scholarly journals Diagnostic validation of two SARS-CoV-2 immunochromatographic tests in Slovenian and Croatian hospitals

2021 ◽  
Vol 62 (5) ◽  
pp. 513-517
Author(s):  
Martina Ifko ◽  
Željka Tkalčić Švabek ◽  
Iva Friščić ◽  
Mirjana Mariana Kardum Paro ◽  
Ingrid Prkačin ◽  
...  
Keyword(s):  
Diagnostic Validation

Diagnostic Validation Study. Relationship Between Optical Spectroscopy and Ankle Brachial Index Tests for Peripheral Artery Disease.

2021 ◽  
Author(s):  
Concepción Rodríguez Nieves Aleicel ◽  
Riera del Moral Luis Felipe ◽  
Gutiérrez Nistal Marta ◽  
Zafra Angulo Juan ◽  
Fernández Heredero Álvaro
Keyword(s):  
Optical Spectroscopy ◽  
Peripheral Artery Disease ◽  
Validation Study ◽  
Ankle Brachial Index ◽  
Peripheral Artery ◽  
Index Tests ◽  
Diagnostic Validation ◽  
Artery Disease

High-gamma modulation language mapping with stereo-EEG: A novel analytic approach and diagnostic validation

2020 ◽  
Vol 131 (12) ◽  
pp. 2851-2860 ◽  
Author(s):  
Brian Ervin ◽  
Jason Buroker ◽  
Leonid Rozhkov ◽  
Timothy Holloway ◽  
Paul S. Horn ◽  
...  
Keyword(s):  
Analytic Approach ◽  
Language Mapping ◽  
High Gamma ◽  
Diagnostic Validation

scholarly journals Diagnostic Validation of a Whole-Slide Imaging Scanner in Cytological Samples: Diagnostic Accuracy and Comparison With Light Microscopy

2019 ◽  
Vol 56 (3) ◽  
pp. 429-434 ◽  
Author(s):  
Federico Bonsembiante ◽  
Ugo Bonfanti ◽  
Francesco Cian ◽  
Laura Cavicchioli ◽  
Beatrice Zattoni ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Diagnostic Accuracy ◽  
Gold Standard ◽  
Cytological Diagnosis ◽  
Whole Slide Imaging ◽  
Glass Slides ◽  
Remote Sites ◽  
Diagnostic Validation ◽  
Sensitivity Specificity ◽  
Different Levels

Digital slides created by whole-slide imaging scanners can be evaluated by pathologists located in remote sites, but the process must be validated before this technology can be applied to routine cytological diagnosis. The aim of this study was to validate a whole-slide imaging scanner for cytological samples. Sixty cytological samples, whose diagnoses were confirmed by gold-standard examinations (histology or flow cytometry), were digitalized using a whole-slide imaging scanner. Digital slides and glass slides were examined by 3 observers with different levels of cytopathological expertise. No significant differences were noted between digital and glass slides in regard to the number of cases correctly diagnosed, or the sensitivity, specificity, or diagnostic accuracy, irrespective of the observers’ expertise. The agreements between the digital slides and the gold-standard examinations were moderate to substantial, while the agreements between the glass slides and the gold-standard examinations were substantial for all 3 observers. The intraobserver agreements between digital and glass slides were substantial to almost perfect. The interobserver agreements when evaluating digital slides were moderate between observers 1 and 2 and between observers 1 and 3 while they were substantial between observers 2 and 3. In conclusion, our study demonstrated that the digital slides produced by the whole-slide imaging scanner are adequate to diagnose cytological samples and are similar among clinical pathologists with differing levels of expertise.

Trauma-Induced Dissociative Amnesia in World War I Combat Soldiers. II. Treatment Dimensions

1999 ◽  
Vol 33 (3) ◽  
pp. 392-398 ◽  
Author(s):  
Paul Brown ◽  
Onno van der Hart ◽  
Mariétte Graafland
Keyword(s):  
Sexual Abuse ◽  
World War I ◽  
Treatment Approach ◽  
Cognitive Integration ◽  
World War ◽  
Traumatic Memories ◽  
Posttraumatic Amnesia ◽  
Dissociative Amnesia ◽  
Civilian Trauma ◽  
Diagnostic Validation

Objective: This is the second part of a study of posttraumatic amnesia in World War I (WW I) soldiers. It moves beyond diagnostic validation of posttraumatic amnesia (PTA), to examine treatment findings, and relates these to contemporary treatment of dissociative amnesia, including treatment of victims of civilian trauma (e.g. childhood sexual abuse). Method: Key WW I studies are surveyed which focus on the treatment of PTA and traumatic memories. The dissociation-integration and repression-abreaction models are contrasted. Results: Descriptive evidence is cited in support of preferring Myers' and McDougalls' dissociation-integration treatment approach over Brown's repression-abreaction model. Conclusion: Therapeutic findings in this paper complement diagnostic data from the first report. Although effective treatment includes elements of both the dissociative-integrative and abreactive treatment approaches, cognitive integration of dissociated traumatic memories and personality functions is primary, while emotional release is secondary.

scholarly journals Analytical and diagnostic validation of a flow cytometric strategy to quantify blood and marrow infiltration in dogs with large B-cell lymphoma

2016 ◽  
Vol 90 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Fulvio Riondato ◽  
Barbara Miniscalco ◽  
Alessia Poggi ◽  
Arianna Aricò ◽  
Luca Aresu ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Flow Cytometric ◽  
Large B Cell Lymphoma ◽  
Diagnostic Validation ◽  
Large B Cell

Differential Diagnostic Validation: Acute and Chronic Pain

1995 ◽  
Vol 6 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Jolene M. Simon ◽  
Martha A. Baumann ◽  
Lucyanne Nolan
Keyword(s):  
Chronic Pain ◽  
Diagnostic Validation

47 Diagnostic validation of urinary Tyr-phosphorylated proteins as a new marker for bladder cancer

2014 ◽  
Vol 13 (1) ◽  
pp. e47
Author(s):  
P. Destefanis ◽  
A. Battaglia ◽  
M. Allasia ◽  
S. Chiesa ◽  
E. Garzino ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Phosphorylated Proteins ◽  
Diagnostic Validation

scholarly journals In silico design and validation of commercial kit GPS™ CoVID-19 dtec-RT-qPCR Test under criteria of UNE/EN ISO 17025:2005 and ISO/IEC 15189:2012

2020 ◽  
Author(s):  
Antonio Martínez-Murcia ◽  
Gema Bru ◽  
Aaron Navarro ◽  
Patricia Ros-Tárraga ◽  
Adrián García-Sirera ◽  
...  
Keyword(s):  
In Silico ◽  
Virus Disease ◽  
Quantitative Phase Analysis ◽  
Iso 17025 ◽  
The Public ◽  
Transfer Of Technology ◽  
Validation Parameters ◽  
Diagnostic Validation ◽  
Diagnostic Sensitivity And Specificity ◽  
Carlos Iii

ABSTRACTBackgroundThe Corona Virus Disease 2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), has become a serious infectious disease affecting human health worldwide and rapidly declared a pandemic by WHO. Early, several RT-qPCR were designed by using only the first SARS-CoV-2 genome sequence.ObjectivesA few days later, when additional SARS-CoV-2 genome were retrieved, the kit GPS™ CoVID-19 dtec-RT-qPCR Test was designed to provide a highly specific detection method and commercially available worldwide. The kit was validated following criteria recommended by the UNE/EN ISO 17025:2005 and ISO/IEC 15189:2012.MethodsThe present study approached the in silico specificity of the GPS™ CoVID-19 dtec-RT-qPCR Test and RT-qPCR designs currently published. The empirical validation parameters specificity (inclusivity/exclusivity), quantitative phase analysis (10-106 copies), reliability (repeatability/reproducibility) and sensitivity (detection/quantification limits) were evaluated for a minimum of 10-15 assays. Diagnostic validation was achieved by two independent reference laboratories, the Instituto de Salud Carlos III (ISCIII), (Madrid, Spain) and the Public Health England (PHE; Colindale, London, UK).ResultsThe GPS™ RT-qPCR primers and probe showed the highest number of mismatches with the closet related non-SARS-CoV-2 coronavirus, including some indels. The kits passed all parameters of validation with strict acceptance criteria. Results from reference laboratories 100% correlated with these obtained by suing reference methods and received an evaluation with 100% of diagnostic sensitivity and specificity.ConclusionsThe GPS™ CoVID-19 dtec-RT-qPCR Test, available with full analytical and diagnostic validation, represents a case of efficient transfer of technology being successfully used since the pandemic was declared. The analysis suggested the GPS™ CoVID-19 dtec-RT-qPCR Test is the more exclusive by far.

Targeted deep sequencing as a diagnostic and research method for detecting EGFR and ALK pathway driver genes in FFPE tissue of lung and colorectal carcinomas.

2012 ◽  
Vol 30 (30_suppl) ◽  
pp. 39-39
Author(s):  
Sakari Knuutila ◽  
Katja Merkkiniemi ◽  
Mikko Rönty ◽  
Aino Wirtanen ◽  
Satu Maria Remes ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Deep Sequencing ◽  
Lung Carcinoma ◽  
Copy Number ◽  
Mutation Screening ◽  
Gene Fusions ◽  
Copy Number Alterations ◽  
Hospital District ◽  
Targeted Deep Sequencing ◽  
Diagnostic Validation

39 Background: Molecular targeted tyrosine kinase inhibitor (TKI) treatments have made it crucial to perform diagnostic tests of multiple molecular targets. In lung carcinoma there are close to ten clinically relevant gene mutations, copy number alterations and/or gene fusions, such as ALK, EGFR, ERBB2, KRAS, BRAF, MET, PTEN, PI3KCA, ROS1 and RET. Presently, several different tests are utilized, requiring a high amount of tumor material and long turnaround time. Next generation sequencing or targeted deep sequencing (TDS) has opened a new era for rapid genome-wide analyses of mutations, copy number alterations and gene fusions. Our aimwas to 1) prove feasibility for applying TDS to FFPE samples, 2) compare mutations detected by prevalent methods & TDS, and 3) mine novel clinically and biologically relevant genes in lung and colorectal carcinoma. Methods: For TDS, we selected 192 lung carcinoma and colorectal carcinoma related genes and microRNA genes, focusing on the EGFR and ALK pathways. In total, 98 FFPE specimens were studied. Agilent SureSelect system and Illumina sequencing was adopted for the analysis. For diagnostic validation the following genes were selected: EGFR, KRAS, BRAF, PTEN, PI3K, RET and ALK. TDS results were confirmed by PCR, FISH and IHC. Results: We focused on the genes selected for diagnostic validation. Successful results were obtained from all specimens. The results from TDS correlated significantly with those obtained from PCR, FISH, and IHC. Importantly, TDS revealed novel mutations not detected by targeted PCR. Conclusions: An enormous advantage of TDS is that multiple mutation screening can be achieved in one analysis (saving time and material), and most importantly, provides enormous amounts of novel information, for example understanding mechanisms for drug resistance. This study was supported by Finnish Academy, Sigrid Jusélius Foundation, Finnish Cancer Organizations, the special governmental subsidy research funds appropriated to the Helsinki and Uusimaa Hospital District (HUS EVO), Pfizer Oy, AstraZeneca AS, Lab21 Ltd, Abbott Molecular Inc.

Sign in / Sign up

Export Citation Format

Share Document