Optimization Of Microwave-Assisted Extraction Of Flavonoids From Binahong (Anredera cordifolia) Leaves Using Respon Surface Methodology

Binahong (Anredera scandens (L.) Moq.) leaves are one of the plants that empirically can be used for wound healing and contain flavonoids which have antibacterial and antioxidant activities. Determination of the optimum conditions of the Binahong leaf extraction process needs to be done to ensure the extraction quality of the Binahong leaves associated with the given activity. Microwave Assisted Extraction (MAE) is an extraction that utilizes microwave radiation to heat the solvent quickly and efficiently. This study aims to determine the most effective binahong leaf extraction conditions that produce optimal levels of flavonoids and antioxidant activity. The extraction process was carried out using the Microwave assisted extraction (MAE) method. Determination of optimum conditions is done based on Response Surface Method (RSM) with variable ethanol concentration (70%, 80% and 90%), extraction time (4, 12 and 20 minutes) and power (450, 600, and 800 watts) using Box- Behnken Design (BBD) with Design Expert 7.0 software. The extract quality parameters measured were total flavonoid levels and antioxidant activity. The results showed the optimum conditions with the BBD method were obtained at an ethanol concentration of 81.49%, extraction time of 13.84 minutes, and power of 626.19 watts with flavonoid levels of 3.8561% and antioxidant activity (IC50) of 95.51834 ppm with active categories.

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Simultaneous Optimization of Microwave-Assisted Extraction of Phenolic Compounds and Antioxidant Activity of Avocado (Persea americana Mill.) Seeds Using Response Surface Methodology

Journal of Analytical Methods in Chemistry ◽

10.1155/2020/7541927 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Alexander Weremfo ◽

Felix Adulley ◽

Martin Adarkwah-Yiadom

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Phenolic Compounds ◽

Response Surface ◽

Microwave Power ◽

Ethanol Concentration ◽

Extraction Time ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction

This study was designed to optimize three microwave-assisted extraction (MAE) parameters (ethanol concentration, microwave power, and extraction time) of total phenolics, total flavonoids, and antioxidant activity of avocado seeds using response surface methodology (RSM). The predicted quadratic models were highly significant (p<0.001) for the responses studied. The extraction of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity was significantly (p<0.05) influenced by both microwave power and extraction time. The optimal conditions for simultaneous extraction of phenolic compounds and antioxidant activity were ethanol concentration of 58.3% (v/v), microwave power of 400 W, and extraction time of 4.8 min. Under these conditions, the experimental results agreed with the predicted values. MAE revealed clear advantages over the conventional solvent extraction (CSE) in terms of high extraction efficiency and antioxidant activity within the shortest extraction time. Furthermore, high-performance liquid chromatography (HPLC) analysis of optimized extract revealed the presence of 10 phenolic compounds, with rutin, catechin, and syringic acid being the dominant compounds. Consequently, this optimized MAE method has demonstrated a potential application for efficient extraction of polyphenolic antioxidants from avocado seeds in the nutraceutical industries.

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PENGARUH WAKTU EKSTRAKSI DAN KONSENTRASI ETANOL MENGGUNAKAN METODE MICROWAVE ASSISTED EXTRACTION (MAE) TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK RAMBUT JAGUNG (Zea mays L.)

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2019.v08.i01.p11 ◽

2019 ◽

Vol 8 (1) ◽

pp. 94

Author(s):

Yessica Kristanti ◽

I Wayan Rai Widarta ◽

I Dewa Gede Mayun Permana

Keyword(s):

Antioxidant Activity ◽

Ethanol Concentration ◽

Extraction Time ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Corn Silk ◽

Assisted Extraction ◽

Ic50 Value ◽

Two Factors ◽

Determine Effect

This research was conducted to determine effect of the extraction time and ethanol concentration on antioxidant activity of corn silk extract and to obtain extraction time and ethanol concentration using Microwave Assisted Extraction (MAE) that can produce extract with the highest antioxidant activity. The experimental design used in this research was a completely randomize design, which consisted of two factors. The fisrt factor was the concentration of ethanol which consisted of 5 level that namely ethanol 30%, 45%, 60%, 75%, and 90%. The second factor was the time of extraction which consisted of 3 level namely 8, 13 and 18 minutes. Data were analyzed with analysis of variance that followed by Duncan test. The result showed that the best treatment of corn silk extract is extraction by ethanol 90% for 18 minutes using MAE which the highest resulted rendemen was 24.54%, total phenol was 159.65 mg GAE/g extract, total flavonoid was 149 mgQE/g extract, antioxidant activity was 70.18%, and the IC50 value 64.806 ppm.

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Optimization of the Microwave-Assisted Extraction Process for Polysaccharides in Himematsutake (Agaricus blazei Murrill) and Evaluation of Their Antioxidant Activities

Food Science and Technology Research ◽

10.3136/fstr.17.461 ◽

2011 ◽

Vol 17 (6) ◽

pp. 461-470 ◽

Cited By ~ 12

Author(s):

Zuofa ZHANG ◽

Guoying LV ◽

Huijuan PAN ◽

Liangen SHI ◽

Leifa FAN

Keyword(s):

Antioxidant Activities ◽

Extraction Process ◽

Microwave Assisted Extraction ◽

Agaricus Blazei ◽

Microwave Assisted ◽

Assisted Extraction

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DETERMINATION OF CHLORPYRIFOS IN LETTUCE (LACTUCA SATIVA L.) BY MICROWAVE-ASSISTED EXTRACTION COUPLED WITH HEADSPACE SINGLE DROP MICROEXTRACTION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.16849 ◽

2017 ◽

Vol 9 (5) ◽

pp. 248

Author(s):

Naruephon Watthanaphap ◽

Surapol Natakankitkul ◽

Nuchnart Jonglaekha

Keyword(s):

Ph Value ◽

Limit Of Detection ◽

Extraction Time ◽

Microwave Assisted Extraction ◽

Optimum Conditions ◽

Single Drop ◽

Microwave Assisted ◽

Assisted Extraction ◽

Single Drop Microextraction ◽

Limit Of Quantitation

Objective: The objective of this research was to improve the method of extracting pesticide residue from vegetable by using microwave-assisted extraction, headspace single drop microextraction (MAE-HS-SDME) and analysed by gas chromatograph-mass spectrometer (GC-MS).Methods: Microwave-assisted extraction (MAE) coupled with headspace single drop microextraction (HS-SDME) was used for extraction of chlorpyrifos insecticide residues in lettuce. The optimum conditions for obtaining efficient extraction have been tried; kind and quantity of solvent, extraction time, microwave irradiation power, pH value and the amount of salt added in the sample followed the method of Plackett–Burman. Optimum conditions for efficient analysis were evaluated by using a central composite design (CCD) combined with a response surface methodology (RSM).Results: The optimum extract conditions from CCD for the MAE-HS-SDME method were: using 1.5 µl of n-butyl acetate, 460 W of the microwave power, 4.5 min of the extraction time, no pH adjustment and no salt addition. Verification of the optimized experimental conditions showed that validation of the determination coefficient ranges from the concentrations of 0.01 to 0.10 mg/l, equal to 0.999; the percentages of variation coefficient were ranges from 4.6 to 7.7%; the recoveries ranges from 70.0 to 79.5%; limit of detection was 0.003 mg/kg and limit of quantitation was 0.01 mg/kg.Conclusion: In this study, the MAE-HS-SDME method coupled with GC-MS was found most suitable for chlorpyrifos analysis. The results obtained from analyzing chlorpyrifos in lettuce samples indicated that this method is rapid, simple, sensitive, reducible cost and safety for the analyst and the environment.

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Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyus Rehd.)

Antioxidants ◽

10.3390/antiox9080678 ◽

2020 ◽

Vol 9 (8) ◽

pp. 678

Author(s):

Ao Shang ◽

Min Luo ◽

Ren-You Gan ◽

Xiao-Yu Xu ◽

Yu Xia ◽

...

Keyword(s):

Antioxidant Capacity ◽

Microwave Power ◽

Ethanol Concentration ◽

Extraction Time ◽

Extraction Temperature ◽

Microwave Assisted Extraction ◽

Optimal Conditions ◽

Microwave Assisted ◽

Assisted Extraction ◽

Sample Ratio

In this study, the effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyus Rehd.) were studied and the antioxidants in the extract were identified. The influences of ethanol concentration, solvent-to-sample ratio, microwave power, extraction temperature and extraction time on Trolox equivalent antioxidant capacity (TEAC) value, ferric reducing antioxidant power (FRAP) value and total phenolic content (TPC) were investigated by single-factor experiments. The response surface methodology (RSM) was used to study the interaction of three parameters which had significant influences on antioxidant capacity including ethanol concentration, solvent-to-sample ratio and extraction time. The optimal conditions for the extraction of antioxidants from sweet tea were found as follows—ethanol concentration of 58.43% (v/v), solvent-to-sample ratio of 35.39:1 mL/g, extraction time of 25.26 min, extraction temperature of 50 ℃ and microwave power of 600 W. The FRAP, TEAC and TPC values of the extract under the optimal conditions were 381.29 ± 4.42 μM Fe(II)/g dry weight (DW), 613.11 ± 9.32 μM Trolox/g DW and 135.94 ± 0.52 mg gallic acid equivalent (GAE)/g DW, respectively. In addition, the major antioxidant components in the extract were detected by high-performance liquid chromatography with diode array detection (HPLC-DAD), including phlorizin, phloretin and trilobatin. The crude extract could be used as food additives or developed into functional food for the prevention and management of oxidative stress-related diseases.

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Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity

Antioxidants ◽

10.3390/antiox8050129 ◽

2019 ◽

Vol 8 (5) ◽

pp. 129 ◽

Cited By ~ 11

Author(s):

Bao Le ◽

Kirill S. Golokhvast ◽

Seung Hwan Yang ◽

Sangmi Sun

Keyword(s):

Antioxidant Activity ◽

Superoxide Dismutase ◽

Raw Material ◽

Ulva Pertusa ◽

Extraction Time ◽

Oxidative Enzymes ◽

Microwave Assisted Extraction ◽

Extraction Technology ◽

Microwave Assisted ◽

Assisted Extraction

The use of green marine seaweed Ulva spp. as foods, feed supplements, and functional ingredients has gained increasing interest. Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides. The antioxidant activity of ulvan was also evaluated. The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated. The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities. A response surface methodology based on a Box–Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381. Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro. Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT). The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.

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Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery (Apium graveolens L.) leaves by response surface methodology

Czech Journal of Food Sciences ◽

10.17221/266/2015-cjfs ◽

2016 ◽

Vol 34 (No. 4) ◽

pp. 341-349 ◽

Cited By ~ 5

Author(s):

Q. He ◽

Y. Li ◽

P. Zhang ◽

A. Zhang ◽

H. Wu

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Ethanol Concentration ◽

Apium Graveolens ◽

Solvent Ratio ◽

Microwave Assisted Extraction ◽

Inhibition Rate ◽

Microwave Assisted ◽

Assisted Extraction

The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.

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PENGARUH KONSENTRASI ETANOL TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruitz & Pav) MENGGUNAKAN METODE MICROWAVE ASSISTED EXTRACTION (MAE)

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2019.v08.i03.p06 ◽

2019 ◽

Vol 8 (3) ◽

pp. 278

Author(s):

I Gede Tirta Yasa ◽

Nengah Kencana Putra ◽

Anak Agung Istri Sri Wiadnyani

Keyword(s):

Antioxidant Activity ◽

Ethanol Concentration ◽

Total Phenol ◽

Total Flavonoids ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Randomized Design ◽

Assisted Extraction ◽

Completely Randomized Design ◽

Test Treatment

This study was conducted to determine the effect of ethanol concentration on antioxidant activity and to obtain the highest antioxidant activity in red betel leaves extract using the MAE method. The experimental design used in this study was a completely randomized design (CRD) with an ethanol concentration treatment consisting of five levels, namely 50%, 60%, 70%, 80%, and 90%. All treatments were repeated three times to obtain 15 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan test. Treatment of ethanol concentration had a very significant effect (P <0.01) on yield, total phenol, total flavonoids, and antioxidant activity. The results showed that 90% ethanol concentration was the best treatment which produced a yield of 25.17%, total phenol 106.00 mg GAE / g extract, total flavonoids 46.98 mg QE / g and antioxidant activity based on IC50 at 81.61 ppm. Keywords : red betel leaves, ethanol, MAE, antioxidant activity.

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Study on Technology for Obtaining Quercetin from Linifolius Conyza by Microwave-Assisted Extraction

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1817 ◽

2012 ◽

Vol 550-553 ◽

pp. 1817-1820

Author(s):

Wei Guang Zhang ◽

Ji Hong Liang ◽

You Jie Cai

Keyword(s):

Absorption Spectroscopy ◽

Ethanol Concentration ◽

Microwave Assisted Extraction ◽

Single Factor ◽

Solvent Concentration ◽

Microwave Assisted ◽

Assisted Extraction ◽

Microwave Time ◽

Microwave Assisted Method

Determination of quercetin from Linifolius Conyza by microwave-assisted extraction and absorption spectroscopy was reported. The effects of solvent concentration, microwave time, ratio of solution to solid and microwave-assisted extraction pressure were evaluated by single factor experiments. The extraction conditions of quercetin from Linifolius Conyza by microwave -assisted method were optimized: ethanol concentration is 70 %( V/ V); microwave time is 5 minutes ; ratio of solution to solid is 45:1 and microwave-assisted extraction pressure is 500kPa . microwave - assisted extraction and absorption spectroscopy was simple and accurate with good reproducibility.

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