scholarly journals Characterization of a diverse USDA collection of wild soybean (glycine soja siebold and zucc.) accessions and subsequent mapping for seed composition and agronomic traits in a RIL population

2018 ◽  
Author(s):  
◽  
Thang Cao La
Keyword(s):  
Plant Height ◽  
Seed Weight ◽  
Core Collection ◽  
Seed Protein ◽  
Agronomic Traits ◽  
Glycine Soja ◽  
Block Design ◽  
Wild Soybean ◽  
Field Tests ◽  
Plant Introductions

The relatively low genomic variation of current U.S. soybean [Glycine max (L.) Merill] cultivars constrains the improvement of grain yield, seed quality, and other agronomic traits within soybean breeding programs. Recently, a substantial effort has been undertaken to introduce novel genetic diversity present in wild soybean (Glycine soja Siebold and Zucc.) into new elite cultivars, in both public and private applied soybean breeding programs. The objectives of this research were to evaluate the phenotypic diversity within a core collection of 80 G. soja plant introductions (PIs) in the United States Department of Agriculture National Genetic Resources Program that were collected in China, Japan, Russia, and South Korea, and to analyze the correlations between agronomic and seed composition traits. Field tests were conducted in Missouri and North Carolina during three years, 2013, 2014, and 2015, in a randomized complete block design (n=3). The phenotypic data collected included plant maturity date, seed weight, and the seed concentration of protein, oil, essential amino acid, fatty acid, and soluble carbohydrates. Analyzing the data from six environments, we found genotype was a significant (p less than 0.0001) source of variation for maturity date, seed weight, seed protein and amino acids, seed oil and fatty acids, and seed carbohydrates. Significant correlations were observed between numerous traits. The core collection had lower seed weight, higher seed content of protein, linolenic acid, raffinose and stachyose but lower seed content of oil and oleic acid than those of the cultivated soybean lines that were used as checks. The amino acid profile of the core collection was significantly different from that of the checks. An association analysis revealed 19 SNP that were significantly associated with maturity, seed weight, and seed contents of aspartic acid, glutamine, palmitic acid, oleic acid, and linoleic acid. The information and data collected in this study will be invaluable in guiding soybean breeders and geneticists in selecting promising Glycine soja plant introductions for research and cultivar improvement. In addition the identification of quantitative trait loci (QTLs) associated with the contents of seed protein and oil, maturity, branching traits, height, lodging, and yield in a recombinant inbred line (RIL) population developed from one single F2 plant from the cross between Osage and PI593983 was carried out. The mapping population in this study included 164 F4:6 recombinant inbred lines (RILs) derived from a cross between Osage, a cultivated soybean variety, and PI593983, a wild soybean accession. Field tests were carried out in Missouri for two years during 2016 and 2017, in a randomized complete block design (n=2). Both protein and oil contents showed high heritabilities. Seed protein and seed oil were negatively correlated (-0.77). A total of 4,374 polymorphic markers were used to construct a genetic linkage map, and nine QTLs for protein content, explained 7.6 to 36.7% of variance, and seven QTLs for oil content, explained for 7.8 to 29.7% of variance, were detected using composite interval mapping. addition we identified eight novel QTLs and confirmed sixteen QTLs associated with maturity (R2 = 6.4 to 26.3%), plant height (R2 = 7.4 to 15.5%), and total branch length (R2 = 9.3% and 14.5%) in individual and across environments, and the ratio of total branch length to plant height (R2 = 11.8%), yield (R2 =12.8 and 15.7), and lodging (R2 = 12.1 and 13.4) in individual studied environments. Sixteen QTLs for maturity, yield, and plant height confirmed previously reported QTLs, and eight QTLs have not been reported before. The results of this study will facilitate the identification of the causative genes for seed protein and oil, maturity, height, lodging, and branching traits, and will help soybean breeder improve soybean performance by developing markers for marker-assisted selection.

scholarly journals Strategies to select soybean segregating populations with the goal of improving agronomic traits

2018 ◽  
Vol 40 (1) ◽  
pp. 39324
Author(s):  
Fabiana Mota da Silva ◽  
Elise De Matos Pereira ◽  
Bruno Henrique Pedroso Val ◽  
Dilermando Perecin ◽  
Antonio Orlando Di Mauro ◽  
...  
Keyword(s):  
Plant Height ◽  
Genetic Gain ◽  
Seed Weight ◽  
Agronomic Traits ◽  
Block Design ◽  
Selection Strategy ◽  
Breeding Programs ◽  
Effective Selection ◽  
100 Seed Weight ◽  
Number Of Branches

The success of breeding programs depends on selection procedures and on the breeding methods adopted for selecting segregating populations. The objective of this study was to evaluate the efficiency of the Bulk method with selection in the F3 generation (BulkF3) compared to that of Bulk method as well as determine the most effective selection strategy in terms of genetic gain. Twenty segregating populations were selected by two methods. The 60 best families of each method were selected according to their average agronomic performance. An augmented block design was used. The following agronomic traits were evaluated: insertion height of first pod, plant height at maturity, number of branches and of pods per plant, 100-seed weight, and grain yield. For comparison of the methods, genetic component estimates, genetic gain and predicted breeding values were calculated using mixed models (REML and BLUP). The results showed the families obtained with the BulkF3 method were more productive, showed suitable plant height, a larger number of branches and pods, and higher 100-seed weight. The BulkF3 method was found to be an effective selection strategy for soybean improvement. 

scholarly journals Genome-wide association study and genomic selection for plant height, maturity, seed weight, and yield in soybean

2019 ◽  
Author(s):  
Waltram Ravelombola ◽  
Jun Qin ◽  
Ainong Shi ◽  
Fengmin Wang ◽  
Yan Feng ◽  
...  
Keyword(s):  
Association Study ◽  
Genomic Selection ◽  
Candidate Genes ◽  
Plant Height ◽  
Seed Weight ◽  
Genome Wide Association Study ◽  
Agronomic Traits ◽  
Snp Markers ◽  
Genome Wide Association ◽  
Genome Wide

Abstract Background Soybean [ Glycine max (L.) Merr.] is a legume of great interest worldwide. Enhancing genetic gain for agronomic traits via molecular approaches has been long considered as the main task for soybean breeders and geneticists. The objectives of this study were to evaluate maturity, plant height, seed weight, and yield in a diverse soybean accession panel, to conduct a genome-wide association study (GWAS) for these traits and identify SNP markers associated with the four traits, and to assess genomic selection (GS) accuracy. Results A total of 250 soybean accessions were evaluated for maturity, plant height, seed weight, and yield over three years. This panel was genotyped with a total of 10,259 high quality SNPs postulated from genotyping by sequencing (GBS). GWAS was performed using a Bayesian Information and Linkage Disequilibrium Iteratively Nested Keyway (BLINK) model, and GS was evaluated using a ridge regression best linear unbiased predictor (rrBLUP) model. The results revealed that a total of 20, 31, 37, 31, and 23 SNPs were significantly associated with the average 3-year data for maturity, plant height, seed weight, and yield, respectively; some significant SNPs were mapped into previously described loci ( E2 , E4 , and Dt1 ) affecting maturity and plant height in soybean and a new locus mapped on chromosome 20 was significantly associated with plant height; Glyma.10g228900 , Glyma.19g200800 , Glyma.09g196700 , and Glyma.09g038300 were candidate genes found in the vicinity of the top or the second best SNP for maturity, plant height, seed weight, and yield, respectively; a 11.5-Mb region of chromosome 10 was associated with both seed weight and yield; and GS accuracy was trait-, year-, and population structure-dependent. Conclusions The SNP markers identified from this study for plant height, maturity, seed weight and yield can be used to improve the four agronomic traits through marker-assisted selection (MAS) and GS in soybean breeding programs. After validation, the candidate genes can be transferred to new cultivars using SNP markers through MAS. The high GS accuracy has confirmed that the four agronomic traits can be selected in molecular breeding through GS.

scholarly journals Genetic Analysis of Some Genotypes of Indian Mustard (Brassica juncea L.) for Yield and Yield Attributing Traits

2021 ◽  
pp. 51-60
Author(s):  
A. M. Pradhan ◽  
M. Roy Choudhury ◽  
A. Sawarkar ◽  
S. Das
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Seed Weight ◽  
Block Design ◽  
Indian Mustard ◽  
Correlation Study ◽  
Biotic And Abiotic Stress ◽  
Randomized Block Design ◽  
Additive Gene ◽  
Positive Direct

The current investigation was carried out to examine the selection criteria for yield improvement in selected genotypes of Indian mustard. Twenty-four genotypes of Indian mustard were evaluated for seed yield and yield attributing characters with randomized block design with three replications. The analysis of variance showed significant differences among all the 24 genotypes against all the characters. The phenotypic and genotypic coefficient of variation were higher for most of the traits like seed yield per plant, number of secondary branches per plant and number of siliquae per plant. High heritability coupled with high genetic advance in percent of mean was registered for number of siliquae per plant, number of primary and secondary branches per plant, plant height, 1000 seed weight, siliqua length per plant and seed yield per plant suggesting predominant role of additive gene action for expression of these traits. The correlation study revealed that seed yield per plant was positively and significantly correlated with number of primary and secondary branches per plant, number of siliquae per plant, siliqua length per plant and 1000 seed weight at both phenotypic and genotypic level. The traits namely, number of secondary branches per plant recorded as highest positive direct effect followed by number of seeds per siliqua, number of siliquae per plant and plant height. Therefore, they may be carried out further evaluation in multilocational trials, biotic and abiotic stress like environmental conditions to check their stability and adaptability.

Heterosis, inbreeding depression and their relationship with genetic divergence in sesame ( Sesamum indicum L.)

2010 ◽  
Vol 58 (3) ◽  
pp. 313-321 ◽  
Author(s):  
P. Banerjee ◽  
P. Kole
Keyword(s):  
Plant Height ◽  
Inbreeding Depression ◽  
Seed Yield ◽  
Seed Weight ◽  
Block Design ◽  
Basic Material ◽  
Branch Number ◽  
Randomized Block Design ◽  
Component Traits ◽  
Pure Lines

Seven parents (CST2002, MT34, OS-Sel-2, TKG22, AAUDT9304-14-4, B67 and Rama), their 21 F 1 s and 21 F 2 s were grown in summer 2003 in a randomized block design with three replications. Heterosis and inbreeding depression were studied for seven important yield-contributing characters (plant height, branch number plant −1 , capsules plant −1 , seeds capsule −1 , 1000-seed weight, stick yield plant −1 and seed yield plant −1 ). Maximum heterosis for seed yield plant −1 over the mid- and better-parent was recorded in CST2002×TKG22 (43.30%) and MT34×B67 (27.22%), respectively. Mid-parent heterosis for seed yield plant −1 was due to cumulative heterosis for various important component traits, such as capsules plant −1 , seeds capsule −1 and 1000-seed weight. Inbreeding depression was highest for seed yield, followed by 1000-seed weight, capsules plant −1 , branch number and plant height, indicating the predominance of non-additive genetic effects. B67×Rama exhibited significant positive heterosis in F 1 , but non-significant inbreeding depression in F 2 for seed yield. This cross can be utilized as basic material for identifying better pure lines. The clustering pattern indicated that in general genetically diverse parents exhibited more heterosis, as evident in the majority of the crosses.

Conservation of leghemoglobin heterogeneity and structures in cultivated and wild soybean

1985 ◽  
Vol 63 (11) ◽  
pp. 1951-1956 ◽  
Author(s):  
W. H. Fuchsman ◽  
R. G. Palmer
Keyword(s):  
Glycine Max ◽  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Rhizobium Japonicum ◽  
Plant Introductions ◽  
Glycine Max L ◽  
Cultivated Soybean ◽  
Selection Of

The leghemoglobins from a genetically diverse selection of 69 cultivated soybean (Glycine max (L.) Merr.) cultivars and plant introductions and 18 wild soybean (Glycine soja Sieb. & Zucc.) plant introductions all consist of the same set of major leghemoglobins (a, c1, c2, c3), as determined by analytical isoelectric focusing. The conservation of both leghemoglobin heterogeneity and also all four major leghemoglobin structures provides strong circumstantial evidence that leghemoglobin heterogeneity is functional. Glycine max and G. soja produced the same leghemoglobins in the presence of Bradyrhizobium japonicum (Kirchner) Jordan and in the presence of fast-growing Rhizobium japonicum.

scholarly journals Inheritance of Some Quantitative Characters in Pigeonpeas (Cajanus cajan (L.) Mill sp.)

1969 ◽  
Vol 55 (1) ◽  
pp. 23-43
Author(s):  
Abad Morales Muñoz ◽  
Raúl Abrams
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Seed Weight ◽  
Block Design ◽  
Flowering Plant ◽  
Frequency Distributions ◽  
Flowering Date ◽  
Randomized Block Design ◽  
Genotypic And Phenotypic Correlations ◽  
High Heritability

Three varieties and an irradiation-derived line of pigeonpeas were used as parents in five crosses as follows: P.I. 5690 X Kaki, P.I. 5690 X Saragateado, P.I. 5690 X 526, Kald X 526, and Saragateado X 526. The F2, F3 and parents of each cross were planted in the field at the Isabela Substation during 1966-67 in a randomized block design with three replicates. Genotypic variability was studied and genotypic and phenotypic correlations were calculated for all the crosses in the F2 and F3 generations. Genetic coefficients of variation and heritability estimates were computed for four traits in five crosses and for six traits in one particular cross. Frequency distributions are shown for date of flowering, plant height, and seed weight. There was much greater variation for all crosses, in general, in seed weight, plant height, and flowering date than in number of seeds per pod. With the exception of number of pods per plant the correlations between seed yield and other traits were not great enough to provide reliable indications for yield. The phenotypic and genotypic correlations between seed yield and number of pods per plant were high, above 0.90. However, their low heritability, 45.3 and 52.1 percent in F2 and F3 generations, tends to indicate that not too much progress could be expected for seed yield improvement by selecting for large number of pods per plant. Good progress by selection could be made by breeders in traits such as flowering date, plant height, and seed weight which showed high heritability values.

scholarly journals Inter-Relationship between Seed Yield and Some Morphological and Biochemical Traits in Lentil (Lens culinaris L.)

2021 ◽  
Vol 9 (4) ◽  
pp. 146-151
Author(s):  
G. S. Mandal ◽  
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Indirect Effect ◽  
Seed Weight ◽  
Seed Protein ◽  
Genetic Advance ◽  
Seed Protein Content ◽  
Leaf Chlorophyll ◽  
100 Seed Weight ◽  
Pod Number

Correlation Study with Path analysis was performed on 29 Lentil Genotypes to determine direct and indirect effect of some morphological as well as Biochemical parameters on Yield. Following traits were studied: Root Nodule number, Nodule weight, Primary Root Length, Secondary root numbers, Root Fresh and Dry weight, Plant Height, Pods per plant, Days to 50% Flowering, 100 seed weight, Leaf chlorophyll content, Seed Carbohydrate with Starch content, Seed Protein content and Yield. Considering both genetic Advance and GCV, it was observed that selection for the following characters viz. Plant Height, Pods per plant, Days to 50% flowering, 100 seed weight, Seed Protein content & Yield will be rewarding. Seed Yield per plant showed significantly Positive correlation with the following traits: 100 Seed weight, Pod number per plant, Plant height and seed starch. Negative association with yield was found for Days to 50% flowering. Path coefficient study revealed that there was a strong positive direct effect of 100 seed weight and Pod no. per plant along with Leaf Chlorophyll content on Yield. Hence, direct selection only for Pods number and 100 seed weight will be rewarding as these characters also gave higher heritability % along with high Genetic advance. Plant height gave strong negative direct effect on yield, but it gave a positive correlation due to high indirect effect in association with pod number. It may be due to short winter span of West Bengal which results into forced maturity of the crops. So short plants with higher pod numbers will be desirable for higher yield in short winter span condition.

scholarly journals Performance of Chickpea (Cicer arietinum L.) Genotypes in Sunsari district of Province no. 1 Nepal

2020 ◽  
Vol 1 (2) ◽  
pp. 62-67
Author(s):  
◽  
Sujan Karki ◽  
Binod Gupta ◽  
Rajendra Darai ◽  
Sabita Sharma ◽  
...  
Keyword(s):  
Plant Height ◽  
Yield Components ◽  
Seed Weight ◽  
Insect Pests ◽  
Disease Incidence ◽  
Block Design ◽  
Growth Yield ◽  
Seed Yields ◽  
Pod Number ◽  
Seed Diameter

The present study was carried out to know the performance of growth, yield contributing characters, and reaction against insect pests and disease on chickpea genotypes at Jute Research Program, Itahari, Sunsari, Nepal. A total of twelve chickpea genotypes were sown in Randomized Complete Block Design (RCBD) with three replications and each replicate had 10 lines with an inter and intra row spacing of 40 cm and 10 cm respectively. It is of great interest to consider the per se performance of different genotypes on various characters of economic importance, particularly earliness, plant height, nodule number, pod number, seed diameter, 100 seed weight, seed yields, pest and disease incidence. The genotypes ICCV-87312 showed earlier in flowering and maturity while the genotypes KWR-108 and Tara showed the highest and lowest plant height respectively. Likewise, the yield and yield components of overall pooled mean performance of chickpea genotypes ICCV-840508-38 born the maximum pod number, seed diameter, hundred seed weight, and seed yields. With respect to pest incidence, genotype KWR-108 was found to be less susceptible while genotype Tara was found to be more susceptible against pest damage (pod damage). Similarly, the genotypes ICCV-87312 found to be less susceptible while genotypes ICCV-98937 were found to be more susceptible against fusarium wilt disease among the tested genotypes. On the basis of the mean performance of yield components and biotic stress components observed in the present study, the five genotypes viz., ICCV-840508-38, ICCV-98933, KPG-59, ICCV-87312, and KWR-108 were found to be superior genotypes. Therefore, farmers and chickpea producers around study areas and similar agro-ecologies can use those genotypes for chickpea production as well as these materials can be used for the further breeding programs too.

scholarly journals Evaluating Performance of Sesame (Sesamum indicumL.) Genotypes in Different Growing Seasons in Northern Ethiopia

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Fiseha Baraki ◽  
Muez Berhe
Keyword(s):  
Grain Yield ◽  
Seed Weight ◽  
Agronomic Traits ◽  
Block Design ◽  
Sub Saharan Africa ◽  
Northern Ethiopia ◽  
Growing Seasons ◽  
Randomized Complete Block Design ◽  
Sub Saharan ◽  
Thousand Seed Weight

Ethiopia is one of the famous and major producers of sesame in sub-Saharan Africa, and Ethiopian sesame is among the highest quality in the world. The experiment was conducted in Northern Ethiopia for three growing seasons (2013–2015) under a rain fed condition with the objective of identifying high-yielding genotypes and their agronomic traits. The experiment consisted of twelve genotypes laid down in randomized complete block design with three replications. The genotype, year, and genotype × year interaction components showed statistically highly significant variation (p<0.001) for most of the agronomic traits which clearly confirms the presence of genotype × year interaction in this study. The highest combined mean grain yield (906.3 kg/ha) was obtained from Hirhir followed by Serkamo white (756.5 kg/ha), and from the three growing seasons, the highest grain yield (1161.5 kg/ha) was recorded from Hirhir grown in the second growing season (2014). The growing seasons were different from one another in allowing the genotypes to have a different performance, and all of the agronomic traits, except thousand seed weight, were statistically different across the three growing seasons. In the ordination of the genotypes and agronomic traits, PCA1, which accounted for 38.3% of the variation, was positively associated with grain yield, branches per plant, length of the pod-bearing zone, plant height, number of pods per plant, number of seeds per pod, and thousand seed weight. On the contrary, PCA2, which accounted for 19.7% of the variation, was positively associated with days to 50% flowering and days to 50% maturity.

KERAGAAN GENERASI KETIGA (M3) KEDELAI HASIL IRADIASI SINAR GAMMA PADA KONDISI OPTIMUM DAN KONDISI CEKAMAN KEKERINGAN

2015 ◽  
Vol 2 (1) ◽  
pp. 44-51
Author(s):  
Diana Sofia Hanafiah
Keyword(s):  
Optimum Condition ◽  
Plant Height ◽  
Seed Weight ◽  
Agronomic Traits ◽  
Stress Conditions ◽  
Control Population ◽  
Optimum Conditions ◽  
Drought Conditions ◽  
Putative Mutant

The objective of this research was to obtain a genotype of selected putative mutant withgood agronomic traits and high yielding on the optimum condition and its adaptability todrought stress in M3 generation. The study result showed that there were differences in thegrowth and production of each population as a result of irradiation in optimum conditions anddrought stress conditions. The population as a result of irradiation at 150 Gy and 200 Gy had a highermean value for the plant height trait, the number of productive branches, the number of productivenodes and the number of productive pods compared to irradiation population of 50 Gy, 100 Gy andthe control population of Argomulyo at optimum conditions. The decrease in the growth anddevelopment happened to all the characters observed in the irradiation population in droughtconditions compared to when grown in optimum conditions. The decrease can be seen in thecharacters of plant height, the number of pods and seed weight per plant. The growth and developmentof the population irradiation of 150 Gy and 200 Gy were more influenced by drought conditionscompared to other irradiation populations. The selection in the optimum conditions produced 291high yielding genotypes and the selection in drought conditions generated 202 genotypes andadapted to drought environments.

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