scholarly journals INDUCTION OF ABACA BANANA ROOTS BY IN VITRO USING KINDS OF MEDIA AND THIAMIN

Agrivet ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Rina Srilestari ◽  
Suwardi Suwardi
Keyword(s):  
Economic Value ◽  
Dry Weight ◽  
Root Induction ◽  
Market Demand ◽  
Fresh Weight ◽  
Skoog Medium ◽  
Randomized Design ◽  
Murashige And Skoog Medium ◽  
Completely Randomized Design

Abaca is a type a banana with high economic value with it stem fiber used in textile and paper industries. As a superior commodity, its number is relatively limited, with the need of a largeplanting area to meet the high market demand. The aim of the research was to observe the abaca banana explants response to various media and Thiamin. The experiment was done at Biotechnology laboratory, UPN “Veteran” Yogyakarta. Treatments were arranged in completely randomized design with 2 factor. The first factor is the growing media: Murashige & Skoog, a half Murashige & Skoog media, Vacint & Went Media and the second factor is the Thiamin concentration: 2 mg/L; 3 mg/L; 4 mg/mL.The results showed there is an interactions on the parameters of planlet height, number of lenghth of root in the combination of Murashige and Skoog and thiamin 3 mg/L medium. Murashige and Skoog medium produced the highest fresh weight and dry weight and Thiamin concentration 3 mg/L produced fresh and dry weight in the highestKey words: abaka, root induction, various media, thiamin

scholarly journals Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

2020 ◽  
Vol 22 (1) ◽  
pp. 39
Author(s):  
Amalina Wahyuni ◽  
Benni Satria ◽  
Aprizal Zainal
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Economic Value ◽  
Market Demand ◽  
Compact Structure ◽  
Randomized Design ◽  
Aquilaria Malaccensis ◽  
Completely Randomized Design ◽  
Range Test

<p>Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact.  Yellow callus and compact structure could be utilized for organogenesis.</p>

scholarly journals Nepenthes mirabilis (Lour.) Druce Planlet at a Various Levels of Murashige & Skoog Medium Density In Vitro

2019 ◽  
Vol 6 (2) ◽  
pp. 18-22
Author(s):  
Nadia Fakhriyati Arfa ◽  
Endang Nurcahyani ◽  
Zulkifli Zulkifli ◽  
Tundjung Tripeni Handayani
Keyword(s):  
Homogeneity Test ◽  
Medium Density ◽  
Significance Level ◽  
Tukey Test ◽  
Skoog Medium ◽  
Randomized Design ◽  
Murashige And Skoog Medium ◽  
Murashige Skoog ◽  
Completely Randomized Design

This study aims to determine the variation of the stomata index of the Kantong Semar (Nepenthes mirabilis) planlet at a various medium density of the Murashige and Skoog. This study used a Completely Randomized Design using one factor (medium density of the Murashige and Skoog). We used 5 levels of medium density, i.e.: 1/16 MS, 1/8 MS, 1/4 MS, 1/2 MS, and MS. Homogeneity test used Levene’s test of 5% significance level, then analysis of variance is carried out at 5% significance level and followed by Tukey test at 5% significance level. The results showed that the lower the level of Murashige and Skoog medium density on the Nepenthes mirabilis plantlet, the stomata index also increased.

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals Pengaruh Campuran Pasir dan Serat Aren pada Kailan (Brassica oleraceae) dengan Hidroponik Substrat

2014 ◽  
Vol 16 (1) ◽  
pp. 19
Author(s):  
Samanhudi Samanhudi ◽  
Dwi Hardjoko ◽  
Riandy Adhitya
Keyword(s):  
Wood Fiber ◽  
Dry Weight ◽  
Fresh Weight ◽  
Randomized Design ◽  
The Media ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Brassica Oleraceae ◽  
Media Mix ◽  
Leaf Greenness

<p>Wood fiber waste of arenga produced from starch palm industry. It is not handled properly so that could be potentially cause problems for the surrounding community. Wood fiber waste of arenga is potentially used as a medium for hydroponics. The waste can be utilized to get a new composition of hydroponic media. This study aims to determine the effect of a mixture of sand with arenga fiber waste. This research was carried out by using a mixture of sand and arenga wood fiber waste as a medium. This is an experimental study using a completely randomized design. This study aims to determine the effect of a mixture of sand and arenga fiber waste for kailan growth. The results showed that the mixture of sand and arenga wood fiber waste has no effect on kailan growth. The combination of mixtures of sand and arenga fiber waste showed no interaction. Observations on plants such as plant height, number of leaves, leaf greenness levels, the fresh weight of crop stover and stover dry weight of the plant, showed a row of the highest results in the media mix washed sand, washed sand and rice husk</p>

scholarly journals Respon Pertumbuhan Stek Anggur (Vitis vinifera L.) terhadap Pemberian Beberapa Konsentrasi Ekstrak Bawang Merah (Allium ascalonicum L.)

Akta Agrosia ◽  
2016 ◽  
Vol 19 (1) ◽  
pp. 20-27
Author(s):  
Tri Utami ◽  
Hermansyah Hermansyah ◽  
Merakati Handajaningsih
Keyword(s):  
Dry Weight ◽  
Optimum Concentration ◽  
Vitis Vinifera L ◽  
Fresh Weight ◽  
Shoot Height ◽  
Extract Concentration ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
The City

The purpose of this study was to determine the optimum concentration of shallot extract to the growth of grape cuttings grape cuttings. This study was conducted from September to December 2015 in the city of Bengkulu. This research used Completely Randomized Design with a single factor and five replications. Treatment concentrations of 0% (control), shallot extract concentration of 25%, 75% and 100%. The variables measured were the time to sprout, shoot height, the number of leaves, number of roots, fresh weight and dry weight of cuttings. The results showed that the concentration of shallot extract 100% concentration enhances the growth of grape cutting, time to sprout, and the concentration of extract 50% increase shoot height and number of leaves on grape cuttings.

scholarly journals Optimization of in vitro rooting protocol for tomato (Lycopersicon esculentum mill.) varieties

2020 ◽  
Vol 12 (3) ◽  
pp. 365-371
Author(s):  
Endalkachew Baye ◽  
Temesgen Matewos ◽  
Derbew Belew
Keyword(s):  
Lycopersicon Esculentum ◽  
Root Length ◽  
In Vitro Rooting ◽  
Root Induction ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill ◽  
Randomized Design ◽  
Optimized Protocol ◽  
Completely Randomized Design

In vitro rooting of micropropagated shoots were carried out with the aim of evaluating the root induction responses of two tomatoes (Lycopersicon esculentum MILL) varieties (Gelilema and Chali) using Indole -3- butyric acid (IBA). Seven levels of IBA (0.0, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 mg L-1) were used in a completely randomized design (CRD) in factorial combinations (seven level of IBA*two varieties) with three replications.  After the plantlets were kept in the rooting media for three weeks, data on rooting percentage, number of roots/shoots and root length in cm were collected. The analysis of variance showed that the interaction of IBA*Var was highly significantly different for rooting percentage, a number of roots/shoot and root length at p<0.01. The highest rooting percentage (100.00±0.00), number of roots/shoot (14.20±0.35) and root length (10.7±0.29) were received from Chali on free Murashige and Skoog medium (MS). At the same time, the lowest percentage of rooting (11.11±0.00), number of roots/shoot (0.887±0.19) and root length (1.00±0.00 cm) were obtained from Gelilema on MS+1.5mg/l IBA. For acclimatization, the in vitro rooted shoots were transplanted into plastic pots containing a mixture of oven sterilized soil and sand at a ratio of 2:1. After three weeks, a survival rate of 67.7% for Chali and 58.1% for Gelilema was obtained. From the above result, it can be concluded that free MS medium was the best for in vitro rooting of the two tomato varieties. The optimized protocol will be useful for rapid in vitro multiplication of the two tomato varieties.

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals PENGARUH KOMBINASI PUPUK ORGANIK DAN PUPUK HAYATI TERHADAP PERTUMBUHAN, PRODUKSI DAN MUTU PEGAGAN

2021 ◽  
Vol 32 (1) ◽  
pp. 23
Author(s):  
Rahma Widyastuti ◽  
Nurul Husniyati Listyana ◽  
Erri Setyo Hartanto
Keyword(s):  
Dry Weight ◽  
Growth And Yield ◽  
Stem Length ◽  
Fresh Weight ◽  
Chemical Fertilizers ◽  
Cultivation Method ◽  
Organic Cultivation ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<em>Gotu kola is one of the medicinal plants that has begun to be cultivated by the Indonesian people. It’s cultivation needs to follow the GAP to produce the qualified yield, including the use of fertilizers. Biofertilizers have been widely developed to reduce adverse effects of chemical fertilizers to the environment, thus an organic cultivation method can be an alternative. The aim of this study was to determine the effect of biofertilizer on growth and yield of gotu kola. This research was an experimental study with a completely randomized design (CRD) using 8</em> <em>planting media treatments consisting of (a) Soil + manure + dolomite; (b) Soil manure + 1 g biofertilizer; (c) Soil + dolomite + 1g biofertilizer; (d) Soil + manure + 3 biofertilizer; (e) Soil + dolomite + 3 biofertilizer; (f) Soil + manure + 5 g biofertilizer; (g)  Soil + dolomite + 5 g  biofertilizer;  and  (h)  Soil +  3 g</em> <em>biofertilizer. Parameters observed were leaf area, stem length, number of leaves, number of tillers, fresh weight, dry weight and asiaticoside content. The results showed that combination treatment of biofertilizer (1 g) with dolomite (30 g) gives better effect in increasing the number of tillers and number of leaves, while the combination of biofertilizer (5 g) and dolomite (30 g) is the best for asiaticoside levels in gotu cola.</em>

scholarly journals Pengaruh Jenis Pupuk dan Tingkat Arbuskular Mikoriza terhadap Pertumbuhan dan Hasil Jahe (Zingiber officinale)

2014 ◽  
Vol 16 (2) ◽  
pp. 44
Author(s):  
Dhimas Taufika Putra ◽  
Samanhudi Samanhudi ◽  
Purwanto Purwanto
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Zingiber Officinale ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Fresh Weight ◽  
Randomized Design ◽  
Medical Plant ◽  
Completely Randomized Design

<p>Ginger (<em>Zingiber officinale</em>) is one kind of plants to be processed as traditional medicine, drinks, and spices. the smallest type of ginger that has many benefits as medical plant. Demand for ginger plants growing in the community is increasing. Additions of manure and arbuscular mychorrhizal fungi (AMF) can increase the quality and quantity of ginger. This was conducted to obtain the kinds of manure and levels of AMF to ginger to produce optimum growth and yield. This research used a completely randomized design (CRD) consisting of two treatment factors, namely planting medium and arbuscular mycorrhizal fungi (AMF). Results were analyzed by analysis of variance and if there is a real difference continued DMRT 5% level. The results showed that the differences in the kinds of manure and the levels of AMF increase growth and yield of ginger. Chicken manure application showed the highest results in the fresh weight of stover plant, dry weight of plant stover, fresh weight of plant rhizomes, and dry weight of the rhizomes. Whereas the additions of AMF 15 grams per plant in zeolite showed the highest results in the number of plant tillers, dry weight of plant stover, fresh weight of plant rhizomes, and dry weight of the rhizomes.</p>

scholarly journals Production of Brachiaria decumbens Grass at Various Levels of Drought Stress Treatment

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
A F A Putra ◽  
N D Hanafi ◽  
Hamdan ◽  
M Tafsin ◽  
R E Mirwandhono
Keyword(s):  
Drought Stress ◽  
Plant Height ◽  
Field Capacity ◽  
Dry Weight ◽  
Water Volume ◽  
Fresh Weight ◽  
Brachiaria Decumbens ◽  
Stress Treatment ◽  
Randomized Design ◽  
Completely Randomized Design

This study aims to determine effects of production of Brachiaria decumbens grass on different drought stress treatments. Research was conducted at the Greenhouse Faculty of Agriculture University of North Sumatera from Desember 2016 until April 2017. The design used was Completely Randomized Design (CRD), with three treatments and seven replications. The treatment consisted of three levels of drought stress which were control (100% field capacity=fc), medium drought stress (25% fc), and heavy drought stress (50% fc). The variable were fresh weight production, dry weight production and plant height. The results showed that drought stress treatment control (100% fc) significantly (P<0,05) affect fresh weight production, dry weight production, but not plant height. It is concluded that increasing water volume of field capacity increase production Brachiaria decumbens grass.

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