Procollagen Type I C-terminal propeptide, procollagen Type III N-terminal propeptide, hyaluronic acid, and laminin in the cerebrospinal fluid of rats with communicating hydrocephalus

2013 ◽  
Vol 11 (6) ◽  
pp. 692-696 ◽  
Author(s):  
Hao Xu ◽  
Zhanxiang Wang ◽  
Shaolin Zhang ◽  
Guowei Tan ◽  
Hongwei Zhu
Keyword(s):  
Hyaluronic Acid ◽  
Saline Control ◽  
Control Group ◽  
Communicating Hydrocephalus ◽  
Type I ◽  
Type Iii ◽  
Procollagen Type ◽  
Procollagen Type I ◽  
Group 5 ◽  
Diagnostic Index

Object Fibrosis along the route of CSF flow is indicated by the development of hydrocephalus. The changes of fibrosis index might reflect the level of hydrocephalus and even become a diagnostic index of hydrocephalus. The object of this study was to analyze the levels of procollagen Type I C-terminal propeptide (PICP), procollagen Type III N-terminal propeptide (PIIINP), hyaluronic acid (HA), and laminin (LN) and their significance in the CSF of communicating hydrocephalus rat models. Methods Thirty adult Sprague-Dawley rats were randomly divided into 3 groups: hydrocephalus group (20 rats) with intraventricular kaolin injections, sham control group (5 rats) with saline injections, and normal group (5 rats) without any processing. The levels of PICP, PIIINP, HA, and LN in the CSF were detected using ELISA. Results Levels of PICP, PIIINP, HA, and LN in the hydrocephalus group were significantly higher than those in the saline control group (p < 0.05). It was revealed by correlation analysis that the increase was positively correlated with the severity of ventricular dilation. Conclusions Results indicated that PICP, PIIINP, HA, and LN continue to rise dramatically in experimental hydrocephalus and may serve as the diagnostic index of hydrocephalus.

scholarly journals Mutations in COL1A1 and COL1A2 Genes Associated with Osteogenesis Imperfecta (OI) Types I or III.

2018 ◽  
Vol 65 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Aleksandra Augusciak-Duma ◽  
Joanna Witecka ◽  
Aleksander L. Sieroń ◽  
Magdalena Janeczko ◽  
Jacek J Pietrzyk ◽  
...  
Keyword(s):  
Osteogenesis Imperfecta ◽  
Collagen Type ◽  
Collagen Type I ◽  
Base Substitution ◽  
Type I ◽  
Intracellular Accumulation ◽  
Single Base ◽  
Type Iii ◽  
Procollagen Type ◽  
Procollagen Type I

Over 85% of osteogenesis imperfecta (OI) cases associates to mutations in procollagen type I genes (COL1A1 or COL1A2), however, no hot spots were linked to particular clinical phenotypes. The 8 patients whom were clinically diagnosed with OI are from Polish population with no ethnic background indicated. Six unpublished mutations were detected in eight patients diagnosed with OI. Genotypes for polymorphisms (Sp1 - rs1800012 and PvuII - rs412777), linked to bone formation and metabolism were also determined. In COL1A1 gene the mutations were found in exons 2, 22, 50 and in introns 13 and 51. In COL1A2 one mutation was identified in exon 22. Mutations of deletion type in COL1A1 that resulted in OI type I an effect neither on collagen type I secretion nor its intracellular accumulation were detected. Also, a single base substitution in I13 (c.904-9 G>T) was associated with OI type I. The OI type III was associated with single base change in I51 of COL1A1, possibly causing an exon skipping. Also, a missense mutation in COL1A2 changing Gly®Cys in the central part of triple helical domain of the collagen type I molecule caused OI type III. It affected secretion of heterotrimeric form of procollagen type I. However, no intracellular accumulation of procollagen chains could be detected. Mutation in COL1A2 affected its incorporation to procollagen type I. The results shall help in genetic counseling of OI patients and provide rational support in making by them and their families conscious, life important decisions.

Increased serum concentrations of type I procollagen C-terminal propeptide and osteocalcin during a short course of calcitriol administration to adult male volunteers

1991 ◽  
Vol 125 (6) ◽  
pp. 609-613 ◽  
Author(s):  
Jeppe Gram ◽  
Jens Bollerslev ◽  
Henning K. Nielsen ◽  
Peter Junker
Keyword(s):  
Serum Concentration ◽  
Serum Levels ◽  
Bone Collagen ◽  
Type I ◽  
Type Iii ◽  
Procollagen Type ◽  
Short Course ◽  
Procollagen Type I ◽  
Serum Hyaluronan ◽  
Healthy Males

Abstract. To investigate bone collagen metabolism during vitamin D treatment, 15 healthy males (aged 28-45 years, median 34) were treated orally with calcitriol, 2 μg daily for 7 days and followed for a total of 2 weeks. The serum concentration of calcitriol rose markedly (median difference and 95% confidence limits: 49% (5-82), p<0.005) during treatment, whereas serum levels of calcidiol, and calcium remained unchanged. The serum level of procollagen type I C-terminal propeptide rose 15% (7-33, p<0.003), whereas no alterations were observed concerning serum procollagen type III N-terminal propeptide, and serum hyaluronan. The serum concentration of osteocalcin rose concomitantly (26% (12-45), p<0.003). All values returned to baseline levels within seven days after the treatment week. The serum levels of osteocalcin and procollagen type I C-terminal propeptide were positively correlated (rs=0.71, p<0.004) during the study. Serum procollagen type I C-terminal propeptide and serum osteocalcin did not correlate with serum procollagen type III N-terminal propeptide or serum hyaluronan either at baseline or after treatment. It is concluded that a short course of calcitriol administration to healthy males stimulates the biosynthesis of bone-related matrix proteins. By contrast, connective tissue components of predominantly extraosseous origin are not affected.

scholarly journals Prevention of osteoporosis with the use of the product of specialized medical nutrition

2016 ◽  
Vol 97 (5) ◽  
pp. 727-731
Author(s):  
S N Beniova ◽  
P F Kiku ◽  
B I Gel’tser ◽  
M V Bobyleva ◽  
E V Korableva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Matrix Protein ◽  
Well Being ◽  
Biologically Active ◽  
Control Group ◽  
Study Group ◽  
Type I ◽  
Procollagen Type ◽  
Procollagen Type I ◽  
Medical Nutrition

Aim. To study the efficacy of shellfish dry extract with «Calcium-reparative» filler as a product of specialized medical nutrition to optimize bone metabolism.Methods. Total of 44 female patients aged from 49 to 71 years, mean age of 54.5±3.8 years, with a combination of several osteoporosis risk factors were involved. 72.7% of all patients complained of pain of varying severity in major joints, in the thoracic and lumbar spine. The patients were randomly divided into two groups - study group (29 patients) and control group (15 patients). Within 30 days, the participants of the study group received «Calcium-reparative» - a product created on the basis of natural biologically active compounds isolated from Far Eastern marine shellfish and purified Altai Shilajit. The efficacy of the product was assessed by means of standard questionnaires for the assessment of quality of life, 10-point visual analogue scale, biochemical laboratory tests. Measurement of serum osteocalcin was performed by ELISA, and immunochemical method - N-terminal propeptide of procollagen type I (the biomarker of bone tissue metabolism).Results. Adverse and allergic reactions to the product were not registered. Among those receiving the product 37.9% noted improvement of well-being and reduction of pain intensity from 6.9±0.45 to 4.28±0.74 points. In those receiving «Calcium-reparative» the level of synthesis of osteocalcin in the study group did not statistically significantly change and appeared to be 18.6±3.8 ng/ml. The increase in the concentration of procollagen type I from 20.9±3.0 ng/ml prior to administration of the therapeutic product to 37.6±5.8 ng/ml (t=0.006, p ≤0.001) after its administration was observed.Conclusion. Product of medical nutrition «Calcium-reparative» has the ability to stimulate the cells of bone tissue to synthesize bone matrix protein collagen type I.

Expression of TGF-β and procollagen type I and type III in human gastric carcinomas

1989 ◽  
Vol 44 (3) ◽  
pp. 394-398 ◽  
Author(s):  
Kazuhiro Yoshida ◽  
Hiroshi Yokozaki ◽  
Minoru Niimoto ◽  
Hisao Ito ◽  
Masanori Ito ◽  
...  
Keyword(s):  
Type I ◽  
Gastric Carcinomas ◽  
Type Iii ◽  
Procollagen Type ◽  
Procollagen Type I

scholarly journals Association between col1a2 Polymorphism and the Occurrence of Pelvic Organ Prolapse in Brazilian Women

2019 ◽  
Vol 41 (01) ◽  
pp. 031-036
Author(s):  
Josyandra Rosa ◽  
Raphael Haddad ◽  
Fabiana Maeda ◽  
Ricardo Souto ◽  
Cesar Fernandes ◽  
...  
Keyword(s):  
Pelvic Organ Prolapse ◽  
Pelvic Organ ◽  
Control Group ◽  
Case Group ◽  
Type I ◽  
Procollagen Type ◽  
Pcr Products ◽  
Procollagen Type I ◽  
Col1a2 Gene ◽  
Polymerase Chain

Objective To evaluate the rs42524 polymorphism of the procollagen type I alpha (α) 2 (COL1A2) gene as a factor related to the development of pelvic organ prolapse (POP) in Brazilian women. Methods The present study involved 112 women with POP stages III and IV (case group) and 180 women with POP stages zero and I (control group). Other clinical data were obtained by interviewing the patients about their medical history, and blood was also collected from the volunteers for the extraction of genomic DNA. The promoter region of the COL1A2 gene containing the rs42524 polymorphism was amplified, and the discrimination between the G and C variants was performed by digestion of the polymerase chain reaction (PCR) products with the MspA1I enzyme followed by agarose gel electrophoresis analysis. Results A total of 292 women were analyzed. In the case group, 71 had the G/G genotype, 33 had the G/C genotype, and 7 had the C/C genotype. In turn, the ratio in the control group was 117 G/G, 51 G/C, and 11 C/C. There were no significant differences between the groups. Conclusion Our data did not show an association between the COL1A2 polymorphism and the occurrence of POP.

Abstract 74: The Inflammatory Phenotype Of Mesenchymal Fibroblasts And Its Role In Aging Dependent Cardiac Fibrosis- A Target For Statins?

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Katarzyna A Cieslik ◽  
JoAnn Trial ◽  
Mark L Entman
Keyword(s):  
Endothelial Cells ◽  
Myeloid Cell ◽  
Transendothelial Migration ◽  
Mouse Heart ◽  
Type I ◽  
Aged Mouse ◽  
Vitro Assay ◽  
Procollagen Type ◽  
Procollagen Type I ◽  
Inflammatory Phenotype

In the aging mouse (C57BL/6) myocardium fibrosis steadily increases after 14 months of age and is accompanied by elevated numbers of myeloid derived fibroblasts. Recently, we proposed a mechanism by which inflammatory mesenchymal fibroblasts (IMF) derived from mesenchymal stem cells secrete monocyte chemoattractant protein-1 (MCP-1) necessary for myeloid fibroblast induction in the aging heart. The current study extends the characterization of this inflammatory phenotype by describing elevated interleukin-6 (IL-6) secretion and increased expression of IL-6 receptor (IL-6R) in IMF. Since IL-6R lacks an intracellular domain it requires a co-receptor gp130 (generally expressed) to induce an intracellular signal. Thus, generation of an IL-6R soluble receptor allows IL-6 signaling on cells that do not express IL-6R (or expression is low), such as endothelial cells. We investigate the function of IL-6 and IL-6R in the promotion of transendothelial migration of monocytes through cardiac endothelium and their maturation into myeloid fibroblasts in in vitro assay. Treatments with IL-6 and more extensively IL-6+IL-6R resulted in a 3-5 fold increase (above the control level) in myeloid cell migration and maturation into myeloid fibroblasts. Thus IMF can contribute both IL-6 and IL-6R to endothelial cells and facilitate myeloid cell transendothelial migration. In agreement with these data, analysis of the aged mouse heart revealed the presence of fibroblasts expressing IL-6 (procollagen type I + IL-6 + cells), M1 macrophages (CD86 + cells) and M2 macrophages (CD301 + procollagen type I + cells) that were absent in hearts from young mice. The mechanisms by which expression of these factors is upregulated in IMF are being investigated; our data suggest that MCP-1 and IL-6 expression are controlled by the farnesyltransferase (FTase)-Ras-Erk1/2 pathway. Interestingly, since atorvastatin interferes with farnesyl synthesis it also reduced MCP-1 and IL-6 expression in IMF. These data may introduce a new use of this class of drugs in the prevention of the age-related fibrosis.

Histologic Characterization of Rat Vocal Fold Scarring

2005 ◽  
Vol 114 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Tomoko Tateya ◽  
Jin Ho Sohn ◽  
Ichiro Tateya ◽  
Diane M. Bless
Keyword(s):  
Hyaluronic Acid ◽  
Vocal Fold ◽  
Type I Collagen ◽  
Collagen Type ◽  
Control Level ◽  
Vocal Folds ◽  
Collagen Type I ◽  
Type I ◽  
Type Iii ◽  
Blue Stain

This study aimed to clarify the characteristics of rat vocal fold scarring by examining the alteration of key components in the extracellular matrix: hyaluronic acid, collagen, and fibronectin. Under monitoring with a 1.9-mm-diameter telescope, unilateral vocal fold stripping was performed, and larynges were harvested at 2, 4, 8, and 12 weeks after operation. The vocal folds were histologically analyzed with Alcian blue stain, trichrome stain, and immunofluorescence of collagen type I, collagen type III, and fibronectin. The scarred vocal folds showed less hyaluronic acid and more collagen types I and III than did the controls at all time points. Type III was stable for 12 weeks, while type I declined until 8 weeks and thereafter remained unchanged. Fibronectin increased for 4 weeks and then decreased; it was close to the control level at 8 and 12 weeks. These results suggest that the tissue remodeling process in scarred vocal folds slows down around 2 months after wounding.

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