PENGGUNAAN ALGA GEL (NATACLHORELLA) DALAM LARVA REARING UNTUK MENINGKATKAN SURVIVAL RATE BENIH KERAPU SUNU (Plectropomus leopardus)

Kondisi Pulau Ambon yang sering hujan memberikan dampak yang kurang menguntungkan bagi kegiatan pembenihan di BBL-Ambon. Hal ini disebabkan perkembangan fitoplankton yang merupakan makanan alami bagi pertumbuhan larva dan benih tidak optimal dan mengalami kematian. Keterbatasan ketersediaan fitoplankton dapat menyebabkan kegagalan dalam kegiatan pembenihan secara keseluruhan. Ini karena fungsi dari fitoplankton sangat vital bagi kelangsungan hidup larva. Fitoplankton selain berfungsi sebagai makanan zooplankton juga menstabilkan kualitas air media pemeliharaan. Natachlorella merupakan salah produk dari fitoplankton yang dipadatkan dari jenis Chlorella dalam bentuk gel. Penggunaannya kini semakin berkembang di instalasi pembenihan pada saat kondisi musim hujan dan intensitas matahari rendah.Penggunaan natachlorela dalam pembenihan kerapu sunu belum pernah dilakukan, ini karena perkembangan pembenihan kerapu sunu yang belum berkembang optimal. Untuk itu dalam kegiatan pembenihan kerapu sunu dalam kondisi hujan BBL-Ambon menggunakan natachlorela yang dilarutkan dalam media pemeliharaan.Kegiatan ini dilakukan selama 3 siklus pemijahan dari bulan Oktober – Desember 2012. Pemberian plankton (natadechlorella sp) dilakukan pada umur larva D2 sebanyak 1 kali sehari dan diberikan pada waktu pagi hari. Ketika larva berumur D3 - D25 plankton diberikan 2 kali sehari (pagi dan sore), dengan dosisi 20 ml/10 ton air laut.Hasil yang diperoleh dari 3 bulan siklus pemijahan adalah 1020 ekor benih kerapu sunu (P. leopardus) ukuran 3 cm

PERTUMBUHAN DAN SINTASAN LARVA DAN BENIH IKAN KERAPU SUNU Plectropomus leopardus TURUNAN KETIGA (F-3) DARI INDUK HASIL SELEKSI

Jurnal Riset Akuakultur ◽

10.15578/jra.12.2.2017.131-137 ◽

2017 ◽

Vol 12 (2) ◽

pp. 131

Author(s):

Ahmad Muzaki ◽

Sari Budi Moria Sembiring ◽

Ida Komang Wardana ◽

Haryanti Haryanti

Keyword(s):

Survival Rate ◽

Growth Trait ◽

Fast Growth ◽

Third Generation ◽

Growth And Survival ◽

The Third ◽

Coral Trout ◽

Single Allele ◽

Plectropomus Leopardus ◽

Eggs And Larvae

Pertumbuhan dan sintasan larva dan benih kerapu sunu turunan ketiga (F-3) dari induk turunan kedua (F-2) hasil seleksi telah diamati di Balai Penelitian dan Pengembangan Budidaya Laut, Gondol. Induk F-2 diseleksi menggunakan marka indikator tumbuh cepat PL-03 (alel tunggal berukuran 370 bp). Penelitian ini bertujuan untuk mengevaluasi keragaan pertumbuhan dan sintasan larva dan benih kerapu sunu F-3 serta pewarisan sifat tumbuh cepat dari induk kerapu sunu (F-2) kepada benih kerapu sunu F-3. Pembenihan menggunakan induk kerapu sunu F-2 dengan sifat tumbuh cepat (membawa marka PL-03) dan tanpa sifat tumbuh cepat. Telur dan larva yang dihasilkan dipelihara sebagai turunan F-3 hingga berukuran benih dan dibesarkan selama 10 bulan pemeliharaan. Hasil pengamatan sintasan larva kerapu sunu F-3 dari induk membawa marka PL-03 adalah sebesar 10,6% ± 9,1%; sedangkan larva F-3 dari induk yang tidak membawa sifat tumbuh cepat hanya sebesar 2,7% ± 1,7%. Benih F-3 dari induk membawa marka PL-03 dapat dipanen saat mencapai ukuran 2,5-3,0 cm pada umur 38 ± 2 hari, sedangkan benih F-3 dari induk tanpa marka PL-03 pada umur 40 ± 1 hari. Selanjutnya benih kerapu sunu F-3 dari induk yang mempunyai marka PL-03 yang dipelihara selama 10 bulan memiliki laju pertumbuhan harian lebih baik (3,11%) dibandingkan benih kerapu sunu F-3 tanpa marka PL-03 (3,01%). Sintasan benih kerapu sunu F-3 dengan marka PL-03 tidak berbeda nyata dengan yang tidak mempunyai marka PL-03. Persentase benih F-3 turunan dari induk F-2 dengan sifat tumbuh cepat yang membawa marka PL-03 sebesar 45%.The growth and survival rate of the third generation (F-3) of coral trout grouper larvae and fry from the genetically selected broodstock were observed at the Institute for Mariculture Research and Development, Gondol. The F-2 broodstock were selected using the fast-growing marker indicator, PL-03 (single allele of 370 bp in size). The aim of this study was to determine the growth performance and survival rate of larvae and fry of F-3 coral trout groupers, as well as inheritance of fast growth trait from the F-2 broodstock to the F-3. The eggs and larvae of F-3 produced from F-2 broodstock with fast growth trait (having PL-03 marker) and without fast growth trait were reared to reach fry stage for 10 months. The results showed that the survival rate of the F-3 larvae from broodstock with PL-03 marker was 10.6% ± 9.1%, while F-3 larvae from the broodstock without fast growth trait only reached 2.7% ± 1.7%. Fry (F-3) with PL-03 marker reached the optimum harvest size of 2.5-3.0 cm at 38 ± 2 days old, while the F-3 fry of broodstock without PL-03 marker took relatively longer to reach that size (40 ± 1 days age). The F-3 from the broodstock with PL-03 marker reared for 10 months had better growth rate (3.11%) compared to F-3 fry without PL-03 marker (3.01%). The survival rate of F-3 fry with PL-03 marker was not significantly different to F-3 fry without PL-03 marker. The percentage of F-3 fry from the broodstock of F-2 with fast growth trait was 45%.

OPTIMIZATION OF THE FORMULATED FEEDING IN JUVENILE CORAL TROUT, Plectropomus leopardus IN CONTROLLED TANK

10.28930/jitkt.v8i2.15823 ◽

2017 ◽

Vol 8 (2) ◽

pp. 585

Author(s):

Anak Agung Alit ◽

Ketut Maha Setiawati ◽

Tony Setiadharma

Keyword(s):

Survival Rate ◽

Local Time ◽

Body Length ◽

Artificial Feeding ◽

Feed Conversion ◽

Additional Food ◽

Coral Trout ◽

Juvenile Coral ◽

Randomized Design ◽

A coral trout grouper is the fishery commodities to generate foreign exchange through exports, to date cultivation continued. The purpose is to obtain data and information on the dose of the right artificial feeding and can increase the survival rate of the optimum seed nursery in the juvenile coral trout in controlled tank. Research has been conducted at the hatchery and the Institute for Mariculture Reasearch and Development Gondol, Bali. Containers experiment is used is a fiber with a size of 1 m³ up to 12 containers. test animals used were seeds of coral trout, Plectropomus leopardus with initial body length on average 3 cm with each treatment density of 100 fish/m³. Coral trout, Plectropomus leopardus seed kept in fibers. This type of feed given in the form of commercial pellet feed with a protein content of 48%, and additional food such as shrimp Mysid (jembret) for 10 days. The frequency of feeding 3 times a day (morning 8:00. local time, lunch 13:00 local time, and afternoon 17:00 local time). Treatment feeding attempted is the treatment of A = 5% dose of artificial feeding, B = 10% dose of artificial feeding, C = 15% dose of artificial feeding, and D = 20% dose of artificial feeding. The study used a completely randomized design with 4 treatments, each treatment was repeated 3 times. Data were analyzed by analysis of variance. If the variance is different then conducted a further test using the smallest real difference test (BNT). The results showed that significantly different (P <0.05) on survival, feed conversion, weight gain and body length of coral trout. The highest survival rate in treatment C = 15% dose artificial feeding is. = 90.83 ± 1.80%, followed by treatment D, B, and A.

DIFFERENT PERCENTAGE OF WATER EXCHANGE ON GROWTH OF CORAL TROUT GROUPER FINGERLING (Plectropomus leopardus)

10.29244/jitkt.v2i2.7849 ◽

2010 ◽

Vol 2 (2) ◽

Author(s):

Titiek Aslianti

Keyword(s):

Body Weight ◽

Survival Rate ◽

Water Exchange ◽

Feeding Frequency ◽

Growth And Survival ◽

Coral Trout ◽

Survival And Growth ◽

Plectropomus Leopardus ◽

The One ◽

Net Cages

Coral trout grouper (Plectropomus leopardus) is the prospective aquaculture commodities with a high demand. Fingerling culture is the one of shortly rearing time methods for grow out in net-cages in order to produce continuously. Two hundred fishes with initial total length 16.6±0.5 cm and 72.2±7.6 g of body weight were stocked in each of three of concrete tanks of 4m3 seawater. Water replace as a treatment was applied i.e. A (200%/day), B (300%/day) and C (400%/day).respectively. Feeding frequency of pellet was done twice a day of about 3 - 5% of body weight. Survival and growth of fishes were monitored during two-month period. The result showed that the different percentage of water replace had a significant role in increasing the growth and survival rate of the fishes. Water replaced of 400% daily (treatment C) gave the best survival rate (97%) and growth performance (TL 21.61±0,54 cm; BW 156.84±1.05 g). In contrast, the treatment B (300%/day) and A (200%/day) resulted in a lower survival and growth , i.e. (SR 95.5%; TL 19.51±0.52 cm; BW 140.96±0.08 g) and A (SR 93%; TL 19.08±0.30 cm; BW 132.2±2.65 g)., respectively.Keywords: Coral trout grouper, growth, survival rate, water exchange

GROWTH AND BLOOD PROFILE OF CORAL TROUT GROUPER Plectropomus leopardus SEEDS IN DIFFERENT SALINITIES

10.29244/jitkt.v9i2.19290 ◽

2018 ◽

Vol 9 (2) ◽

pp. 557-568

Author(s):

Ketut Maha Setiawati ◽

Ketut Mahardika ◽

A. A. Ketut Alit ◽

Daniar Kusumawati ◽

Indah Mastuti

Keyword(s):

Body Weight ◽

Survival Rate ◽

Sea Water ◽

Feed Conversion ◽

Blood Profile ◽

Total Length ◽

Coral Trout ◽

Water Replacement ◽

Plectropomus Leopardus ◽

Optimum Salinity

The information about the optimum and tolerable salinity to support in the success of coral trout grouper’s seed culture is still limited until now. The aim of this study was to understand the effect of different salinity on growth, survival, feed conversion (FCR) and the profile of blood (hematocrit and hemoglobin) of coral trout grouper seed. The seed with total length 6.0 ± 0.5 cm and body weight 3.2±0.3 g were used as tested animals. Five different salinity treatments, namely: 10±1 ppt (A), 16±1 ppt (B), 22±1 ppt (C), 28±1 ppt (D), and the sea water (34 ppt) as a control (E) were undertaken in this study, with three replications for each treatment. Salinity values were reach by adding freshwater into the tested seawater. Water changing system in this study was performed in recirculation process, with 25% of water replacement every 7 days. The result of this study showed that the survival rate values were 100% for all treatments, indicating that coral trout grouper seed can be cultured in the range of salinity between 10 to 34 ppt. The highest total length and body weight were recorded from treatment C (22 ppt), as well as noted to gain the lowest FCR. Correspondingly, the seed in treatment C also founded to have in the greatest amount of hematocrit and hemoglobin. Optimum salinity for rearing of coral trout grouper seeds with total length 6 cm was 22 ppt. Keywords: salinity, growth, survival rate, feed conversion, blood profile

DIFFERENT PERCENTAGE OF WATER EXCHANGE ON GROWTH OF CORAL TROUT GROUPER FINGERLING (Plectropomus leopardus)

10.28930/jitkt.v2i2.7849 ◽

2010 ◽

Vol 2 (2) ◽

Author(s):

Titiek Aslianti

Keyword(s):

Body Weight ◽

Survival Rate ◽

Water Exchange ◽

Feeding Frequency ◽

Growth And Survival ◽

Coral Trout ◽

Survival And Growth ◽

Plectropomus Leopardus ◽

The One ◽

Net Cages

Coral trout grouper (Plectropomus leopardus) is the prospective aquaculture commodities with a high demand. Fingerling culture is the one of shortly rearing time methods for grow out in net-cages in order to produce continuously. Two hundred fishes with initial total length 16.6±0.5 cm and 72.2±7.6 g of body weight were stocked in each of three of concrete tanks of 4m3 seawater. Water replace as a treatment was applied i.e. A (200%/day), B (300%/day) and C (400%/day).respectively. Feeding frequency of pellet was done twice a day of about 3 - 5% of body weight. Survival and growth of fishes were monitored during two-month period. The result showed that the different percentage of water replace had a significant role in increasing the growth and survival rate of the fishes. Water replaced of 400% daily (treatment C) gave the best survival rate (97%) and growth performance (TL 21.61±0,54 cm; BW 156.84±1.05 g). In contrast, the treatment B (300%/day) and A (200%/day) resulted in a lower survival and growth , i.e. (SR 95.5%; TL 19.51±0.52 cm; BW 140.96±0.08 g) and A (SR 93%; TL 19.08±0.30 cm; BW 132.2±2.65 g)., respectively.Keywords: Coral trout grouper, growth, survival rate, water exchange

OPTIMIZATION OF THE FORMULATED FEEDING IN JUVENILE CORAL TROUT, Plectropomus leopardus IN CONTROLLED TANK

10.29244/jitkt.v8i2.15823 ◽

2017 ◽

Vol 8 (2) ◽

pp. 585-594

Author(s):

Anak Agung Alit ◽

Ketut Maha Setiawati ◽

Tony Setiadharma

Keyword(s):

Survival Rate ◽

Local Time ◽

Body Length ◽

Artificial Feeding ◽

Feed Conversion ◽

Additional Food ◽

Coral Trout ◽

Juvenile Coral ◽

Randomized Design ◽

A coral trout grouper is the fishery commodities to generate foreign exchange through exports, to date cultivation continued. The purpose is to obtain data and information on the dose of the right artificial feeding and can increase the survival rate of the optimum seed nursery in the juvenile coral trout in controlled tank. Research has been conducted at the hatchery and the Institute for Mariculture Reasearch and Development Gondol, Bali. Containers experiment is used is a fiber with a size of 1 m³ up to 12 containers. test animals used were seeds of coral trout, Plectropomus leopardus with initial body length on average 3 cm with each treatment density of 100 fish/m³. Coral trout, Plectropomus leopardus seed kept in fibers. This type of feed given in the form of commercial pellet feed with a protein content of 48%, and additional food such as shrimp Mysid (jembret) for 10 days. The frequency of feeding 3 times a day (morning 8:00. local time, lunch 13:00 local time, and afternoon 17:00 local time). Treatment feeding attempted is the treatment of A = 5% dose of artificial feeding, B = 10% dose of artificial feeding, C = 15% dose of artificial feeding, and D = 20% dose of artificial feeding. The study used a completely randomized design with 4 treatments, each treatment was repeated 3 times. Data were analyzed by analysis of variance. If the variance is different then conducted a further test using the smallest real difference test (BNT). The results showed that significantly different (P <0.05) on survival, feed conversion, weight gain and body length of coral trout. The highest survival rate in treatment C = 15% dose artificial feeding is. = 90.83 ± 1.80%, followed by treatment D, B, and A.

PENINGKATAN SINTASAN LARVA IKAN KERAPU SUNU (Plectropomus leopardus) MELALUI MANAJEMEN PEMELIHARAAN YANG SESUAI

BERITA BIOLOGI ◽

10.14203/beritabiologi.v18i1.2951 ◽

2019 ◽

Vol 18 (1) ◽

Author(s):

Daniar Kusumawati ◽

Yasmina Nirmala Asih ◽

Ketut Maha Seti

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Larval Development ◽

Early Stage ◽

Larval Rearing ◽

Total Production ◽

Coral Trout ◽

Standard Management ◽

Plectropomus Leopardus ◽

Increase Survival Rate

Survival rate of coral trout seed produced from aquaculture was relatively low. In 2015, high mortality was found at the early stage of larval development (<D10), approximately 64.37 % of the total production. There has been no standard management for coral trout (Plectropomus leopardus) larval rearing, which was used to adopt management for grouper (Epinephelus spp.) larval rearing. This study was to apply suitable management for coral trout larval rearing into aimed to decrase mortality at early stage larval development and to increase survival rate. There were two different managements in this study: A. applying the results of previous research of coral trout larval rearing from 2004 to 2015, and B. using standard management for grouper larval rearing. The results showed that management A was more suitable management for coral trout larval rearing compared to management B. Management A resulted in the increasing of survival rate by five times (p=0.0056) and of growth rate at early stage by 1.15 times (p=0.2338) higher than those of management B.

APLIKASI VAKSIN BIVALEN (VNN dan GSDIV) PADA PEMELIHARAAN LARVA Ikan Kerapu Sunu, Plectropomus leopardus

Jurnal Riset Akuakultur ◽

10.15578/jra.13.4.2018.337-346 ◽

2019 ◽

Vol 13 (4) ◽

pp. 337

Author(s):

Ketut Mahardika ◽

Indah Mastuti ◽

Sudewi Sudewi ◽

Yasmina Nirmala Asih ◽

Ahmad Muzaki ◽

...

Keyword(s):

Survival Rate ◽

Challenge Test ◽

Sea Bass ◽

Mhc I ◽

Natural Diet ◽

Growth And Survival ◽

Bivalent Vaccine ◽

Coral Trout ◽

Viral Nervous Necrosis ◽

Beta-nodavirus sebagai agen penyebab VNN (virus nervous necrosis) dan infeksi GSDIV (grouper sleepy disease iridovirus, isolat dari genus Megalocytivirus) merupakan penyakit yang menyebabkan mortalitas yang tinggi pada larva dan juvenil ikan kerapu dan kakap di Indonesia. Pencegahan infeksi virus tersebut menjadi prioritas utama dalam budidaya ikan tersebut. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas vaksin bivalen dalam mencegah infeksi virus VNN dan GSDIV pada pemeliharaan larva ikan kerapu sunu, Plectropomus leopardus. Sebanyak 5 mL vaksin bivalen (kombinasi antara vaksin protein rekombinan VNN dan GSDIV dengan rasio 1:1 v/v) di bio-enkapsulasi ke dalam 30 liter pakan alami Rotifera dan Artemia (2 x 104 individu/mL). Aplikasi vaksin pada larva ikan kerapu sunu dilakukan melalui pakan alami Rotifera dari umur 5-24 hari dan Artemia dari umur 25-50 hari. Hasil penelitian menunjukkan bahwa vaksin bivalen tidak berpengaruh terhadap pertumbuhan dan sintasan larva kerapu sunu (panjang: 1,8-2,2 cm dan sintasan: 1,05%-4,07%). Hasil uji tantang dengan VNN dan GSDIV menunjukkan bahwa vaksin tersebut dapat menginduksi gen imunitas larva (MHC-I).Beta-nodavirus as the causative agent of VNN (viral nervous necrosis) and GSDIV infection (grouper sleepy disease iridovirus, isolate from the genus Megalocytivirus) has caused high mortality of cultured grouper and sea bass larvae and juvenile in Indonesia. The prevention of this virus infection on grouper and sea bass culture has become one of the national priority. The purpose of this research was to study the effectiveness of the bivalent vaccine in preventing VNN and GSDIV infections to seed of coral trout grouper, Plectropomus leopardus reared in hatchery. Applications of bivalent vaccine (a combination of protein recombinant VNN and GSDIV vaccine with a ratio of 1:1 v/v) were done by bio-encapsulation using the fish natural diet, Rotifera and Artemia, with a dose of 5 mL vaccine in 30 liters of natural diet (2 x 104 ind./mL). Vaccines were given once a day from the larval age of 5-24 days after hatching using Rotifera and 25-50 day after hatching using Artemia. The results showed that the bivalent vaccine did not influence the growth and survival rate of coral trout grouper larvae (ranged of total length: 1.8-2.2 cm and survival rate: 1.05%-4.07%). The challenge test with VNN and GSDIV revealed that the vaccine had positively induced gene related immunity of larvae MHC-I.