In Vitro and In Vivo Effect of the Composition of Fucoxanthin with Porous Aluminum-Silicon Career on Cells

Fucoxanthin natural carotenoid from seaweeds exhibits antioxidant properties. The paper analyzes the possibility of using fucoxanthin, immobilized on aluminum-silicon carrier particles, to assess the toxic effect on naïve or matured immune cells, enterocytes, Mesenchymal stem cells, and EA.Hy926 cell line. The proliferation, nitric oxide production, myeloperoxidase activity, and apoptosis of thymocytes and splenocytes of mice in vitro were studied. Proliferative capacity, nitric oxide production, and apoptosis rate of entreocytes, mesenchymal stem cells and EA.Hy926 cell line were investigated. It is shown that fucoxanthin immobilized on aluminum-silicon carrier particles increased the survival rate and proliferation of mature immunocytes (splenocytes) after 24 hours exposure and increased the survival rate of naïve immunocytes (thymocytes) when exposed 120 hours. On myeloperoxidase, the activity of immune cells is not affected by fucoxanthin immobilized on the carrier particles. Orally the administration of some samples of fucoxanthin immobilized on the carrier particles with different sizes, and dosage no have an adverse effect on jejunum enterocytes (proliferation and apoptosis). In vitro, all tested samples of fucoxanthin immobilized on the carrier particles with different sizes and dosage in common no have a toxic effect on jejunum enterocytes, human bone marrow mesenchymal stem cells, and EA.Hy926 cell line. The obtained results indicate that fucoxanthin, immobilized on particles of an aluminum-silicon carrier, does not have a toxic effect on mouse immunocytes and enterocytes, and human mesenchymal stem cells and EA.Hy92 cell line.

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The Influence of Fucoxanthin Immobilized on Porous Aluminum-Silicon Carrier Surface on the Functional Activities of Immunocytes in Mice

KnE Life Sciences ◽

10.18502/kls.v7i1.10109 ◽

2022 ◽

Author(s):

Alexander Lykov ◽

Lubov Rachkovskaya ◽

Ruslan Gevorgiz ◽

Svetlana Zheleznova ◽

Olga Poveshchenko

Keyword(s):

Nitric Oxide ◽

Survival Rate ◽

Toxic Effect ◽

Immune Cells ◽

Antioxidant Properties ◽

Myeloperoxidase Activity ◽

Porous Aluminum ◽

Cylindrotheca Closterium ◽

Aluminum Silicon

Fucoxanthin is a natural carotenoid obtained from seaweed which exhibits antioxidant properties. This research aimed to assess whether fucoxanthin, immobilized on aluminum-silicon carrier particles, has a toxic effect on immune cells. The viability, proliferation, nitric oxide production and myeloperoxidase activity of thymocytes and splenocytes of mice in vitro were studied. It was shown that fucoxanthin, immobilized on aluminum-silicon carrier particles, increased the survival rate and proliferation of mature immunocytes (splenocytes) after 24 hours exposure and increased the survival rate of naïve immunocytes (thymocytes) when exposed for 120 hours. In terms of myeloperoxidase, the activity of the immune cells was not affected by fucoxanthin immobilized on the carrier particles. The obtained results indicated that fucoxanthin, immobilized on particles of an aluminum-silicon carrier, did not have a toxic effect on mouse immunocytes. Keywords: Cylindrotheca closterium, fucoxanthin, γ-aluminum oxide, polydimethylsiloxane, thymocytes, splenocytes, viability, proliferation, nitric oxide, myeloperoxidase activity

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Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells

Acta Histochemica ◽

10.1016/j.acthis.2008.02.005 ◽

2009 ◽

Vol 111 (1) ◽

pp. 15-24 ◽

Cited By ~ 27

Author(s):

Monia Orciani ◽

Oriana Trubiani ◽

Arianna Vignini ◽

Monica Mattioli-Belmonte ◽

R. Di Primio ◽

...

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Periodontal Ligament ◽

Nitric Oxide Production ◽

Oxide Production

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Comparison of TGF-β and Nitric Oxide Production by Adipose-Derived Mesenchymal Stem Cells Between Healthy Pregnant and Preeclamptic Women

Immunoregulation ◽

10.32598/immunoregulation.1.1.11 ◽

2018 ◽

pp. 11-20

Author(s):

Ensie Sadat Mirsharif ◽

Sakine Moaiedmohseni ◽

Seyyed Mahmoud Hashemi ◽

Eisa Salehi ◽

Tooba Ghazanfari

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nitric Oxide Production ◽

Oxide Production

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Homoeopathic Viscum album (10-3) is More Cytotoxic to in vitro Culture of Human Breast Cancer Cell Line than to Human Mesenchymal Stem Cells

International Journal of High Dilution Research - ISSN 1982-6206 ◽

10.51910/ijhdr.v19i4.1036 ◽

2021 ◽

Vol 19 (4) ◽

pp. 25-34

Author(s):

Ana Catarina Viana Valle ◽

Lana Ribeiro Aguiar ◽

Hilana dos Santos Sena Brunel ◽

Patricia Furtado Malard ◽

Carla Lujan Pereira Villarroel ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cell Line ◽

Culture Medium ◽

Cancer Cell Line ◽

Viscum Album ◽

Human Breast Cancer Cell ◽

Human Breast ◽

Mcf 7

BACKGROUND Adenocarcinomas can be of several types, and MCF-7 is an adenocarcinoma of human breast cell line useful as preclinical model to screen therapeutic agents such as ultra-diluted Viscum album, an European plant whose extract is commonly used in cancer therapy. AIMS MCF-7 and mesenchymal stem cells were used to evaluate the in vitro cytotoxicity of homoeopathic Viscum album 1x10-3 (VAD3). METHODS cells were cultured for 24 hours in controlled environment (37.5oC and 5% CO2) in 96-well plates. After this time, VAD3 was added to the culture medium in concentrations varying from 10 to 100 ?L/mL for MTT assay (evaluation of viability of cells). A control group was maintained with culture medium only. After 48 hours, the procedures of analysis of cells viability were performed. RESULTS MTT assay showed that the concentrations of 42 ?L/mL and 62 ?L/mL were able to reduce cell viability to 50% in MCF-7 and mesenchymal stem cells, respectively, which means that half of the cells cultured were dead after 48 hours in contact with VAD3. CONCLUSION Viscum album presented higher cytotoxic action on human breast cancer cell line culture than on mesenchymal stem cells. This medicine is extensively used against cancer, and the use of the homoeopathic form of it brings new possibilities as no or fewer adverse effects would be present.

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A novel rat fibrosarcoma cell line from transformed bone marrow-derived mesenchymal stem cells with maintained in vitro and in vivo stemness properties

Experimental Cell Research ◽

10.1016/j.yexcr.2017.02.005 ◽

2017 ◽

Vol 352 (2) ◽

pp. 218-224 ◽

Cited By ~ 4

Author(s):

Meng-Yu Wang ◽

Janne Nestvold ◽

Øystein Rekdal ◽

Gunnar Kvalheim ◽

Øystein Fodstad

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Cell Line ◽

Fibrosarcoma Cell

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MicroRNA-Enriched Exosomes from Different Sources of Mesenchymal Stem Cells Can Differentially Modulate Functions of Immune Cells and Neurogenesis

Biomedicines ◽

10.3390/biomedicines10010069 ◽

2021 ◽

Vol 10 (1) ◽

pp. 69

Author(s):

Naina Soni ◽

Suchi Gupta ◽

Surender Rawat ◽

Vishnu Krishnakumar ◽

Sujata Mohanty ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Expression Profile ◽

Immune Cells ◽

Biologically Active ◽

Rna Seq ◽

Adult Mesenchymal Stem Cells ◽

Neutrophil Survival ◽

Different Sources

Adult Mesenchymal stem cells-derived exosomes carry several biologically active molecules that play prominent roles in controlling disease manifestations. The content of these exosomes, their functions, and effect on the immune cells may differ depending on their tissue sources. Therefore, in this study, we purified the exosomes from three different sources and, using the RNA-Seq approach, highly abundant microRNAs were identified and compared between exosomes and parental cells. The effects of exosomes on different immune cells were studied in vitro by incubating exosomes with PBMC and neutrophils and assessing their functions. The expression levels of several miRNAs varied within the different MSCs and exosomes. Additionally, the expression profile of most of the miRNAs was not similar to that of their respective sources. Exosomes isolated from different sources had different abilities to induce the process of neurogenesis and angiogenesis. Moreover, these exosomes demonstrated their varying effect on PBMC proliferation, neutrophil survival, and NET formation, highlighting their versatility and broad interaction with immune cells. The knowledge gained from this study will improve our understanding of the miRNA landscape of exosomes from hMSCs and provide a resource for further improving our understanding of exosome cargo and their interaction with immune cells.

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SOCS1 Regulates the Immune Modulatory Properties of Mesenchymal Stem Cells by Inhibiting Nitric Oxide Production

PLoS ONE ◽

10.1371/journal.pone.0097256 ◽

2014 ◽

Vol 9 (5) ◽

pp. e97256 ◽

Cited By ~ 9

Author(s):

Lei Zhang ◽

Rui-Jie Dang ◽

Hong Li ◽

Ping Li ◽

Yan-Mei Yang ◽

...

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Nitric Oxide Production ◽

Oxide Production

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Erythropoietin and Mesenchymal Stem Cells Properties

Biointerface Research in Applied Chemistry ◽

10.33263/briac105.61976207 ◽

2020 ◽

Vol 10 (5) ◽

pp. 6197-6207

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adhesion Molecules ◽

Erythropoietin Receptor ◽

Proliferative Potential ◽

Nitric Oxide Production ◽

Oxide Production ◽

Autophagy Activity

The present work reports the effect of the long-term exposure bone marrow mesenchymal stem cells from chronic ischemic heart disease patients with erythropoietin on morpho-functional properties. The mesenchymal stem cells were grown under 3rd passage in medium supplemented with 33.4 IU/mL of recormon to study expression of adhesion molecules, erythropoietin receptor, cytokine receptor to common β-chain on surface, and proliferative potential (MTT test, CFU, cell cycle), migratory ability, nitric oxide production under growth factor deprivation, oxidative stress and hyperglycemia conditions, and autophagy activity, and ultrastructure changes in cells. The results showed that the long-term exposure mesenchymal stem cells with erythropoietin significantly increased proliferation, nitric oxide production and some adhesion molecules and receptors expression on surface of those cells. In addition, the erythropoietin long-term exposure with mesenchymal stem cells increases autophagy activity. The finding of this work indicates that erythropoietin could be used to augment cell resistance to adverse microenvironment condition.

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