Influence of Soybean Phosphatide on Blood Coagulation and its Use in the Thromboplastin Generation Test

1958 ◽  
Vol 97 (1) ◽  
pp. 38-43 ◽  
Author(s):  
W. E. Connor ◽  
J. R. Carter
Keyword(s):  
Blood Coagulation ◽  
Soybean Phosphatide ◽  
Generation Test

scholarly journals Wave propagation for a reduced model of blood coagulation

2020 ◽  
Vol 31 ◽  
pp. 01005
Author(s):  
Nicolas Ratto ◽  
Martine Marion ◽  
Vitaly Volpert
Keyword(s):  
Wave Propagation ◽  
Mathematical Models ◽  
Blood Coagulation ◽  
Thrombin Generation ◽  
Reduced Model ◽  
Generation Test

Reduced mathematical models of blood coagulation can properly describe Thrombin Generation Test (TGT). Clot growth occurs as a reactiondiffusion wave, and the reduced model studied in this work describes this behaviour, preserving the differences observed between healthy and hemophilia subjects in the TGT.

The “Retarded” Thromboplastin-Generation Test: Its Use in the Study of Accelerated Blood Coagulation

1962 ◽  
Vol 37 (1) ◽  
pp. 63-65 ◽  
Author(s):  
John H. Thompson ◽  
Charles A. Owen ◽  
John A. Spittell ◽  
Chris A. Pascuzzi ◽  
Charlene Matthees
Keyword(s):  
Blood Coagulation ◽  
Generation Test

Acute Pancreatitis in Man and Blood Coagulation Disturbances

1979 ◽  
Author(s):  
H. Kõtering ◽  
M. Hasenbein ◽  
H. Artmann ◽  
U. Kasten ◽  
J. Kellermann
Keyword(s):  
Acute Pancreatitis ◽  
Blood Coagulation ◽  
Thrombin Generation ◽  
The Other ◽  
Phospholipase A ◽  
Coagulation System ◽  
Consumption Coagulopathy ◽  
Blood Coagulation System ◽  
Severe Pancreatitis ◽  
Generation Test

The pathogenesis of blood coagulation-disturbances in patients with acute pancreatitis in man is still unknown. Therefore we studied repeatedly the blood coagulation system of all patients with acute pancreatitis, who were admitted to our clinic or were transferred from other hospitals after complications occurred. 19 patients with a severe pancreatitia were studied. Most of them showed oliguria, pancreatic lungs, thrombosis or haemorrhage. Only 9 determinations (in 5 patients) resulted an enhancement of thrombin generation in the Thrombin-Generation-Test (TGT). All the other patients showed already hypocoagulsbility in the TGT and severe signs of DIC and consumption coagulopathy with a loss of platelets, fibrinogen and prothrombin complex. In 9 patients, who died, we found histomorphologicaliy fibrin deposites and hyaline thrombi. In comparison to 58 patients with elevated amylases but no severe pancreatitiS we found, that the initial alteration of blood coagulation system in pancreatitis is a hypercoagulaoility, possibly caused by trypsin, phospholipase A or elastase.

scholarly journals Coagulation status in patients with pulmonary embolism receiving long-term anticoagulant therapy

2022 ◽  
Vol 20 (8) ◽  
pp. 3093
Author(s):  
E. A. Shmidt ◽  
S. A. Berns ◽  
T. Yu. Penskaya ◽  
I. I. Zhidkova ◽  
O. V. Gruzdeva ◽  
...  
Keyword(s):  
Pulmonary Embolism ◽  
Blood Coagulation ◽  
Anticoagulant Therapy ◽  
Thrombin Generation ◽  
Fibrin Clot ◽  
Coagulation Rate ◽  
Integral Methods ◽  
Coagulation Status ◽  
Generation Test

Aim. To study the blood coagulation status by various laboratory methods in patients after pulmonary embolism (PE) receiving long-term anticoagulant therapy.Material and methods. The blood of 23 patients with pulmonary embolism, who received long-term anticoagulant therapy, was studied. The study of coagulation profile, D-dimer, thrombodynamics, thromboelastography and thrombin generation test were carried out.Results. The thrombin generation test shows a significant increase in the time of its formation, while the maximum amount of thrombin formed is half that of the reference values. There is a slightly increased median fibrin clot growth rate in the thrombodynamics test — 30,4 gm/min with a normal coagulation rate of 20-29 gm/min. The result of thromboelastography also reflects the blood hypocoagulation, in terms of R, Angle a and CI.Conclusion. Integral methods for assessing the thrombotic readiness in combination with a routine coagulation panel demonstrate a complete picture of blood coagulation potential in patients after pulmonary embolism requiring long-term anticoagulant therapy.

The Effect of Trasylol on Blood Coagulation after Intravenous Injection

1967 ◽  
Vol 17 (01/02) ◽  
pp. 051-057
Author(s):  
J Gormsen ◽  
P Josephsen
Keyword(s):  
Blood Coagulation ◽  
Intravenous Injection ◽  
Significant Influence ◽  
Partial Thromboplastin Time ◽  
Thrombin Generation ◽  
Double Blind ◽  
Generation Test

SummaryThe in vivo effect of Trasylol® on the thromboplastin generation test, thrombin -generation test, the partial thromboplastin time and the thrombelastography has been examined in 50 patients. Furthermore 34 patients were given one of two preparations of “Trasylol”, distributed for a double-blind control. The dosis of Trasylol varied from 100,000 to 400,000 units. No significant influence on the various tests used was found.

Is There an Enhancing Effect of Neuroleptic Drugs on Blood Coagulation and Thrombosis

1965 ◽  
Vol 13 (02) ◽  
pp. 418-427 ◽  
Author(s):  
T Astrup ◽  
J Rasmussen
Keyword(s):  
Blood Coagulation ◽  
Direct Effect ◽  
Thrombin Generation ◽  
Neuroleptic Drugs ◽  
Secondary Effects ◽  
Heavy Sedation ◽  
Inhibiting Effect ◽  
Normal Human ◽  
Significant Direct Effect ◽  
Generation Test

SummarySordinol (R), (clopenthixol, W.H. 0.), a potent sedative of the thiaxanthene group, in the concentrations used therapeutically, had no direct influence on blood coagulation as investigated by the thrombin generation test and the thromboplastin activation test. No significant direct effect on the fibrinolytic system was observed using urokinase, streptokinase activated human euglobulin, trypsin, porcine plasmin, or human plasmin. There was no effect on the urokinase-inhibiting effect of normal human plasma. The influence of the immobilization produced by heavy sedation on the development of thrombotic states is discussed. When the effects of drugs are evaluated it is necessary to distinguish between the direct effect of the drug and secondary effects caused by the treatment.

PHOSPHOLIPIDS: I. STUDIES ON THE THROMBOPLASTIC AND ANTICLOTTING ACTIVITY OF PHOSPHOLIPIDS

1965 ◽  
Vol 43 (6) ◽  
pp. 781-786 ◽  
Author(s):  
P. J. Grisdale ◽  
A. Okany
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Blood Coagulation ◽  
Preparative Thin Layer Chromatography ◽  
Layer Chromatography ◽  
Generation Test

Preparative thin-layer chromatography, combined with published procedures, has facilitated the isolation of chromatographically homogeneous phosphatidylserine, phosphatidylethanolamine, and lysophosphatidylethanolamine. The effect of these compounds and their mixtures on blood coagulation has been studied in the thromboplastin-generation test. Evidence is produced to demonstrate that the results of such tests for anticlotting activity are dependent on the choice of a standard clotting agent.

scholarly journals Factor VII Deficiency in the Beagle Dog

1968 ◽  
Vol 2 (2) ◽  
pp. 105-112 ◽  
Author(s):  
K. Capel-Edwards ◽  
O. E. Hall
Keyword(s):  
Blood Coagulation ◽  
Factor Vii ◽  
Blood Coagulation Disorders ◽  
Coagulation Disorders ◽  
Beagle Dog ◽  
Physical Evidence ◽  
Factor Vii Deficiency ◽  
One Stage ◽  
Healthy Female ◽  
Generation Test

Two cases are described of factor VII deficiency in the blood of healthy female beagles. The diagnosis was established on the basis of: (i) prolonged Quick's one-stage prothrombin and ‘Thrombotest’ times, both corrected by the addition of 10 per cent by volume of normal canine serum; (ii) normal Russell's viper venom time and thromboplastin generation test; and (iii) reduced factor VII activity compared with plasma from a normal dog. Attention is drawn to some implications of inherited blood-coagulation disorders not associated with physical evidence of bleeding.

The Effect of Fatty-acid Autoxidation Products on Blood Coagulation

1975 ◽  
Vol 33 (02) ◽  
pp. 271-277 ◽  
Author(s):  
T. W Barrowcliffe ◽  
J. M. C Guttteridge ◽  
T. L Dormandy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Blood Coagulation ◽  
Thrombin Generation ◽  
Water Soluble ◽  
Oxidation Products ◽  
Coagulant Activity ◽  
Rate Of Decay ◽  
Generation Test

SummaryPolyunsaturated fatty acids were allowed to autoxidise in air over 4 days. The water soluble oxidation products were extracted at daily intervals and tested for their effect on blood coagulation. After 1 day there was slight acceleration of the recalcification and RVV times, but from 2–4 days the extracts became increasingly inhibitory. The P.T. and P.T.T. were also inhibited. In the thrombin generation test the extracts delayed the appearance of thrombin, but the peak thrombin level was increased and its rate of decay was reduced. When added to phospholipid the extracts altered their coagulant activity. The presence of autoxidation products could account for some of the variable results obtained with different preparations of phospholipids.

Influence of Serum Incubation Conditions on Asolectin Thromboplastin Generation Results

1960 ◽  
Vol 4 (01) ◽  
pp. 083-092
Author(s):  
Arthur J. Seaman ◽  
Karen-Marie Karlsen
Keyword(s):  
Sodium Citrate ◽  
Normal Serum ◽  
Hemophilia B ◽  
Good Separation ◽  
Minimal Time ◽  
Serum Activity ◽  
Normal Populations ◽  
Soybean Phosphatide ◽  
Generation Test ◽  
Oxalated Plasma

SummaryThromboplastin generation in a system employing Asolectin, a soybean phosphatide, suspension as a platelet substitute is greatly influenced by the time and temperature of serum incubation.Reproducible results and good separation of populations of normal and PTC deficient sera were obtained in the asolectin TGT system when test sera were incubated for 1 hour at 20° C prior to the thromboplastin generation test. Longer time or higher temperatures of serum incubation resulted in progressively less reliable, separation of pathologic and normal populations in asolectin TGT systems than in fresh platelet TGT systems. Cephalin suspension was shown in a smaller number of tests to behave similarly to asolectin suspension when used as a platelet substitute in TGT systems.The 0.14 M sodium citrate eluate of citrated normal or Hemophilia B sera (incubated 1 hour at 37° C) had the ability to shorten the prolonged asolectin TGT minimal time of “over-incubated” normal sera to values obtained with the same sera in fresh platelet TGT systems. This correction, however, was probably non-specific since Hemophilia B sera results were sometimes similarly normalized. Citrated (but not oxalated) plasma incubated at 37° C for 24 hours, then recalcified to manufacture serum, and incubated an additional hour at 20° C showed no loss of the fresh 1 hour 20° C serum activity in asolectin thromboplastin generation, further suggesting that the thermolabile component of normal serum influencing asolectin TGT is a reaction product evolved during clotting.

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