scholarly journals EFECTOS DEL SORBATO DE POTASIO A DIFERENTES CONCENTRACIONES y TIEmPO DE ExPOSICIóN SOBRE EL CICLO CELULAR y EL mATERIAL GENÉTICO EN mERISTEmOS RADICULARES DE AllIuM CEPA l. (CEBOLLA)

2019 ◽  
pp. 71-78
Keyword(s):  
Cell Cycle ◽  
Mitotic Index ◽  
Allium Cepa ◽  
Genetic Material ◽  
Potassium Sorbate ◽  
Control Group ◽  
Root Meristems ◽  
Binucleated Cells ◽  
Allium Cepa L ◽  
Ciclo Celular

EFECTOS DEL SORBATO DE POTASIO A DIFERENTES CONCENTRACIONES y TIEmPO DE ExPOSICIóN SOBRE EL CICLO CELULAR y EL mATERIAL GENÉTICO EN mERISTEmOS RADICULARES DE AllIuM CEPA l. (CEBOLLA) EFFECT OF POTASSIUm SORBATE AT DIFFERENT CONCENTRATIONS AND ExPOSURE TImE ON THE CELL CyCLE AND THE GENETIC mATERIAL IN ROOT mERISTEmS OF AllIuM CEPA l “ONION” Joselyne Quispe, José Saldaña, Tony Verde y Shirley Valderrama Universidad Nacional de Trujillo, Trujillo,Perú DOI: https://doi.org/10.33017/RevECIPeru2010.0011/ RESUMEN Con el propósito de determinar el efecto del Sorbato de Potasio sobre el ciclo celular en meristemos radiculares de Allium cepa L. “cebolla” se establecieron tres grupos experimentales que se expusieron a las soluciones de Sorbato de Potasio en concentraciones de 0.5, 1 y 2 g/L; por 6, 7 y 8 horas cada una y un grupo control. Luego se realizó la respectiva coloración en Orceína acética al 2% de acuerdo a la técnica de Tjio y Levan, observándose con el microscopio compuesto un total de 1500 células meristemáticas en cada uno de los tratamientos. Los resultados aplicando tratamiento después de 8 horas de exposición a la concentración de 2 g/L mostraron una disminución del índice mitótico de 11.26 %, mientras que se incrementó el índice profásico en 95.57 %. Se produjeron también alteraciones como rupturas cromosómicas, puentes anafásicos y células binucleadas. Las pruebas y análisis de varianza, mostraron diferencias significativas entre los diferentes tratamientos, confirmado mediante la prueba de comparación múltiple de promedios de Duncan. Se evidencia un probable efecto genotóxico en el material genético por el Sorbato de Potasio y una alteración en el ciclo celular de los meristemos de A. cepa L.; así mismo, se comprueba la función de biosensor de A. cepa L. en el estudio de sustancias que puedan afectar el ciclo celular. Palabras clave: Allium cepa, Sorbato de Potasio, Índice Mitótico, Alteración del material genético, Puentes anafásicos, Rupturas cromosómicas, Células binucleadas. ABSTRACT In order to determine the effect of Potassium Sorbate on the cell cycle in root meristems of Allium cepa L. “onion” set three experimental groups were exposed to solutions of Potassium Sorbate at concentrations of 0.5, 1 and 2g /l, for 6, 7 and 8 hours each and a control group. Later the respective color in 2% Acetic orcein according to the technique of Tjio and Levan, observed with the compound microscope a total of 1500 meristematic cells in each of the treatments. The appropriate treatment results after 8 hours of exposure to the concentration of 2 g / L showed a decrease in mitotic index of 11.26%, while it increased the rate at 95.57% prophasic index. There were also alterations as chromosome breaks, anaphasic bridges and binucleated cells. The tests and analysis of variance showed significant differences between the different treatments, as confirmed by multiple comparison test of Duncan averages. It shows a probable genotoxic effect in the genetic material by Potassium Sorbate and an alteration in the cell cycle of meristems of A. cepa L., likewise, is found biosensor function of A. cepa L. in the study of substances that may affect the cell cycle. Keywords: Allium cepa, Potassium Sorbate, Mitotic index, Abnormal genetic material, Chromosomal breaks, Anafasic bridges, Binucleated cells.

scholarly journals Cytogenetic effects of insecticide Lamda cyhalothrin on meristemic cells of Allium cepa L.

2008 ◽  
Vol 5 (2) ◽  
pp. 166-172
Author(s):  
Baghdad Science Journal
Keyword(s):  
Mitotic Index ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Cytogenetic Effects ◽  
Binucleated Cells ◽  
Allium Cepa L ◽  
Spindle Fibers

The effect of insecticide lamda cyhalothrin on Allium cepa L.root meristem were studied cytogenetically .Using three concentrations of the insecticide 5%, 7.5%, 10% at 6,12,24hours treatments . The insecticide significantly reduced the mitotic index at all concentrations. Moreover showed its ability to induced C – metaphase in 10% .The effects were also caused chromosome aberration like stickness in anaphase, telophase, binucleated cells, Micronuclei formation. These alternations indicating that this insecticide was effective in producing disturbance of spindle fibers.

scholarly journals Citogenotoxicidade de extratos aquosos de Erythrina fusca Lour. sobre o ciclo celular de Allium cepa L / Citotoxity and genotoxity of aqueous extracts of Erythrina fusca Lour. about the cell cycle of Allium cepa L

2021 ◽  
Vol 7 (10) ◽  
pp. 99270-99285
Author(s):  
Rosieli Barboza Bispo ◽  
Elisa dos Santos Cardoso ◽  
Nilo Leal Sander ◽  
Joari Costa De Arruda ◽  
Alex Souza Rodrigues ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Aqueous Extracts ◽  
Allium Cepa L ◽  
Ciclo Celular

scholarly journals Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L.

2014 ◽  
Vol 86 (3) ◽  
pp. 1147-1150 ◽  
Author(s):  
LOURRAN P. LACERDA ◽  
GEIZ MALAQUIAS ◽  
ANA PAULA PERON
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Test System ◽  
Control Group ◽  
Aqueous Extracts ◽  
Root Cells ◽  
Antiproliferative Action ◽  
Allium Cepa L ◽  
Meristematic Root ◽  
Number Of Cells

In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be cytotoxic.

scholarly journals EFEITO CITOTÓXICO DE EXTRATOS AQUOSOS DE ARTEMISIA ABSINTHIUM L. SOBRE O CICLO CELULAR DE ALLIUM CEPA L. / CYTOTOXIC EFFECT OF AQUEOUS EXTRACTS OF ARTEMISIA ABSINTHIUM L. ON THE CELL CYCLE OF ALLIUM CEPA L.

2020 ◽  
Vol 6 (9) ◽  
pp. 64893-64906
Author(s):  
Daniele Paula Maltezo ◽  
Alex Souza Rodrigues ◽  
Uéliton Alves de Oliveira ◽  
Larissa Lemes dos Santos ◽  
Kelli Évelin Müller Zortéa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Allium Cepa ◽  
Cytotoxic Effect ◽  
Aqueous Extracts ◽  
Artemisia Absinthium ◽  
Allium Cepa L ◽  
Ciclo Celular

A Comparison of Cytotoxic and Genotoxic Damages Induced by 254 Nm and 365 Nm Radiation: Allium Cepa L. Model

2021 ◽  
Author(s):  
Kültiğin ÇAVUŞOĞLU ◽  
Tuğçe Kalefetoğlu Macar ◽  
Oksal MACAR ◽  
Dilek ÇAVUŞOĞLU ◽  
Emine YALÇIN
Keyword(s):  
Oxidative Stress ◽  
Allium Cepa ◽  
Germination Percentage ◽  
The Other ◽  
Uv Exposure ◽  
Control Group ◽  
Total Weight Gain ◽  
Allium Cepa L ◽  
Living Organisms ◽  
Uv C

Abstract Living organisms are increasingly exposed to ultraviolet (UV) rays of solar radiation, both due to the thinning of the ozone layer and the widespread uses in sterilization processes. The present study was conducted with the purpose of evaluating the damages of UV-A and UV-C radiations in Allium cepa L. roots. Three groups were formed from Allium bulbs, one of which was the control group. One of the other groups was exposed to 254 nm (UV-C) and the other to 365 nm (UV-A) UV. Growth retardation effect of UV was investigated with respect to germination percentage, total weight gain and root elongation, while genotoxicity arisen from UV exposure analyzed using mitotic index (MI) and chromosomal aberrations (CAs) including micronucleus (MN) frequency. Oxidative stress due to UV application was investigated based on the accumulation of malondialdehyde (MDA) and the total activities of superoxide dismutase (SOD) and catalase (CAT) enzymes. Also, meristematic integrity of the UV treated roots was controlled. UV treatments caused significant changes in all parameters compared to the control, but all effects were much more prominent in 254 nm UV-exposed group. This study clearly revealed that UV exposure triggered growth inhibition, genotoxicity, oxidative stress and meristematic cell damages in A. cepa roots depending on the wavelength.

Extracts affecting mitotic division in root-tip meristematic cells

Biologia ◽  
2008 ◽  
Vol 63 (5) ◽  
Author(s):  
Greice Lubini ◽  
Juliana Fachinetto ◽  
Haywood Laughinghouse ◽  
Juçara Paranhos ◽  
Antonio Silva ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Mitotic Index ◽  
Mitotic Division ◽  
Root Tip ◽  
Distilled Water ◽  
Female Reproductive System ◽  
Genotoxic Effects ◽  
Antiproliferative Effects ◽  
Ethanol Acetic Acid ◽  
Allium Cepa L

AbstractSome species of Psychotria (Rubiaceae) are important in herbal medicine, where their extracts are used internally for infections of the female reproductive system, bronchitis, gastrointestinal disturbances, skin irritations, tumors, ulcers, and eye disturbances. The antiproliferative and genotoxic effects of Psychotria myriantha Mull. Arg. and P. leiocarpa Cham. et Schlecht infusions on the Allium cepa L. cell cycle were evaluated. The teas were prepared by infusing the leaves in distilled water, in two concentrations: 1.13 mg/mL and 6.78 mg/mL. Three groups of four bulbs were used for each Psychotria species. After the bulbs rooted in distilled water, they were transferred to the teas for 24 hours, except for the control that stayed in water. The rootlets were then collected, fixed in ethanol-acetic acid (3:1) for 24 hours, and stored in 70% ethanol. For each group of bulbs, 4000 cells were analyzed, calculating the mitotic indexes, submitting them to statistic analysis, using the χ 2 test (p = 0.05). The results showed a decrease in mitotic index with an increase in tea concentration in both species. In P. leiocarpa, the mitotic index values differed significantly between the control and concentration of 6.78 mg/mL (χ 2 = 9.863). For P. myriantha, the values referring to the mitotic index differed greatly between the control and the treatments (χ 2 = 124.8). With this study, it was determined that P. myriantha and P. leiocarpa infusions possess antiproliferative effects on the A. cepa cell cycle, and teas of P. myriantha also have genotoxic activity.

scholarly journals Appraisal of Cell Cycle Delay and Cytotoxicity Inducing Potential of 3-epicaryoptin in Allium Cepa L.

2021 ◽  
Author(s):  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Cell Cycle ◽  
Medicinal Plant ◽  
Traditional Medicine ◽  
Allium Cepa ◽  
Apical Meristem ◽  
Root Apical Meristem ◽  
Cytotoxic Effects ◽  
Cell Cycle Delay ◽  
Allium Cepa L

Diterpenoid 3-epicaryoptin (C26H36O9) is abundant in leaves of Clerodendrum inerme, a traditionally used medicinal plant, having insect antifeedant activities. Here, we aim to explore its cell cycle delay and cytotoxic effects in Allium cepa root apical meristem cells. Allium cepa roots were treated with 3-epicaryoptin and colchicine for 2, 4, 4+16 h (4 h treatment followed by 16 h recovery) and the induced cell cycle delay and cytotoxic effects were analyzed. The highest metaphase cells frequency for 3-epicaryoptin and colchicine treated samples were found to be 66.2% and 82.35% respectively at 4 h treatment. Treatment of 3-epicaryoptin and colchicine increased the aberrant cells %, CA, MN, c-metaphase, and PP cells. Therefore, indiscriminate use of C. inerme in traditional medicine should be restricted and the cell cycle delay and cytotoxicity inducing effects of 3-epicaryoptin needs further exploration for its cancer chemotherapeutic applications.

scholarly journals Effect of Ferrous Gluconate on Chromosomal Abnormality Index of Allium Cepa Root Tip

2021 ◽  
Vol 9 (4) ◽  
pp. 755-758
Author(s):  
Nergis Kaya
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Abnormality ◽  
Genotoxic Effect ◽  
Root Tip ◽  
Control Group ◽  
Root Tips ◽  
Ferrous Gluconate ◽  
Allium Cepa L ◽  
Abnormality Index

In completed research, ferrous gluconate -a food additive- used to preserve black color to prevent discoloration during storage in ripe black olives, and Allium cepa L. species. A. cepa L. roots were treated with different doses of ferrous gluconate. The effective concentration EC50 (0.068 g/l) was determined. A. cepa root tips were treated with EC50/2 (0.034 g/l), EC50 (0.068 g/l), 2XEC50 (0.136 g/l) dose for 24, 48, 72 hours, and afterward, the root tips were prepared for observation under the light microscope according to the method of preparing mitotic preparation. Chromosomal abnormality index (CAI) and genotoxic effect of ferrous gluconate in A. cepa root tip cells were determined. Repeated measurement ANOVA and TUKEY multiple comparison tests were used to investigate the effect of time and dose together on genotoxicity. C-mitosis, polyploidy, polar shifting in anaphase, polar shifting in telophase, equatorial plate shifting, laggard chromosome was observed by microscope. The highest CAI (70.16±4.85) was observed at 72h for 2XEC50 dose. Chromosomal aberration is also observed in control group. While the most common chromosomal aberration is determined as C-mitosis; The least observed chromosomal aberration is determined as polyploidy. Research results revealed that ferrous gluconate has a genotoxic effect on the root tip of A. cepa.

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