scholarly journals The Role of the Plasma Membrane and Tonoplast in Salt-Tolerance: Characterization of the Atpases and Ion Transport Properties

1987 ◽  
Author(s):  
Gozal Ben-Hayyim ◽  
Frances DuPont ◽  
Robert Lundin ◽  
John Windle ◽  
William Hurkman
Keyword(s):  
Plasma Membrane ◽  
Salt Tolerance ◽  
Ion Transport ◽  
Transport Properties

scholarly journals Characterization of interactions between the soybean plasma membrane- intrinsic proteins GmPIP1s and GmPIP2s responding to salt stress

2020 ◽  
Author(s):  
Weicong Qi ◽  
Jia Liu ◽  
Dayong Zhang ◽  
Haiying Lu ◽  
Hongbo Shao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Vital Role ◽  
Plant Responses ◽  
Plasma Membrane Intrinsic Proteins ◽  
Physiological And Biochemical ◽  
Salt And Osmotic Stress

Abstract Background: Salt tolerance is a key trait in soybean breeding and plant responses to salt stress include physiological and biochemical changes that affect the movement of water across the plasma membrane. In this study, we report the interactions of a set of aquaporins, soybean (Glycine max) plasma membrane-intrinsic proteins (GmPIPs), in response to salt stress. Results: GmPIP1;5 and GmPIP1;6 formed hetero-tetramers with GmPIP2;4, GmPIP2;6, GmPIP2;8, GmPIP2;9, GmPIP2;11, and GmPIP2;13. We detected interactions between GmPIP1;6 and GmPIP1;7, but not between GmPIP1;6 and GmPIP1;5. Furthermore, GmPIP2;9 formed homo-tetramers, and this interaction was strengthened under salt and osmotic stress. Expression analysis indicated complex and unique responses to salt stress depending on the duration of the stress. For example, GmPIP2;8, encoding one of the heteromer-forming PIP proteins, was highly up-regulated under early salt stress.Conclusions: Our study highlights the vital role of hetero- and homo-tetramers, in salt tolerance; and improves understanding of the mechanisms by which soybean aquaporin isoforms respond to abiotic stress.

scholarly journals RsSOS1 Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na+ Homeostasis in Radish (Raphanus sativus L.)

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 458
Author(s):  
Wanting Zhang ◽  
Jingxue Li ◽  
Junhui Dong ◽  
Yan Wang ◽  
Liang Xu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Functional Characterization ◽  
Vital Role ◽  
Salt Stress Response ◽  
Reading Frame ◽  
Yield And Quality

Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na+/H+ antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the RsSOS1 gene in radish remains to be elucidated. In this study, the RsSOS1 gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with Arabidopsis plasma membrane Na+/H+ antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, RsSOS1 was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of RsSOS1 in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of RsSOS1△999 could rescue Na+ efflux function of AXT3 yeast. In summary, the plasma membrane Na+/H+ antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na+ homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish.

scholarly journals Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing

ExRNA ◽  
2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Fangfang Jin ◽  
Yuling Sun
Keyword(s):  
Salt Tolerance ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Procambarus Clarkii ◽  
Economic Losses ◽  
Srna Library ◽  
Genome Wide ◽  
A Genome

Abstract Procambarus clarkii is one of the important economic species in China and has been served as tasty food in recent years after being introduced to Nanjing. Significant problems of environment factors, such as salinity, pH and temperature, especially salinity, has the potential to result in significant economic losses in many crayfish-producing farms in China. miRNAs are a kind of ~ 22 nucleotide small non coding RNAs which were encoded by plants, animals and some viruses with functions in RNA silencing or post-transcription regulation. We constructed four sRNA library of P. clarkia from different tissues and treatments by using high-throughput sequencing technology. A total of 101 conserved miRNAs and two novel pre-miRNAs were identified and RT-qPCR were further performed to confirm existence of part of identified miRNAs. A genome wide expression profile of salt-tolerance miRNAs was proved and three miRNAs were further validated by RT-qPCR with dynamic response to different salinity stages. The study of miRNAs in P. clarkia can help us better understanding the role of miRNAs in salt-tolerance in P. clarkia.

scholarly journals Room temperature structure and transport properties of the incommensurate modulated LaNb0.88W0.12O4.06

2019 ◽  
Vol 48 (5) ◽  
pp. 1633-1646 ◽  
Author(s):  
Cheng Li ◽  
Stevin S. Pramana ◽  
Stephen J. Skinner
Keyword(s):  
Ion Transport ◽  
Transport Properties ◽  
Room Temperature ◽  
Cation Ordering ◽  
Temperature Structure ◽  
Complex Structures ◽  
Ion Conducting ◽  
Oxide Ion

Cation ordering in the modulated La(Nb,W)O4+d phases has been demonstrated and the role of W in inducing occupancy modulation to this phase discussed. This is linked with the oxide ion transport, highlighting that modulated complex structures are viable candidates for ion conducting applications.

scholarly journals Insights into Gasdermin D activation from the crystal structure of its C-terminal domain

2017 ◽  
Author(s):  
Leonie Anton ◽  
Lorenzo Sborgi ◽  
Sebastian Hiller ◽  
Petr Broz ◽  
Timm Maier
Keyword(s):  
Crystal Structure ◽  
Cell Death ◽  
Plasma Membrane ◽  
Biochemical Characterization ◽  
Terminal Domain ◽  
Surface Patches ◽  
Activation Pathways ◽  
Interdomain Interactions

AbstractGasdermin D (GSDMD) is the central executioner of pyroptosis, a proinflammatory type of cell death. GSDMD is activated by the proinflammatory caspase-1 and caspase-11 via proteolytic cleavage in the linker connecting its N-terminal and C-terminal domain (GSDMDNterm, Cterm). The released N-terminal domain is sufficient to form pores in the plasma membrane, resulting in swelling and subsequent rupture of the cell. Here, we report the crystal structure of the autoinhibitory C-terminal domain of GSDMD at 2.04 Å resolution to further analyse determinants of GSDMD activation. GSDMDCterm adopts a compact helical fold unique to gasdermin proteins. Structural analysis and comparison to other gasdermin proteins reveals a conserved key interface for interactions between GSDMDNterm and GSDMDCterm. Variations in two additional surface patches involved in interdomain interactions in full-length gasdermins suggest a role of these regions in modulating activation pathways, in agreement with biochemical characterization of different gasdermins.

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