LW107 andRhizobiumsp. RSW 96 were added with ampicillin as bacteriostats at various concentrations in an artificial pollution test, and the relative survival rate of rhizobia and the counts of undesirable microbes were determined by the agar plate dilution method. The result shows that in the artificially polluted liquid inoculants, the survival rate of rhizobia was increased and the ratio of undesirable microbes declined significantly when the ampicillin was used as the bacteriostats. The growth of selected antibiotic-resistant strains was promoted at the concentration of less than 100 ug/ml and the doubling time was reduced, but inhibited and the doubling time was significantly prolonged at the concentration of more than 200 ug/ml. Inoculation tests also shows an increased nodulation competitiveness of two bacteriostat-resistant strains in five-fold dilutions of liquid inoculants containing ampicillin (at optimal concentration). According to comprehensive consideration, the optimum concentration for ampicillin as bacteriostat inRhizobiummeliloti LW107 andRhizobiumsp. RSW 96 inoculants appeared to be 100 ug/ml and 200 ug/ml, respectivly.

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