scholarly journals In Vitro Antibacterial Activity of Eurycoma Longifolia Jack (Tongkat Ali) Root Extract

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Faisal GG ◽  
Zakaria SM ◽  
Najmuldeen GF
Keyword(s):  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Root Extract ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Eurycoma Longifolia ◽  
Herbal Plants ◽  
Eurycoma Longifolia Jack

Introduction: Currently, researchers are aiming to explore herbal plants to replace synthetic drugs because herbal plants contain high active compounds and fewer side effects. Our study was done to determine the antibacterial activity of Eurycoma longifolia Jack (E. longifolia) root using ethanol based extract. Methods: Five types of pathogenic bacterial strains were used; Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa). Disc diffusion assay and Minimum Inhibitory Concentration (MIC) tests were used to determine the inhibition zone and turbidity of suspension which reflects the antibacterial activity of the extract. Results: The ethanolic extract of E. longifolia Jack root extract showed positive results against Gram-positive bacteria (S. aureus and B. cereus) and Gramnegative (S. typhi). B.cereus and S.typhi showed inhibition zone values of 11.76mm and 14.33mm at the extract concentration of 150mg/ml that were higher than the positive control values (9.00, 12.67mm) respectively. However, E. coli and P. aeruginosa did not show any inhibition by the ethanol-based extract. Conclusion: From the results we can conclude that E.Longifolia root extract possesses antibacterial activity that can be further explored to produce new medicinal products.

scholarly journals Antibacterial Activity of Floral Petals of Some Angiosperms

2019 ◽  
pp. 1-5 ◽  
Author(s):  
L. Rajanna ◽  
N. Santhosh Kumar ◽  
N. S. Suresha ◽  
S. Lavanya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Zone ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Assay

The in vitro antibacterial assay was carried out against both Gram positive (B. cerus and S. aureus) and Gram negative (E. coli and K. pneumoniae) bacteria. Floral petals of 20 different species of plants were collected and tested for antibacterial activity. The result showed that the petals were active against both Gram positive and Gram negative. Out of 20 floral petals tested, 19 floral petals exhibited antibacterial activity against selected bacterial strains. The minimal inhibitory zone of floral petal discs against human pathogenic bacteria varies from 2 – 6 mm. Rosa carolina and Ruellia tuberosa showed significance inhibition zone for all the bacterial strains while Lantana camara does not show inhibition zone for any of these pathogenic bacteria.

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

scholarly journals Design, Synthesis, and Antibacterial Screening of Some Novel Heteroaryl-Based Ciprofloxacin Derivatives as DNA Gyrase and Topoisomerase IV Inhibitors

2021 ◽  
Vol 14 (5) ◽  
pp. 399
Author(s):  
Lamya H. Al-Wahaibi ◽  
Amer A. Amer ◽  
Adel A. Marzouk ◽  
Hesham A.M. Gomaa ◽  
Bahaa G. M. Youssif ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Dna Gyrase ◽  
Bacterial Strains ◽  
Topoisomerase Iv ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Screening ◽  
Ic50 Values

A novel series of ciprofloxacin hybrids comprising various heterocycle derivatives has been synthesized and structurally elucidated using 1H NMR, 13C NMR, and elementary analyses. Using ciprofloxacin as a reference, compounds 1–21 were screened in vitro against Gram-positive bacterial strains such as Staphylococcus aureus and Bacillus subtilis and Gram-negative strains such as Escherichia coli and Pseudomonas aeruginosa. As a result, many of the compounds examined had antibacterial activity equivalent to ciprofloxacin against test bacteria. Compounds 2–6, oxadiazole derivatives, were found to have antibacterial activity that was 88 to 120% that of ciprofloxacin against Gram-positive and Gram-negative bacteria. The findings showed that none of the compounds tested had antifungal activity against Aspergillus flavus, but did have poor activity against Candida albicans, ranging from 23% to 33% of fluconazole, with compound 3 being the most active (33% of fluconazole). The most potent compounds, 3, 4, 5, and 6, displayed an IC50 of 86, 42, 92, and 180 nM against E. coli DNA gyrase, respectively (novobiocin, IC50 = 170 nM). Compounds 4, 5, and 6 showed IC50 values (1.47, 6.80, and 8.92 µM, respectively) against E. coli topo IV in comparison to novobiocin (IC50 = 11 µM).

scholarly journals Formulation and evaluation of herbal ointment using leaves of Thuja orientalis l. Leaf extract

2021 ◽  
Vol 3 (2) ◽  
pp. 001-008
Author(s):  
Krishanu Samanta
Keyword(s):  
Antibacterial Activity ◽  
Research Work ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Alternative Formulation ◽  
Traditional System ◽  
Herbal Plants ◽  
Thuja Orientalis ◽  
Is Design

The study of diseases and their treatment are important part of our ancient time worldwide. The knowledge of medicinal plants must have been accumulated in the course of many centuries. Herbal plants are huge sources of neutraceuticals, prevent the different disease or maintain healthy life. Plant produces primary metabolites for their basic survival and secondary metabolites for their ecological, taxonomical and biochemical differentiation and diversity. Herbal medicine prepare different part of plant are used. Herbal drug is design as the alternative formulation for the external use in the form of ointment. For the clinical use the herbal ointment apply externally on human body. The main aims of this research are preparation of herbal ointment from the Thuja orientalis L.Plants used as antibacterial activity. Under this research work, it is design as herbal ointment with the help of Ethanolic extract of Thuja orientalis L. and determines the anti bacterial capacity on it. In vitro study the antibacterial activity of the Ethanolic extracts of dried leaves of Thuja orientalis L. are determine by using the Agar cup plate method versus different bacteria such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa etc. By blending the Ethanolic extract of Thuja orientalis L.(10 % w/w) into aqueous cream we formulated herbal ointment. The emulsifying agent and simple ointment bases are added in above herbal ointment for increase the antibacterial effectiveness during in vitro evaluation. Thuja orientalis L. (Family Cupressaceae) are broadly used in traditional system of medicine throughout different part of India, China, Russia, Korea, Japan, India, Florida and Iran. It is used in the treatment of diarrhea, cough, cold, bronchitis, and upper part repertory infection. The current study deals with the determination of formulation and evaluation of herbal ointment using Thuja orientalis leave extract.

scholarly journals In vitro Evaluation of Antibacterial Activity and Protective Effects on Cardiomyocytes with H/R Injury of Garlic/Onion Oil

2020 ◽  
Author(s):  
Yifei Lu ◽  
Hui Lv ◽  
Lihua Li ◽  
Chao Yang ◽  
Da Pan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Survival Rate ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Protective Effects ◽  
Volatile Oils ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Garlic Oil

Abstract Background: At present, the research concerning in vitro experiments of garlic oil and onion oil is limited. The objective of this study was to carry out the research on the effective components of garlic and onion, and to study the antibacterial activity of the two essential oils and their protective effects on cardiomyocytes with hypoxia/reoxygenation (H/R) injury.Method: The protective effect of volatile oils at different concentrations (200, 100, 50, 25, 12.5, 6.3, 3.1mg/L) on the endothelial cell of microvascular in cardiac muscle against H/R injury was examined by MTT and flow cytometer method. The survival rate and apoptosis situation were record. Inhibition zone test was used to evaluate antibacterial activity of volatile oils. MIC and MBC were calculated as well.Results: To achieve a higher survival rate, the optimum concentrations of garlic oil and onion oil were 12.5mg/L and 6.13mg/L and high concentrations of garlic and onion oil decreased the survival rate. Both the 12.5mg/L onion and garlic oil reduced the apoptosis situation of cardiomyocytes. Both garlic oil and onion oil had antibacterial effect on gram-positive bacteria and gram-negative bacteria, and had the best antibacterial effect on cocci of gram-positive bacteria.Conclusion: Garlic and onion oil do have antibacterial activity and protective effects on cardiomyocytes with H/R injury in a concentration range.

scholarly journals Antibacterial activity of cobalt(II) complexes with some benzimidazole derivatives

2008 ◽  
Vol 73 (12) ◽  
pp. 1153-1160 ◽  
Author(s):  
S.O. Podunavac-Kuzmanovic ◽  
V.M. Leovac ◽  
D.D. Cvetkovic
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Antibacterial Activities ◽  
Benzimidazole Derivatives ◽  
Wall Structure ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

The antibacterial activities of cobalt(II) complexes with two series of benzimidazoles were evaluated in vitro against three Gram-positive bacterial strains (Bacillus cereus, Staphylococcus aureus, and Sarcina lutea) and one Gram-negative isolate (Pseudomonas aeruginosa). The minimum inhibitory concentration was determined for all the complexes. The majority of the investtigated complexes displayed in vitro inhibitory activity against very persistent bacteria. They were found to be more active against Gram-positive than Gram-negative bacteria. It may be concluded that the antibacterial activity of the compounds is related to the cell wall structure of the tested bacteria. Comparing the inhibitory activities of the tested complexes, it was found that the 1-substituted- -2-aminobenzimidazole derivatives were more active than complexes of 1-substituted- 2-amino-5,6-dimethylbenzimidazoles. The effect of chemical structure on the antibacterial activity is discussed.

scholarly journals PHYTOCHEMICAL SCREENING OF DIFFERENT ROOT EXTRACTS OF Ageratum conyzoides AND THEIR POTENTIAL BIOACTIVE PROPERTIES

2021 ◽  
Vol 9 (5) ◽  
pp. 639-646
Author(s):  
Tanveer Ahmad ◽  
◽  
Tahir Rasheed ◽  
Sarmad Ahmad Qamar ◽  
Muhammad Bilal ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Point Of View ◽  
Root Extract ◽  
Bacterial Strains ◽  
Ageratum Conyzoides ◽  
Gram Positive ◽  
Gram Negative ◽  
Root Extracts ◽  
Antibacterial And Antifungal

The present study was conducted to determine different classes of secondary metabolites of Ageratum conyzoides root extracts and to evaluate their antioxidant, antibacterial, and antifungal potentialities using various pathogenic fungal and different gram-positive/gram-negative bacterial strains. The roots powder was subjected to ultrasonic-assisted extraction with n-hexane, acetone, ethanol, and EtOH:H2O(1:1). The screening of phytochemicals indicated the existence of terpenoids, alkaloids, coumarins, sterols, flavonoids, and glycosides in the root extract of A. conyzoides. However, the absence of saponins, tannins, anthocyanidins, anthraquinones, and phlobatannins was observed. The results indicated a reasonable antibacterial (against gram-negative and gram-positive bacteria), and antifungal potential. The antibacterial activity of the ethanolic extract was highest against all four strains of bacteria and was also comparable to the standard medicines used. However, antifungal activity was highest in EtOH:H2O (1:1) extract. Moderate antioxidant properties were also demonstrated, favoring the importance of the roots of this plant from a medicinal point of view.

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