Antimicrobial activity of antiseptics in the prevention of postoperative infectious complications

Recently, among hospital strains of microorganisms, an increase in the number of antiseptic-resistant strains of opportunistic pathogens has been registered, which significantly affects the effectiveness of these drugs. It is important to study their antimicrobial efficacy to justify rational use. The aim is to conduct a comparative study of the antimicrobial efficacy of antiseptics of decamethoxine, chlorhexidine, polyhexanide. During study we examined the antimicrobial activity against 186 clinical strains of microorganisms (Acinetobacter baumannii, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus spp., Enterobacter spp.) isolated from patients with infectious complications in the postoperative period. The minimum inhibitory (MIC) and bactericidal concentrations (MBC) of 0.02 % and 0.1 % decamethoxine, 0.05 % chlorhexidine bigluconate, 0.1 % polyhexanide were determined; antimicrobial efficacy of drugs was evaluated by the index of antiseptic activity by conventional methods. The study found high antimicrobial properties of decamethoxine, chlorhexidine, which had a high bactericidal effect on clinical strains of S. aureus, Enterococcus spp., Enterobacter spp. Proved the benefits of antimicrobial activity of the drug based on decamethoxine (p<0.001). The polyhexanide has pronounced antimicrobial properties against A. baumannii, bacteria of the family Enterobactericae, P. aeruginosa. Thus, the leading gram-positive (Staphylococcus aureus, enterococci) and gram-negative pathogens (enterobacteria, acinetobacteria, pseudomonads) are sensitive to polyhexanide, chlorhexidine and the domestic drug decamethoxin, with a probable advantage of the antimicrobial properties of the latter over all gram-positive and most gram-negative microorganisms.

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Research of antimicrobial efficacy of modern antiseptic agents based on decamethoxine and povidone-iodine

Perioperaciina Medicina ◽

10.31636/prmd.v2i1.1 ◽

2019 ◽

Vol 2 (1) ◽

pp. 6-10 ◽

Cited By ~ 1

Author(s):

Oleksandr Nazarchuk

Keyword(s):

Antimicrobial Activity ◽

Povidone Iodine ◽

Antimicrobial Properties ◽

Infectious Complications ◽

Antimicrobial Efficacy ◽

Gram Positive ◽

Gram Negative ◽

Clinical Strains ◽

E Coli ◽

Enterococcus Spp

Under conditions of wide increased resistance of pathogens of infectious complications to antimicrobial agents, a considerable attention is paid to the use of antiseptic drugs. The research of their antimicrobial efficacy remains valid for substantiation of the rational use. The purpose was to carry out a comparative study of the antimicrobial efficacy of medicinal antiseptic agents based on decamethoxine and povidone-iodine. In the study there was examined antimicrobial activity of mentioned antiseptics against 682 clinical strains of microorganisms (A. baumannii, S. aureus, P. aeruginosa, Enterococcus spp., E. coli, Enterobacter spp., K. pneumoniae, Proteus spp.), isolated from patients with infectious complications. Minimum inhibitory and bactericidal concentrations of 1–2– 10 % iodine, 0.02 % decamethoxine (decasan) were determined. Antimicrobial efficacy of medicines, based on studied antiseptics was evaluated with the use of an index of antiseptic activity, calculated by means of commonly used methods. The study revealed high bactericidal properties of decasan against clinical strains of S. aureus, Enterococcus spp., E. coli, K. pneumoniae and Enterobacter spp. The advantages of antimicrobial activity of decamethoxine-based antseptis (decasan, p < 0.001) were proved. Iodine has expressed antimicrobial properties against Enterococcus spp., S. aureus, A. baumannii, bacteria of the Enterobacteriаcеae family and P. aeruginosa. It is proven that the dissolution of povidone-iodine leads to the reduction of the antimicrobial efficacy of 2 % antiseptic solution. There was found inefficacy of 1 % povidone-iodine against infectious agents (p < 0.001). Thus, the leading Gram-positive (S. aureus, Enterococci) and Gram-negative pathogens (Escherichia, Klebsiella, Enterobacteria, Acinetobacteria, Pseudomonas) have a sensitivity to iodine and to domestic preparation based on decamethoxin 0.02 % (decasan), with a definite advantage of the antimicrobial properties of the latter to Gram-positive and some Gram-negative microorganisms (p < 0.001).

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In Vitro Bactericidal Activity of Human β-Defensin 3 against Multidrug-Resistant Nosocomial Strains

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.50.2.806-809.2006 ◽

2006 ◽

Vol 50 (2) ◽

pp. 806-809 ◽

Cited By ~ 80

Author(s):

Giuseppantonio Maisetta ◽

Giovanna Batoni ◽

Semih Esin ◽

Walter Florio ◽

Daria Bottai ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bactericidal Activity ◽

Stenotrophomonas Maltophilia ◽

Bactericidal Effect ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Gram Negative

ABSTRACT The antimicrobial activity of human β-defensin 3 (hBD-3) against multidrug-resistant clinical isolates of Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Acinetobacter baumannii was evaluated. A fast bactericidal effect (within 20 min) against all bacterial strains tested was observed. The presence of 20% human serum abolished the bactericidal activity of hBD-3 against gram-negative strains and reduced the activity of the peptide against gram-positive strains.

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Synthesis and antimicrobial properties of new 2-((4-ethylphenoxy)methyl)benzoylthioureas

Chemical Papers ◽

10.2478/s11696-010-0092-9 ◽

2011 ◽

Vol 65 (1) ◽

Cited By ~ 9

Author(s):

Carmen Limban ◽

Alexandru Missir ◽

Ileana Chirita ◽

George Nitulescu ◽

Miron Caproiu ◽

...

Keyword(s):

Antimicrobial Activity ◽

Quantitative Methods ◽

Antimicrobial Properties ◽

Phenyl Group ◽

Inhibitory Effect ◽

Bacterial Strains ◽

Gram Positive ◽

Gram Negative ◽

Fungal Strains ◽

Qualitative And Quantitative Methods

AbstractNew acylthiourea derivatives, 2-((4-ethylphenoxy)methyl)-N-(phenylcarbamothioyl)benzamides, were tested by qualitative and quantitative methods on various bacterial and fungal strains and proved to be active at low concentrations against Gram-positive and Gram-negative bacteria as well as fungi. These compounds were prepared by the reaction of 2-((4-ethylphenoxy)methyl)benzoyl isothiocyanate with various primary aromatic amines, and were characterised by melting point and solubility. The structures were identified by elemental analysis, 1H and 13C NMR, and IR spectral data. The level of antimicrobial activity of the new 2-((4-ethylphenoxy)methyl)benzoylthiourea derivatives was dependent on the type, number and position of the substituent on the phenyl group attached to thiourea nitrogen. The iodine and nitro substituents favoured the antimicrobial activity against the Gram-negative bacterial strains, while the highest inhibitory effect against Gram-positive and fungal strains was exhibited by compounds with electron-donating substituents such as the methyl and ethyl groups.

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Phenylindolylmethyldiaminopyrimidines (PIDAPs) as Potent Antimicrobials Against Staphylococcus Aureus

10.26434/chemrxiv.13568669 ◽

2021 ◽

Author(s):

Margaret Deming ◽

Alatheia King ◽

Robert A Coover ◽

Cale D Fahrenholtz ◽

Aurijit Sarkar

Keyword(s):

Staphylococcus Aureus ◽

Structure Function ◽

Cell Lines ◽

Human Cell ◽

Antimicrobial Properties ◽

Penicillin G ◽

Human Cell Lines ◽

Gram Positive ◽

Gram Negative ◽

Dose Limiting Toxicity

We have ascertained that phenylindolylmethyldiaminopyrimidines (PIDAPs), stop the growth of USA300 MRSA at low micromolar concentrations. The controls, penicillin G and vancomycin, are able to stop the growth of MRSA at ~765 μM (256 μg/mL) and ~1.38 μM (2 μg/mL) respectively. We have also found out that PIDAPs are bactericidal at or close to the MIC. No activity was observed against Gram-negative pathogens. Other Gram-positive pathogens have not yet been tested. Based on a search through the ChEMBL database, PIDAPs are a novel class of chemicals with antimicrobial properties. A limited structure-function study suggests that the diaminopyrimidine is part of the pharmacophore. Unfortunately, we also detected potential dose-limiting toxicity on human cell lines. Further, detailed studies are needed.

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Green synthesis of silver nanoparticles using aqueous extract of Citrus sinensis peels and evaluation of their antibacterial efficacy

Green Processing and Synthesis ◽

10.1515/gps-2021-0061 ◽

2021 ◽

Vol 10 (1) ◽

pp. 851-859

Author(s):

Lebogang Mogole ◽

Wesley Omwoyo ◽

Elvera Viljoen ◽

Makwena Moloto

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Citrus Sinensis ◽

Antimicrobial Activities ◽

Antibacterial Efficacy ◽

Gram Positive ◽

Gram Negative ◽

Average Size ◽

Passivating Agent

Abstract The resistance of microorganisms towards antibiotics remains a big challenge in medicine. Silver nanoparticles (AgNPs) received attention recently for their characteristic nanosized features and their ability to display antimicrobial activities. This work reports the synthesis of AgNPs using the Citrus sinensis peels extract in their aqueous, mild, and less hazardous conditions. The effect of concentration variation (1%, 2%, and 3%) of the plant extracts on the size and shape of the AgNPs was investigated. The antimicrobial activities were tested against gram-positive Staphylococcus aureus and gram-negative Klebsiella pneumoniae. Absorption spectra confirmed the synthesis by the surface Plasmon resonance peaks in the range 400–450 nm for all the AgNPs. FTIR spectra confirmed that Citrus sinensis peels extract acted as both reducing and surface passivating agent for the synthesized AgNPs. TEM revealed spherical AgNPs with average size of 12 nm for 3% concentration as compared to the agglomeration at 1% and 2%. All the AgNPs synthesized using Citrus sinensis peels extracts (1%, 2%, and 3%) exhibited antimicrobial activity against both gram-positive and negative bacteria. These results indicated a simple, fast, and inexpensive synthesis of silver nanoparticles using the Citrus sinensis peels extract that has promising antibacterial activity.

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Synthesis, Characterization, and Antimicrobial Activity of Magnesium-Doped Hydroxyapatite Suspensions

Nanomaterials ◽

10.3390/nano9091295 ◽

2019 ◽

Vol 9 (9) ◽

pp. 1295 ◽

Cited By ~ 11

Author(s):

Daniela Predoi ◽

Simona Liliana Iconaru ◽

Mihai Valentin Predoi ◽

George E. Stan ◽

Nicolas Buton

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Spherical Shape ◽

Xrd Analysis ◽

Precipitation Method ◽

Gram Positive ◽

Gram Negative ◽

Morphological Studies ◽

Average Grain Size ◽

Co Precipitation

Obtaining nanoscale materials has allowed for the miniaturization of components, which has led to the possibility of achieving more efficient devices with faster functions and much lower costs. While hydroxyapatite [HAp, Ca10(PO4)6(OH)2] is considered the most widely used material for medical applications in orthopedics, dentistry, and general surgery, the magnesium (Mg) is viewed as a promising biodegradable and biocompatible implant material. Furthermore, Mg is regarded as a strong candidate for developing medical implants due to its biocompatibility and antimicrobial properties against gram-positive and gram-negative bacteria. For this study, magnesium-doped hydroxyapatite (Ca10−xMgx (PO4)6 (OH)2, xMg = 0.1), 10MgHAp, suspensions were successfully obtained by an adapted and simple chemical co-precipitation method. The information regarding the stability of the nanosized 10MgHAp particles suspension obtained by ζ-potential analysis were confirmed for the first time by a non-destructive ultrasound-based technique. Structural and morphological studies of synthesized 10MgHAp were conducted by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy in attenuated total reflectance (ATR) mode and scanning electron microscopy (SEM). The XRD analysis of the 10MgHAp samples confirmed that a single crystalline phase associated to HAp with an average grain size about 93.3 nm was obtained. The FTIR-ATR spectra revealed that the 10MgHAp sample presented broader IR bands with less visible peaks when compared to a well-crystallized pure HAp. The SEM results evidenced uniform MgHAp nanoparticles with spherical shape. The antimicrobial activity of the 10MgHAp suspension against gram-positive strains (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212), gram-negative strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853), as well as a fungal strain (Candida albicans ATCC 90029) were evaluated.

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Synthesis and Antimicrobial Properties of a Ciprofloxacin and PAMAM-dendrimer Conjugate

Molecules ◽

10.3390/molecules25061389 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1389 ◽

Cited By ~ 4

Author(s):

Søren Wedel Svenningsen ◽

Rikki Franklin Frederiksen ◽

Claire Counil ◽

Mario Ficker ◽

Jørgen J. Leisner ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Pamam Dendrimer ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative

Infections caused by bacteria resistant to antibiotics are an increasing problem. Multivalent antibiotics could be a solution. In the present study, a covalent conjugate between Ciprofloxacin and a G0-PAMAM dendrimer has been synthesized and tested against clinically relevant Gram-positive and Gram-negative bacteria. The conjugate has antimicrobial activity and there is a positive dendritic effect compared to Ciprofloxacin itself.

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Antibacterial Properties of Propolis

Molecules ◽

10.3390/molecules24112047 ◽

2019 ◽

Vol 24 (11) ◽

pp. 2047 ◽

Cited By ~ 33

Author(s):

Izabela Przybyłek ◽

Tomasz M. Karpiński

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Properties ◽

Bacterial Strains ◽

Complex Composition ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

The World

Researchers are continuing to discover all the properties of propolis due to its complex composition and associated broad spectrum of activities. This review aims to characterize the latest scientific reports in the field of antibacterial activity of this substance. The results of studies on the influence of propolis on more than 600 bacterial strains were analyzed. The greater activity of propolis against Gram-positive bacteria than Gram-negative was confirmed. Moreover, the antimicrobial activity of propolis from different regions of the world was compared. As a result, high activity of propolis from the Middle East was found in relation to both, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) strains. Simultaneously, the lowest activity was demonstrated for propolis samples from Germany, Ireland and Korea.

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Evaluation of Antimicrobial Activity of Cedrela toona Roxb. Fruit Extracts

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i1.4540 ◽

2021 ◽

Vol 11 (1) ◽

pp. 108-109

Author(s):

Kinjal H Shah ◽

Piyush M. Patel

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Bacterial Species ◽

Plate Method ◽

Gram Positive ◽

Gram Negative ◽

Negative Bacillus ◽

Gram Negative Bacillus

Evaluation of antimicrobial activity was performed by cup-plate method. The test microorganisms used for the antimicrobial activity were four bacterial species (two Gram positive and two Gram negative) – Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeuroginosa,Escherichia coli. The test microorganisms used for the antimicrobial activity were four bacterial species (two Gram +ve and two Gram -ve) Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeuroginosa,Escherichia coli.

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Antimicrobial Activity and Chemical Composition of the Essential Oil of Nepeta crispa Willd. from Iran

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-9-1008 ◽

2004 ◽

Vol 59 (9-10) ◽

pp. 653-656 ◽

Cited By ~ 38

Author(s):

Ali Sonboli ◽

Peyman Salehi ◽

Morteza Yousefzadi

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Essential Oil ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Total Oil ◽

And Staphylococcus Aureus

Abstract The composition and antimicrobial activity of the essential oil of Nepeta crispa Willd., an endemic species from Iran, was studied. The oil was obtained from the aerial parts of the plant and analyzed by GC and GC/MS. Twenty-three compounds, accounting for 99.8% of the total oil, were identified. The main constituents were 1,8-cineol (47.9%) and 4aα,7α,7aβ- nepetalactone (20.3%). The antimicrobial activity of essential oil of N. crispa was tested against seven gram-negative or gram-positive bacteria and four fungi. The results of the bioassays showed the interesting antimicrobial activity, in which the gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, were the most sensitive to the oil. Also, the oil exhibited a remarkable antifungal activity against all the tested fungi.

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