scholarly journals Migration Inhibition Induced by Gypenosides and Its Combination Effect with 5-fluorouracil on Human Colon Cancer SW-620 Cells

2021 ◽  
Vol 2 (4) ◽  
Author(s):  
Xiaoxia Cheng ◽  
Shao'an Xue ◽  
Zijian Wang ◽  
Fengqin He ◽  
Bo Wang
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Inhibitory Effect ◽  
Migration Inhibition ◽  
Human Colon Cancer ◽  
Killing Effect ◽  
Adhesion Ability ◽  
Migration Potential ◽  
Combination Studies ◽  
Cell Killing Effect

In this study we investigate the migration inhibition of Gypenosides (Gyp) and its combined effects with 5-fluorouracil (5-FU) on human colon cancer SW-620 cells, hoping to explore more potential clinical use of Gyp. Our data implied Gyp could significantly inhibit the migration potential of SW-620 cells including down-regulating matrix Metalloproteinases expression and decreasing cells adhesion ability. What’s more, evidence showed cells treated with Gyp exerted serious microfilament network collapse as well as a significant decline in the number of microvilli. A significant migration inhibitory effect was seen in Gyp groups along with the decline of cell adhesion. Further, the combination studies suggested Gyp could synergistically enhance the antitumor effect of 5-FU in SW-620 cells through the apoptosis way. The present study indicated Gyp could prevent cell migration and further enhance the cell killing effect of 5-FU on human colon cancer SW-620 cells.

scholarly journals 3-Bromopyruvate potentiates TRAIL-induced apoptosis in human colon cancer cells through a reactive oxygen species- and caspase-dependent mitochondrial pathway

2019 ◽  
Vol 97 (12) ◽  
pp. 1176-1184 ◽  
Author(s):  
Hassan Abbaszadeh ◽  
Armita Valizadeh ◽  
Masoud Mahdavinia ◽  
Ali Teimoori ◽  
Mohammad Hassan Pipelzadeh ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Human Colon ◽  
Inhibitory Effect ◽  
Oxygen Species ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Normal Cells

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a promising anticancer cytokine with minimal toxicity towards normal cells. Nevertheless, most primary cancers are often intrinsically TRAIL-resistant or can acquire resistance after TRAIL therapy. This study aimed to investigate the inhibitory effect of co-treatment of 3-bromopyruvate (3-BP) as a potent anticancer agent with TRAIL on colon cancer cells (HT-29). The results of present study indicated that combined treatment with 3-BP and TRAIL inhibited the proliferation of HT-29 cells to a greater extent (88.4%) compared with 3-BP (54%) or TRAIL (11%) treatment alone. In contrast, the combination of 3-BP and TRAIL had no significant inhibitory effect on the proliferation of normal cells (HEK-293) (8.4%). At a cellular mechanistic level, the present study showed that 3-BP sensitized human colon cancer cells to TRAIL-induced apoptosis via reactive oxygen species generation, upregulation of Bax, downregulation of Bcl-2 and survivin, release of cytochrome c into the cytosol, and activation of caspase-3. In normal cells, 3-BP, TRAIL, or combination of both had no significant effect on the reactive oxygen species levels, release of cytochrome c, and caspase-3 activity. Therefore, the combination of 3-BP and TRAIL can be a promising therapeutic strategy for treatment of colon cancer.

scholarly journals Killing effect of CD/5-FC system on human colon cancer cell lines SW 480 and LoVo

2003 ◽  
Vol 11 (5) ◽  
pp. 535-539
Author(s):  
Cheng-Jin Li ◽  
Qing-Jiu Ma ◽  
Da-Nian Lai ◽  
Jiang-Guo Lu ◽  
Xiao-Jun Wang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Killing Effect ◽  
Colon Cancer Cell Lines

Inhibition of Leukocyte Migration by a Human Colon Cancer Extract

1981 ◽  
Vol 67 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Maria Caterina Sirianni ◽  
Giuseppe Luzi ◽  
Claudio Iavarone ◽  
Marisa Papaluca ◽  
Massimo Fiorilli ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Control Group ◽  
Migration Inhibition ◽  
Human Colon Cancer ◽  
Tumor Associated Antigen ◽  
Leucocyte Migration ◽  
Tumor Extract ◽  
Leucocyte Migration Inhibition Test ◽  
Leucocyte Migration Inhibition

The direct leucocyte migration inhibition test in capillary tubes was used to test 10 patients with colonic adenocarcinoma against KCl soluble extracts of an allogenic colon cancer and an allogenic normal colon fragment. Inhibition was consistently found with the cancer extract but not with the normal tissue extract. None of the control group of patients affected by other tumors, intestinal and liver disorders showed a migration inhibition in response to the colonic tumor extract. Our findings strongly suggest the presence of a tumor-associated antigen in the cancer extract.

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