scholarly journals Factors affecting Agrobacterium mediated transformation of indigenous Chlorella vulgaris Bayerinck

2018 ◽  
Vol 44 (2) ◽  
pp. 323-326
Author(s):  
Nadia Sharif ◽  
Neelma Munir ◽  
Faiza Saleem ◽  
Shugufta Naz
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Chlorella Vulgaris ◽  
Bacterial Density ◽  
Optimum Conditions ◽  
Health Food ◽  
Feed Supplement ◽  
Factors Affecting ◽  
Agrobacterium Mediated Transformation

Chlorella vulgarisis Bayerinck widely used as a health food, feed supplement, as well as in the pharmaceutical, biofuel and cosmetics industries. It has been used to determine optimum transformation conditions through Agrobacterium tumefaciens. It has been revealed that bacterial density of OD600 = 1.0, 3 days of co-cultivation at 25°C in pH 5.5, and 100 μM acetosyringone are the optimum conditions to transform C. vulgaris.

scholarly journals Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

2011 ◽  
Vol 49 (No. 6) ◽  
pp. 255-260 ◽  
Author(s):  
J.I. Flores Solís ◽  
P. Mlejnek ◽  
K. Studená ◽  
S. Procházka
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transient Expression ◽  
Chenopodium Rubrum ◽  
Limiting Factors ◽  
Gus Gene ◽  
Efficient Tool ◽  
Factors Affecting ◽  
Agrobacterium Mediated Transformation ◽  
Glucuronidase Activity ◽  
Gene Coding

Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

scholarly journals Transcriptomic Analysis of Arabidopsis Seedlings in Response to an Agrobacterium-Mediated Transformation Process

2018 ◽  
Vol 31 (4) ◽  
pp. 445-459 ◽  
Author(s):  
Kaixuan Duan ◽  
Christopher J. Willig ◽  
Joann R. De Tar ◽  
William G. Spollen ◽  
Zhanyuan J. Zhang
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Defense Response ◽  
Time Course ◽  
Basic Research ◽  
Defense Responses ◽  
Transformation Process ◽  
Host Responses ◽  
Nucleic Acid Metabolism ◽  
Agrobacterium Strain ◽  
Agrobacterium Mediated Transformation

Agrobacterium tumefaciens is a plant pathogen that causes crown gall disease. This pathogen is capable of transferring the T-DNA from its Ti plasmid to the host cell and, then, integrating it into the host genome. To date, this genetic transformation ability has been harnessed as the dominant technology to produce genetically modified plants for both basic research and crop biotechnological applications. However, little is known about the interaction between Agrobacterium tumefaciens and host plants, especially the host responses to Agrobacterium infection and its associated factors. We employed RNA-seq to follow the time course of gene expression in Arabidopsis seedlings infected with either an avirulent or a virulent Agrobacterium strain. Gene Ontology analysis indicated many biological processes were involved in the Agrobacterium-mediated transformation process, including hormone signaling, defense response, cellular biosynthesis, and nucleic acid metabolism. RNAseq and quantitative reverse transcription-polymerase chain reaction results indicated that expression of genes involved in host plant growth and development were repressed but those involved in defense response were induced by Agrobacterium tumefaciens. Further analysis of the responses of transgenic Arabidopsis lines constitutively expressing either the VirE2 or VirE3 protein suggested Vir proteins act to enhance plant defense responses in addition to their known roles facilitating T-DNA transformation.

Preparation of Nanoscale CuO Powders

2010 ◽  
Vol 113-116 ◽  
pp. 1770-1773 ◽  
Author(s):  
Xi Hua Zhao ◽  
Min Xu
Keyword(s):  
Raw Materials ◽  
Precipitation Method ◽  
Optimum Conditions ◽  
Muffle Furnace ◽  
Factors Affecting ◽  
Direct Precipitation ◽  
Average Grain Size ◽  
Scherrer Formula ◽  
Average Size ◽  
Direct Precipitation Method

Cu(OH)2 precursor was synthesized by direct precipitation method and CuSO4 and NaOH were used as raw materials. Then, Cu(OH)2 precursor was calcined in muffle furnace at 400°C for 2h in order that CuO particle was obtained. Through the analysis of the factors affecting the CuO, the paper determined the optimum conditions for the synthesis of nano-CuO with the direct-precipitation method. Then the paper analyzed phase composition and crystal structure of samples using XRD and calculated the average grain size of samples by Scherrer formula, and observed and analyzed by TEM to characterize the morphology and particle size of samples. The optimum precipitation conditions are as follows: when the precipitation agent is 3.2:1, reaction time 40min and precipitant concentration 0.6mol.L-1. The average size of CuO particle prepared under the conditions is 18nm and the yield is 96%.

Consumer Purchasing Behaviour towards Organic Products in Thailand

2014 ◽  
pp. 411-433
Author(s):  
Yaowarat Sriwaranun ◽  
Christopher Gan ◽  
Minsoo Lee ◽  
David A. Cohen
Keyword(s):  
Income Group ◽  
High Income Group ◽  
Health Food ◽  
Food Stores ◽  
Factors Affecting ◽  
Highly Educated ◽  
Female Consumers ◽  
Organic Products ◽  
Purchasing Behaviour ◽  
Self Administered Questionnaire

This study investigates the factors affecting consumers’ decisions to purchase organic products. A self-administered questionnaire was used to collect the data from consumers who are primary shoppers for their households at five retail stores in the Bangkok metropolitan area. Using psychological and socio-demographics variables, exploratory factor analysis and logistic regression was used to examine consumers’ decisions to purchase organic products. Results suggest that consumers who are knowledgeable about organic products often purchase groceries at natural/health food stores, are concerned about health and food safety, and are more likely to purchase organic products. In addition, middle-aged female consumers who are highly educated and in the high income group are more likely to be organic consumers. In contrast, households who often dine out or consume takeaway food are less likely to purchase organic products.

Factors affecting Agrobacterium tumefaciens -mediated transformation of peppermint

2000 ◽  
Vol 19 (3) ◽  
pp. 304-310 ◽  
Author(s):  
X. Niu ◽  
X. Li ◽  
P. Veronese ◽  
R. A. Bressan ◽  
S. C. Weller ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Factors Affecting

scholarly journals Optimization of Insoluble and Soluble Fibres Extraction fromAgave americanaL. Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Amine Bouaziz ◽  
Manel Masmoudi ◽  
Amel Kamoun ◽  
Souhail Besbes
Keyword(s):  
Ionic Strength ◽  
Design Methodology ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Optimum Conditions ◽  
Box Behnken Design ◽  
Factors Affecting ◽  
Soluble Fibre ◽  
Experimental Design Methodology ◽  
Significant Factors

Experimental design methodology was used to determine significant factors affecting the extraction yield of soluble and insoluble fibres fromAgave americanaL. and in second time to find optimum conditions leading to the highest yield. Results clearly indicated that the temperature, the powder to water (P/W) ratio, and the agitation speed were the most important factors influencing fibres extraction yield which increased with temperature, P/W ratio, and agitation speed. Ionic strength affected significantly soluble fibre extraction yield and was the most important factor among nonsignificant ones influencing insoluble fibres extraction yield. Then, a Box-Behnken design was carried out to maximise fibres extraction. Selected optimal conditions were temperature: 90°C; P/W ratio: 0.1625; agitation speed: 400 rpm; and ionic strength: 1.5 g/L. These conditions yielded 93.02% and 80.46% of insoluble and soluble fibres, respectively. Concentrates showed high fibres purity and good functional properties.

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