scholarly journals Scaffold-free tracheal cartilage engineering using roller bottle culture

2021 ◽  
Author(s):  
Imran Sheikh
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Articular Chondrocytes ◽  
Primary Anastomosis ◽  
Roller Bottle ◽  
Cartilage Engineering ◽  
Bovine Articular Chondrocytes ◽  
Tracheal Tissue ◽  
Tracheal Replacement

Resection with primary anastomosis can only repair up to 50% of the adult trachea and up to 30% of the pediatric trachea when damaged. There is a strong clinical need for long-segment tracheal replacements. The goal of this research was to create a seamless, scaffold-free cartilage cylinder for tracheal tissue engineering in vitro. Primary bovine articular chondrocytes were seeded onto tracheal moulds for roller bottle culture and the effect of rotational speed, growth factor supplementation, and chondrocyte layering were investigated. After the 4-week culture period, samples were evaluated biochemically, histologically, and biomechanically. The results indicated that rotation was necessary for full tissue coverage, with slower rotational speeds generating thicker tissue with an improved extracellular matrix, IGF-1 supplementation generating thicker tissue rich in glycosaminoglycans with inferior mechanical properties, and chondrocyte layering producing thinner tissue with increased mechanical properties. Overall, scaffold-free tissue engineering can generate seamless cylindrical cartilage constructs using roller bottle culture for future applications in long-segment tracheal replacement.

scholarly journals Scaffold-free tracheal cartilage engineering using roller bottle culture

2021 ◽  
Author(s):  
Imran Sheikh
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Articular Chondrocytes ◽  
Primary Anastomosis ◽  
Roller Bottle ◽  
Cartilage Engineering ◽  
Bovine Articular Chondrocytes ◽  
Tracheal Tissue ◽  
Tracheal Replacement

Resection with primary anastomosis can only repair up to 50% of the adult trachea and up to 30% of the pediatric trachea when damaged. There is a strong clinical need for long-segment tracheal replacements. The goal of this research was to create a seamless, scaffold-free cartilage cylinder for tracheal tissue engineering in vitro. Primary bovine articular chondrocytes were seeded onto tracheal moulds for roller bottle culture and the effect of rotational speed, growth factor supplementation, and chondrocyte layering were investigated. After the 4-week culture period, samples were evaluated biochemically, histologically, and biomechanically. The results indicated that rotation was necessary for full tissue coverage, with slower rotational speeds generating thicker tissue with an improved extracellular matrix, IGF-1 supplementation generating thicker tissue rich in glycosaminoglycans with inferior mechanical properties, and chondrocyte layering producing thinner tissue with increased mechanical properties. Overall, scaffold-free tissue engineering can generate seamless cylindrical cartilage constructs using roller bottle culture for future applications in long-segment tracheal replacement.

Dexamethasone loaded bilayered 3D tubular scaffold reduces restenosis at the anastomotic site of tracheal replacement: in vitro and in vivo assessments

Nanoscale ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 4846-4858 ◽  
Author(s):  
Sang Jin Lee ◽  
Ji Suk Choi ◽  
Min Rye Eom ◽  
Ha Hyeon Jo ◽  
Il Keun Kwon ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Patient Specific ◽  
Anastomotic Site ◽  
Tubular Scaffold ◽  
Recent Developments ◽  
Tracheal Tissue ◽  
The Creation ◽  
Tracheal Replacement

Despite recent developments in the tracheal tissue engineering field, the creation of a patient specific substitute possessing both appropriate mechanical and biointerfacial properties remains challenging.

scholarly journals Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1184
Author(s):  
Seongwon Lee ◽  
Joohee Choi ◽  
Jina Youn ◽  
Younghun Lee ◽  
Wooyoup Kim ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Chondroitin Sulfate ◽  
Silk Fibroin ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Cell Encapsulation ◽  
Gellan Gum ◽  
Cartilage Tissue

Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.

scholarly journals Synthesis of cartilage matrix by mammalian chondrocytes in vitro. II. Maintenance of collagen and proteoglycan phenotype.

1982 ◽  
Vol 93 (3) ◽  
pp. 751-757 ◽  
Author(s):  
K E Kuettner ◽  
V A Memoli ◽  
B U Pauli ◽  
N C Wrobel ◽  
E J Thonar ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Articular Chondrocytes ◽  
Type Ii Collagen ◽  
Type I ◽  
Type Ii ◽  
Roller Bottle ◽  
The Media ◽  
Bovine Articular Chondrocytes ◽  
Associated Matrix

The in vitro phenotype of bovine articular chondrocytes is described. Chondrocytes plated at high density in roller-bottle and dish cultures were maintained in vitro. The major matrix macromolecules, collagen and proteoglycan, synthesized by these cells were characterized during the course of the culture period. The chondrocytes synthesized mainly Type II collagen, which was found predominantly in the cell-associated matrix. The media contained a mixture of Type II and Type III collagens. Type I collagen was detectable in neither the medium nor the cell-associated matrix. The proteoglycan monomers found in media and cell-associated matrix had the same hydrodynamic sizes as monomers synthesized by cartilage slices or those extracted from adult articular cartilage. The majority of proteoglycans synthesized by the cells were found in high molecular weight aggregates which were readily recovered from the media and were extractable from cell-associated matrix with low ionic strength buffers. The results demonstrate the long-term in vitro phenotypic stability of the bovine articular chondrocytes. The advantages of the in vitro system as a model for studying the effects of external agents, such as drugs and vitamins, are discussed.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

Biomineralization of Electrospun Nano-HA/PHB GTR Membrane

2007 ◽  
Vol 330-332 ◽  
pp. 695-698 ◽  
Author(s):  
Dong Hua Guan ◽  
Chun Peng Huang ◽  
Ji Liu ◽  
Kun Tian ◽  
Lin Niu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Electrospinning Process ◽  
Nanometer Scale ◽  
High Porosity ◽  
The Novel ◽  
Mineral Crystal ◽  
Novel Technology ◽  
Air Jet

Poly 3-hydroxybutyrate (PHB) as a kind of polysaccharides has been proved promising for tissue engineering because of its biocompatibility and biodegradability. But its poor mechanical properties and hydrophilicity limit its application. In order to explore a new useful porch to improve the performance of PHB-based GTR membrane, membrane composed of nano-HA / PHB composite was manufactured through the air/jet electrospinning process which can potentially generate nanometer scale diameter fibers and enlarge surface area of materials while maintaining high porosity. Successively, the biomineralization behavior of the membrane in supersaturated calcification solution (SCS) was studied. The Results of this investigation show that the successfully manufactured porous nano-HA/PHB membrane has high activity in SCS and its ability of inducing the formation of mineral crystal in vitro than that of the unfilled PHB membrane. It can be concluded that the addition of nano-HA and the novel technology could improve the performance of the PHB-based GTR membrane.

Surface Properties and Cell Response of Bioactive Thermally Grown TiO2 Nanofibers

2014 ◽  
Vol 575 ◽  
pp. 219-222
Author(s):  
A.W. Tan ◽  
Belinda Pingguan-Murphy ◽  
Roslina Ahmad ◽  
Sheikh Akbar
Keyword(s):  
Surface Roughness ◽  
Articular Chondrocytes ◽  
Substrate Surface ◽  
Control Sample ◽  
Degree Of Crystallinity ◽  
Crystallographic Structure ◽  
Cartilage Growth ◽  
Bovine Articular Chondrocytes

Titania nanofiber (TiO2 NFs) arrays were fabricated in situ on a Ti-6Al-4V substrate by an oxidation process. Their surface morphology, crystallographic structure, surface roughness and wettability were characterized, as well as their in vitro interaction with bovine articular chondrocytes at different time points. Results showed that TiO2 NFs possessed greater surface roughness, hydrophilicity and degree of crystallinity. The in vitro cell studies revealed that TiO2 NFs substrate triggers enhanced cell adhesion, proliferation and extracellular matrix (ECM) formation compared to the untreated control sample. These results showed that chondrocytes have an affinity to the nanofibrous substrate surface and thus we suggest that such surfaces are suited to be used as an implant designed for cartilage growth.

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