The effect of potassium iodide, methylene blue and other substances applied to the embryo sacs of seed-plants

1914 ◽  
Vol 12 (1) ◽  
pp. 1-3
Author(s):  
D. T. MacDougal
Keyword(s):  
Methylene Blue ◽  
Potassium Iodide ◽  
Seed Plants ◽  
Other Substances

Method for the Quantification of Alginate in Microcapsules

1993 ◽  
Vol 2 (5) ◽  
pp. 429-436 ◽  
Author(s):  
Jean-Pierre Hallé ◽  
Danielle Landry ◽  
Alain Fournier ◽  
Michèle Beaudry ◽  
Francois A. Leblond
Keyword(s):  
Methylene Blue ◽  
Alkaline Medium ◽  
High Sensitivity ◽  
Sodium Concentration ◽  
The Other ◽  
Sensitive Assay ◽  
Reliable Measurement ◽  
Alginate Concentration ◽  
Other Substances ◽  
Alginate Content

Alginate is a key reagent in the preparation of microcapsules for cell transplantation. To address the question of the intracapsular alginate concentration, a sensitive assay has been developed to quantify the alginate content of microcapsules. The method is based on the metachromatic change induced by alginate binding to the dye, 1,9-dimethyl methylene blue (DMMB). The assay has a high sensitivity and precision. It covers a wide concentration range enabling the measurement of alginate in dilute supernatants as well as in microcapsules. For the latter, the membrane is initially dissolved by incubating the microcapsules in an alkaline medium. The effect of potentially interfering substances (poly-l-lysine (PLL), citrate, chloride, sodium) and of pH has been studied. Poly-l-lysine interfered with the assay at pH 6.5 but not at pH 13. Interference by sodium augmented with increasing sodium concentration and reached a plateau at 200 mM. This problem was overcome by routinely adjusting all samples to 500 mM sodium. The other substances tested had a negligible effect on the assay. The reliable measurement of alginate with this new assay will allow the optimization of the intracapsular alginate concentration.

Suction through the vagina

1926 ◽  
Vol 22 (5-6) ◽  
pp. 744
Author(s):  
A. T.
Keyword(s):  
Methylene Blue ◽  
Potassium Iodide

Absorption through the vagina was studied by Robinson (Journ. Of obs. A. Gyn. Of Brit. Emp., 1925, v. 32, No. 3), who investigated solutions of potassium iodide, salicylic sodium, quinine, glucose, phenolrot and methylene blue.

scholarly journals Bacterial Photodynamic Inactivation Mediated by Methylene Blue and Red Light Is Enhanced by Synergistic Effect of Potassium Iodide

2015 ◽  
Vol 59 (9) ◽  
pp. 5203-5212 ◽  
Author(s):  
Daniela Vecchio ◽  
Asheesh Gupta ◽  
Liyi Huang ◽  
Giacomo Landi ◽  
Pinar Avci ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Potassium Iodide ◽  
Bacterial Species ◽  
Red Light ◽  
Multidrug Resistant ◽  
Photodynamic Inactivation ◽  
Bacterial Killing ◽  
Risk Of Death

ABSTRACTThe inexorable increase of antibiotic resistance occurring in different bacterial species is increasing the interest in developing new antimicrobial treatments that will be equally effective against multidrug-resistant strains and will not themselves induce resistance. One of these alternatives may be photodynamic inactivation (PDI), which uses a combination of nontoxic dyes, called photosensitizers (PS), excited by harmless visible light to generate reactive oxygen species (ROS) by type 1 (radical) and type 2 (singlet oxygen) pathways. In this study, we asked whether it was possible to improve the efficacy of PDIin vitroandin vivoby addition of the inert salt potassium iodide (KI) to a commonly investigated PS, the phenothiazinium dye methylene blue (MB). By adding KI, we observed a consistent increase of red light-mediated bacterial killing of Gram-positive and Gram-negative speciesin vitroandin vivo. In vivo, we also observed less bacterial recurrence in wounds in the days posttreatment. The mechanism of action is probably due to formation of reactive iodine species that are produced quickly with a short lifetime. This finding may have a relevant clinical impact by reducing the risk of amputation and, in some cases, the risk of death, leading to improvement in the care of patients affected by localized infections.

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Experts Warn Against Taking Potassium Iodide

Ob Gyn News ◽  
2011 ◽  
Vol 46 (4) ◽  
pp. 2
Author(s):  
SHERRY BOSCHERT
Keyword(s):  
Potassium Iodide
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