scholarly journals Factors associated with intramammary infection in dairy cows caused by coagulase-negative staphylococci, Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, Corynebacterium bovis, or Escherichia coli

2017 ◽  
Vol 100 (1) ◽  
pp. 493-503 ◽  
Author(s):  
S. Taponen ◽  
E. Liski ◽  
A.-M. Heikkilä ◽  
S. Pyörälä
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Streptococcus Dysgalactiae ◽  
Coagulase Negative Staphylococci ◽  
Intramammary Infection ◽  
Factors Associated ◽  
Streptococcus Uberis

scholarly journals Subclinical mastitis in dairy cows: somatic cell counts and associated bacteria in Mymensingh, Bangladesh

2017 ◽  
Vol 15 (2) ◽  
Author(s):  
SMMR Sumon ◽  
MA Ehsan ◽  
MT Islam
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Mixed Infections ◽  
Coagulase Negative Staphylococci ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
Bacillus Spp

Subclinical mastitis is an economically important disease of dairy cows and has a prominent place amongst the factors that limit milk production. This study was undertaken to determine the association of somatic cell counts (SCC) and occurrence of bacteria with SCM in smallholder dairy cows in Mymensingh, Bangladesh. A total number of 240 quarters milk samples from apparently healthy lactating cows were subjected to SCC using NucleoCounter® SCC-100 ™ (Chemo Metec). A quarter was considered SCM positive if the quarter had SCC>100 x 103 cells/ml. All subclinical mastitis positive quarter milk samples were subjected to bacteriological examination and isolates were classified into major, minor, uncommon and mixed pathogens. The overall quarter-level prevalence of subclinical mastitis of dairy cows in Mymensingh district was 25% (95% CI, 19.52% to 30.48%). The most frequently isolated bacterial species were Staphylococcus aureus (18.33%) followed by coagulase-negative staphylococci (10%), Enterobacter spp. (6.67%), Escherichia coli (5%), Bacillus spp. (5%) and Pseudomonas aeruginosa (5%). Different bacterial isolates were associated with 90% cases of subclinical mastitis as mono infections or mixed infections. Mono and mixed infections significantly influenced SCC and were the most prominent factors responsible for increasing SCC. Mean SCC was the highest for Bacillus spp. (713.67 x 103 cells/ml) followed by Enterobacter spp. (395.75 x 103 cells/ml), Escherichia coli (386.00 x 103 cells/ml), Staphylococcus aureus (373.82 x 103 cells/ml), coagulase-negative staphylococci (182.67 x 103 cells/ml) and Pseudomonas aeruginosa (138.67 x 103 cells/ml). Major pathogens induced higher SCC (380.72 x 103cells/ml) than minor and other pathogen groups.J. Bangladesh Agril. Univ. 15(2): 266-271, December 2017

scholarly journals Prévalence des principales bactéries responsables de mammites subcliniques des vaches laitières au nord-est de l’Algérie

2012 ◽  
Vol 65 (1-2) ◽  
pp. 5 ◽  
Author(s):  
Z. Boufaida Asnoune ◽  
M. J. Butel ◽  
R. Ouzrout
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Streptococcus Dysgalactiae ◽  
California Mastitis Test ◽  
Streptococcus Uberis

Des analyses bactériologiques de 235 prélèvements de lait de vaches laitières atteintes de mammites subcliniques dépistées par le California mastitis test, provenant de dix exploitations du nord-est de l’Algérie, ont été réalisées  sur une période de deux ans (2008-10). L’isolement bactérien a concerné 89 p. 100 des échantillons. Parmi les germes isolés, les coques à Gram positif ont été les plus fréquents, notamment des staphylocoques à coagulase négative (43 p. 100) et Staphylococcus aureus (30 p. 100). Escherichia coli a été isolé dans 14 p. 100 des prélèvements. Les autres germes isolés ont été Streptococcus uberis (4 p. 100), Streptococcus agalactiae (3 p. 100), Streptococcus dysgalactiae (3 p. 100), et Klebsiella sp. plus rarement (2 p. 100).

scholarly journals Celularidade do leite e Unidades Formadoras de Colônias nas mastites causadas por Staphylococcus coagulase positiva e coagulase negativa

2015 ◽  
Vol 35 (6) ◽  
pp. 518-524 ◽  
Author(s):  
Helio Langoni ◽  
Felipe Freitas Guimarães ◽  
Elizabeth Oliveira da Costa ◽  
Samea Fernandes Joaquim ◽  
Benedito Donizete Menozzi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Streptococcus Agalactiae ◽  
Klebsiella Oxytoca ◽  
Sao Paulo ◽  
São Paulo ◽  
Streptococcus Dysgalactiae ◽  
Streptococcus Uberis

Resumo: A mastite é a principal afecção do gado destinado à produção leiteira, que impacta significativamente a cadeia produtiva do leite, com reflexos ainda para a saúde pública. Estudou-se aspectos relacionados à etiologia, celularidade e de contagem bacteriana em 10 propriedades leiteiras, localizadas no Estado de São Paulo. Foram examinadas 1148 vacas em lactação, totalizando 4584 glândulas mamárias. Foram considerados os casos, em que houve isolamento de estafilococos coagulase positiva (SCP) e estafilococos coagulase negativa (SCN). Os resultados revelaram microbiota com vários patógenos e diferentes espécies de SCN (128 casos) e SCP (45), Staphylococcus aureus(90), Streptococcus agalactiae(70), Streptococcus dysgalactiae (69), Streptococcus uberis(29), Corynebacteriumspp. (230), Klebsiella pneumoniae(28), Klebsiella oxytoca(2), Escherichia coli(15), Enterobactersp. (3). Os resultados de contagem de células somáticas (CCS) relacionados aos SCP e SCN não mostraram diferenças entre as propriedades avaliadas, entretanto com diferenças significantes ao se avaliar a CCS entre os dois grupos de estafilococos, como pode ser evidenciado ao comparar SCN Discreto e SCP exuberante (P<0,01), SCP Discreto e SCP exuberante (P<0,001) e SCN moderado e SCP exuberante (P<0,01). A avaliação da CCS relacionada à intensidade da infecção, considerando-se como crescimento discreto o isolamento de até nove colônias, moderado de dez a 29 colônias e exuberante, com 30 ou mais colônias, revelou para ambos os grupos de estafilococos que quanto maior o número de unidades formadoras de colônias (UFC), a CCS é mais elevada, sendo sempre maior nos casos de SCP. Conclui-se que quando há maior número de UFC, há concomitantemente maior CCS/mL de leite, no caso dos SCP e SCN, o que mostra relação direta da intensidade do processo infeccioso com a resposta da celularidade do leite, bem como pela relevância desses na etiologia das mastites e dos aspectos negativos tanto para a produção, quanto na qualidade do leite produzido nas propriedades.

scholarly journals Possibility of application of dodicin hydrochloride in prevention of mastitis in cows

2006 ◽  
Vol 60 (3-4) ◽  
pp. 207-221
Author(s):  
Svetlana Joksovic ◽  
Vitomir Cupic ◽  
Vera Katic
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Control Group ◽  
Main Approach ◽  
Laboratory Conditions ◽  
Streptococcus Uberis ◽  
Holstein Friesian ◽  
Experimental Group ◽  
Outer Environment

The main approach to curbing mastitis is to prevent the entry of microorganisms from the outer environment into the mammary gland, which is achieved by the use of papilla disinfection following every time of milking. The objective of this work was to examine the antimicrobial activity of the disinfectant dodicin hydrochloride, in fact the preparation that contains this disinfectant (DESU? M), against bacteria, the most frequent causes of mastitis in cows. The efficacy of modified DESU? Mwas examined under laboratory conditions using the quantitative test against the following microorganisms: Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis, under the conditions in the field. The udders of an experimental group of 20 cows of the Holstein-Friesian breed were immersed in a solution of the modified preparation DESU ? M following every time of milking over a period of three months. In the second group of 10 cows of the Holstein-Friesian breed, marked as the control group, no disinfection was applied following the milkings. The preparation DESU? Mexhibited satisfactory antimicrobial efficacy against the most frequent causes (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis) of mastitis in cows under laboratory conditions. With the application of the preparation DESU? M, following every time of milking over a period of three months, the number of somatic cells was reduced by almost one half in comparison with their number at the start of the experiment. No residue of the modified preparation DESU ? M were found in any sample of milk from the experimental group of cows.

scholarly journals Microbiological Study of Diabetic Foot Ulcer

2019 ◽  
Vol 10 (1) ◽  
pp. 50-55
Author(s):  
Mohammuddunnobi ◽  
Tasnuva Jahan ◽  
Abdullah Al Amin
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Klebsiella Pneumonia ◽  
Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening diabetic infections are usually polymicrobial involving multiple aerobic and anaerobic organisms. Methodology: The present study was a cross sectional study, conducted in the department of surgery and microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September' 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is 'any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus'. Results: Majority 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillin-clavulanic acid, amikacin, piperacillin-tazobactam. In Coagulasenegative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem. Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Escherichia coli and Citrobacter spp. Anwer Khan Modern Medical College Journal Vol. 10, No. 1: Jan 2019, P 50-55

scholarly journals Effect of cooling during the dry period on immune response after Streptococcus uberis intramammary infection challenge of dairy cows

2014 ◽  
Vol 97 (12) ◽  
pp. 7426-7436 ◽  
Author(s):  
I.M.T. Thompson ◽  
S. Tao ◽  
A.P.A. Monteiro ◽  
K.C. Jeong ◽  
G.E. Dahl
Keyword(s):  
Immune Response ◽  
Dairy Cows ◽  
Dry Period ◽  
Intramammary Infection ◽  
Streptococcus Uberis
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