Viability, productivity, and anatomical response of groundnuts (Arachis hypogaea L.) to biofertilizer-sludge biogas applications

Groundnuts (Arachis hypogaea L.) is one of the essential food commodities in Indonesia. The use of biofertilizer has been applied to various types of crops. Meanwhile, the effect of using biofertilizer-sludge biogas on groundnuts is yet unknown. This study aims to analyze the seed viability and vigour, yield productivity, the anatomical response of groundnuts, and optimum concentration that could increase the values of each parameter. Treatments given include applying biofertilizer-sludge with 15 levels of treatment concentration compared to groundnuts without biofertilizer-sludge application as a control. The land was divided into 16 beds for each treatment consisting of control, biofertilizer from 10, 15, 30 L/ha, sludge from 12, 24, to 36 ml, and variations dosage of biofertilizer and sludge combined. The parameters observed for viability and vigour include the percentage of seed germination (GP), seed vigor index (SVI) for yield, the value of harvest index (HI), dry weight of the harvest, and root-shoot ratio (R/S). Anatomical responses were observed with stem diameter, stem’s metaxylem diameter, root diameter, root’s metaxylem diameter, and seed diameter. The biofertilizer-sludge results significantly affected HI, R/S values, stem diameter, root’s metaxylem diameter, and seed diameter. This research concluded that the application of biofertilizer-sludge did not significantly affect the seed viability and vigour and the dry weight of the harvest. The application of biofertilizer-sludge in various doses of concentration resulted in a decrease in the stem metaxylem diameter and root diameter compared to the control. A total of 10 L/ha biofertilizer + 24 ml sludge was an optimum concentration to increasing HI and R/S values. For the increasing stem, root metaxylem, and seed diameter, biofertilizer 30 L/ha + sludge 12 ml, sludge 24 ml, and biofertilizer 15 L/ha + sludge 12 ml were the optimum concentrations, respectively.

Performance of Chickpea (Cicer arietinum L.) Genotypes in Sunsari district of Province no. 1 Nepal

The present study was carried out to know the performance of growth, yield contributing characters, and reaction against insect pests and disease on chickpea genotypes at Jute Research Program, Itahari, Sunsari, Nepal. A total of twelve chickpea genotypes were sown in Randomized Complete Block Design (RCBD) with three replications and each replicate had 10 lines with an inter and intra row spacing of 40 cm and 10 cm respectively. It is of great interest to consider the per se performance of different genotypes on various characters of economic importance, particularly earliness, plant height, nodule number, pod number, seed diameter, 100 seed weight, seed yields, pest and disease incidence. The genotypes ICCV-87312 showed earlier in flowering and maturity while the genotypes KWR-108 and Tara showed the highest and lowest plant height respectively. Likewise, the yield and yield components of overall pooled mean performance of chickpea genotypes ICCV-840508-38 born the maximum pod number, seed diameter, hundred seed weight, and seed yields. With respect to pest incidence, genotype KWR-108 was found to be less susceptible while genotype Tara was found to be more susceptible against pest damage (pod damage). Similarly, the genotypes ICCV-87312 found to be less susceptible while genotypes ICCV-98937 were found to be more susceptible against fusarium wilt disease among the tested genotypes. On the basis of the mean performance of yield components and biotic stress components observed in the present study, the five genotypes viz., ICCV-840508-38, ICCV-98933, KPG-59, ICCV-87312, and KWR-108 were found to be superior genotypes. Therefore, farmers and chickpea producers around study areas and similar agro-ecologies can use those genotypes for chickpea production as well as these materials can be used for the further breeding programs too.

GENETIC DIVERSITY, HERITABILITY AND CORRELATION BETWEEN LOCAL CULTIVARS OF PADDY GOGO

Paddy is one of the important cereal crops as food a main food for a third of the world's population, including Asia. Production of paddy rice plants can be improved through plant genetic improvement. The ebjective of this study was examine the genetic diversity, heritability, and correlation between the characteristics of several local upland rice cultivars. This study was carried out in Tamarenja Village, Sindue District, Donggala Regency, Central Sulawesi Province. It was began in May to October 2018. A Randomized Block Design with eight cultivars as a genotypic treatment and reapeted three times was used in this study. There were 24 trial plots in total. Variables observed included plant height, number of leaves, length of leaves, number of tillers, number of productive tillers, age of panicle exit, panicle length, harvest age, number of seeds per panicle, seed length, seed diameter, weight of 100 seeds, and total yield. The results showed that the genetic influences were more dominant than the environment on all observed variables except the weight of 100 seeds and seed diameter. The number of seeds per panicle, the number of tillers and the number of productive tillers were traits that have a high value on heritability and genetic progress. Panicle length, seed diameter and weight of 100 seeds were characteristics that have a high genetic correlation coefficient on upland rice yields. The weight of 100 seeds has a direct influence and high genetic correlation to the results so that it can be used as a direct selection criterion to increase upland rice yields.

THE APPEARANCE OF PHENOTYPIC CHARACTER ON FIVE GENOTYPES OF CAYENNE PEPPER

This study was aimed at determining the appearance of phenotypic characters in five cayenne genotypes in Sukamantri, Ciamis Regency. The research method used was the experimental method with Randomized Block Design (RBD) with five treatments repeated five times. Data were analyzed by variance and followed by Least Significant Difference (LSD) Test. The testing material consisted of five genotypes of cayenne pepper, namely one of the new superior varieties (Ratuni Unpad), and four varieties (CR8873, Dewata, Taruna, and Rabani). The tests were carried out in Sukamantri, Ciamis Regency in 983 m asl in August 2017 to February 2018. The observations were made on the character of the results and components of results which included the seed length, the seed diameter, the seed weight per unit, the seed weight per plant, the seed weight per plot, and the seed weight per hectare. The test results showed that there were differences in phenotypic characters between the five genotypes tested. The Dewata, Ratuni Unpad, and CR8873 genotypes show the highest potential yield (8.34 tons/ha, 7.71 tons/ha, and 7.24), with the character of fruit weights per fruit of 1.382 grams, 0.911 grams respectively. and 1,311 grams.PENAMPILAN KARAKTER FENOTIPIK HASIL PADA LIMA GENOTIP CABAI RAWITPenelitian ini bertujuan untuk mengetahui penampilan karakter fenotipik pada lima genotip cabai rawit di Sukamantri, Kabupaten Ciamis. Metode penelitian yang digunakan adalah metode eksperimen dengan Rancangan Acak Kelompok (RAK) dengan lima perlakuan diulang lima kali. Data dianalisis dengan sidik ragam dan dilanjutkan dengan Uji Least Significant Difference (LSD). Bahan pengujian terdiri atas lima genotip cabai rawit, yaitu salah satu varietas unggul baru (Ratuni Unpad), dan empat varietas (CR8873, Dewata, Taruna, dan Rabani). Pengujian dilakukan di Sukamantri, Kabupaten Ciamis dengan ketinggian tempat 983 m di atas permukaan laut (dpl.) pada Agustus 2017 sampai Februari 2018. Pengamatan dilakukan terhadap karakter hasil dan komponen hasil yang meliputi panjang buah, diameter buah, bobot buah per buah, bobot buah per tanaman, bobot buah per plot, dan bobot buah per hektar.Hasil pengujian menunjukkan bahwa terdapat perbedaan karakter fenotipik antarlima genotip yang diuji. Genotip Dewata, Ratuni Unpad, dan CR8873 menampilkan potensi hasil yang tertinggi (8,34 ton/ha; 7,71 ton/ha; dan 7,24); dengan karakter bobot buah per buah masing-masing sebesar 1,382 gram; 0,911 gram; dan 1,311 gram.

Effects of day length on pollen tube elongation, embryo formation and seed development after flowering in quinoa (Chenopodium quinoa Willd.)

The objective of this study was to evaluate the effect of day length after flowering on pollen tube elongation, embryo formation and seed development. The quinoa varieties used in this study were Amarilla de Marangani (valley type) and NL-6 (sea-level type). After sowing, the quinoa plants were cultivated in growth cabinets. From sowing to flowering, plants were exposed to a 15 h day length regime. After flowering, the plants were grown under either a 15 h or 11 h day length regime. The elongation of the pollen tube and the formation of the early embryo were not inhibited in either Amarilla de Marangani or NL-6 under the 11 or 15 h day length regimes. Although growth of the embryo in NL-6 was not inhibited by the 15 h day length regime after flowering, the same was not observed in the case for Amarilla de Marangani. In Amarilla de Marangani, seed diameter at 8 and 14 days after flowering under the 11 h day length regime was larger than that of seeds grown under the 15 h day length regime. Thus, the decrease in the number of seeds in Amarilla de Marangani grown under the 15 h day length regime may be caused by the suspension of embryo growth after fertilization.

Germinación, diámetro de semilla y tratamientos pregerminativos en especies con diferentes finalidades de uso

The evaluation of vegetal species germination consents to know its characteristics and permits to understand the factors that influence this process. The aim of this research was to know the germination’s characteristics of some species, such as Eugenia stipitata McVaugh, Inga edulis Mart, Inga spectabilis (Vahl) Wild, Piptocoma discolor (Kunth) Pruski, Stachytarpheta cayennensis (Rich.) Vahl, and Verbena officinalis L., and also their reaction to pregerminative treatments depending on the seed’s diameter. This study was carried out in Pastaza, Province of Pastaza, Ecuador, between February and June, 2014. Different diameters of seeds and pregerminative treatments were used in species, which did not present germination percentages higher than 40%. In the first practice I. edulis and I. spectabilis exceeded this value without treatment. Other species had lower values. Seeds were classified considering two diameters and two doses of gibberellin acid, this was applied to, and evaluated in the E. stipitata. In addition, scarification with sulfuric acid was done. After 45 days of its application, 100 ppm of gibberellic acid with larger seed diameter reported higher percentages of germination in S. cayenennsis, and in E. stipitata, which also interacted with the scarification. V. officinalis and P. discolor, did not present any response to the applications made. I. edulis and I. spectabilis presented high germination percentages without pregerminative treatments, E. stipitata and S. cayenennsis showed response to seed diameter and the applied treatments, while P discolor and V. officinalis did not show any response.

MORPHOMETRIC CHARACTERISTICS, PROPORTITION, TOTAL FENOL CONTENT AND PROFIL PHENOLICS OF AVOCADO (Persea americana, Mill) PULP, SEED AND PEEL VARIETY OF IJO PANJANG AND IJO BUNDAR

The aims of the study were to compare morphometrics chararcteristic, porportion, total phenolics and profil phenoilic of pulp, seed and peel of avocado (Persea americana, Mill) variety of ijo panjang and ijo bundar. Morphometrics characteristics were measured by using ruler and calipers. Fruit proportions were determined by using digital balance, Total phenolics was analized by Folin-Ciocalteu methods and profil phenolics were analized by using HPLC. The result of the studies found that variety of ijo panjang had longer size, smaller seed diameter and thicker peel than ijo bundar. Seed proportion variety ijo panjang was lower than ijo bundar, but pulp and peel were higher. Total phenolics content of ijo panjang lower in pulp and seed, but higher in peel. Profil phenolics compound in avocado consist of cathechin, hydroxybenzoic acid, flavonol and procyanidin and hydroxycinnamic acid. Phenolics compounds in pulp were higher in ijo panjang variety, except hydroxycinnamic acid. Phenolics compounds in seed was higher in variety of ijo bundar. Phenolics Compounds in peel were hinger in ijo panjang, except hydroxybenzoic acid.

Inheritance and Linkage Map Positions of Genes Conferring Agromorphological Traits inLens culinarisMedik.

Agromorphological traits have immense importance in breeding lentils for higher yield and stability. We studied the genetics and identified map positions of some important agro-morphological traits including days to 50% flowering, plant height, seed diameter, 100 seed weight, cotyledon color, and growth habit inLens culinaris. Earlier developed RILs for stemphylium blight resistance (ILL-5888 × ILL-6002), contrasted for those agro-morphological traits, were used in our study. Three QTLs for days to 50% flowering were detected with additive and epistatic effects. One QTL for days to 50% flowering, QLG483(QTL at linkage group 4 at 83 cM position), accounted for an estimated 20.2% of the variation, while QLG124 × QLG1352and QLG484 × QLG138accounted for 15.6% and 24.2% of the variation, respectively. Epistatic effects accounted for most of the variation in plant height, but the main effect of one QTL, QLG84, accounted for 15.3%. For seed diameter, three QTLs were detected, and one QTL, QLG482, accounted for 32.6% of the variation. For 100 seed weight, five QTLs were identified with significant additive effects and four with significant interaction effects. The main effect of one QTL, QLG482, also accounted for 17.5% of the variation in seed diameter. QLG482-83which appears to affect days to 50% flowering, seed diameter, and 100 seed weight is flanked by RAPD markers, UBC 34 and UBC1. Growth habit and cotyledon color are controlled by single genes with prostrate dominant to erect and red cotyledon dominant to yellow. The QTL information presented here will assist in the selection of breeding lines for early maturity, upright growth habit, and improved seed quality.

In vivo grafting of wild Lens species to Vicia faba rootstocks

Faba bean (Vicia faba L.) was used as the rootstock for lentil scions to test the feasibility of using in vivo inter-generic grafting techniques as a substitute for root induction and as a tool in lentil genetic improvement. An accession of each of the six wild Lens species was used as the scion in grafts to faba bean breeding line FB50-9 rootstock. Successful grafts were obtained for all species with survival of grafts to seed maturity between 70.7 and 87.7% except for Lens orientalis PI 72735 with 55.3% survival. Days to flower remained the same after grafting, except for scions of L. nigricans PI 72560 and L. orientalis PI 72735 which had a lag phase of 9 and 7 d, respectively. For all six wild species, seed diameter and seed weight were not significantly different between non-grafted controls and scions grafted onto faba bean rootstocks. This simple approach opens the possibility of using in vivo grafting techniques to rescue inter-specific hybrids of lentil. The technique has potential as a useful tool in lentil breeding, as a means of improving seed multiplication rate of rare genetic resources of wild lentil and as a way to reduce the costs of germplasm multiplication of wild lentil species.

Models to Describe Cycas micronesica Leaf and Strobili Development

Cycas micronesica leaf and strobili expansion patterns were measured in three locations and seasons on Guam and then were fitted with non-linear models to evaluate the use of the parameters for informing management decisions. All growth curves except for microstrobili height conformed to a negative exponential function. Microstrobili height development could not be fitted with any traditional linear or non-linear function, so spline models were used to smooth the effect of elapsed days. Leaf and leaflet expansion patterns were influenced by habitat and season, indicating development of the vegetative organ is plastic. In contrast, the models that described development of megasporophyll length, microstrobili height, and microstrobili diameter were not influenced by habitat or season. Moreover, seed diameter developmental patterns were only minimally influenced by location. These results indicate developmental patterns of the reproductive structures were primarily constitutive. We have demonstrated two empirical approaches to fitting models of Cycas micronesica organ growth and development and that both methods are useful for determining the influence of spatial or temporal factors in the timing of organ development. This approach may be used to inform horticultural or conservation questions of other rare cycad species.