scholarly journals Optimization of Microwave-Assisted Extraction of Polysaccharides from Ulva pertusa and Evaluation of Their Antioxidant Activity

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 129 ◽  
Author(s):  
Bao Le ◽  
Kirill S. Golokhvast ◽  
Seung Hwan Yang ◽  
Sangmi Sun
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Raw Material ◽  
Ulva Pertusa ◽  
Extraction Time ◽  
Oxidative Enzymes ◽  
Microwave Assisted Extraction ◽  
Extraction Technology ◽  
Microwave Assisted ◽  
Assisted Extraction

The use of green marine seaweed Ulva spp. as foods, feed supplements, and functional ingredients has gained increasing interest. Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides. The antioxidant activity of ulvan was also evaluated. The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated. The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities. A response surface methodology based on a Box–Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381. Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro. Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT). The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.

scholarly journals Simultaneous Optimization of Microwave-Assisted Extraction of Phenolic Compounds and Antioxidant Activity of Avocado (Persea americana Mill.) Seeds Using Response Surface Methodology

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Alexander Weremfo ◽  
Felix Adulley ◽  
Martin Adarkwah-Yiadom
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

This study was designed to optimize three microwave-assisted extraction (MAE) parameters (ethanol concentration, microwave power, and extraction time) of total phenolics, total flavonoids, and antioxidant activity of avocado seeds using response surface methodology (RSM). The predicted quadratic models were highly significant (p<0.001) for the responses studied. The extraction of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity was significantly (p<0.05) influenced by both microwave power and extraction time. The optimal conditions for simultaneous extraction of phenolic compounds and antioxidant activity were ethanol concentration of 58.3% (v/v), microwave power of 400 W, and extraction time of 4.8 min. Under these conditions, the experimental results agreed with the predicted values. MAE revealed clear advantages over the conventional solvent extraction (CSE) in terms of high extraction efficiency and antioxidant activity within the shortest extraction time. Furthermore, high-performance liquid chromatography (HPLC) analysis of optimized extract revealed the presence of 10 phenolic compounds, with rutin, catechin, and syringic acid being the dominant compounds. Consequently, this optimized MAE method has demonstrated a potential application for efficient extraction of polyphenolic antioxidants from avocado seeds in the nutraceutical industries.

Optimization of Microwave-Assisted Extraction of Polysaccharides from Panax japlcus Var

2013 ◽  
Vol 774-776 ◽  
pp. 919-922
Author(s):  
Xiao Song ◽  
Peng Zhao
Keyword(s):  
Microwave Power ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Raw Material ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Optimum Extraction

Polysaccharide fromPanax japlcus var(PJVPs) was extracted with microwave-assisted (MAE). The effects of operating conditions, such as extraction time, ratio of water to raw material , and microwave power, on the extraction yield of PJVPs were studied through a Box-Behnken design (BBD). The optimum extraction conditions were as follows: extraction time 9.14 min, power 728.5 W and ratio of water to raw material 27.57ml/g. The yield of polysaccharide was 14.02 % based on the above mentioned conditions.

scholarly journals PENGARUH WAKTU EKSTRAKSI DAN KONSENTRASI ETANOL MENGGUNAKAN METODE MICROWAVE ASSISTED EXTRACTION (MAE) TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK RAMBUT JAGUNG (Zea mays L.)

2019 ◽  
Vol 8 (1) ◽  
pp. 94
Author(s):  
Yessica Kristanti ◽  
I Wayan Rai Widarta ◽  
I Dewa Gede Mayun Permana
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Corn Silk ◽  
Assisted Extraction ◽  
Ic50 Value ◽  
Two Factors ◽  
Determine Effect

This research was conducted to determine effect of the extraction time and ethanol concentration on antioxidant activity of corn silk extract and to obtain extraction time and ethanol concentration using Microwave Assisted Extraction (MAE) that can produce extract with the highest antioxidant activity. The experimental design used in this research was a completely randomize design, which consisted of two factors. The fisrt factor was the concentration of ethanol which consisted of 5 level that namely ethanol 30%, 45%, 60%, 75%, and 90%. The second factor was the time of extraction which consisted of 3 level namely 8, 13 and 18 minutes. Data were analyzed with analysis of variance that followed by Duncan test. The result showed that the best treatment of corn silk extract is extraction by ethanol 90% for 18 minutes using MAE which the highest resulted rendemen was 24.54%, total phenol was 159.65 mg GAE/g extract, total flavonoid was 149 mgQE/g extract, antioxidant activity was 70.18%, and the IC50 value 64.806 ppm.

scholarly journals Ekstraksi Senyawa Fenolik Daun Kenikir (Cosmos caudatus) menggunakan Microwave Assisted Extraction (MAE)

2018 ◽  
Vol 11 (1) ◽  
pp. 59-70
Author(s):  
Angky Wahyu Putranto ◽  
Shinta Rosalia Dewi ◽  
Ni'matul Izza ◽  
Dian Rahmat Yuneri ◽  
Maria Yeniaska S. Dachi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Microwave Assisted ◽  
Cosmos Caudatus ◽  
Assisted Extraction

Abstrak. Kenikir (Cosmos caudatus) adalah tanaman yang sering dimanfaatkan daunnya untuk bahan pangan maupun juga sebagai obat-obatan karena memiliki kandungan senyawa fenolik yang cukup tinggi. Ekstraksi senyawa fenol menggunakan metode konvensional masih memerlukan waktu yang cukup lama dan jumlah pelarut yang banyak. Sehingga perlu dicari alternatif metode ekstraksi yang memiliki waktu ekstraksi dan kebutuhan pelarut yang relatif rendah seperti menggunakan Microwave Assisted Extraction (MAE). Tujuan dari penelitian ini adalah melakukan ekstraksi senyawa fenolik dari daun kenikir dengan metode MAE terhadap total fenol dan aktivitas antioksidan. Penelitian ini menggunakan 2 faktor perlakuan yaitu rasio pelarut terhadap kenikir segar yaitu 1:4, 1:6, dan 1:8 (b/v) dan waktu ekstraksi 2, 3 dan 4 menit. Penelitian ini menjelaskan bahwa nilai total fenol tertinggi (17,162 ± 0,11 mg GAE/g berat segar) diperoleh pada perlakuan rasio pelarut 1:8 (b/v), sedangkan aktivitas antioksidan tertinggi (4,203 ± 0,26 mg/ml) pada perlakuan rasio pelarut 1:4 (b/v). Waktu perlakuan 3 menit menunjukkan hasil yang optimal untuk nilai total fenol dan aktivitas antioksidan. Hasil tersebut juga menunjukkan nilai yang lebih baik jika dibandingkan dengan metode konvensional baik dengan maserasi biasa dan maserasi dengan waterbath. Extraction of Phenolic Compounds from Cosmos caudatus using Microwave Assisted Extraction (MAE) Abstract. Cosmos caudatus, a plant commonly used its leaves for food and also as a medicine since it contains high phenolic compounds. The extraction of phenolic compounds using conventional methods still require more extraction time and need much more of solvent. Therefore, it was necessary to find an alternative extraction method that has a rapid extraction time and a few solvent need such as using Microwave Assisted Extraction (MAE). The aim of this study is to extract of phenolic compounds from Cosmos caudatus with MAE method of total phenol and antioxidant activity. This study using 2 treatment factors, the sampel-solvent ratio of 1:4, 1:6, and 1:8 (w/v) and the extraction time of 2, 3 and 4 minutes. This study explained that the highest total phenolic compounds (17.162 ± 0.11 mg GAE/g fw) and antioxidant activity (4.203 ± 0.26 mg/ml) were obtained at 1:8 and 1:4 (w/v) of sampel-solvent ratio, respectively. The extraction time of 3 minutes was the optimal condition for total phenolic compounds and antioxidant activity. These results also showed the highest parameter value when compared with both conventional methods: maceration and maceration with waterbath. 

Microwave Assisted Extraction (MAE) Process of Tannin from Mangrove Propagules Waste as Natural Dye for Coloring Batik tulis

2019 ◽  
Vol 805 ◽  
pp. 128-133 ◽  
Author(s):  
Prima A. Handayani ◽  
Achmad Chafidz ◽  
Nur Salsabilah Ramadani ◽  
Dewi Kartika
Keyword(s):  
Natural Dye ◽  
Raw Material ◽  
Extraction Time ◽  
Hydroxyl Groups ◽  
Solvent Ratio ◽  
Distilled Water ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ft Ir

Mangrove propagule waste is one of the biomass wastes that decays fast, hence it can pollute the environment. Mangrove propagule contains 30.43% tannin, and thus it has high potential to be used as a natural dye for coloringBatik Tulis. Unfortunately, this raw material has not been utilized optimally. The objective of this study is to extract tannin substances from mangrove propagules by using Microwave Assisted Extraction (MAE) method. The effects of solvent types (i.e. ethanol and distilled water), material-solvent ratio, extraction time and temperature on the yield of tannin obtained have also been investigated. The extraction results were analyzed by FT-IR to determine the presence of tannin contained in the dyes. The extraction results showed that the optimum yield of etanol solvent was 27.5%, which occured at material-solvent ratio of 0.04 g/ml, extraction time of 30 minutes, and temperature of 60°C. Whereas, the distilled water produced 22.7% yield of tannin, which also occured at material and solvent ratio 0.04 g/ml, extraction time of 30 minutes, and temperature of 80°C. Additionally, FT-IR analysis of tannin extract from mangrove propagules with etanol and distilled water showed the presence of hydroxyl groups (O-H), groups (C-H), C = C groups, and C-O groups.

Optimization of Microwave-Assisted Extraction of Polysaccharides from Acanthopanax Obouatus Hoo

2012 ◽  
Vol 549 ◽  
pp. 504-507
Author(s):  
Xiao Rong Li ◽  
Peng Zhao
Keyword(s):  
Microwave Power ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Raw Material ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Optimum Extraction

Polysaccharide from Acanthopanax obouatus Hoo(AHPs) was extracted with microwave-assisted(MAE). The effects of operating conditions, such as extraction time, ratio of water to raw material , and microwave power, on the extraction yield of AHPs were studied through a Box-Behnken design (BBD). The optimum extraction conditions were as follows: extraction time 13.5min, power 796 W and ratio of water to raw material 16.5ml/g. The yield of polysaccharide was 5.18% based on the above mentioned conditions.

scholarly journals Sifat Fisikokimia Zat Pewarna dari Bunga Eceng Gondok (Eichhornia crassipes) yang Diekstrak dengan Metode Microwave Asissted Extraction (MAE)

2019 ◽  
Vol 8 (1) ◽  
pp. 94-106
Author(s):  
Merynda Indriyani Syafutri ◽  
Filli Pratama ◽  
Gading Putra Yanda
Keyword(s):  
Antioxidant Activity ◽  
Microwave Power ◽  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Color Intensity ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Anthocyanin Stability

Syafutri et al, 2019. Physicochemical Properties of Colorant from Water Hyacinth Flower (Eichhornia crassipes) Extracted by Microwave Assisted Extraction (MAE) Method. JLSO 8(1):94-106.The objective of this research was to determine the effect of oven microwave power and extraction time on color intensity, anthocyanin stability, and antioxidant activity of colorant from water hyacinth flower (Eichhornia crassipes) extracted by microwave assisted extraction (MAE) method. The research was conducted at Agricultural Product Chemical Laboratory, Agricultural Technology Department, Faculty of Agriculture, Sriwijaya University. The experiment used a Factorial Completely Randomized Design with two factors and each treatment was repeated three times.  The first factor was oven microwave power (100 watts and 300 watts) and the second factor was extraction time in oven microwave (1 minute, 2 minutes, and 3 minutes). The parameters were color intensity, anthocyanin stability, and antioxidant activity. The results showed that the oven microwave power and extraction time had significant effects on color intensity and antioxidant activity. The increasing of oven microwave power and extraction time increased a* value and antioxidant activity, and decreased b* value, significantly. The result also showed that the anthocyanin compounds was not stable with heating up to 105°C.

scholarly journals Kandungan Flavonoid dan Aktivitas Antioksidan Ekstrak Pleurotus ostreatus

2018 ◽  
Vol 11 (1) ◽  
pp. 1-10
Author(s):  
Shinta Rosalia Dewi ◽  
Bambang Dwi Argo ◽  
Naily Ulya
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Colorimetric Method ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Flavonoid Content ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Dpph Method

Abstrak. Flavonoid merupakan senyawa fenolik yang banyak diisolasi dari tanaman karena manfaatnya sebagai antioksidan, anti mikroba, dan antikanker. Sebagai antioksidan, flavonoid dapat menangkap radikal bebas yang dapat merusak sel tubuh. Oleh karena itu, kandungan total flavonoid (TFC) dan aktivitas antioksidan (IC50) ekstrak jamur Pleurotus ostreatus penting untuk diteliti. Flavonoid diekstrak menggunakan metode Microwave-Assisted Extraction (MAE) dengan variasi waktu ekstraksi (2, 3, dan 4 menit) dan variasi rasio antara jamur dan pelarut (1:30, 1:35, dan 1:40). Kandungan flavonoid dianalisis dengan metode kolorimetri menggunakan alumunium klorida, sedangkan aktivitas antioksidan ditentukan dengan metode DPPH. Hasil penelitian menunjukkan bahwa semakin lama waktu ekstraksi, semakin tinggi TFC dan aktivitas antioksidan. Kandungan flavonoid tertinggi diperoleh pada perlakuan waktu ekstraksi 4 menit dan rasio jamur:pelarut 1:30, yaitu sebesar 1,53 mg QE/ g dw dengan nilai IC50 sebesar 14,66 mg/ml. Flavonoid Content and Antioxidant Activity of Pleurotus ostreatus Extract Abstract. Flavonoids-phenolic substances- are isolated from plant due to their benefits as an antioxidant, an antimicrobial, and an anticancer. As an antioxidant, flavonoids can scavenge free radicals that damage body cells. In this study, the total flavonoid content (TFC) and its antioxidant activity (IC50) of Pleurotus ostreatus -an oyster mushroom- extract were investigated. The flavonoid was extracted by using Microwave-assisted Extraction (MAE) at different of extraction time (2, 3, and 4 minutes) and ratio of P.ostreatus and solvent (1:30, 1:35 and 1:40). The flavonoid contents were determined by alumunium chloride colorimetric method whereas the antioxidant activity was determined by DPPH method. The results revealed that the higher extraction time, the higher TFC and antioxidant activity, where the highest TFC was obtained at 4 minutes extraction with ratio of P.ostreatus and solvent of 1:30. The highest TFC of P.ostreatus extract was 1.53 mg QE/ g dw with antioxidant activity (IC50) of 14.66 mg/ml.

scholarly journals Optimization Of Microwave-Assisted Extraction Of Flavonoids From Binahong (Anredera cordifolia) Leaves Using Respon Surface Methodology

2020 ◽  
Vol 7 (3) ◽  
pp. 10-19
Author(s):  
Zaldy Rusli ◽  
Bina Lohita Sari ◽  
Novi Fajar Utami ◽  
S Sabila
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Extraction Process ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Optimum Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction

Binahong (Anredera scandens (L.) Moq.) leaves are one of the plants that empirically can be used for wound healing and contain flavonoids which have antibacterial and antioxidant activities. Determination of the optimum conditions of the Binahong leaf extraction process needs to be done to ensure the extraction quality of the Binahong leaves associated with the given activity. Microwave Assisted Extraction (MAE) is an extraction that utilizes microwave radiation to heat the solvent quickly and efficiently. This study aims to determine the most effective binahong leaf extraction conditions that produce optimal levels of flavonoids and antioxidant activity. The extraction process was carried out using the Microwave assisted extraction (MAE) method. Determination of optimum conditions is done based on Response Surface Method (RSM) with variable ethanol concentration (70%, 80% and 90%), extraction time (4, 12 and 20 minutes) and power (450, 600, and 800 watts) using Box- Behnken Design (BBD) with Design Expert 7.0 software. The extract quality parameters measured were total flavonoid levels and antioxidant activity. The results showed the optimum conditions with the BBD method were obtained at an ethanol concentration of 81.49%, extraction time of 13.84 minutes, and power of 626.19 watts with flavonoid levels of 3.8561% and antioxidant activity (IC50) of 95.51834 ppm with active categories.

Sign in / Sign up

Export Citation Format

Share Document