scholarly journals Anatomical study of the leaves and petioles of scab resistant and susceptible apple cultivars

2005 ◽  
Vol 11 (3) ◽  
Author(s):  
M. Tóth ◽  
P. Gracza

Anatomic studies have been performed on the leaf blade, petiole and annual shoot on six apple cultivars by means of scanning electron as well as light microscope. Four of the cultivars examined are resistant to scab (Florina, Freedom, MR-10, MR-11), whereas two of them are susceptible (Jonathan and Idared). Preliminary results suggest that differences in the width of cross sections of leaf blades, in hairyness, in the shape and size of epidermal cells, moreover, in the cross sections of petioles and shoots are considerable. Some of the anatomical properties seem to be correlated with scab resistance or susceptility of the respective cultivars. Therefore, further studies extending to other cultivars may corroborate our claims to find causal relations between anatomical traits of the leaves and disease, especially scab resistance of apple cultivars.

2010 ◽  
Vol 16 (5) ◽  
Author(s):  
D. Varga ◽  
L. Udvardy

According to previous studies some anatomical features seem to be connected with resistance or susceptibility to scab caused by Venturia ineaqulis (Cke./Wint.) in case of a given cultivar. Study of leaf anatomy of three scab resistant (‘Prima’, ‘Florina’, MR–12) and two susceptible (‘Watson Jonathan’, ‘Golden Delicious Reinders’) apple cultivars have been made. Preserved preparations made of leaves has been studied by light microscope. Studied parameters were: thickness of leaf blade, thickness of palisade and spongy parenchyma, thickness of epidermal cells, thickness of the cuticle. By measuring leaf thickness and epidermal cell thickness visible differences appeared in certain cultivars, while most conspicuous difference has been shown in thickness of the cuticle.


2020 ◽  
Vol 49 (3) ◽  
pp. 633-641
Author(s):  
Onur Koyuncu

Achillea ketenoglui H. Duman is an endangered species in Turkey. Its morphological, anatomical and palynological features were studied. Microphotographs of the cross sections of stem and root of this species are provided along with the detailed anatomical features and their morphological description with the help of drawings. Distribution and ecology of A. ketenoglui were determined and IUCN risk category was revised. Microphotographs of scanning electron and light microscope was studied, evaluated and discussed for their taxonomic use.


2012 ◽  
Vol 59 (2) ◽  
pp. 69-83
Author(s):  
Elżbieta Weryszko-Chmielewska ◽  
Magdalena Gantner ◽  
Jadwiga Kostrzewska-Kuczumow

Investigations related to morphological and anatomical traits of leaves of four largefruited hazelnut (<i>Corylus</i> L.) cultivars characterised by different susceptibility to filbert aphid (<i>Myzocallis coryli</i> Goetze) feeding. The following parameters were measured: the thickness of leaf blade, the number of stomata, the length of secretory and mechanical hairs and their density on the leaf, the thickness of adaxial and abaxial epidermis and the thickness of their external cell walls. Observations of cross sections of the leaves were made in a light microscope and the surface of the adaxial epidermis was analysed in a scanning electron microscope. It was shown that leaves of the cultivars susceptible to <i>M. coryli</i> feeding had the thinnest leaf blade, especially in the main vein, and many more stomata. Besides, the cultivar most resistant to filbert aphid feeding, White Filbert, was marked by the largest height of the adaxial epidermis cells and the strongest striation of the cuticle in abaxial epidermis. On leaves of this cultivar, the number of mechanical hairs was the highest, while the lowest number of them was noted on Wonder from Bollwiller, the most susceptible cultivar.


Author(s):  
Khazal Dibba Wadi - Zeinab Nasrallah Salman

The current study is a comparative anatomical study of Diplotaxis spp DC species of the Brassicaceae which are growing in Diyala province { Diplotaxis acris (Fossk.) Boiss. Diplotaxis erucoid (L.) DC, Diplotaxis harra (Forssk) Boiss}. The anatomical study included the dissection of the stems, the petioles and the leaf blade. The results of the present study showed that the anatomical characteristics have a great taxonomic significance, which helps in isolation and diagnosis, through the many variations shown by the anatomical characteristics of the studied species. The stem showed significant anatomical properties in terms of the shape of the cross section. D.rucoid was characterized as having ribbed stems and was characterized by the presence of collenchyma cells at the corners of the ribs to give support and attribution as well as flexibility in motion. D.harra also characterized epidermis cells as rectangular. The cortex also varied in the species of cells, the number of rows of chlorenchyma and paranchyma, and the number and distribution of vascular bundles, the number of arms of wood and the number of vessels in each arm. The variances included the cross section of the leafblade and the shape of the cross-section was important in isolation and diagnosis. It also varied in thickness, epidermis, vascular forms, numbers, distribution, cortex thickness and layers. The leaves were Monoficial in type D.harra It is also characterized by its lack of sponge cells while the two species of D. acris and D. erucoid were Bificial. The vertical sections of leaves, especially the mesophyll, varied widely, in the thickness of blade, dermis, epidermis ,forms of the central vascular bundles and the preparation of the arms and elements. All these qualities have contributed as an addition of distinctive qualities to each important species in its isolation and diagnosis.


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 678c-678
Author(s):  
Carlos H. Crisosto ◽  
Juvenal G. Luza ◽  
David Garner ◽  
Guiwen Cheng ◽  
Kevin Day

Peach and nectarine skin discoloration or inking (SD) has become a fruit industry problem in the last decade. Spots on the skin may be black, tan, purple or brown and vary in shape. SD was related with physical abuse of the fruit occurring during handling (harvest and transport operations) within the orchard. An anatomical study comparing healthy and damaged (black and brown) tissue of different peach and nectarine varieties was done with the Scanning Electron Microscope (SEM) and Light Microscope (LM). This study indicated that only exocarp cell (epiderm and cuticle) damage was associated with SD. The internal compartmentation of the damaged cells was often disrupted with the contents of the cytoplasm and vacuole mixed and expelled. Mesocarp cells were always intact and turgid. The same anatomical and visible tissue injury symptoms were induced on fruit by abrasion treatments.


2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Kufre Edet Okon ◽  
Michael Akpan ◽  
Idiana-Abasi Kufre Okon ◽  
Uduak Oworen

Wood modification in general, is an environmental non-biocide method to modify and improve certain wood properties to enable its utilization for diverse purpose. In this study, Firmiana simplex wood was modify using low melting point tin-alloy as the heat-treatment medium. The anatomical properties of the tin-bath treated wood were studied using a light microscope and scanning electron microscope analysis. Microscopic analyses with a light microscope showed that the morphologies of the wood were less smashed by the impact of the treatment with no deformation of the vessels after tin-alloy treatment while scanning electron microscope revealed collapse in the wood structures with damages to the cell walls and vessels. It was also evident from scanning electron microscope analyses that tin-alloy penetrated the wood structure, therefore forming a film covering the cell walls and partly occupying the wood cell lumens.


2010 ◽  
Vol 18 (3) ◽  
pp. 54-57
Author(s):  
Steve Chapman

If you wish to look inside an arbitrary piece of material and you own a light microscope (LM) or scanning electron microscope (SEM), there are probably three ways that you may accomplish your aim: (a) embed the material in epoxy, then grind and polish it flat; (b) embed the material and use a microtome to obtain cross sections; or (c) CRACK IT. In this short article, it is the last option that will be discussed. The last method is the simplest and quickest of the textbook sample preparation methods for SEM .


2012 ◽  
Vol 60 (1) ◽  
pp. 35-43
Author(s):  
Agata Konarska

The study of floral nectary structures of thirteen ornamental apple cultivars examined using light microscope (MS) and scanning electron microscope (SEM) was performed. It was found that nectary glands in the selected cultivars were located in the upper part of the flower receptacle, between the ovary of the pistil and the base of stamen filaments, and they generally belonged to the epimorphic or transitoric type. The nectary surface area, its thickness, the number of glandular tissue layers, the height of epidermal cells of the nectary and the thickness of the outer wall of the epidermis, together with the cuticle, were determined by light microscope. By using SEM, the structure of the surface of nectaries in four ornamental apple cultivars was observed. The epidermis of the upper part of the nectaries was composed of elongated cells of which outer cell wall was covered with a striated cuticle. The remaining part of the nectary was characterised by cells of similar arrangement and shape, but their surface was marked by a thinner and smoother layer of cuticle. Closed or opened stomata were generally situated at the level of the epidermal cells. Their pores were often filled with granular or plate-shaped structures.


Genetika ◽  
2019 ◽  
Vol 51 (3) ◽  
pp. 1175-1184
Author(s):  
Biljana Nikolic ◽  
Zorica Mitic ◽  
Srdjan Bojovic ◽  
Vlado Matevski ◽  
Zoran Krivosej ◽  
...  

Eight morpho-anatomical properties of two-year-old needles of Pinus heldreichii (Bosnian pine) from the Scardo-Pindic mountain massif in Serbia (Kosovo, Mt. Osljak) and North Macedonia (Mt. Galicica) were investigated. All measured characteristics, except for needle length, were inspected on mid-needle cross-section. Cross-sections were obtained with razorblade, while measurements were performed with Leica-Gallen III light microscope. The mean values of the analyzed characters were as follows: 5.91 cm (needle length), 1.35 mm (needle width), 0.85 mm (needle thickness), 25.05 ?m (cuticle + epidermis thickness), 69.90 ?m (height of hypodermal cells), 21.76 ?m (resin duct diameter), 3.4 (number of hypodermis layers), and 3.6 (number of resin ducts). P. heldreichii needles also had 2-5 hypodermis layers and 0-12 resin ducts. The highest variation was in the number of resin ducts (CV=27%). In comparison with previously investigated needles from the Dinaric mountains, P. heldreichii needles from the Scardo-Pindic massif were shorter and had a thicker layer of hypodermis. PCA and CA visualize partial segregation of P. heldreichii populations between the two mountain massifs.


Author(s):  
R. E. Ferrell ◽  
G. G. Paulson

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.


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