scholarly journals In vitro plant regeneration of potato (Solanum tuberosum L.) at the rate of different hormonal concentration

2015 ◽  
Vol 1 (2) ◽  
pp. 297-303 ◽  
Author(s):  
Sayeed Shahriyar ◽  
Soleh Akram ◽  
Koushik Khan ◽  
Md Faruk Miya ◽  
Md Abdur Rauf Sarkar
Keyword(s):  
Acetic Acid ◽  
Solanum Tuberosum ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Average Length ◽  
Root Induction ◽  
Skoog Medium ◽  
Root Regeneration ◽  
Murashige And Skoog Medium

One of the goals of the experiment is to standardization of HgCl2 treatment for explants sterilization. The objectives also include developing a reproducible cost effective protocol for large scale production of Solanum tuberosum of Cardinal variety plantlets from selectively better clones through plant in vitro propagation methods. Selection of growth regulators for proper multiple shoots regeneration, elongation and root induction. To produce genetically uniform plantlets within a short time capable surviving in natural condition raised in in vitro environment. Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on Murashige and Skoog medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl2 (0.1%) for 2 minutes was found to be most effective for complete destroying of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Maximum number of shoot per culture (17) was recorded and it also obtained the highest average length of the shoot (5cm) in Murashige and Skoog medium containing no hormone. On the other hand 6-benzyl amino purine (0.2mg/l) in 3 media showed the highest rate of shoot multiplication (73%) and the highest average length (4cm). In case of Gibberellic acid (0.1mg/l) in Murashige and Skoog media showed its highest rate of shoot regeneration (82%) and the highest average length (4.5cm). From the overall experiment it was observed that shoot tips are more responsive for micro propagation. In root induction Murashige and Skoog medium supplemented with different concentration (0.5, 1, 1.5 and 2mg/l) of indol-3-acetic acid and kinetin. Indol-3-acetic acid and kinetin (1.5+1.5 mg/l) showed its lowest rate of root regeneration (40%) and the average length of the root (1.5 cm). On the contrary Murashige and Skoog medium with no hormone showed the rate of root regeneration (96%) and the highest average length of the root (2.5 cm). The supplemented Murashige and Skoog media with no hormone showed the best performance for root regeneration.Asian J. Med. Biol. Res. June 2015, 1(2): 297-303

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

scholarly journals Plant Regeneration of Pampas Grass from Immature Inflorescences Cultured in Vitro

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 841-843 ◽  
Author(s):  
C.D. Robacker ◽  
W.L. Corley
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Developmental Stage ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Basal Medium ◽  
Cortaderia Selloana ◽  
Dichlorophenoxy Acetic Acid

A micropropagation system to obtain plants from inflorescences of pampas grass (Cortaderia selloana Schult. `Pumila') was developed. Factors examined included developmental stage of inflorescence cultured and growth regulator combinations and concentrations that support explant establishment, shoot regeneration, and rooting. Immature inflorescences ≈300 mm long formed many shoot primordia when initially cultured on Murashige and Skoog basal medium containing 4.5 μm 2,4-D and 8.9 μm BA and subcultured to medium with 0.4 μm 2,4-D and 4.4 μm BA. Thereafter, monthly transfer to a medium without growth regulators yielded about three shoots per tube per month for more than 6 months. Most shoots rooted spontaneously and were easily hardened to greenhouse conditions. Field-tested plants flowered within 2 years and nearly all appeared identical to the parent cultivar. With this technique, several thousand plants can be obtained from a single inflorescence in 1 year. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA); (2,4-dichlorophenoxy)acetic acid (2,4-D).

scholarly journals Micropropagation of Yellow Passionfruit by Axillary Bud Proliferation

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1276-1277 ◽  
Author(s):  
Joao L.C. Faria ◽  
Juan Segura
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Apices ◽  
Passiflora Edulis ◽  
Multiple Shoot Formation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

A protocol for in vitro propagation in yellow passionfruit (Passiflora edulis F. flavicarpa Deg) has been developed. Shoot apices from aseptically grown seedlings were used as initial explants. Multiple shoot formation was obtained by placing the explants on solidified Murashige and Skoog medium containing BA. Regenerated shoots were rooted on media without growth regulators. Following conventional procedures, plantlets were transferred to soil with more than 90% success. Chemical name used: N-(phenylmethyl)-lH-purin-6-amine (BA).

scholarly journals REGENERATION OF CITRUS VIA SHOOT APECIES

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1112a-1112
Author(s):  
Suzanne M.D. Rogers ◽  
Kalyani Dias ◽  
David Byrne
Keyword(s):  
Growth Regulators ◽  
Shoot Tips ◽  
Sour Orange ◽  
Soil Medium ◽  
Skoog Medium ◽  
Murashige And Skoog Medium

Viral damage is a major problem in citrus. As most citrus are asexually propagated, it is necessary to have an alternative way of regenerating virus-free plants from infected plants. Shoot apicies are the most suitable explant material for this purpose because that part of the plant is virus-free. Fifty sour orange shoot tips and 22 Swingle shoot tips, 1 mm - 1.5 mm long, were excised from in vitro germinated seedlings and cultured on semisolid Murashige and Skoog medium, without growth regulators, containing 0.2 % Gelrite. After 8-10 weeks, shoots and leaves developed in 68'% of the sour orange explants, and in 77% of the Swingle explants. Some explants produced roots, after 11-12 weeks, and could be removed from culture and established in soil medium.

scholarly journals Micropropagation of Cascade Huckleberry, Mountain Huckleberry, and Oval-leaf Bilberry Using Woody Plant Medium and Murashige and Skoog Medium Formulations

2007 ◽  
Vol 17 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Danny L. Barney ◽  
Omar A. Lopez ◽  
Elizabeth King
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Shoot Growth ◽  
Woody Plant ◽  
Skoog Medium ◽  
Average Survival ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
Half Strength

Two concentrations of two in vitro media formulations were evaluated for their effects on survival, shoot growth, and percentage rooting of cascade huckleberry (Vaccinium deliciosum), mountain huckleberry (V. membranaceum), and oval-leaf bilberry (V. ovalifolium). Two-node stem sections from established microshoots were cultured on full- or half-strength modified Murashige and Skoog medium (FSMS and HSMS) or full- or half-strength modified woody plant medium (FSWPM and HSWPM) unamended with plant growth regulators. Cultures were maintained at 21 °C with a 16-hour photoperiod for 98 days. Survival on FSMS was reduced by ≈44% for cascade huckleberry, 63% for mountain huckleberry, and 18% for oval-leaf bilberry compared with average survival on HSMS, HSWPM, and FSWPM. Explants on FSMS also produced new shoot growth having the lowest dry weights, fewest shoots, and shortest shoots of the four media. Explant rooting percentages were also least on FSMS. For cascade huckleberry and oval-leaf bilberry, HSMS, HSWPM, and FSWPM all appeared suitable for general culture. For mountain huckleberry, both woody plant medium formulations produced greater microshoot dry weights, average shoot lengths, and explant rooting percentages compared with HSMS. These results are the first published on micropropagation for cascade huckleberry and oval-leaf bilberry, and provide starting protocols for commercial propagation and further research on micropropagation of these species.

scholarly journals INDUCTION OF ABACA BANANA ROOTS BY IN VITRO USING KINDS OF MEDIA AND THIAMIN

Agrivet ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Rina Srilestari ◽  
Suwardi Suwardi
Keyword(s):  
Economic Value ◽  
Dry Weight ◽  
Root Induction ◽  
Market Demand ◽  
Fresh Weight ◽  
Skoog Medium ◽  
Randomized Design ◽  
Murashige And Skoog Medium ◽  
Completely Randomized Design

Abaca is a type a banana with high economic value with it stem fiber used in textile and paper industries. As a superior commodity, its number is relatively limited, with the need of a largeplanting area to meet the high market demand. The aim of the research was to observe the abaca banana explants response to various media and Thiamin. The experiment was done at Biotechnology laboratory, UPN “Veteran” Yogyakarta. Treatments were arranged in completely randomized design with 2 factor. The first factor is the growing media: Murashige & Skoog, a half Murashige & Skoog media, Vacint & Went Media and the second factor is the Thiamin concentration: 2 mg/L; 3 mg/L; 4 mg/mL.The results showed there is an interactions on the parameters of planlet height, number of lenghth of root in the combination of Murashige and Skoog and thiamin 3 mg/L medium. Murashige and Skoog medium produced the highest fresh weight and dry weight and Thiamin concentration 3 mg/L produced fresh and dry weight in the highestKey words: abaka, root induction, various media, thiamin

scholarly journals An Effective Protocol for Carnation (Dianthus caryophyllus L.) cv. 'Geolei' Explants Sterilization for Successful Callusing and Shoot Regeneration

2021 ◽  
pp. 230-238
Author(s):  
Ujjwal Sirohi ◽  
Swati Sharma ◽  
Mukesh Kumar ◽  
R. S. Sengar ◽  
L. K. Gangwar ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Sodium Hypochlorite ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Mercuric Chloride ◽  
Dianthus Caryophyllus ◽  
Leaf Explants ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Root Induction

Carnation is a popular floricultural crop grown widely for its attractive cut flowers. Micro-propagation can be used to create large-scale carnation output. For growth and development, plants require some necessary nutrients as well as growth regulators. Due to the importance of carnation, the present work is carried out using leaf and nodal segments to examine the potential of several plant growth regulators for in vitro callus formation and adventitious shoot regeneration. Explants were sterilized properly with bavistin, sodium hypochlorite and mercuric chloride. The minor contaminated cultures were created by consecutively treating the explants with 0.25% bavistin, 0.50% sodium hypochlorite, and 0.1% mercuric chloride for ten, fifteen, and two minutes. MS media with 2.5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) in combination with 0.75 mg/l naphthalene acetic acid (NAA) resulted in the maximum callus induction (90.47%) from leaf explants. Maximum shoots (76.47%) were produced in MS media supplemented with 2.0 mg/l Thidiazuron (TDZ) + 0.25 mg/l NAA. NAA at 1.25 mg/l was most efficient for maximum root induction (83.32%). In the present study, an effective protocol of carnation explants sterilization was optimized for successful callusing and shoot regeneration.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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