THE EFFECTS OF EARLY POSTOPERATIVE IMMUNIZATION WITH XENOGENEIC EMBRYO PROTEINS  ON LEWIS LUNG CARCINOMA MODEL

Aim: To investigate the effect of chicken embryo proteins (CEP) as a prototype of xenogeneic vaccine on immune reactions in mice immunized after Lewis lung carcinoma (LLC) surgical removal. Materials and Methods: C57Bl male mice were immunized on days 1, 8, and 15 after surgical removal of LLC. The immune response was assessed on days 7, 14, 21 and 28 after tumor resection. Cytotoxic activity of natural killer cells (NK) and cytotoxic T-lymphocytes as well as antibody dependent cellular cytotoxicity was estimated in MTT-assay; specific antibodies were detected in ELISA; lymphocyte proliferation was tested in reaction of in vitro blast transformation. Results: None of the immunized mice developed LLC metastases. Immunization with CEP seems to prevent the potential decrease in NK cell cytotoxic activity and spontaneous blast transformation activity of lymphocytes following the surgically induced stress. Further research on improving immunization schedule and elucidating the mechanisms of NK modulation with CEP is needed.

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Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALB/c Mice

Advances in Materials Science and Engineering ◽

10.1155/2019/3534807 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Thi Thao Do ◽

Thi Phuong Do ◽

Thi Nga Nguyen ◽

Thi Cuc Nguyen ◽

Thi Thu Phuong Vu ◽

...

Keyword(s):

Side Effects ◽

Antitumor Activity ◽

Cytotoxic Activity ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lewis Lung ◽

Antitumor Activities ◽

Antitumor Effects ◽

Carcinoma Tumor

Although L-Asparaginase (L-ASP) is an effective chemotherapeutic agent, it has side effects such as fever, skin rashes, chills, anaphylaxis, and severe allergic reactions. Moreover, the short half-life of L-ASP reduces its antitumor activity. To reduce its side effects and broaden its pharmaceutical applications, L-ASP obtained from Pectobacterium carotovorum was subjected to liposomal conjugation. The enzyme was then loaded into liposomes using the hydrated thin-film method. The in vitro cytotoxic activity of liposomal L-ASP was evaluated with the MTT assay using cancerous cell lines, and its antitumor effects were examined in Lewis lung carcinoma (LLC) tumorized mice. The average size of the liposomes containing purified L-asparagine was 93.03 ± 0.49 nm. They had a zeta potential of –15.45 ± 6.72 mV, polydispersity index of 0.22 ± 0.02, and encapsulation efficiency of 53.99 ± 5.44%. The in vitro cytotoxic activity of liposomal L-ASP was less effective against LLC, MCF-7 (human breast carcinoma), HepG2 (human hepatocellular carcinoma), SK-LU-1 (human lung carcinoma), and NTERA-2 (pluripotent human embryonic carcinoma) cells than that of free L-ASP. However, the antitumor activity of liposomal L-ASP was significantly greater than that of untrapped L-ASP at the same doses (6 UI/mouse) in terms of tumor size (6309.11 ± 414.06 mm3) and life span (35.00 ± 1.12 days). This is the first time the antitumor activities of PEGylated nanoliposomal L-ASP have been assessed in LLC carcinoma tumor-induced BALB/c mice and showed significantly improved pharmacological properties compared to those of free L-ASP (P<0.05). Thus, nanoliposomal L-ASP should be considered for its widening applications against carcinoma tumors.

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Asian ginseng extract inhibits in vitro and in vivo growth of mouse lewis lung carcinoma via modulation of ERK-p53 and NF-κB signaling

Journal of Cellular Biochemistry ◽

10.1002/jcb.22778 ◽

2010 ◽

Vol 111 (4) ◽

pp. 899-910 ◽

Cited By ~ 38

Author(s):

Vincent Kam Wai Wong ◽

Simon Shiu Fai Cheung ◽

Ting Li ◽

Zhi-Hong Jiang ◽

Jing-Rong Wang ◽

...

Keyword(s):

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lewis Lung ◽

Ginseng Extract ◽

In Vivo Growth

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Relationship of cell cycle parameters to in vitro and in vivo chemosensitivity for a series of Lewis lung carcinoma lines

European Journal of Cancer ◽

10.1016/0959-8049(92)90537-c ◽

1992 ◽

Vol 28 (8-9) ◽

pp. 1427-1431 ◽

Cited By ~ 13

Author(s):

Karen M. Holdaway ◽

Graeme J. Finlay ◽

Bruce C. Baguley

Keyword(s):

Cell Cycle ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lewis Lung ◽

Relationship Of

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Effect of Micelle-Incorporated Cisplatin With Sizes Ranging From 8 to 40 nm for the Therapy of Lewis Lung Carcinoma

Frontiers in Pharmacology ◽

10.3389/fphar.2021.632877 ◽

2021 ◽

Vol 12 ◽

Author(s):

Zhicheng Wang ◽

Yumin Li ◽

Tong Zhang ◽

Hongxia Li ◽

Zhao Yang ◽

...

Keyword(s):

Block Copolymers ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Drug Loading ◽

Complexation Reaction ◽

Lewis Lung ◽

Antitumor Effects ◽

Lung Carcinoma Cells

Insufficient transport of therapeutic cargo into tumor bed is a bottleneck in cancer nanomedicine. Block copolymers are promising carriers with smaller particle size by ratio modification. Here, we constructed cisplatin nanoparticles with sizes ranging from 8 to 40 nm to study the permeability and therapy of Lewis lung carcinoma. We synthesized methoxypoly(ethylene glycol)2000-block poly(L-glutamic acid sodium salt)1979 loading cisplatin through complexation reaction. The cisplatin nanomedicine has high drug loading and encapsulation efficiency. In vitro data demonstrated that cisplatin nanoparticles had equivalent growth-inhibiting effects on Lewis lung carcinoma cells compared to free cisplatin. In vivo evidences showed cisplatin nanoparticles had superior antitumor effects on the Lewis lung carcinoma mouse model with no obvious side effects. All results indicated that optimizing the ratio of block copolymers to obtain smaller sized nanomedicine could act as a promising strategy for overcoming the inadequate accumulation in poorly vascularized tumors.

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Gold Nanoparticles Radio-sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

10.20944/preprints202007.0708.v1 ◽

2020 ◽

Author(s):

Arvind Pandey ◽

Veronica Vighetto ◽

Nicola Di Marzio ◽

Matteo Hirsch ◽

Nicola Ferrante ◽

...

Keyword(s):

Dna Damage ◽

Particle Size ◽

Gold Nanoparticles ◽

Cell Survival ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lewis Lung ◽

Lung Carcinoma Cells ◽

Reduce Cell Survival

It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis Lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After the treatment of 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis Lung carcinoma cells treated with gold nanoparticles.

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