scholarly journals Genetic relationship among indigenous sheep population of Bangladesh

2019 ◽  
Vol 48 (1) ◽  
pp. 17-22
Author(s):  
GK Deb ◽  
MP Choudhury ◽  
MA Kabir ◽  
MYA Khan ◽  
M Ershaduzzaman ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Microsatellite Markers ◽  
River Basin ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Genetic Group ◽  
Sheep Population ◽  
Blood Samples ◽  
Indigenous Sheep ◽  
Pcr Product

The study was conducted to investigate the genetic relationships among indigenous sheep population of Bangladesh (Barind, Jamuna river basin, Coastal and Garole sheep) using microsatellite markers. A total of 96 blood samples were collected from adult sheep of Barind (24), Jamuna River Basin (24), Coastal (24), Garole(10) and available Chotanagpuri (10) sheep. Chotanagpuri sheep was used as an outgroup population. DNA was extracted from blood samples using QIAGEN DNA Mini extraction kit and was quantified using a nanodrop. FAO recommended 13 labeled microsatellite markers were used for polymerase chain reaction (PCR). PCR product was confirmed by 2% agarose gel electrophoresis and visualized by staining with ethidium bromide.The exact allele sizes in each primer were determined by GeneMaker V1.85 demo. Microsatellite tool kit and Dispan software package were used for calculation of allele frequency, number of alleles per locus, observed and expected heterozygosity and genetic distances (DA). The Dispan software was used to calculate inter-individual genetic distances. These distance values were used to construct an UPGMA tree. Results showed that average number of polymorphic alleles per locus varied from4 in HUJ616 to 12 in MAF70. Observed heterozygosity was also varied from 0.54±0.04 in Coastal to 0.63±0.03 in Barind sheep population. Genetic distance between Jamuna river basin and Barind was lowest (0.01) and between Garole and Costal was highest (0.17). Garoleand Chotonagpuri sheep has higher genetic distance from other three sheep populations. Phylogenetic dendogram showed that sheep of Jamuna river basin and barind were belonged to same genetic group. Whereas, coastal, garole and Nagpur sheeps were shown higher genetic distances from Jamuna river basin and coastal sheep. Considering findings of this study it may be concluded that the Barind and Jamuna river basin sheep belongs to a similar genetic group while, Garole and Coastal sheep are belonging to two distinct genetic groups. Bang. J. Anim. Sci. 2019. 48 (1): 17-22

scholarly journals Genetic diversity in table grapes based on RAPD and microsatellite markers

2011 ◽  
Vol 46 (9) ◽  
pp. 1035-1044 ◽  
Author(s):  
Patrícia Coelho de Souza Leão ◽  
Sérgio Yoshimitsu Motoike
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Relationships ◽  
Similarity Index ◽  
Genetic Distances ◽  
Table Grape ◽  
Group Method ◽  
Molecular Characteristics ◽  
Table Grapes ◽  
Pair Group

The objective of this work was to analyze the genetic diversity of 47 table grape accessions, from the grapevine germplasm bank of Embrapa Semiárido, using 20 RAPD and seven microsatellite markers. Genetic distances between pairs of accessions were obtained based on Jaccard's similarity index for RAPD data and on the arithmetic complement of the weighted index for microsatellite data. The groups were formed according to the Tocher's cluster analysis and to the unweighted pair‑group method with arithmetic mean (UPGMA). The microsatellite markers were more efficient than the RAPD ones in the identification of genetic relationships. Information on the genetic distance, based on molecular characteristics and coupled with the cultivar agronomic performance, allowed for the recommendation of parents for crossings, in order to obtain superior hybrids in segregating populations for the table grape breeding program of Embrapa Semiárido.

scholarly journals Study of genetic differences among Slovak Tsigai populations using microsatellite markers

2009 ◽  
Vol 54 (No. 10) ◽  
pp. 468-474 ◽  
Author(s):  
S. Kusza ◽  
E. Gyarmathy ◽  
J. Dubravska ◽  
I. Nagy ◽  
A. Jávor ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Mating System ◽  
Population Differentiation ◽  
Genetic Relationships ◽  
The Other ◽  
Potential Risks ◽  
Substantial Degree

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of <I>F</I><sub>ST</sub> (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.

scholarly journals Genetic Diversity Based on ISSR Markers of Apple Genotypes in Ardahan/Turkey

2018 ◽  
Vol 10 (4) ◽  
pp. 554-558
Author(s):  
Emre SEVİNDİK ◽  
Hüseyin UYSAL ◽  
Zehra Tuğba MURATHAN
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Uv Light ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Genetic Distances ◽  
The Matrix ◽  
Commercial Kits ◽  
Issr Primers

Within the present study, it was conducted a genetic diversity analysis using ISSR markers for some apple genotypes grown in Ardahan region, Turkey. Total genomic DNA (gDNA) isolation from apple leaves was performed using commercial kits. Five ISSR primers were used to determine the genetic diversity among the genotypes studied. Polymerase Chain Reaction (PCR) was performed with all gDNA samples to produce bands to score. PCR products were run in agarose gel and visualized under UV light. Bands on the gels were scored as “1”, while no bands at the corresponding positions were scored as “0”, to generate the matrix file. Five ISSR primers produced a total of 35 bands, and 20 of them were polymorphic. The polymorphic bands rated approximately 57%. Phylogenetic relationships and genetic distances between the genotypes were calculated by using the PAUP [Phylogenetic Analysis Using Parsimony (and Other Methods)] program.  According to the PAUP data, the closest genetic distance was 0.03704 between ‘Kaburga’ and ‘Japon Apple’ genotypes, while the furthest genetic distance was 0.48148 between ‘Karanfil Apple’ and ‘Sisli Uruset’. The phylogenetic analysis obtained using UPGMA algorithm produced a phylogenetic tree with two clades. The results suggest that ISSR markers are useful tools for determining genetic relationships among apple genotypes.

Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers

2012 ◽  
Vol 92 (4) ◽  
pp. 417-423 ◽  
Author(s):  
Jinjun Li ◽  
Qingyuan Yuan ◽  
Junda Shen ◽  
Zhengrong Tao ◽  
Guoqing Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Differentiation ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Genetic Relationships ◽  
Evolutionary Relationships ◽  
Distance Estimate ◽  
Cluster Analyses ◽  
Nei's Genetic Distance

Li, J., Yuan, Q., Shen, J., Tao, Z., Li, G., Tian, Y., Wang, D., Chen, L. and Lu, L. 2012. Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers. Can. J. Anim. Sci. 92: 417–423. The aim of this study was to determine the genetic diversity and evolutionary relationships among five indigenous Chinese goose breeds and one introduced goose breed using 29 microsatellite markers. A total of 334 distinct alleles were observed across the six breeds, and 45 of the 334 alleles (13.5%) were unique to only one breed. The indigenous geese showed higher diversity in terms of the observed number of alleles per locus (4.48–5.90) and observed heterozygosity (0.46–0.53) compared with the introduced breed (3.97 and 0.29, respectively). The pairwise genetic differentiation (FST) between the six goose breeds ranged from 0.04 between Panshi Grey goose (PS) and Yongkang Grey goose to 0.47 between PS and Landes goose; similarly, Nei's genetic distance varied between 0.25 and 0.75. However, the FST between the indigenous Chinese goose breeds was very small. In addition, genetic distance estimate, phylogenic, and cluster analyses of the genetic relationships and population structure revealed that some indigenous goose breeds had hybridized more frequently, resulting in a loss of genetic distinctiveness.

scholarly journals Assessment of genetic variation in Bapedi sheep using microsatellite markers

2020 ◽  
Vol 50 (2) ◽  
pp. 318-324
Author(s):  
A. Maqhashu ◽  
N.O. Mapholi ◽  
H.A. O’Neill ◽  
K.A. Nephawe ◽  
F.V. Ramukhithi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Microsatellite Markers ◽  
South African ◽  
Agricultural Research ◽  
Germplasm Conservation ◽  
Genetic Distances ◽  
Fixation Index ◽  
Indigenous Sheep ◽  
Agricultural Research Council

This study was conducted to assess genetic variation in Bapedi sheep using 14 microsatellite markers. Blood samples were collected from 174 unrelated Bapedi sheep on six farms in various districts of Limpopo and from the Agricultural Research Council Animal Production Institute (ARC-API) in Gauteng. Genotypes from other South African indigenous sheep, namely Zulu (N = 14), Damara (N = 11), Dorper (N = 8), and Namaqua (N = 11), were included to represent reference populations. The effective number of alleles averaged 5.6 for across the Bapedi flocks and was 4.9 for the reference breeds. Among the Bapedi flocks, the observed heterozygosity (Ho) ranged from 0.56 ± 0.05 to 0.69 ± 0.03 and expected heterozygosity (He) values were between 0.75 ± 0.04 and 0.88 ± 0.01. Thus, there is considerable genetic diversity within the Bapedi sheep populations. However, the fixation index was high, indicating the possibility of inbreeding becoming a problem for these flocks. A neighbour-joining tree was constructed from the estimates of Nei’s genetic distances among flocks. The presence of Bapedi sheep flocks on all of the main branches of the tree along with one of the reference breeds suggests the present-day Bapedi is not an entirely distinct breed and that there are genetic differences between flocks of these South African indigenous sheep. Sustainable breeding and conservation programmes are needed to control inbreeding and to foreclose possible genetic dilution of Bapedi sheep. Keywords: genetic diversity, germplasm conservation, inbreeding, indigenous sheep

scholarly journals Biochemical polymorphisms and genetic relationships between Brazilian and foreign breeds of pigs reared in Brazil

1999 ◽  
Vol 29 (2) ◽  
pp. 319-323 ◽  
Author(s):  
Cláudia Helena Tagliaro ◽  
Maria Helena Lartigau Pereira Franco ◽  
Maria Paula Cruz Schneider ◽  
Benito Guimarães de Brito ◽  
Antonio Stockler Barbosa
Keyword(s):  
Genetic Variability ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
The Other ◽  
Paternity Testing ◽  
Gene Frequencies ◽  
Large White ◽  
Blood Samples ◽  
Biochemical Polymorphisms ◽  
Paternity Exclusion

The genetic variability of 14 protein systems encoded by 15 structural loci was investigated in blood samples of Piau and Caruncho pig breeds. The results were compared with those obtained previously for samples of Landrace, Large White, Duroc and Mouro. The degree of genetic variability obtained for Piau (He=0.114) was similar to that estimated for other breeds reared in Brazil (Landrace, He=0.116; Large White, He=0.119; Duroc, 0.095; Mouro, He= 0.130). Caruncho showed the lowest variability (He= 0.056). The gene frequencies at the polymorphic loci were used to evaluate the usefulness of these systems for paternity testing and the combined probabilities of paternity exclusion were estimated at 58% for the Piau and 36% for the Caruncho breed. Analysis of genetic distances revealed that the greatest similarity observed was between Piau and Landrace (D=0.042). Caruncho showed the greatest divergence among all breeds compared and the distances between this breed and others range from 0.107 (with Landrace) to 0.176 (with Duroc). The tree constructed by UPGMA and Rogers’ Distance gave a topology in which Piau and Mouro joined with the European breeds (Landrace and Large White) whereas Caruncho was separated from all the other breeds. The results of the analysis of the Caruncho samples should be interpreted with caution since the number of animals studied was small.

scholarly journals Microsatellite Markers Developed from `Bluecrop' Reveal Polymorphisms in the Genus Vaccinium and Are Suitable for Cultivar Fingerprinting

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1122B-1122 ◽  
Author(s):  
Peter Boches ◽  
Lisa J. Rowland ◽  
Kim Hummer ◽  
Nahla V. Bassil
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Genetic Relationships ◽  
Distance Measures ◽  
Hybrid Origin ◽  
Pedigree Information ◽  
Species Relationships ◽  
Ssr Loci

Microsatellite markers for blueberry (Vaccinium L.) were created from a preexisting blueberry expressed sequence tag (EST) library of 1305 sequences and a microsatellite-enriched genomic library of 136 clones. Microsatellite primers for 65 EST-containing simple sequence repeats (SSRs) and 29 genomic SSR were initially tested for amplification and polymorphism on agarose gels. Potential usefulness of these SSRs for estimating species relationships in the genus was assessed through cross-species transference of 45 SSR loci and cluster analysis using genetic distance values from five highly polymorphic EST-SSR loci. Cross-species amplification for 45 SSR loci ranged from 17% to 100%, and was 83% on average in nine sections. Cluster analysis of 59 Vaccinium species based on genetic distance measures obtained from 5 EST-SSR loci supported the concept of V. elliotii Chapm. as a genetically distinct diploid highbush species and indicated that V. ashei Reade is of hybrid origin. Twenty EST-SSR and 10 genomic microsatellite loci were used to determine genetic diversity in 72 tetraploid V. corymbosum L. accessions consisting mostly of common cultivars. Unique fingerprints were obtained for all accessions analyzed. Genetic relationships, based on microsatellites, corresponded well with known pedigree information. Most modern cultivars clustered closely together, but southern highbush and northern highbush cultivars were sufficiently differentiated to form distinct clusters. Future use of microsatellites in Vaccinium will help resolve species relationships in the genus, estimate genetic diversity in the National Clonal Germplasm Repository (NCGR) collection, and confirm the identity of clonal germplasm accessions.

scholarly journals Impact of Wide Hybridization on Highbush Blueberry Breeding

2008 ◽  
Vol 133 (3) ◽  
pp. 427-437 ◽  
Author(s):  
Patricio A. Brevis ◽  
Nahla V. Bassil ◽  
James R. Ballington ◽  
James F. Hancock
Keyword(s):  
Microsatellite Markers ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Wide Hybridization ◽  
Highbush Blueberry ◽  
Cultivar Group ◽  
Genetic Base ◽  
Southern Highbush ◽  
Genetic Contributions ◽  
The Impact

The use of interspecific hybridization in blueberry (Vaccinium L. section Cyanococcus Gray) breeding has resulted in the incorporation of novel traits from wild germplasm and the expansion of the geographic limits of highbush blueberry (V. ×corymbosum L.) production. The objectives of this study were: 1) to estimate the impact of wide hybridization on inbreeding, heterozygosity, and coancestry of the cultivated tetraploid highbush blueberry; 2) to establish the usefulness of microsatellite markers in assessing genetic relationships among southern highbush blueberry [SHB (V. ×corymbosum)] cultivars; and 3) to report on the expected genetic contribution of wild Vaccinium clones to SHB cultivars. Pedigrees of 107 highbush blueberry cultivars were used to calculate tetrasomic inbreeding coefficients (F), pedigree-based genetic distances, and expected genetic contributions of wild clones. Genotyping data from 21 single-locus microsatellite markers screened across 68 genotypes were used to calculate heterozygosity and proportion of shared alleles distances (Dsa). The results indicated that the effects of wide hybridization on genetic diversity of cultivated blueberry were lower than previously thought. First, SHB cultivars exhibited similar levels of molecular relatedness as historical northern highbush blueberry [NHB (V. ×corymbosum)] cultivars (median Dsa between pairs of cultivars equals 0.19 in both cultivar groups), despite the broader genetic base and larger pedigree distances in the former cultivar group. Second, levels of heterozygosity in modern NHB cultivars were not statistically different from those of SHB (χ2 = 4.0; df = 3; P > 0.05), despite the considerably higher levels of inbreeding in the former cultivar group (median F equal to 0.0035 and 0.0013, respectively). Furthermore, pedigree-based genetic distances were significantly correlated with Dsa (r = 0.57, P ≤ 0.0001), indicating that microsatellite markers are reliable tools in most cases to assess the genetic relationships among SHB cultivars. Finally, seven Vaccinium species constitute the current genetic base of cultivated SHB. In this article, we report on the expected genetic contributions of V. angustifolium Ait., V. constablaei Gray, V. corymbosum, V. darrowii Camp, V. elliottii Chapman, V. tenellum Ait., and V. virgatum Ait. (syn. V. ashei Reade) clones to 38 SHB cultivars.

scholarly journals Genetic Characterization of a Sheep Population in Oaxaca, Mexico: The Chocholteca Creole

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1172
Author(s):  
Teodulo Salinas-Rios ◽  
Jorge Hernández-Bautista ◽  
Araceli Mariscal-Méndez ◽  
Magaly Aquino-Cleto ◽  
Amparo Martínez-Martínez ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Characterization ◽  
Polymorphic Information Content ◽  
Isolated Population ◽  
Effective Number ◽  
Sheep Population ◽  
Blood Samples ◽  
Expected Heterozygosity

Creole sheep in México have undergone crossbreeding, provoking the loss of genetic variability. The objective of the present study is to determine the intra-racial genetic diversity, the genetic relationship with other genotypes, and the populational substructure of the Oaxacan Creole sheep. Twenty-nine blood samples were obtained of Creole sheep of the Oaxaca Mixteca region in México. A genetic analysis was made with 41 microsatellites recommended for studies of genetic diversity in sheep. An analysis was made of genetic diversity, populational structure, and genetic distance with 27 other sheep populations. The study found 205 alleles with a range of 2 to 9 by locus and an effective number of 3.33. The intra-racial analysis showed a moderate genetic diversity with values of expected heterozygosity of 0.686 and observed of 0.756, a mean polymorphic information content of 0.609, and a mean coefficient of consanguinity of −0.002. In interracial genetic diversity for the coefficients of consanguinity, the values were FIS = 0.0774, FIT = 0.16993, and FST = 0.10028, showing an elevated genetic distance with other creole breeds, but close to Argentine Creole, to another Creole of México and the Spanish Merino. Its genetic structure showed that it does not have any populational subdivision nor mixes with the others analyzed. It is concluded that it is a distinct and isolated population and is proposed as the creole breed “Chocholteca” for its conservation.

scholarly journals Molecular characterization of eight horse breeds in Algeria using microsatellite markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
MOHAMMED EL AMINE BENHAMADI ◽  
NAIMA BERBER ◽  
MOHAMMED BENYAROU ◽  
ABDELKADER AMEUR AMEUR ◽  
HADI YOUSSOUF HADDAM ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Molecular Characterization ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Significant Loss ◽  
Management Program ◽  
Factorial Correspondence Analysis ◽  
Horse Population ◽  
Expected Heterozygosity

 Abstract. Amine BME, Naima B, Mohammed B, Abdelkader AA, Youssouf HH, Mohammed P, Suheill GSB. 2020. Molecular characterization of eight horse breeds in Algeria using microsatellite markers.  Biodiversitas 21: 4107-4115. The Algerian horse population Bar, Origin Barb unknown, Arabian-Barb, Arabian, Thoroughbred (Pur sang); French Trotter, Algerian saddle, and Saddle are under a significant loss of genetic variability. This should be evaluated before any breed conservation and management program. For this purpose, genetic analyzes of diversity and differentiation were carried out on eight breeds horses reared in Algeria with 17 microsatellites. All microsatellite markers were highly polymorphic in all Breeds. A total of 170 alleles from 17 microsatellite loci were detected in 279 horses. The average number of alleles per locus was highest in Arabian-Barb horses (7.88) and lowest in Saddle breed (4.94). The observed heterozygosity interbreeds ranged from 0.685 to 0.625 for Barb and Saddle, while the expected heterozygosity was ranged from 0.746 to 0.678 for Arabian-Barb and Algerian Saddle respectively, the Ho value is lower than that of  He value. Genetic diversity between breeds was significant, based on Fst. Three different approaches to assess genetic relationships have been applied; namely, genetic distances, factorial correspondence analysis, and admixture analysis have shown that a significant amount of genetic variation is maintained in populations of native horses and other breeds: the Barb, Arabian-Barb, Barb (Oi). Algerian Saddle and Saddle breeds appear to be the most genetically related and support that there are crosses between these two breeds.

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