THE ECOLOGICAL FACTORS AND HUMAH HEALTH

One of the actual global problems of mankind is an environmental pollution and its negative impact on a population’s health, that is the basis of existence of each human being and the entire state. The purpose of this article is to inform the public about the environmental factors affecting human health and its causes.As there is significant rise of ecological diseases, in many countries worldwide during the last years a great attention is paid to the legal side of environmental protection. Public well being is determined by ensuring its sanitaryepidemiological conditions,safe environment and disease prevention.

SPECIFIC CHARACTERISTICS OF ELECTRON PARAMAGNETIC RESONANCE SPECTRUM OF DIFFERENT COLORS OF HAIR

In the result of interaction of the metabolic processes of the human organism and external factors free radicals are formed in tissues including hair. An objective assessment of an intensity of this process is possible by means of electronic paramagnetic resonance’s (EPR) signal and its intensity indices. The purpose of the survey was a comparative evaluation of the EPR spectrum’s specificity of black and brown hair and a study of a photo dynamic in the condition of a treating with blue light. The survey was conducted among young volunteers of age 17-21 on the base of their informed consent and with adherence of all bioethical requirements established for this type of research. No single person’s hair involved in the survey was treated with chemical dye or active, specific remedy for hair care. This article analyzes data of young men donors. All measurements were carried out at standard conditions: room temperature (22-240C), humidity - 60-70%, the measurement error - ± 5%. EPR spectrum was determined at the Department of Biophysics,TSU by means of a radio-spectrometer TSR - V. Study results have determined a uniform EPR spectrum and no difference in the intensity of the photoinduced EPR spectrum that indicates the equal concentrations of eumelanin in black and brown hair.

ABOUT THE MATTER OF LABOUR CONDITIONS CLASSIFICATION IN TBILISI SUBWAY

The aim of this research was the development of the hygienic classification of labour conditions of workers of the Tbilisi subway by indicators of harm and danger of work on the basis of appropriate standard document. The complex of adverse factors of the labour environment which is a potential risk for workers’ health is revealed. It is established that the most adverse, i.e. extreme working conditions (a class of danger 4) are noted on a workplace of a machinist, that is generally caused by parameters of noise and vibration (a class of danger 3 and 4). In the distribution hall and on a workplace of the controller on duty a working condition corresponds 3. 1 class of danger, and on other workplaces to 3.2 class. The results of the designated research are used for development of complex improving measures, which include technological, sanitary and hygienic and medico-preventive measures

PERSPECTIVE OF PHOTOTHERAPY OF DERMATOSES BY MEANS OF UVB -311 NM

The paper presents the modern data concerning the spectrum of electromagnetic radiation and its impact on the structure and function of the skin at various dermatoses. The possibilities of non-drug treatment of dermatoses using different UV spectrum are described. Particular attention is paid to the study of the most effective and safe method for treatment by means of narrow-wave phototherapy with a maximum emission at a wavelength of 311 nm (UVB - 311). The results of the comparative analysis of efficacy and safety of PUVA photochemotherapy and phototherapy UVB -311, on examples of some specific nosologic units (psoriasis, parapsoriasis, vitiligo, mycosis fungoides, photodermatosis, atopic dermatitis) are presented. Based on analysed data, authors conclude that UVB -311 nm is an effective, well-tolerated, safe and alternative method for treatment of chronic dermatoses. The brief review of the available in literature data, allow authors to make conclusion about the possibility of widespread implementation of phototherapy method in practical dermatology.

THE CHANGES IN CLONES OF ANTIGENEREACTIVE IMMUNE LYMPHOCYTES TO DIFFERENT MICROORGANISMS DURING PARODONTITIS

The activation of clones of immune lymphocytes to streptococci takes place at parodontitis. The number of noted lymphocytes significantly increases in the blood of patients with parodontitis as compared to healthy persons having intact parodontium (12.7±0.3 versus 3.87±0.32, P<0.001). At various forms of parodontitis the growth of antigenreactive lymphocytes is expressed with different degrees. Particularly sharp increase is observed during severe forms of parodontitis as compared to average and light forms (the number of antigenereactive lymphocytes to streptococci in the blood respectively made up 16.9±0.59, 12.8±0.23, 9.5±0.27, P<0.001). As to immune lymphocytes to staphylococci and actinomycete, their activation was not revealed at parodontitis. The quantitative indices of the noted clones of lympho- cytes in the blood did not essentially differ from analogical indices of healthy individuals (an average amount of immune lymphocytes to staphylococci and fungi made up 4.3±0.26 and 3.84±0.29, P<0.05 as compared to healthy individuals). Thus, the increase of number of lymphocytes having receptors to streptococci in the blood has been first established at parodontitis and its intensity was in direct correlation to the degree of the generalization of the process. Proceeding from the aforesaid, it is possible to think that the leukocytes accumulated as a result of chemotaxis in response to microbial aggression in the inflammatory focus, developed in the tissues of parodontium, especially neutrophils and macrophages induce the disorganization of streptococci and their antigens, including the release of antigens common to the tissues of the organism, followed by the development of reciprocal protective and destructive immune processes.

LABORATORY EVALUATION OF THE EFFECTIVENESS OF TREATMENT WITH THE VECTOR SYSTEM

Introduction of new methods of diagnosis and treatment of periodontal complex diseases in practice represents priority issues for modern dentistry. Diagnosis of the disease is based on clinical and laboratory findings.The active growth of bacteria in plaque and the appearance of increased virulence of periodontal pathogens is an important factor in the development of Periodontitis. Periodontal complex disease treatment depends on the type and progress of diseases, it is necessary to eliminate pathogens and carry out symptomatic treatment, which has been successfully achieved by conservative treatment with Vector system. Periodontal therapy by means of Vector device directly affects an environment of the tooth. It allows removing sub gingival dental plaque destroying pathogenic microorganisms and their toxins, washing out periodontal pockets carefully and polishing teeth roots. During treatment the hard tissues as well as gum are not injured. Periodontal therapy with the ultrasonic device leads to clinical improvements. It was found that Vector treatment was effective in the treatment of patients suffering from periodontitis.

DETERMINATION OF IN VITRO OUTLET OF LIZINOPRIL CONTAINING MEDICAL PREPARATION ”LIZINOCOR” AND ITS ANALOGUE USING METHOD OF HIGHPERFORMANCE LIQUID CHROMATOGRAPHY

ACE Inhibitors represent the first class of anti hypertensive agents that was designed and developed on the basis of a well-defined physiopathol ogical axis of arterial hypertension, a vascular disorder that is now becoming one of the major causes of morbidity/mortality, not only in developed societies but also in the highly populated developing countries. An angiotensin-converting enzyme (ACE) inhibitor, the carboxylalkyl compounds derivated lizinopril is apharmaceutical drug, used primarily for the treatment of hypertension (elevated blood pressure) and congestive heart failure. Purpose of investigation was – comparison of in vitro outlet of 5 mg tablets of ”Lizinocor” containing Lizinopril , produced by the Georgian pharmaceutical company “GMP” and its analogue ”Zestril” using method of high-performance liquid chromatography. According to the received results average percent quantity outlet of ”lizinocor” is 97,65 %, ”Zestril” – 95, 88%;. Inclination in comparison with Zestril is 1,85 %, (norm ± 5 %). ”Lizinocor” 5 mg tablets containing Lizinopril produced by the Georgian pharmaceutical company ”GMP” are characterized by good outlet quality.

EVELOPMENT OF GAS CHROMATOGRAPHY-MASSSPECTROMETRIC (GC-MS) METHOD OF DETERMINATION OF TETRAHYDROCANABINOL AND 11-NORTERAHYDROCANABINOL CARBOXYACIDE IN BIOLOGICAL FLUIDS

In chemichal-toxicological analysis was used the method of Gas Chromatography-Mass Spectrometry. It is considered as one of the most exact method of analysis of chemichal substances. Respectively utilization of this method in determination of widely spread narcotic in biological fluids is important task. As a result of experimental work, was determined optimal conditions of analysis: temperature of injector, fuel and transferline, the thermal gradient, volume of injection, length of the column, thickness of the layer, gass-wearing speed of mobile phase. Detection of Desomorphine was carried by TIC and SIM regime, under the NIST data base. For derivatisation was selected acitilation (acetate anhydride), metilation and sylitation were performed. The optimal sylitation of tetrahydrocanabinol and 11nor-terahydrocanabinol carboxyacide was performed by BSTFA. In the current work is presented the chromatograme and mass-specter of tetrahydrocanabinol and 11-nor-terahydrocanabinol carboxyacide detected in urine.

ANALYSIS OF METABOLITES OF SYNTHETIC CANNABINOIDS JWH-018 AND JWH-073 BY CHROMATOGRAPHYMASS-SPECTROMETRIC (LC-MS/MS)METHOD IN BIOLOGICAL FLUIDS

A liquid chromatographic method was developed to resolve a comprehensive set of metabolites of JWH-018 and JWH-073. In addition to the chromatographic analysis method, an extraction method was developed to recover a broad range of synthetic cannabinoid metabolites, including carboxylic acid metabolites that are not traditionally recovered using a high pH liquid/liquid extraction. The extraction and analysis methods were used to identify and quantify the significant metabolites in urine samples. The chromatographic method detailed in this application note employed a RP-HPLC column and MS/MS detection. The quantitative range validated for all metabolites was 1 ng/mL to 500 ng/mL in urine. Based on the data, this method is suitable for quantifica- tion of metabolites of JWH-018 and JWH-073 to support broader research studies that positively identify clinically significant metabolites and their concentrations in urine.

PROFILE OF COLIFORM STRAINS’ ANTIBIOTIC RESISTANCE

The spectrum of sensitivity of 112 E. coli strains isolated from patients with acute intestinal infection to 23 antibiotics was investigated by the agar dilution method, which revealed an increase in drug resistance and the emergence of multidrug-resistant strains (33.04%). It was found that the drug resistance of the pathogen to nalidixic acid and carbopenems increased and its high sensitivity to some ftuoroquinolones, III and IV generation aminoglycosides, penicillins and cephalosporins preserved. The drug resistance in future may lead to the formation of hospital strains among E. coli and alter an epidemiological process and the clinical course of the disease.