scholarly journals Development of a Single-Pulse Femtosecond X-ray Imaging Technique for Samples in Liquids.

2020 ◽  
Vol 40 (159) ◽  
pp. 26-28
Author(s):  
Takashi KIMURA
Author(s):  
M.G. Baldini ◽  
S. Morinaga ◽  
D. Minasian ◽  
R. Feder ◽  
D. Sayre ◽  
...  

Contact X-ray imaging is presently developing as an important imaging technique in cell biology. Our recent studies on human platelets have demonstrated that the cytoskeleton of these cells contains photondense structures which can preferentially be imaged by soft X-ray imaging. Our present research has dealt with platelet activation, i.e., the complex phenomena which precede platelet appregation and are associated with profound changes in platelet cytoskeleton. Human platelets suspended in plasma were used. Whole cell mounts were fixed and dehydrated, then exposed to a stationary source of soft X-rays as previously described. Developed replicas and respective grids were studied by scanning electron microscopy (SEM).


2004 ◽  
Vol 37 (5) ◽  
pp. 757-765 ◽  
Author(s):  
L. E. Levine ◽  
G. G. Long

A new transmission X-ray imaging technique using ultra-small-angle X-ray scattering (USAXS) as a contrast mechanism is described. USAXS imaging can sometimes provide contrast in cases where radiography and phase-contrast imaging are unsuccessful. Images produced at different scattering vectors highlight different microstructural features within the same sample volume. When used in conjunction with USAXS scans, USAXS imaging provides substantial quantitative and qualitative three-dimensional information on the sizes, shapes and spatial arrangements of the scattering objects. The imaging technique is demonstrated on metal and biological samples.


2012 ◽  
Vol 452-453 ◽  
pp. 83-89 ◽  
Author(s):  
Sung Yong Jung ◽  
Seungmin Lim ◽  
Sang Joon Lee

2014 ◽  
Vol 85 (3) ◽  
pp. 033502 ◽  
Author(s):  
K. Nishimura ◽  
A. Sanpei ◽  
H. Tanaka ◽  
G. Ishii ◽  
R. Kodera ◽  
...  

2014 ◽  
Vol 47 (1) ◽  
pp. 188-197 ◽  
Author(s):  
Changyong Song ◽  
Kensuke Tono ◽  
Jaehyun Park ◽  
Tomio Ebisu ◽  
Sunam Kim ◽  
...  

X-ray free-electron lasers (XFELs) provide intense (∼1012 photons per pulse) coherent X-rays with ultra-short (∼10−14 s) pulse lengths. X-rays of such an unprecedented nature have introduced new means of atomic scale structural investigations, and discoveries are still ongoing. Effective use of XFELs would be further accelerated on a highly adaptable platform where most of the new experiments can be realized. Introduced here is the multiple-application X-ray imaging chamber (MAXIC), which is able to carry out various single-pulse diffraction experiments including single-shot imaging, nanocrystallographic data acquisition and ultra-fast pump–probe scattering for specimens in solid, liquid and gas phases. The MAXIC established at the SPring-8 ångström compact free-electron laser (SACLA) has demonstrated successful applications in the aforementioned experiments, but is not limited to them. Also introduced are recent experiments on single-shot diffraction imaging of Au nanoparticles and serial crystallographic data collection of lysozyme crystals at SACLA.


2013 ◽  
Vol 20 (2) ◽  
pp. 324-331 ◽  
Author(s):  
Sung Yong Jung ◽  
Sungsook Ahn ◽  
Eunseok Seo ◽  
Sang Joon Lee

2013 ◽  
Vol 17 (7) ◽  
pp. 507-514 ◽  
Author(s):  
Y. Kobayashi ◽  
H. Inose ◽  
R. Nagasu ◽  
T. Nakagawa ◽  
Y. Kubota ◽  
...  

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