Welcome Address 9th International Symposium Europe-China Stem Cells and Regenerative Medicine

Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices.

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Stem cell therapies and benefaction of somatic cell nuclear transfer cloning in COVID-19 era

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02334-5 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Birbal Singh ◽

Gorakh Mal ◽

Vinod Verma ◽

Ruchi Tiwari ◽

Muhammad Imran Khan ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Regenerative Medicine ◽

Somatic Cell ◽

Somatic Cell Nuclear Transfer ◽

Nuclear Transfer ◽

Drug Testing ◽

Human Interaction ◽

Patient Specific ◽

Wide Range

Abstract Background The global health emergency of COVID-19 has necessitated the development of multiple therapeutic modalities including vaccinations, antivirals, anti-inflammatory, and cytoimmunotherapies, etc. COVID-19 patients suffer from damage to various organs and vascular structures, so they present multiple health crises. Mesenchymal stem cells (MSCs) are of interest to treat acute respiratory distress syndrome (ARDS) caused by SARS-CoV-2 infection. Main body Stem cell-based therapies have been verified for prospective benefits in copious preclinical and clinical studies. MSCs confer potential benefits to develop various cell types and organoids for studying virus-human interaction, drug testing, regenerative medicine, and immunomodulatory effects in COVID-19 patients. Apart from paving the ways to augment stem cell research and therapies, somatic cell nuclear transfer (SCNT) holds unique ability for a wide range of health applications such as patient-specific or isogenic cells for regenerative medicine and breeding transgenic animals for biomedical applications. Being a potent cell genome-reprogramming tool, the SCNT has increased prominence of recombinant therapeutics and cellular medicine in the current era of COVID-19. As SCNT is used to generate patient-specific stem cells, it avoids dependence on embryos to obtain stem cells. Conclusions The nuclear transfer cloning, being an ideal tool to generate cloned embryos, and the embryonic stem cells will boost drug testing and cellular medicine in COVID-19.

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A Step Toward Optimizing Regenerative Medicine Principle to Combat COVID-19

Annals of the National Academy of Medical Sciences (India) ◽

10.1055/s-0041-1731597 ◽

2021 ◽

Author(s):

Shilpa Sharma ◽

Madhan Jeyaraman ◽

Sathish Muthu ◽

Talagavadi Channaiah Anudeep ◽

Naveen Jeyaraman ◽

...

Keyword(s):

Clinical Trial ◽

Stem Cells ◽

Regenerative Medicine ◽

Large Volume ◽

Beneficial Role

AbstractDrugs are currently not licensed in specific to pulverize COVID-19. On an emergency basis, vaccines were approved to prevent the further spread of COVID-19. This serves as a potential background for considering the optimization of biologics. In this context, evidence on convalescent plasma and stem cells has shown a beneficial role. Here, we have considered this as plausible therapy, and further hypothesize that their cocktails will synergistically boost the immunogenicity to relegate COVID-19. This warrants a large volume clinical trial on an emergent basis, because the sooner we establish a safe and effective cure, the better.

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Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century

Stem Cells International ◽

10.1155/2015/734731 ◽

2015 ◽

Vol 2015 ◽

pp. 1-19 ◽

Cited By ~ 76

Author(s):

J.-F. Stoltz ◽

N. de Isla ◽

Y. P. Li ◽

D. Bensoussan ◽

L. Zhang ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Regenerative Medicine ◽

Embryonic Stem ◽

Clinical Applications ◽

Cardiac Insufficiency ◽

Immunomodulatory Activity ◽

Hematopoietic Stem ◽

Fetal Stem Cells ◽

Organ Regeneration

Since the 1960s and the therapeutic use of hematopoietic stem cells of bone marrow origin, there has been an increasing interest in the study of undifferentiated progenitors that have the ability to proliferate and differentiate into various tissues. Stem cells (SC) with different potency can be isolated and characterised. Despite the promise of embryonic stem cells, in many cases, adult or even fetal stem cells provide a more interesting approach for clinical applications. It is undeniable that mesenchymal stem cells (MSC) from bone marrow, adipose tissue, or Wharton’s Jelly are of potential interest for clinical applications in regenerative medicine because they are easily available without ethical problems for their uses. During the last 10 years, these multipotent cells have generated considerable interest and have particularly been shown to escape to allogeneic immune response and be capable of immunomodulatory activity. These properties may be of a great interest for regenerative medicine. Different clinical applications are under study (cardiac insufficiency, atherosclerosis, stroke, bone and cartilage deterioration, diabetes, urology, liver, ophthalmology, and organ’s reconstruction). This review focuses mainly on tissue and organ regeneration using SC and in particular MSC.

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