scholarly journals Validity of Katex Test for Diagnosis of Visceral Leishmaniasis

2018 ◽  
Vol 4 (2) ◽  
pp. 45-47
Author(s):  
Ishrat Sharmin ◽  
AKM Quamruzzaman ◽  
Rezina Parveen ◽  
Rashida Akter Khanam ◽  
Md Abdullah Yusuf
Keyword(s):  
Bone Marrow ◽  
Urine Sample ◽  
Leishmania Donovani ◽  
Cross Sectional Study ◽  
Carbohydrate Antigen ◽  
Cross Sectional ◽  
Medical College ◽  
Kala Azar ◽  
Heat Stable ◽  
One Year

Background: Newly developed KAtex test can be used as a non invasive tool for diagnosis of Kala-azar.Objectives: The aim of the present study was to validate the KAtex method to diagnose VL.Methodology: This was a cross-sectional study carried out in the Department of Microbiology at Dhaka Medical College, Dhaka, Bangladesh in collaboration with the department of Parasitology at Institute of Epidemiology, Disease control and Research (IEDCR), Dhaka, Bangladesh for a period of one year. Clinically suspected Kala- azar (VL) cases of different age and sex attending IEDCR from different Kala-azar endemic areas of Bangladesh were selected for this study. Microscopy and culture was performed with Bone marrow (BM). KAtex was performed with urine sample. Urine samples taken from cases were pretreated to inactivate heat labile materials which might cause a false positive reaction. Antigen which is detected by KAtex is heat stable carbohydrate antigen. Latex sensitized with antibodies raised against Leishmania donovani antigen was mixed with the urine sample on a glass slide. No agglutination indicates absence of antigen in urine.Result: Cases were 130. Among 130 clinically suspected VL cases, 70 (53.85%) cases were BM positive and 60 (46.15%) cases were BM negative. All the 70 BM positive cases were positive by KAtex. Among 60 BM negative cases, 15 were positive by KAtex. The sensitivity of KAtex is 100% and specificity is 75%. Highest percentage (52.86%) of bone marrow positive cases were below 10 years of age group.Conclusion: In conclusion, KAtex test is a good diagnostic tool for the detection of VL.Bangladesh Journal of Infectious Diseases, December 2017;4(2):45-47

scholarly journals Comparison of KAtex, Bone Marrow Aspiration and DAT for the Diagnosis of Visceral Leishmaniasis

2019 ◽  
Vol 6 (1) ◽  
pp. 12-15
Author(s):  
Ishrat Sharmin ◽  
AKM Quamruzzaman ◽  
Rezina Parveen ◽  
M Abdulah Yusuf ◽  
Rashida Akter Khanam
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Bone Marrow Aspiration ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Medical College ◽  
Kala Azar ◽  
History Of ◽  
One Year

Background: Newly developed KAtex test can be used as a non invasive tool for diagnosis of Kala-azar. Objectives: The aim of the present study was to compare KAtex, Bone marrow aspiration and DAT to diagnose VL. Methodology: This cross-sectional study was carried out in the Department of Microbiology at Dhaka Medical College, Dhaka, Bangladesh in collaboration with the Department of Parasitology, Institute of Epidemiology, Disease Control and Research (IEDCR), Dhaka, Bangladesh for a period of one year. Clinically suspected Kala-azar (VL) cases of different age and sex attending IEDCR, Dhaka from different Kala-azar endemic areas of Bangladesh were selected for this study. Patients having fever for more than 2 weeks, with or without splenomegaly, having history of loss of body weight following onset of fever were clinically suspected as Kala-azar cases. Microscopy and culture was performed in bone marrow (BM). KAtex was performed with urine sample. Agglutination of sensitized latex indicated presence of Leishmania donovani antigen in urine and thereby visceral leishmaniasis. No agglutination indicates absence of antigen in urine. DAT was done with serums of all cases. Result: Among 130 clinically suspected VL cases, 70 (53.85%) cases were BM positive and 60(46.15%) cases were BM negative. All the 70 BM positive cases were positive by KAtex and DAT. Among 60 BM negative cases, 15 were positive by KAtex and 23 were positive by DAT. The sensitivity of KAtex was 100.0% and specificity was 75.0%. The sensitivity of DAT was 100.0% and specificity is 61.6%. Conclusion: In conclusion, KAtex test is a good diagnostic tool for the detection of VL in comparison with DAT. Bangladesh Journal of Infectious Diseases, June 2019;6(1):12-15

scholarly journals Validity of KAtex Test for the Diagnosis of Visceral leishmaniasis in Endemic Region of Bangladesh

2015 ◽  
Vol 1 (1) ◽  
pp. 8-11
Author(s):  
Zakir Hossain Habib ◽  
Afzalunnessa Binte Lutfor ◽  
Sanya Tahmina Jhora ◽  
Imtiaz Ahmed ◽  
Hasina Akhter
Keyword(s):  
High Sensitivity ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Endemic Region ◽  
Cross Sectional ◽  
Medical College ◽  
Kala Azar ◽  
Blood And Urine Samples ◽  
One Year ◽  
Age And Sex

Background: Early diagnosis of Kala-azar is of prime importance to reduce the transmission of the disease. Objective: The purpose of the present study was to evaluate KAtex test for the diagnosis of Kala-azar. Methodology: This cross sectional study was carried out in Department of Microbiology at Sir Salimullah Medical College, Dhaka for a period of one year from July 2007 to June 2008. Patients were selected clinically form all age and sex group hailing from endemic region of Mymensingh in a semi purposive manner excluding those who have already started taking medicine or refused to participate in the study.  Blood and urine samples were collected from the patients admitted in Sir Salimullah Medical College, Dhaka, Mymensingh Medical College and Bhaluka Health Complex, Mymensingh.  Result: Among 150 of the clinically selected patients, total 100 cases were confirmed to be positive for Kala-azar by rK39 ICT test. Age and sex matched 25 healthy persons each from endemic and non-endemic area was taken as control. Among the 100 sero-confirmed cases KAtex was positive in 87 (sensitivity of 87%). Among the 50 controls none were positive by KAtex test resulting in specificity of these tests 100% and 98% respectively. Conclusion: KAtex test has high sensitivity and specificity for the diagnosis of Kala-azar patients at endemic region of Bangladesh.Bangladesh J Infect Dis 2014;1(1):8-11

scholarly journals Screening of Thyroid Function in the First Half of Pregnancy

2016 ◽  
Vol 29 (1) ◽  
pp. 26-31
Author(s):  
Shaila Naznine Tania ◽  
Ferdousi Islam
Keyword(s):  
Pregnant Women ◽  
Thyroid Function ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Thyroid Disorder ◽  
Eligibility Criteria ◽  
Cross Sectional ◽  
Medical College ◽  
One Year ◽  
Serum Tsh

Objective: The study was conducted to find out any alterations in thyroid function status in first half of pregnancy (up to 20 weeks), with ultimate aim of deciding the usefulness of routine screening of thyroid function in pregnancy.Material and methods: A cross-sectional study was conducted in the department of Obstetrics & Gynaecology, Dhaka Medical College and Hospital, Dhaka, Bangladesh, over a period of one year from July 2011 to June 2012 on pregnant women in their 1st half of pregnancy to screen for the thyroid function. Based on predefined eligibility criteria, a total of 230 pregnant women were purposively included in the study. Thyroid function status was assessed by measuring serum levels of TSH, free thyroxine (FT4), and free tri-iodothyronine (FT3). Women with thyroid disorders were excluded.Results: The mean age of the patients being 24 years. Over 90% of the women were housewife and majority (88.7%) was educated. Over one-quarter of women was overweight with mean body mass index being 22.1 ± 4.4 kg/m2. The women were predominantly multigravida with 56% in 1st trimester 44% in the 1st half of 2nd trimester of pregnancy (13-20 weeks). Based on trimester’s specific range of serum TSH in the 1st and 1st half of 2nd trimester (13-20 weeks) of pregnancy,13% of the patients were hypothyroid and 3% were hyperthyroid thus yielding a total of 37(16%) pregnant women with abnormal thyroid function status.Conclusion: The study concluded that one in every six women may have thyroid disorder in the first half of pregnancy and subclinical hypothyroidism is four times more common than the subclinical hyperthyroidism.Bangladesh J Obstet Gynaecol, 2014; Vol. 29(1) : 26-31

scholarly journals Evolution of diarrheagenic Escherichia coli pathotypes in India

2019 ◽  
Vol 11 (04) ◽  
pp. 346-351
Author(s):  
Pankaj Singh ◽  
Sharda C. Metgud ◽  
Subarna Roy ◽  
Shashank Purwar
Keyword(s):  
Escherichia Coli ◽  
Cross Sectional Study ◽  
Clinical Settings ◽  
Accurate Identification ◽  
Cross Sectional ◽  
E Coli ◽  
Medical College ◽  
Diarrheagenic Escherichia Coli ◽  
Proper Management ◽  
Heat Stable

Abstract CONTEXT: Diarrheagenic Escherichia coli (DEC) is the leading cause of infectious diarrhea in developing countries. On the basis of virulence and phenotypic characteristics, the DEC is categorized into multiple pathotypes. Each pathotype has different pathogenesis and geographical distribution. Thus, the proper management of disease relies on rapid and accurate identification of DEC pathotypes. AIMS: The aim of the study was to determine the prevalence of DEC pathotypes in India. MATERIALS AND METHODS: A cross-sectional study was carried out between January 2008 and December 2012 at Jawaharlal Nehru Medical College and KLES Dr. Prabhakar Kore Hospital and Medical Research Center, Belgaum (Karnataka), India. A total of 300 stool samples were collected from diarrhea patients with age >3 months. The DEC was identified by both conventional and molecular methods. RESULTS: Of 300 samples, E. coli were detected in 198 (66%) and 170 (56.6%) samples by culture and polymerase chain reaction, respectively. Among DEC (n = 198) isolates, eae gene (59.5%) was the most prevalent followed by stx (27.7%), east (27.2%), elt (12.6%), est (10.6%), ipaH (5.5%), and eagg (1.5%) genes. On the basis of virulence genes, enteropathogenic E. coli (33.8%) was the most common pathotype followed by Shiga toxin-producing E. coli (STEC, 23.2%), enterotoxigenic E. coli (ETEC, 13.6%), enteroinvasive E. coli (5.5%), enteroaggregative heat-stable enterotoxin 1-harboring E. coli (EAST1EC, 4.5%), STEC/ETEC (3.5%), STEC/enteroaggregative E. coli (STEC/EAEC, 1.0%), and EAEC (0.05%). CONCLUSIONS: The hybrid DEC is potentially more virulent than basic pathotypes. The pathotyping should be included in clinical settings for the proper management of DEC-associated diarrhea.

scholarly journals Leucocytosis in Febrile Seizure

2011 ◽  
Vol 31 (3) ◽  
pp. 188-191
Author(s):  
AR Ojha ◽  
UR Aryal
Keyword(s):  
Febrile Seizure ◽  
Leucocyte Count ◽  
Cross Sectional Study ◽  
Differential Count ◽  
Total Leucocyte Count ◽  
High Count ◽  
Cross Sectional ◽  
Peripheral Blood Leucocyte ◽  
Medical College ◽  
One Year

Introduction: Febrile seizure (FS) is a common condition affecting 2-5% of children. Peripheral blood leucocyte count with its differential is an initial test looking for the cause of fever and high count is usually accounted for the seizure activity. Objectives: To find the incidence of febrile seizure and to evaluate the relationship between fever duration before seizure, seizure duration and the total leucocyte as well as neutrophil response. Methodology: It is a cross sectional study done at Kathmandu Medical College and Teaching Hospital, Kathmandu, Nepal, for one year. After an informed consent, children from 6 months to 6 years with FS were admitted. Those with afebrile seizures and who refused to give consent were excluded. A detail history and examination was done on admission. All were investigated for the source of fever including total and differential count. Results: Out of a total 1742 children admitted, 115 (6.6%) children had febrile seizure. Simple febrile seizure was observed in 93(81%) and 22(19%) was complex febrile seizure. 42% of them had leucocytosis. The duration of fever before the onset of seizure is found to be negatively correlated with total leucocyte count (r = -0.418, p<0.001) and neutrophils count alone (r = -0.375, p<0.001). The duration of seizure is not correlated to both the total leucocyte count (r = -0.162, p = 0.85) and the neutrophil (r = -0.109, p= 0.247). Conclusion: The incidence of febrile seizure is 6.6%. Leucocytosis and neutrophilia in children is negatively correlated with the duration fever before the onset of seizure, associated with underlying infection if any and is not related to seizure event and its duration. Thus any child with febrile seizure with high leucocyte count should be evaluated for infection. Key words: Fever; Leucocytosis; Neutrophilia; Seizure DOI: http://dx.doi.org/10.3126/jnps.v31i3.4577 J Nep Paedtr Soc 2011;31(3):188-191   

scholarly journals Study to Determine the Incidence of High HbA1c in Patients with Ischemic Stroke at Bilawal Medical College, Kotri, Sindh, Pakistan

2021 ◽  
pp. 20-24
Author(s):  
Atif Ahmed ◽  
Kuldeep Poorani ◽  
Mohammad Amjad Kalhoro ◽  
Nisar Ahmed Khokar ◽  
Abdul Ghaffar Dars
Keyword(s):  
Ischemic Stroke ◽  
Blood Glucose ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
College Hospital ◽  
Cross Sectional ◽  
Ischemic Disease ◽  
Medical College ◽  
Cerebral Ischemic ◽  
One Year

Aim: To study the incidence of HbA1c elevation in patients with ischemic stroke whose blood glucose levels were normal within the last six months. Study Design: A cross-sectional study. Place and Duration: This cross-sectional study of the 96 patients with cerebral ischemic disease selected from the internal medicine department of Bilawal medical college Hospital Kotri, Sindh, Pakistan for one-year duration from August 2019 to August 2020. Methods: Crossectional study was conducted in the internal medicinal department of Bilawal Medical college Hospital, Kotri Sindh, Pakistan for the period of one year from August 2019 to August 2020. Total 96 study subjects were selected with cerebral ischemic disease, 62 were males and 34 were females. Many factors such as age more than 20 years, ischemic stroke and RBG (Random Blood Glucose) were also evaluated from each participant. SPSS 23.00 version was used for statistical analysis of research data. Results: 57.01 ± 13.4 years was the mean age of the patients. 62 (64.6%) patients were male and 34 (35.4%) were female. The male to female ratio was 2:1. HbA1c was elevated in 35 (36.5%) of 96 patients, while 61 (63.5%) had normal HbA1c.  The distribution of HbA1c elevation by gender showed that 20 (57.1%) patients with elevated HbA1c were men and 15 (42.9%) were women. Forty-three (70.5%) of 61 patients with normal HbA1c were male and 18 (29.5%) were female. Conclusion: it was concluded that glycated haemoglobin plays an important role for reducing the macro complication of Diabetes and the major macro complication observed was cerebral ischemic stroke among the people with elevated HbA1c.

scholarly journals Epidemiological study of animal bite victims and admission in general surgery department, in Southern Odisha: a cross sectional institutional study

2017 ◽  
Vol 4 (10) ◽  
pp. 3470 ◽  
Author(s):  
Sibaprashad Pattanayak ◽  
Tapan Kumar Malla ◽  
Bipin Kishore Bara ◽  
Manoj Kumar Behera
Keyword(s):  
General Surgery ◽  
Cross Sectional Study ◽  
Human Rabies ◽  
Cross Sectional ◽  
Medicine Department ◽  
Medical College ◽  
Recent Estimate ◽  
Animal Bites ◽  
One Year ◽  
Surgery Department

Background: Human rabies continues to be endemic in India and according to recent estimate 20,000 persons die of this disease every year. Hence this study was conducted to know the prevalence and pattern of animal bites during last one year and to determine the rate of admission in Department of Surgery.Methods: This study was conducted in the casualty, Department of Community Medicine, Department of Surgery at M. K. C. G. Medical College, Odisha, India between 1st April 2016 - March 31st, 2017. It was a cross sectional study, where convenient sampling method was used. The sample size was 6242 subjects, above 1year age group selected randomly within last 1 year. The socio demographic characteristics, epidemiological and rate of admission in surgery department were studied.Results: It was found that during last one year, out of 6242 animal bite cases, majority of them were bitten by dogs 4785 (76.66%). It was found that 5617 (90%) are category III bite, 548 (8.78%) are category II bites, 77 (1.23%) are category I bite. Parts of body bitten by animals were limbs 5828 (93.37%), face 312 (5%), back 52 (0.83%). Out of 6242 cases, 348 (5.5%) cases were admitted in General Surgery Department.Conclusions: The prevalence of dog bite was found to be more and was of category III in nature and part of body affected were mostly limbs. Prevalence of bite was nearly equal among male and female. Around 5% of the total animal bite cases required admission in Department of Surgery.  

scholarly journals A-V Fistula Blood Flow and Complications: A One Year Survey

2014 ◽  
Vol 15 (2) ◽  
pp. 110-113
Author(s):  
Tania Mahbub ◽  
Md. Nizamuddin Chowdhury ◽  
Ferdous Jahan ◽  
Aparna Das ◽  
Md. Motlabur Rahman ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cross Sectional Study ◽  
Visualization Technique ◽  
Colour Doppler ◽  
College Hospital ◽  
Cross Sectional ◽  
Medical College ◽  
Study Population ◽  
One Year ◽  
Venous Fistula

Background: Worldwide haemodialysis(HD) is the mostly used method of renal replacement therapy. Arteriovenous fistula use is on rise due to Fistula First Initiative due to least complications of fistulae than catheters.But they are also subjected to many complications. Thereby appropriate surveillance of the fistulae is important.Objectives: To measure the blood flow as well as fistula related complications in study population. Materials and Methods: This was a cross sectional study, conducted in the department of Nephrology, Dhaka Medical College Hospital during September 2010 to December 2011.Total 118 subjects were included in the study. Besides visualization technique colour Doppler ultrasonogram of fistula and  echocardiography were performed for each patients. Results: Most of the patients had adequate fistula flow. Aneurysm was most common complication. Many subjects were suffered from primary and secondary fistula failure. Conclusion: Arterio-Venous fistula is subjected to many complications and appropriate surveillance should be launched to detect complications and to prevent fistula failure.DOI: http://dx.doi.org/10.3329/jom.v15i2.20681 J MEDICINE 2014; 15 : 110-113

scholarly journals A community based study of scenario of glaucoma in Aligarh, India

2019 ◽  
Vol 6 (9) ◽  
pp. 4098
Author(s):  
Syed Zulfequar Ahmad ◽  
Anees Ahmad ◽  
Najam Khalique ◽  
Yasir Alvi
Keyword(s):  
Open Angle Glaucoma ◽  
Cross Sectional Study ◽  
Applanation Tonometry ◽  
Angle Closure ◽  
Cross Sectional ◽  
Medical College ◽  
Primary Angle Closure Glaucoma ◽  
Study Population ◽  
Eye Examination ◽  
One Year

Background: Glaucoma is the leading cause of irreversible blindness worldwide and is second only to cataracts as the most common cause of blindness overall. In the developing world, the prevalence of glaucoma is expected to rise even more dramatically as the population of adults has doubled within a span of few decades. So this study was done with an objective of finding out the prevalence of glaucoma among adults aged 40 years and above in the field practice area of the Department of Community Medicine, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh.Methods: A cross-sectional study was carried out for the duration of one year. A total of 680 persons were interviewed and examined by a trained ophthalmologist for detailed eye examination including visual acuity, refraction and slit-lamp bio-microscopy, intraocular pressure (IOP) by Keeler Pulsair non-contact tonometer confirm by applanation tonometry, gonioscopy, and dilated fundus examination after ruling out the risk of angle closure. Anderson criteria were used to diagnose glaucomatous visual field defect.Results: In our study population, the IOP of the majority of population lied in 11-15 mm Hg with a mean IOP of 13.42±4.09 mmHg. In regard to optic disc examination, a cup to disc ratio of >0.6 was found in 35 eyes. According to the predefined criteria, a total of 31 subjects were diagnosed as glaucoma in either one or both eyes. The overall prevalence of glaucoma was found to be 4.6% (31/680), with the prevalence of primary open angle glaucoma, primary angle closure glaucoma, normotensive glaucoma and secondary glaucoma as 1.3%, 1.2%, 1.2% and 0.9% respectively.Conclusions: The prevalence of glaucoma is quite high in an elder population of Aligarh. This should warrant more intensive activities, focusing not only on early diagnosis and management of glaucoma but also include strengthening preventive ophthalmic care.

scholarly journals Significance of Reporting Cannon Balls in Diagnosing Bacterial Vaginosis Infection in PAP Smear

2021 ◽  
Vol 23 (2) ◽  
pp. 109-113
Author(s):  
Kricha Pande ◽  
Shraddha Koirala ◽  
Astha Shrestha
Keyword(s):  
Bacterial Vaginosis ◽  
Pap Smear ◽  
College Teaching ◽  
Cross Sectional Study ◽  
Gardnerella Vaginalis ◽  
Pap Smears ◽  
Cross Sectional ◽  
Medical College ◽  
The Mean ◽  
One Year

Bacterial vaginosis is the most common cause of vaginitis. It is caused by depopulation of lactobacilli from the normal vaginal flora and overgrowth of Gardnerella vaginalis and other anaerobic species. Pap smear being a screening and diagnostic tool for malignancy, is equally important to diagnose infective pathology as well. This study tried to establish the significance of reporting the presence of cannon balls in Pap smears and whether their presence suggested bacterial vaginosis or not. This was a cross sectional study conducted at Nepal Medical College, Teaching Hospital (NMCTH), Kathmandu for duration of one year (January 2020 to December 2020). Pap smears were sent from gynaecology department and standard routine Pap stain was done in Pathology Department. Presence of bacterial vaginosis and cannon balls were noted. Total of 799 Pap smears were received. The mean age of bacterial vaginosis was 38.2 ± 9.6 years. The age group of 31-40 years (n=32, 39%) was most commonly affected. Bacterial vaginosis was seen in 81 pap smears out of 799 pap smears. The prevalence of bacterial vaginosis was 10.1%. Of the total of 799 cases of Pap smears, cannon balls were seen in 9.1% cases and among the bacterial vaginosis, cannon balls were seen in 48% cases. There was significant association between cannonballs and bacterial vaginosis (p < 0.0001). Thus, the present study re-enforces the fact that Pap smear is also very effective tool to diagnose bacterial vaginosis.

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