scholarly journals Protein Extractability and Thermally Induced Gelation Properties of African Locust Bean Proteins (Parkia biglobosa Jacq. R.Br)

2021 ◽  
Vol 10 (1) ◽  
pp. 19-27
Author(s):  
Mamadou Lamarana Souare ◽  
Lonseny Traore ◽  
Florence Husson ◽  
Samuel Lubbers
Keyword(s):  
Ionic Strength ◽  
Protein Concentration ◽  
Extraction Process ◽  
Extraction Yield ◽  
Thermally Induced ◽  
Factors Affecting ◽  
Seed Flour ◽  
Locust Bean ◽  
African Locust Bean ◽  
Protein Extractability

African locust bean (AfLB) protein isolates could be an interesting alternative to the use of soy as an ingredient for the development of new protein-rich products. From AfLB seed flour, the protein extractability yields by aqueous extraction were determined as a function of pH and ionic strength. Then thermally induced gelation of various protein suspensions relating to protein concentration was studied. The most critical factors affecting extractability were the pH and the presence of fat. As a function of the extraction process, the extraction yield of protein from AfLB flour ranged from 30 to 65%. Two major fractions of proteins detected in AfLB seeds were albumins and globulins, comprising four families: legume-like protein, vicilin-like proteins, convicilin, and albumins. The globulin isolate had the lowest solubility at pH 3.5-4 and the highest at pH 8-10. The solubility of albumin isolate was lightly affected by pH and ionic strength. At pH 7, the minimum protein concentration for thermal gel formation ranged from 55 to 120 g/L as function purified state of proteins. The less purified extract with a simpler process made it possible to obtain a gel needing a lower protein concentration. This last way seems promising to the development of new foods based on African locust bean flour.

scholarly journals Optimization of Insoluble and Soluble Fibres Extraction fromAgave americanaL. Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Amine Bouaziz ◽  
Manel Masmoudi ◽  
Amel Kamoun ◽  
Souhail Besbes
Keyword(s):  
Ionic Strength ◽  
Design Methodology ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Optimum Conditions ◽  
Box Behnken Design ◽  
Factors Affecting ◽  
Soluble Fibre ◽  
Experimental Design Methodology ◽  
Significant Factors

Experimental design methodology was used to determine significant factors affecting the extraction yield of soluble and insoluble fibres fromAgave americanaL. and in second time to find optimum conditions leading to the highest yield. Results clearly indicated that the temperature, the powder to water (P/W) ratio, and the agitation speed were the most important factors influencing fibres extraction yield which increased with temperature, P/W ratio, and agitation speed. Ionic strength affected significantly soluble fibre extraction yield and was the most important factor among nonsignificant ones influencing insoluble fibres extraction yield. Then, a Box-Behnken design was carried out to maximise fibres extraction. Selected optimal conditions were temperature: 90°C; P/W ratio: 0.1625; agitation speed: 400 rpm; and ionic strength: 1.5 g/L. These conditions yielded 93.02% and 80.46% of insoluble and soluble fibres, respectively. Concentrates showed high fibres purity and good functional properties.

scholarly journals Optimization of Extraction Process and the Antioxidant Activity of Phenolics from Sanghuangporus baumii

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3850
Author(s):  
Na Zheng ◽  
Yongfei Ming ◽  
Jianzhi Chu ◽  
Shude Yang ◽  
Guochao Wu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Extraction Yield ◽  
Fruiting Bodies ◽  
Factors Affecting ◽  
Medicinal Fungus

Sanghuangporus baumii, is a widely used medicinal fungus. The polyphenols extracted from this fungus exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, polyphenols from the fruiting bodies of S. baumii were obtained using the deep eutectic solvent (DES) extraction method. The factors affecting the extraction yield were investigated at different conditions. Based on the results from single-factor experiments, response surface methodology was used to optimize the extraction conditions. The scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was determined. The results showed that the DES system composed of choline chloride and malic acid had the best extraction yield (6.37 mg/g). The optimal extraction parameters for response surface methodology were as follows: 42 min, 58 ℃, 1:34 solid–liquid (mg/mL), and water content of 39%. Under these conditions, the yield of polyphenols was the highest (12.58 mg/g). At 0.30 mg/mL, the scavenging ability of the polyphenols on •OH, DPPH, and ABTS+ was 95.71%, 91.08%, and 85.52%, respectively. Thus, the method using DES was more effective than the conventional method of extracting phenolic compounds from the fruiting bodies of S. baumii. Moreover, the extracted polyphenols exhibited potent antioxidant activity.

Extraction Process of Amaranthine Pigment Keeping the Water Wax Fuit Biological Active

2013 ◽  
Vol 295-298 ◽  
pp. 150-154
Author(s):  
Feng Jie Wang ◽  
Xin Qiao Dong ◽  
Xiao Liu
Keyword(s):  
Citric Acid ◽  
Extraction Method ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Natural Pigment ◽  
Pigment Extraction ◽  
Acid Aqueous Solution ◽  
The Stability

Development natural pigment retained a variety of nutrition composition is extremely research value.Water Wax Trees Fruit was as raw materials,citric acid aqueous solution was as extracting agent.Amaranthine pigment extraction method were studied on fully grasping the stability of the natural amaranthine pigment and no damage to the water wax fruit biological activity.The effect of extraction yield were studied on Water Wax Trees Fruit natural amaranthine pigment.The results show that the better extraction conditions are extraction temperature at 70 °C, extraction time for 3h,citric acid concentration1.0 % , the material/liquid ration(g/ml) 1:5.The extraction yield is 2.95%.Ultraviolet absorption maximum wavelength is 280 nm.

Separation of Carotenoids from Physalis Alkekengi var. Francheti through Supercritical CO2

2011 ◽  
Vol 236-238 ◽  
pp. 2414-2417 ◽  
Author(s):  
Zhen Huang ◽  
Mei Jing Yang ◽  
Qing Ma ◽  
Shao Fang Liu
Keyword(s):  
Particle Size ◽  
Diffusion Layer ◽  
Processing Time ◽  
Extraction Process ◽  
Theory Model ◽  
Extraction Yield ◽  
Total Carotenoid ◽  
Relative Deviation ◽  
Time Profiles

Separation of carotenoids has been carried out on the calyx of Physalis alkekengi var. francheti by means of supercritical CO2. The influences of various parameters such as extraction pressure, temperature, processing time and particle size on the total carotenoid yield extracted are investigated in detail. A diffusion layer theory model is used to correlate the extraction yield vs. time profiles and it performs rather satisfactorily to describe the extraction process with an average absolute relative deviation varying from 3.2 to 11.0%.

scholarly journals An Electrophoretic Study on "Component 2" Extracted From Wool With Alkaline Thioglycollate

1955 ◽  
Vol 8 (3) ◽  
pp. 378 ◽  
Author(s):  
JM Gillespie ◽  
FG Lennox
Keyword(s):  
Ionic Strength ◽  
Protein Concentration ◽  
Electrophoretic Pattern ◽  
Electrophoretic Study ◽  
Moving Peaks ◽  
Wool Keratin

Solutions of the thioglycollate-reduced wool keratin preparation, "component 2" of Gillespie and Lennox (1953, 1955), show abnormal electrophoretic behaviour. New, faster moving peaks appear in the descending electrophoretic pattern at protein concentrations exceeding 0�5 per cent. which are attributed to an aggregation-disaggregation reaction. They are eliminated by increasing the ionic strength to 0�5, or by lowering the protein concentration to 0�4 per cent.

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