scholarly journals Detection of avian reovirus antibodies in layer birds of small scale commercial farms in Dinajpur district of Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 612-621 ◽  
Author(s):  
Abdus Salam ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Farzana Afroz
Keyword(s):  
Antibody Titer ◽  
Specific Antibody ◽  
Age Groups ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Avian Reovirus ◽  
Serum Samples ◽  
Aged Group ◽  
Test Kit

The present study was conducted on layer birds of different age groups to determine specific antibody titer level against avian reovirus (ARV) by indirect enzyme linked immunosorbent assay (iELISA) at Dinajpur district of Bangladesh. This study showed that ARV specific antibody positive cases were 84 out of 90 blood serum samples and the highest antibody titer was 26120 and lowest antibody titer was 288. The total 93.33% sera samples were showed positive result. The study showed that 100% sera sample were positive against ARV at 6 weeks of aged group and the highest, lowest and mean antibody titer were 13917, 4895 and 10269 respectively. On the other hand 88.88% sera sample were positive against ARV at 10 weeks of aged group and the highest, lowest and mean antibody titer were 9779, 288 and 5689.89 respectively. The sera sample collected from 14 weeks of aged group showed 88.88% positive and the highest, lowest and mean antibody titer were 11727, 871 and 5250 respectively. The sera sample collected from 18 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 24440, 1234 and 12648.89 respectively. The sera sample collected from 22 weeks of aged group were 100% positive against ARV and the highest, lowest and mean antibody titer were 26120, 1752 and 11373.89 respectively. The sera sample collected from 26 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 8566, 1630 and 4327.44 respectively. The sera sample collected from 30 weeks of aged group showed 100% positive against ARV and the highest, lowest and mean antibody titer were 13431, 1989 and 5890.56 respectively. The sera sample collected from 40 weeks of aged group showed 77.77% positive against ARV and the highest, lowest and mean antibody titer were 14618, 433 and 5103.22 respectively. The sera sample collected from 48 weeks of aged group showed 88.88% positive against ARV and the highest, lowest and mean antibody titer were 14553, 957 and 7436.5 respectively. In conclusion it is evident that avian reovirus-specific antibody was successfully detected through commercially available avian reovirus antibody test kit (ELISA kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely ARV.Asian J. Med. Biol. Res. December 2015, 1(3): 612-621

scholarly journals Detection of Specific Antibodies to an Antigenic Mannoprotein for Diagnosis of Penicillium marneffeiPenicilliosis

1998 ◽  
Vol 36 (10) ◽  
pp. 3028-3031 ◽  
Author(s):  
Liang Cao ◽  
Da-Liang Chen ◽  
Cindy Lee ◽  
Che-Man Chan ◽  
King-Man Chan ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Blood Donors ◽  
Antibody Test ◽  
High Specificity ◽  
Pathogenic Fungi ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Immunodeficiency Virus ◽  
Positive Results

The disseminated and progressive fungal disease Penicillium marneffei penicilliosis is one of the most common infectious diseases in AIDS patients in Southeast Asia. To diagnose systemic penicilliosis, we developed an enzyme-linked immunosorbent assay (ELISA)-based antibody test with Mp1p, a purified recombinant antigenic mannoprotein of P. marneffei. Evaluation of the test with guinea pig sera against P. marneffei and other pathogenic fungi indicated that this assay was specific for P. marneffei. Clinical evaluation revealed that high levels of specific antibody were detected in two immunocompetent penicilliosis patients. Furthermore, approximately 80% (14 of 17) of the documented penicilliosis patients with human immunodeficiency virus tested positive for the specific antibody. No false-positive results were found for serum samples from 90 healthy blood donors, 20 patients with typhoid fever, and 55 patients with tuberculosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Mp1p antibody can be of significant value as a diagnostic for penicilliosis.

scholarly journals Seroprevalence of Toxoplasma gondii infection in domestic pigs reared under different management systems in Zimbabwe

2005 ◽  
Vol 72 (3) ◽  
Author(s):  
T. Hove ◽  
P. Lind ◽  
S. Mukaratirwa
Keyword(s):  
Toxoplasma Gondii ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Scale ◽  
Serum Samples ◽  
Serum Dilution ◽  
Domestic Pigs ◽  
Commercial Farms ◽  
Fattening Pigs

Serum samples from 474 domestic pigs (Sus scrofa) from Zimbabwe were tested for anti-Toxoplasma gondii IgG antibodies using the indirect fluorescent antibody test. The results showed that T. gondii infection is widespread in Zimbabwean pigs. Seroprevalence was lowest in fattening pigs from large and small-scale commercial farms that practise good hygiene (19.75 % of 238) and highest in backyard scavenging pigs (35.71 % of 70). Only 11.7 % (11) of the 127 positive samples had titres of > 1:400 and nine (81.82 %) of these 11 originated from pigs reared under poor hygienic conditions. A prevalence of 3.51 % was found in the same group of fattening pigs using an indirect IgG enzyme-linked immunosorbent assay at the single serum dilution of 1:400. The serosurvey shows the importance of modern intensive husbandry systems in reducing the prevalences of T. gondii infection in domestic pigs.

scholarly journals Detection of Specific type-A Antigen and Specific Antibody against Avian Influenza virus in Native Duck at Netrokona district of Bangladesh

2016 ◽  
Vol 4 (2) ◽  
pp. 5-10
Author(s):  
MM Mafizul Islam ◽  
Mir Rowshan Akter ◽  
Md Mostafizer Rahman ◽  
Md Atiqul Haque ◽  
Md Karim Uddin ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Positive Result ◽  
Avian Influenza Virus ◽  
Antibody Titer ◽  
Specific Antibody ◽  
Type A ◽  
Aged Group ◽  
Influenza Type ◽  
Test Kit

The present study was conducted on unvaccinated native ducks of different age groups to determine specific antibody titer level against Avian Influenza virus (AIV) by indirect Enzyme Linked Immunosorbent Assay (iELISA) and to detect avian influenza type A virus antigen by rapid AIV antigen test kit at Netrokona district of Bangladesh. This study showed that AIV specific antibody positive cases were 78 out of 90 blood serum samples and the highest antibody titer was 2323 and lowest antibody titer was 256. The total 86.67% sera samples were showed positive result. The study showed that 66.66% sera sample were positive against AIV at 3-4 month of aged group and the highest, lowest and mean antibody titer were 1428, 256 and 906.3 respectively. On the other hand 78% sera sample were positive against AIV at 5-6 month aged group and the highest, lowest and mean antibody titer were 1675 , 451 and 1083.6 respectively. The sera sample collected from 7-8 month aged group showed 88.9% positive and the highest, lowest and mean antibody titer were 1857, 578 and 1285.5 respectively. The sera sample collected from 9-10 month of aged group showed 100% positive against AIV and the highest, lowest and mean antibody titer were 197l, 638 and 1571.5 respectively .The sera sample collected from duck of ?11 month aged group were 100% positive against AIV and the highest, lowest and mean antibody titer were 2323, 1423 and 1813.7 respectively. Tracheal and cloacal swabs from ducks with antibody titer more than 1813.778 were tested for the avian influenza type A antigen by Anigen Rapid AIV Ag test kit. The above sample showed 20% positive result. In conclusion it is evident that Avian influenza virus-specific antibody was successfully detected through commercially available Avian influenza virus antibody test kit (ELISA Kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely AIV.

scholarly journals Serological detection of avian reovirus in different poultry flocks of Gazipur and Mymensingh districts of Bangladesh

2019 ◽  
Vol 12 (7) ◽  
pp. 1126-1131 ◽  
Author(s):  
Syeda Farjana Neepa ◽  
Zobayda Farzana Haque ◽  
Abdullah Al Momen Sabuj ◽  
Md Alimul Islam ◽  
Sukumar Saha
Keyword(s):  
Large Scale ◽  
Serological Test ◽  
Age Groups ◽  
Vaccination Status ◽  
Enzyme Linked Immunosorbent Assay ◽  
Avian Reovirus ◽  
Wing Vein ◽  
Broiler Breeder ◽  
Blood Samples ◽  
Test Kit

Background and Aim: Avian reovirus (ARV) is a constraint to poultry industry in Bangladesh as a cause of several diseases in chickens, especially in broiler. However, the actual status of the viral infection is not known because the large-scale study is not conducted in this country. Therefore, this study aimed to check the presence and distribution of ARV-specific antibody in respect to area, types of chickens (broiler breeder, broiler, and layer), vaccination status, and age of chickens in Gazipur and Mymensingh districts of Bangladesh. Materials and Methods: A total of 276 chickens' blood samples were collected from two well-organized broiler breeder stock, seven broiler farms, and five layer farms located at two districts, namely Gazipur and Mymensingh of Bangladesh. Blood samples were collected from wing vein of the apparently healthy chickens using 3 ml of syringe and serum was harvested by keeping the syringe at room temperature in slanting position. The sera were transferred to the laboratory by maintaining the cool chain and further processing was performed by indirect enzyme-linked immunosorbent assay using ARV antibody test kit. Results: The results of serological test revealed that an average of 39.5% seropositive against ARV was recorded in chickens of Gazipur and Mymensingh districts. Among these, chickens of Gazipur district had the highest seropositivity of 50.5% than Mymensingh (30.7%). With respect to vaccination status, the seropositivity of vaccinated chickens in both areas was 100% and non-vaccinated chickens was 50.5% in Gazipur and 30.7% in Mymensingh district, respectively. However, regarding age groups, the seropositivity was higher in the age of 4-6 weeks (64.5%). Conclusion: The present serological findings showed a higher prevalence of ARV-specific antibodies in broiler birds. It indicates that the poultry industries of Bangladesh are contaminated with ARV which may naturally be transmitted to chickens either vertically or horizontally.

scholarly journals Development and Validation of A Competitive ELISA Based on Virus-Like Particles of Serotype Senecavirus A to Detect Serum Antibodies

2020 ◽  
Author(s):  
Manyuan Bai ◽  
Rui Wang ◽  
Shiqi Sun ◽  
Yun Zhang ◽  
Hu Dong ◽  
...  
Keyword(s):  
Expression System ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Virus Like Particles ◽  
Specificity And Sensitivity ◽  
Indirect Immunofluorescent Assay ◽  
Viral Particles ◽  
Test Kit ◽  
Development And Validation

Abstract Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an Escherichia coli expression system as the coating antigens. VLPs have better safety and immunogenicity than intact viral particles or peptides. The VLP-based cELISA was used to test 342 serum samples collected from different pig farms, and the results showed that its specificity and sensitivity were 100% and 94%, respectively. The consistency rates of cELISA with the BIOSTONE AsurDx™ Senecavirus A (SVA) Antibody Test Kit and an indirect immunofluorescent assay were 90.0% and 94.2%, respectively. Therefore, this VLP-based cELISA can be effectively and reliably used for the detection and discrimination of SVA infection in serum samples.

scholarly journals Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Manyuan Bai ◽  
Rui Wang ◽  
Shiqi Sun ◽  
Yun Zhang ◽  
Hu Dong ◽  
...  
Keyword(s):  
Expression System ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Virus Like Particles ◽  
Specificity And Sensitivity ◽  
Indirect Immunofluorescent Assay ◽  
Viral Particles ◽  
Test Kit ◽  
Development And Validation

AbstractVirus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens. VLPs have better safety and immunogenicity than intact viral particles or peptides. The VLPs-based cELISA was used to test 342 serum samples collected from different pig farms, and the results showed that its specificity and sensitivity were 100% and 94%, respectively. The consistency rates of cELISA with the BIOSTONE AsurDx™ Senecavirus A (SVA) Antibody Test Kit and an indirect immunofluorescent assay were 90.0% and 94.2%, respectively. Therefore, this VLPs-based cELISA can be effectively and reliably used for the detection and discrimination of SVA infection in serum samples.

scholarly journals Antibodies anti-trypanosomatides in domestic cats in Paraná: who is at highest risk of infection?

2018 ◽  
Vol 27 (2) ◽  
pp. 232-236 ◽  
Author(s):  
Andressa Maria Rorato Nascimento de Matos ◽  
Eloiza Teles Caldart ◽  
Fernanda Pinto Ferreira ◽  
Keila Clarine Monteiro ◽  
Marielen de Souza ◽  
...  
Keyword(s):  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
Serum Samples ◽  
Animal Group ◽  
Animal Protection ◽  
Leishmania Spp ◽  
In Series ◽  
Different Populations ◽  
Simple Logistic

Abstract The aim of this study were to detect antibodies anti-Leishmania spp. and anti-Trypanosoma cruzi in two different populations of domestic cats (Felis catus domesticus) from North Paraná referred for surgical castration and to determine which characteristics of the animals studied may be associated with seropositivity. Serum samples from 679 cats were analyzed using enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT) in series. Associations between age, sex, race, year of care and animal group were verified using the simple logistic regression. Percentage of 8.5% (58/679) of cats were positive for Leishmania spp. and 7.6% (51/673) for T. cruzi by the tests ELISA and IFAT. Animals collected by non-governmental animal protection organizations presented more seropositivity for Leishmania spp. (p<0.0001). Results shown that Leishmania spp. and T. cruzi are present in domestic cats in the northern part of the state of Paraná, as well as, owners of non-governmental animal protection organizations may be more exposed to leishmaniasis when compared to other animal owners evaluated in the present study.

scholarly journals Specificity and Prevalence of Natural Bovine Antimannan Antibodies

1999 ◽  
Vol 6 (6) ◽  
pp. 946-952 ◽  
Author(s):  
Abhay Srinivasan ◽  
Yawei Ni ◽  
Ian Tizard
Keyword(s):  
Age Groups ◽  
Inhibitory Effect ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Serum Samples ◽  
Adult Cattle ◽  
Calcium Dependent ◽  
High Concentrations ◽  
Carbohydrate Components ◽  
A Minor

ABSTRACT Immune responses to the carbohydrate components of microorganisms, mediated both by antibodies and by lectins, are an important part of host defense. In the present experiments, the specificity and presence of natural bovine antibodies against mannan, a common fungal antigen, were examined by enzyme-linked immunosorbent assay (ELISA), usingSaccharomyces cerevisiae mannan as an antigen. The results showed that all serum samples from animals of three age groups (newborn, calf, and adult) tested contained antimannan antibodies, and the titer of these antibodies increased significantly in adults. However, titers among individual adult cattle differed widely. Inhibition assays showed that yeast mannan was the strongest inhibitor.d-Mannose exhibited only a minor inhibitory effect at high concentrations. This suggests that most of these antibodies recognize an oligosaccharide-based epitope(s) different from those recognized by lectins. Cattle possess three serum C-type lectins (collectins) capable of recognizing mannan in a calcium-dependent manner. Addition of EDTA to the reaction did not reduce antibody binding, suggesting that the binding of these antibodies to mannan was not affected by the presence of collectin. The antibodies purified from either calf or adult serum by mannan-Sepharose affinity chromatography consisted of mainly immunoglobulin G (IgG) and a smaller amount of IgM. IgG1 was shown to be the dominant antimannan IgG isotype by isotype-specific ELISA. Together, these results demonstrate the production of natural antimannan antibodies in cattle in an age-dependent manner. These antibodies might be involved in defending the host against mannan-containing pathogens as a specific line of defense in conjunction with the innate response by lectins.

scholarly journals Investigation ofAnaplasma marginaleSeroprevalence in a Traditionally Managed Large California Beef Herd

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Thomas R. Tucker ◽  
Sharif S. Aly ◽  
John Maas ◽  
Josh S. Davy ◽  
Janet E. Foley
Keyword(s):  
Anaplasma Marginale ◽  
Incidence Density ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Cattle Disease ◽  
Angus Cattle ◽  
Increased Risk ◽  
Test Kit ◽  
Incidence Density Rate ◽  
Density Rate

Recent observations by stakeholders suggested that ecosystem changes may be driving an increased incidence of bovine erythrocytic anaplasmosis, resulting in a reemerging cattle disease in California. The objective of this prospective cohort study was to estimate the incidence ofAnaplasma marginaleinfection using seroconversion in a northern California beef cattle herd. A total of 143 Black Angus cattle (106 prebreeding heifers and 37 cows) were enrolled in the study. Serum samples were collected to determineAnaplasma marginaleseroprevalence using a commercially available competitive enzyme-linked immunosorbent assay test kit. Repeat sampling was performed in seronegative animals to determine the incidence density rate from March through September (2013). Seroprevalence of heifers was significantly lower than that of cows at the beginning of the study (P<0.001) but not at study completion (P=0.075). Incidence density rate ofAnaplasma marginaleinfection was 8.17 (95% confidence interval: 6.04, 10.81) cases per 1000 cow-days during the study period. Study cattle becameAnaplasma marginaleseropositive and likely carriers protected from severe clinical disease that might have occurred had they been first infected as mature adults. No evidence was found within this herd to suggest increased risk for clinical bovine erythrocytic anaplasmosis.

scholarly journals West Nile Virus and Related Flavivirus in European Wild Boar (Sus scrofa), Latium Region, Italy: A Retrospective Study

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 494
Author(s):  
Angela Petruccelli ◽  
Tiziana Zottola ◽  
Gianmarco Ferrara ◽  
Valentina Iovane ◽  
Cristina Di Russo ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Boar ◽  
Specific Antibody ◽  
Central Italy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Serum Samples ◽  
West Nile ◽  
Wild Boars ◽  
European Wild Boar

Background: A retrospective sero-survey for evidence of West Nile virus (WNV) infection in European wild boar (Sus scorfa) was conducted in the Latium region, Italy, on stored serum samples of the period November 2011 to January 2012. Methods: Sera were collected from 168 European wild boars and screened for antibodies to WNV and other Flaviviruses by competitive enzyme linked immunosorbent assay (cELISA). All sera positive for Flavivirus antibodies by cELISA were further examined by virus neutralization test (VNT). To test the presence of Flavivirus RNA in samples, an RT-PCR was performed using a pan-Flavivirus primers pair. Results: Thirteen wild boars (7.73%) were seropositive for Flaviviruses. The hemolysis of serum samples limited the interpretation of the VNT for 7 samples, confirming the presence of specific antibody against WNV in a single European wild boar serum sample. The presence of ELISA positive/VNT negative samples suggests the occurrence of non-neutralizing antibodies against WNV or other antigen-related Flaviviruses. No samples resulted positive for Flavivirus by RT-PCR assay. Conclusion: Although a moderately high percentage of animals with specific antibody for WNV has been detected in wild boar in other surveillance studies in Europe, this has not been reported previously in Italy. Together, these data indicate that European wild boar are exposed to WNV and/or other related-Flavivirus in central Italy and confirm the usefulness of wild ungulates, as suitable Flavivirus sentinels.

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