scholarly journals Efektifitas Ekstrak Jahe Merah (Zingiber officinale var. rubrum) Terhadap Infeksi Bakteri Vibrio Alginolyticus Pada Ikan Kerapu Macan (Epinephelus Fuscoguttatus Forsskål, 1775) Secara In Vitro

2019 ◽  
Vol 1 (1) ◽  
pp. 27-31
Author(s):  
Yanti Muthalib ◽  
Asmi Citra Lestari ◽  
Alexander Rante Tondok ◽  
Admi Athirah
Keyword(s):  
Zingiber Officinale ◽  
Vibrio Alginolyticus ◽  
Epinephelus Fuscoguttatus

Penelitian ini bertujuan mengetahui pengaruh ekstrak jahe merah dengan kosentrasi yang berbeda terhadap pertumbuhan bakteri patogen V. Alginolyticus pada ikan kerapu macan.  Isolasi bakteri V. alginolyticus dilakukan di laboratorium Mikrobiologi Laut.  Uji in- vitro ekstrak jahe merah dilakukan di Laboratorium Biofarmaka Universitas Hasanuddin.  Pengujian antibakteri menggunakan metode difusi agar. Hasil uji difusi ekstrak n-Heksana, etanol dan metanol menunjukan bahwa ekstrak metanol memiliki nilai tertinggi 15,19 mm.  Pada pengujian konsentrasi hambat minimum (KHM) ekstrak metanol  terhadap bakteri patogen V.alginolyticus BBAP diperoleh KHM pada konsentrasi 250 ppm.  Karena pada konsentrasi dibawah 250 ekstrak metanol jahe merah sudah tidak memilik zona hambat sama sekali.

scholarly journals FITOFARMAKA DAUN SAMBUNG NYAWA (Gynura procumbens) UNTUK MENINGKATKAN IMUNITAS IKAN KERAPU MACAN (Epinephelus fuscoguttatus Forsskal 1775) TERHADAP SERANGAN BAKTERI Vibrio alginolyticus

2019 ◽  
Vol 12 (1) ◽  
pp. 52
Author(s):  
Etika Oktaviani ◽  
Esti Harpeni ◽  
W Wardiyanto
Keyword(s):  
Vibrio Alginolyticus ◽  
Epinephelus Fuscoguttatus

<p>BSTRAK<br />Fitofarmaka di Indonesia sudah tidak asing lagi dan banyak dimanfaatkan untuk pengobatan-pengobatan tradisional. Tanaman sambung nyawa merupakan salah satu tanaman yang sudah banyak dimanfaatkan untuk pengobatan manusia karena memiliki kandungan senyawa metabolit sekunder yang berkhasiat obat. Kandungan-kandungan tersebut seperti flavonoid, tannin, dan saponin. Selain untuk pengobatan manusia, daun sambung nyawa juga berpotensi untuk digunakan sebagai obat ikan dalam rangka pencegahan penyakit. Vibriosis merupakan salah satu penyakit bakterial yang disebabkan oleh Vibrio alginolyticus dan rentan menyerang ikan kerapu macan. Penggunaan antibiotik sintetik telah banyak digunakan tetapi menimbulkan banyak dampak buruk, sehingga perlu alternatif baru untuk pencegahan vibriosis. Salah satunya yaitu dengan penggunaan ekstrak daun sambung nyawa, hal ini didukung karena potensinya. Penelitian ini bertujuan untuk mengkaji pengaruh ekstrak daun sambung nyawa untuk meningkatkan ketahanan tubuh ikan kerapu macan sehingga dapat mencegah serangan bakteri Vibrio alginolyticus. Metode yang dilakukan meliputi ekstraksi bahan, uji fitofarmaka, uji in vitro, uji in vivo, uji hematologi, dan uji histopatologi. Dosis ekstrak daun sambung nyawa yang paling efektif untuk meningkatkan ketahanan tubuh ikan kerapu macan dan mencegah serangan Vibrio alginolyticus adalah dosis 700 ppm.</p><p>Kata kunci : daun sambung nyawa, pencegahan, vibriosi</p>

AVALIAÇÃO IN VITRO DO EXTRATO HIDROALCOÓLICO E AQUOSO DE GENGIBRE (Zingiber officinale [WILLD.] ROSCOE) SOBRE O FUNGO Lasiodiplodia theobromae

2018 ◽  
pp. 80
Author(s):  
Lavinia dos Santos Mascarenhas ◽  
Monica Junqueira Machado ◽  
Noelma de Brito Miranda ◽  
Vânia de Jesus Santos Oliveira ◽  
Pedro Henrique Ribeiro Santana
Keyword(s):  
Zingiber Officinale ◽  
Lasiodiplodia Theobromae

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

Antioxidant and Immunomodulatory Activities In Vitro of a Neutral Polysaccharide from Ginger ( Zingiber Officinale)

2021 ◽  
pp. 2100048
Author(s):  
Hou Mingda ◽  
Gao Jun ◽  
Liu Ziqi ◽  
Shan Yu ◽  
Liu Shuyong
Keyword(s):  
Zingiber Officinale

In Vitro Selection of Oligonucleotide Acid Aptamers against Pathogenic Vibrio Alginolyticus by SELEX

2010 ◽  
Vol 439-440 ◽  
pp. 1456-1462 ◽  
Author(s):  
Jiang Zheng ◽  
Yu Bao Li ◽  
Jia Xiang Li ◽  
Jun Wang ◽  
Yong Quan Su
Keyword(s):  
Secondary Structures ◽  
Magnetic Bead ◽  
Vibrio Alginolyticus ◽  
Pathogenic Microorganism ◽  
Asymmetric Pcr ◽  
High Affinity ◽  
Amplification Method ◽  
Pathogenic Vibrio ◽  
Selection Of

Detection of pathogenic microorganism is very necessary in the control of infectious disease prevailing in aquiculture animals. However, most of the present techniques can not meet the need of the quick field inspection. Systematic evolution of ligands by exponential enrichment (SELEX) is a new molecular recognition way for generating high affinity oligonucleotide acid aptamers, a new nucleotide acid material, which have been widely used in the detections of proteins, cells and so on. In the present paper, the technology was applied to select the high affinity aptamers against pathogenic microorganism Vibrio alginolyticus, which could be used for the rapid field detection of the microorganism. Based on the designment of the ssDNA library of 76 nucleotide acids with 35-base random region, the SELEX system for the selection of the high affinity aptamers against Vibrio alginolyticus was established. In the SELEX system, asymmetric PCR was proved to be a better amplification method for the ssDNA library than the reported affinity magnetic bead method, and the corresponding parameters of the asymmetric PCR were also studied and optimized. The affinity of the final ssDNA library increased by nearly 200% compared with the original library. Cloning and sequencing of the final ssDNA library showed that there were at least two kinds of ssDNAs with different length in the affinity ssDNA library: one was 76 bases, another was 149 bases. Simulation of the secondary structures showed that the secondary structures of the two fragments were different greatly, suggesting that the two fragments could bind to different sites of V. alginolyticus surface.

Anti-giardial therapeutic potential of dichloromethane extracts of Zingiber officinale and Curcuma longa in vitro and in vivo

2016 ◽  
Vol 115 (7) ◽  
pp. 2637-2645 ◽  
Author(s):  
Ahmad K. Dyab ◽  
Doaa A. Yones ◽  
Zedan Z. Ibraheim ◽  
Tasneem M. Hassan
Keyword(s):  
Therapeutic Potential ◽  
Curcuma Longa ◽  
Zingiber Officinale

scholarly journals Influence of Zingiber officinale Extract on Push-Out Bond Strength of Glass-Fiber Post

2018 ◽  
Vol 29 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Tânia Mara da Silva ◽  
Virgílio Vilas Boas Fernandes Junior ◽  
Rosemary Soares de Santana ◽  
Renata Marques de Melo Marinho ◽  
Márcia Carneiro Valera ◽  
...  
Keyword(s):  
Bond Strength ◽  
Glass Fiber ◽  
Zingiber Officinale ◽  
Resin Cement ◽  
Fiber Post ◽  
Ginger Extract ◽  
Etch And Rinse ◽  
Glass Fiber Post ◽  
Push Out

Abstract The aim of this study was to evaluate in vitro the influence of 20% glycolic ginger extract on the bond strength of glass-fiber post cemented with etch-and-rinse or self-etching resin cement. Forty-eight bovine roots were standardized (17±0.5 mm) and randomly divided into two groups, according to irrigant used during biomechanical preparation: NaOCl: 1% sodium hypochlorite and GEN: 20% glycolic ginger extract. Root canal was prepared and randomly assigned to one of two subgroups (n=12), according to luting protocol: self-etching (RelyX U200) and etch-and-rinse (RelyX ARC). After 48 h, the roots were sectioned perpendicularly (4 in cervical third and 3 in middle third) and submitted to push-out bond strength test (50 kgf load cell, 1.0 mm/min). Failure mode was analyzed in SEM and stereomicroscopy. Data were analyzed by three-way ANOVA and Tukey tests (p<0.05). ANOVA revealed that there were no significant differences between U200 (2.01±0.17)B and ARC (1.93±0.12)B in GEN group, and at the cervical third for both irrigants, 2.69±0.16A and 2.54±0.17A, respectively. Middle third showed lower bond strength than cervical third, regardless the irrigant. Overall, the cervical third regardless the irrigant employed and the association between NaOCl and ARC, presented better adhesive performance.

In-vitro and in vivo studies of traditionally prepared Zingiber officinale juice for Antimitotic and Lethality activity

2021 ◽  
pp. 4652-4656
Author(s):  
Nadar Sowmya ◽  
Chouhan Raghavendra Singh ◽  
Kosha Patel ◽  
Harshil Patel ◽  
Tanvi Dodiya ◽  
...  
Keyword(s):  
Zingiber Officinale ◽  
In Vivo Studies ◽  
Root Tip ◽  
Inhibition Assay ◽  
Lc50 Value ◽  
Antimitotic Activity ◽  
Ic50 Value ◽  
Traditionally Prepared

According to the literature survey most of the studies done on Adarak (Zingiber officinale rhizome) were performed on alcoholic extracts or isolated entities of ginger but no profound work has been done on the traditionally prepared or commonly consumed way of ginger. So, in this current study, fresh ginger rhizomes were traditionally pounded to make Adarak juice. The different concentration (0.05%, 0.5%, 1%, 2.5%, 5%, 10%) were taken as sample to perform onion root tip inhibition assay and brine shrimp lethality bioassay. It showed antimitotic inhibition in the range of 0.05-10% concentration with an IC50 value of 0.37 % on number of rootlets was 0.08 % on length of rootlets. The traditionally prepared Adarak juice showed cytotoxicity in the range of 0.05-2.5% concentration with LC50 value of 1.59%. The traditionally prepared Adarak juice possesses cytotoxic and antimitotic activity.

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