Potential Inhibition of Ehrlich Ascites Carcinoma by Naja Nubiae Crude Venom in Swiss Albino Mice

Cancer, the uncontrolled growth of cells, is a noteworthy cause of death globally. The present investigation attempted to study the potential efficacy of the Naja nubiae snake venom (NNSV) against Ehrlich Ascites Carcinoma (EAC) bearing mice. The LD50 was determined using twenty female mice (5 per group). The groups were given saline (Control) or venom (0.2, 0.46, 0.6 mg/kg) by intraperitoneal route. For the main experiment, fifty female mice were divided into five groups (n = 10): all groups except groups I and II received EAC cells intraperitoneal. All EAC bearing mice received intraperitoneal saline (EAC control), 0.034 mg/kg NNSV, 0.017 mg/kg NNSV, and reference drug (5- fluorouracil, 20 mg/kg body weight i.p.), respectively. In NNSV-treated mice, there were significant reductions in tumor volume, tumor cell counts, tumor cell viability, total WBC count, MDA, urea, uric acid, AST, ALT, and ALP levels. Red blood cell count, hemoglobin content, platelets, glutathione, and catalase levels increased significantly in NNSV-treated mice. The architecture of the hepatic and renal architecture in mice treated with NNSV was improved histopathologically. The effect of NNSV against Ehrlich Ascites Carcinoma was inversely dose-dependent.

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In vitro anticancer activity of Astaxanthin

World Journal of Biology Pharmacy and Health Sciences ◽

10.30574/wjbphs.2021.5.3.0110 ◽

2021 ◽

Vol 5 (3) ◽

pp. 033-037

Author(s):

Uma Nath U ◽

Ravi. R ◽

Sundara Ganapathy ◽

Lal Prasanth

Keyword(s):

Anticancer Activity ◽

Tumor Volume ◽

Hematological Parameters ◽

Ehrlich Ascites Carcinoma ◽

High Dose ◽

Ehrlich Ascites ◽

Shrimp Shell ◽

Shrimp Shell Waste ◽

Wbc Count

This study was designed to determine the in vitro anticancer potential of the Astaxanthin isolated from shrimp shell waste (ETC) against Ehrlich Ascites Carcinoma (EAC) induced cancer in swiss albino mice. The anticancer activity was assessed using in vitro cytotoxicAity, mean survival time, tumor volume and hematological studies. The reliable criteria for evaluating the potential of any anticancer agent is the prolongation of lifespan of the animal and decrease in WBC count of blood. The high dose of ETC (200 mg/kg, orally) significantly reduced the tumor growth which was demonstrated by increased lifespan of the mice and restoration of hematological parameters. ETC was also found to be cytotoxic in the in vitro parameter which shows that ETC possesses significant anticancer potential.

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Antitumor potential of Citrus limetta fruit peel in Ehrlich ascites carcinoma bearing Swiss albino mice

Alternative Medicine Studies ◽

10.4081/ams.2012.e10 ◽

2012 ◽

Vol 2 (1) ◽

pp. 10 ◽

Cited By ~ 2

Author(s):

Sriparna Kundusen ◽

Asis Bala ◽

Biswakanth Kar ◽

Sanjib Bhattacharya ◽

Upal K. Mazumder ◽

...

Keyword(s):

Antitumor Activity ◽

Tumor Cell ◽

Life Span ◽

Blood Cells ◽

Tumor Volume ◽

Ehrlich Ascites Carcinoma ◽

Viable Tumor Cell ◽

Fruit Peel ◽

Ehrlich Ascites ◽

Citrus Limetta

Citrus limetta Risso (Rutaceae), commonly known as sweet lime in English and Mousambi in India, has been traditionally used for several medicinal purposes. This study explored the relationship between Citrus limetta fruit peel and its antitumor activity against Ehrlich ascites carcinoma (EAC) bearing mice. The antitumor activity of methanol extract of peel of Citrus limetta fruits (MECL) was evaluated against EAC cell line in Swiss albino mice. Twenty-four hours after intraperitoneal inoculation of tumor EAC cells in mice, MECL was administered at 200 and 400 mg/kg body weight i.p. daily for nine consecutive days. On the 10th day, half of the mice were sacrificed for the estimation of tumor growth (tumor volume, viable and non-viable tumor cell counts), and hematologic parameters (red blood cells, white blood cells and hemoglobin). The rest were kept alive for assessment of survival parameters, i.e. median survival time and percentage increase in life span of EAC bearing mice. Intraperitoneal administration of MECL at the doses of 200 and 400 mg/kg for nine days to the carcinoma induced mice demonstrated a significant (P<0.001) decrease in tumor volume, viable tumor cell count, tumor weight and a significant (P<0.001) improvement in hematological parameters and life span as compared to the EAC control mice. The present study establishes marked and dose dependant anti-tumor effect of C. limetta fruit peel against Ehrlich ascites carcinoma bearing Swiss mice.

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Antitumor and biochemical effects of Echis coloratus crude venom on Ehrlich ascites carcinoma cells in vivo

Journal of Venomous Animals and Toxins ◽

10.1590/s0104-79302002000200008 ◽

2002 ◽

Vol 8 (2) ◽

pp. 283-296 ◽

Cited By ~ 3

Author(s):

E. A. MADY

Keyword(s):

Ehrlich Ascites Carcinoma ◽

Carcinoma Cells ◽

Crude Venom ◽

Biochemical Effects ◽

Ehrlich Ascites

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Trema orientalis (Linn.) leaves promotes anticancer activity in Ehrlich ascites carcinoma (EAC) in Swiss albino mice

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2019-0121 ◽

2019 ◽

Vol 0 (0) ◽

Author(s):

Masnoon Kabir ◽

Abdullah AL-Noman ◽

Biplab Kumar Dash ◽

Mahmudul Hasan ◽

Shahina Akhter ◽

...

Keyword(s):

Body Weight ◽

Tumor Cell ◽

Chromatin Condensation ◽

Hematological Parameters ◽

Ehrlich Ascites Carcinoma ◽

Swiss Albino Mice ◽

Ehrlich Ascites ◽

Trema Orientalis ◽

Albino Mice ◽

Eac Cells

AbstractBackgroundThe in vivo anticancer effect of the Trema orientalis leaves crude methanol extract (TLME) was screened against Ehrlich ascites carcinoma (EAC) in Swiss albino mice.Materials and methodsThe cytotoxic activity of TLME was determined in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The growth inhibitory activity and morphological alterations were determined by the hemocytometer counting of the EAC cells using trypan blue dye. The apoptotic cells were assessed by DAPI (4′,6-diamidino-2-phenylindole) staining. The hematological and biochemical parameters of experimental mice were also estimated.ResultsAfter treatment with the TLME, the viable tumor cell count, morphological changes and nuclear damages of the EAC cells were observed along with the hematological parameters of the experimental mice. The LD50 of TLME was 3120.650 mg/kg body weight, and this extract was proven to be safe at a dose of as high as 800 mg/kg body weight. The oral administration of the TLME at 400 mg/kg body weight resulted in approximately 59% tumor cell growth inhibition compared with the control mice, with considerable apoptotic features, including membrane blebbing, chromatin condensation, nuclear fragmentation and aggregation of the apoptotic bodies in DAPI staining under a fluorescence microscope. The TLME also dose-dependently restored the altered hematological parameters to approximately normal levels. The TLME exhibited bolstering cytotoxic effect against the EAC cell with the IC50 value of 29.952 ± 1.816 μg/mL.ConclusionThe TLME has potential as a natural anti-cancer product with apoptosis induction property and cytotoxicity against carcinoma cells.

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Ehrlich ascites carcinoma as model for studying the cardiac protective effects of curcumin nanoparticles against cardiac damage in female mice

Environmental Toxicology ◽

10.1002/tox.23016 ◽

2020 ◽

Vol 36 (1) ◽

pp. 105-113

Author(s):

Badriyah Alotaibi ◽

Ehab Tousson ◽

Thanaa A. El‐Masry ◽

Najla Altwaijry ◽

Asmaa Saleh

Keyword(s):

Ehrlich Ascites Carcinoma ◽

Protective Effects ◽

Cardiac Damage ◽

Female Mice ◽

Curcumin Nanoparticles ◽

Ehrlich Ascites

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A Comparative of Nutritional Impacts of Pomegranate and Beetroot on Female Mice Bearing Ehrlich Ascites Carcinoma

Archives Of Pharmacy Practice ◽

10.51847/sxv0cjyqdc ◽

2021 ◽

Vol 12 (3) ◽

pp. 48-54

Author(s):

Dalia Ismaeil Ibrahim Hemdan ◽

Nabila Yahia Mahmoud Abdulmaguid

Keyword(s):

Ehrlich Ascites Carcinoma ◽

Female Mice ◽

Ehrlich Ascites

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Hepatotoxic and hematotoxic effects of sage oil-loaded ifosfamide nanoemulsion in Ehrlich ascites carcinomabearing mice

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i6.9 ◽

2021 ◽

Vol 18 (6) ◽

pp. 1205-1211

Author(s):

Sahar M. AlMotwaa ◽

Mayson H. Alkhatib ◽

Huda M. Alkreathy

Keyword(s):

Liver Enzymes ◽

Reduced Glutathione ◽

Ehrlich Ascites Carcinoma ◽

Positive Control ◽

Negative Control ◽

Subsequent Treatment ◽

Group I ◽

Ehrlich Ascites ◽

Wbc Count ◽

Group 1

Purpose: To investigate the hepatotoxic and hematotoxic effects of sage oil-loaded ifosfamide (IFO) nanoemulsion (NE) in Ehrlich ascites carcinoma (EAC)-bearing mice. Methods: Ifosfamide (IFO) was loaded into a NE containing sage oil, and its hepatotoxic and hematotoxic effects were assessed in EAC-bearing mice. Female Swiss albino mice (n = 50) weighing 25 - 30 g (mean weight = 27.5 ± 2.50 g) were randomly assigned to five groups of ten mice each. With the exception of group 1, the mice were inoculated intraperitoneally (i.p.) with 2.5 × 106 EAC/mouse for 48 h. Group I served as negative control, C (-); group II served as positive control, C (+); while groups III - V were treated i.p. with 60 mg/kg IFO in 0.3mL water (free-IFO); 0.3 mL NE (SAGE-NANO), and 60 mg/kg IFO in 0.3 mL SAGE-NANO (SAGE-IFO), respectively. The treatments were administered for three days. Results: Treatment with 60 mg/kg bwt IFO (free-IFO) significantly elevated the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT, p < 0.05). However, subsequent treatment with SAGE-IFO significantly reduced the activity of these liver enzymes (p < 0.05). The concentration of reduced glutathione (GSH) as well as the activities of catalase and glutathione reductase (GR) significantly increased, while malondialdehyde (MDA) level decreased significantly in SAGE-IFO group, when compared with free-IFO group (p < 0.05). Treatment with SAGE-IFO significantly restored white blood cell (WBC) count and platelet levels which were altered by free-IFO (p < 0.05). Conclusion: The results obtained in this study suggest that loading IFO in sage oil-NE greatly reduces its hepatotoxicity and hematotoxicity.

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Antioxidant and antineoplastic activities of roots of Hibiscus sabdariffa Linn. Against Ehrlich ascites carcinoma cells

Clinical Phytoscience ◽

10.1186/s40816-019-0147-6 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Rumana Yesmin Hasi ◽

Hanif Ali ◽

Majidul Islam ◽

Rowshanul Habib ◽

Mohammed A. Satter ◽

...

Keyword(s):

Tumor Cell ◽

Body Weight Gain ◽

Hematological Parameters ◽

Ehrlich Ascites Carcinoma ◽

Fluorescence Microscope ◽

Carcinoma Cells ◽

Total Phenolic ◽

Hibiscus Sabdariffa ◽

Ehrlich Ascites ◽

Eac Cells

Abstract Background The goal of this study was to explore the inherent antioxidant and antineoplastic activities of methanolic extract of the roots of Hibiscus sabdariffa (MEHSR). Methods The dried coarse powder of roots of Hibiscus sabdariffa was subjected to methanolic extraction. Here in vitro methods were used to determine the various types of phytochemical content and antioxidant activity of MEHSR as well as its cytotoxic effect against Ehrlich ascites carcinoma (EAC) cells. In vivo, antineoplastic activity of MEHSR against EAC cells was also evaluated by determining the viable tumor cell count, survival time, body weight gain, hematological profiles of experimental mice along with observing morphological changes of EAC cells by fluorescence microscope. Analysis of the chemical composition of MEHSR was carried out using GC-MS. Results Total phenolic and flavonoid contents of MEHSR were found to be 143.36 and 82.81 mg/g of extract in terms of gallic acid and catechin equivalent, respectively. The MEHSR exhibited very good scavenging property on DPPH (IC50: 13.37 μg/mL) and ABTS (IC50: 18.88 μg/mL) radicals in respect to nitric oxide (IC50: 72.82 μg/mL) radical and lipid peroxidation (IC50: 75.78 μg/mL) inhibition. MEHSR was found to induce Ehrlich ascites carcinoma (EAC) cell death at a dose dependent fashion. At dose 10 mg/kg, MEHSR significantly inhibited tumor cell growth rate (62.24%; p < 0.05), decreased tumor weight (57.81%; p < 0.05), increased life span (38.97%) compared to the untreated control mice. MEHSR also restored all hematological parameters of EAC-bearing mice towards normal level. Furthermore, administration of MEHSR induced apoptosis of EAC cells as observed in Hoechst 33342 stained cells under fluorescence microscope. Arachidic acid (49.18%), oleic acid (36.36%) and octadecanoic acid (14.47%) were identified as the major components of MEHSR by GC-MS analysis. Conclusion In a nutshell, our findings proposed that MEHSR may possess promising antioxidant and antineoplastic efficacy against Ehrlich ascites carcinoma cells by induction of cell apoptosis. Therefore, it might be a potent and novel candidate for anticancer therapy.

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