scholarly journals Isolation of Keratinophilic Fungi from Soil in Khairpur City, Sindh, Pakistan

1970 ◽  
Vol 24 (1) ◽  
pp. 79-80 ◽  
Author(s):  
Irshad Hussain Soomro ◽  
Yasmeen Faiz Kazi ◽  
Miandad Zardari ◽  
Abdul Hussain Shar

One hundred and twenty five soil samples were collected from five areas in the vicinity of Khairpur city, Sindh, Pakistan and screened for the presence of keratinophilic fungi by using hair bait technique. A total 253 isolates belonged to eight genera and eleven species of keratinophilic fungi were recovered. Maximum number (153/253, 60.5%) of keratinophilic fungi was recovered from farm land and poultry soils. Aspergillus niger was most prevalent species and represented 20.2% of the total number isolated. A. flavus and A. fumigatus were almost equal in their prevalence and represented 11.9 and 11.1%, respectively. Botrytis cinaria comes next constituting 9.9%; followed by Mucor spp. (9.1%) and Chochliobolus lunatus (8.3%). Other species isolated less frequently included Chrysosporum asperatum (5.9%), Fusarium spp. (5.1%), Penicillium spp. (3.1%) and A. wentii (2.4%). The distribution pattern of the different keratinophilic fungi and their significance are discussed.Keywords: Keratinophilic fungi, Prevalence, Keratinase, Hair bait techniqueDOI: http://dx.doi.org/10.3329/bjm.v24i1.1246 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 79

Author(s):  
Janet Olufunmilayo Williams ◽  
Nosayame Thomas Osahon

The potential of fungi as bio degraders of micro plastic particles was assessed using standard microbiological and Fourier transformed Infra-Red (FTIR) spectroscopic analysis methods. The highest mean Total Heterotrophic fungal (THF) count of 4.24x104 cfu/ml was obtained with the least THF (2.72x104 cfu/ml) recorded during the dry season. Mean hydrocarbon Utilizing Fungal (HUF) count was highest (1.78x104 cfu/ml) during the wet period while the least HUF count (1.46x104 cfu/ml) was recorded during the dry period. Spectra of FTIR showed that the water contained microplastic particles in these proportions; polyethylene of low density (LDPE) 0.01%, 0.11% protein, 0.15% polystyrene, 0.37% polyamide, 1.14% cellulose, 1.21% polyurethane, 1.90% polyvinyl chloride, 3.11% polyester and 92% polypropylene, respectively. Species of fungi identified were Aspergillus niger, Penicillium spp., Rhizopus spp., Mucor spp., Aspergillus nidulans, Fusarium spp., Microsporum canis, and Aspergillus fumigatus. Among the fungal isolates, A. niger and A. fumigatus were most active in degrading the micro plastic (polypropylene) with mean % weight loss of 71.09% and 53.09%, respectively while the least active was Penicillium spp. with a mean % weight loss of 28.64% during the study period. The order of degradation was Aspergillus niger > A. fumigatus > A. nidulans > Fusarium spp. > Rhizopus spp. > Microsporum canis > Mucor spp. > Penicillium spp. The potential to degrade micro plastic particles by these fungi can be harnessed. The foremost active fungi degrading potentials are as follows: A. niger (71.1%) and A. fumigatus (53.1%). The study has shown that isolates of fungi from Ohiakwu River in Nigeria possess the ability to degrade micro plastic (polypropylene) particles. Therefore, this research is of tremendous importance for industrial development and additionally for future research purposes.


2016 ◽  
Vol 13 (1) ◽  
pp. 79-86
Author(s):  
MM Rahman ◽  
MS Islam ◽  
MZ Alam ◽  
M Ashrafuzzaman

The Experiments were carried out during the period of July 2001 to December 2002. Infestation of poultry feeds by fungi and aflatoxins content in feeds were determined. Ten feed samples were collected from ten different farms located in three different areas of Bangladesh. Five different fungal species belonging four genera were detected which were Aspergillus flavus, Aspergillus niger, Fusarium spp, Rhizopus stolonifer and Penicillium spp. Among them Aspergillus flavus and Aspergillus niger were found in all the feed samples and the rest were occasionally found. Effect of storage and moisture content of feeds were studied for determining the influence on occurrence of fungal flora in feeds. Higher the storing period of feed along with higher moisture content resulted superior incidences of fungal population in stored feeds. Studied on storage practice with 4 duration of F1 sample revealed that the presence of aflatoxins were 30.52ppb, 33.36ppb, 37.32ppb and 48.35ppb of 5, 10, 15 and 20 days after production of feeds, respectively.J. Bangladesh Agril. Univ. 13(1): 79-86, June 2015


2017 ◽  
Vol 2 (3) ◽  
pp. 129
Author(s):  
Aurilio Garcia ◽  
Jobson Bortot ◽  
Marco Antônio Ferraz ◽  
Lívia Mota ◽  
Tassius Borsatto ◽  
...  

Os autores avaliaram o perfil da microbiota conjuntival fúngica de estudantes da área das Ciências da Saúde do Centro Universitário de Volta Redonda – UniFOA. Foram estudados 200 acadêmicos, sendo 100 pertencentes ao ciclo básico, com restrição ao ambiente hospitalar, e 100 do ciclo clínico, com efetivo contato. O material foi coletado do saco conjuntival e semeado em meio de cultura Sabouraud-dextrose-agar, e mantido em temperatura ambiente.As culturas foram positivas em 26 alunos, correspondendo a 13% do total examinado. Foram isolados os seguintes fungos: 7 (26,92%) Candida albicans, 5 (19,23%) Rhodotorula spp., 6 (23,07%) Penicillium spp., 3 (11,53%) Aspergillus niger, 3 (11,53%) Fusarium spp., e 2 (7,69%) Aspergillus fumigatus. Este resultado é compatível com a maioria dos estudos anteriores realizados por outros autores.


2017 ◽  
Vol 2 (3) ◽  
pp. 129-132
Author(s):  
Aurilio Garcia ◽  
Jobson Bortot ◽  
Marco Antônio Ferraz ◽  
Lívia Mota ◽  
Tassius Borsatto ◽  
...  

Os autores avaliaram o perfil da microbiota conjuntival fúngica de estudantes da área das Ciências da Saúde do Centro Universitário de Volta Redonda – UniFOA. Foram estudados 200 acadêmicos, sendo 100 pertencentes ao ciclo básico, com restrição ao ambiente hospitalar, e 100 do ciclo clínico, com efetivo contato. O material foi coletado do saco conjuntival e semeado em meio de cultura Sabouraud-dextrose-agar, e mantido em temperatura ambiente.As culturas foram positivas em 26 alunos, correspondendo a 13% do total examinado. Foram isolados os seguintes fungos: 7 (26,92%) Candida albicans, 5 (19,23%) Rhodotorula spp., 6 (23,07%) Penicillium spp., 3 (11,53%) Aspergillus niger, 3 (11,53%) Fusarium spp., e 2 (7,69%) Aspergillus fumigatus. Este resultado é compatível com a maioria dos estudos anteriores realizados por outros autores.


2008 ◽  
Vol 1 ◽  
pp. MBI.S943 ◽  
Author(s):  
V.H. Tournas ◽  
E.J. Katsoudas

Various herbal teas including German chamomile, Chrysanthemum Vascuflow herb tea, hop, jasmine and orange flowers, sweet marjoram, spearmint and thyme leaves, and papaya-mint tea as well as coffee substitutes (Bambu instant Swiss, Teeccino chocolate-mint, and Teeccino Mediterranean Espresso) were analyzed for fungal contamination and the presence of aerobic mesophilic bacteria (APC). The results of this investigation showed that fungal counts reached levels as high as 5.8 × 105 colony forming units (cfu) per gram. German chamomile harbored the highest fungal contamination. The most common fungi found in herbal teas were Aspergillus niger, Penicillium spp., Eurotium rubrum, E. chevalieri, A. flavus, Fusarium spp., Alternaria alternata, and yeasts. Among the coffee substitutes, only the chocolate-mint coffee was contaminated with low numbers (<1.0 × 103 cfu g-1) of E. rubrum, Ulocladium spp. and Phoma spp., and with yeasts (< 100–6.8 × 103 cfu g-1). Aerobic mesophilic bacteria were recovered from 100% of the herbal tea, chocolate-mint and Mediterranean Espresso, and from 50% of the Bambu instant Swiss coffee samples. The highest APC counts of 1.2 × 107 cfu g-1 were observed in spearmint leaves.


2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Mehr -un-Nisa ◽  
Mussadiq Hussain ◽  
Basharat Mehmood ◽  
Muhammad Tariq-Khan ◽  
Hafiz Matee Rehman

The current study was carried out on the prevalence of mycoflora associated with maize seeds using blotter paper method. Maize seed samples were collected from six different locations of district Poonch, Azad Jammu and Kashmir. Objective of this study was to determine the fungi associated with maize seeds. A total of seven species of fungi were isolated. Penicillium spp., Fusarium spp., Aspergillus niger, A. flavus, Fusarium spp., Fusarium oxysporum and Pythium spp., were identified. Davigali had the infection percentage of 72 % in all locations. Occurrence frequency and type of fungi isolated varied with location. Prevalence of pathogenic fungi with maize seeds of district Poonch was found variable. Resistant varieties of maize, maintaining temperature, relative humidity and their treatment is suggested to reduce disease and increase yield.


2013 ◽  
Vol 13 (2) ◽  
pp. 79-86
Author(s):  
Sadhana Sah ◽  
Ranjita Shrestha ◽  
Sadikchya Koirala ◽  
Keshab Bhattarai

Crude petroleum ether and methanol extracts of different parts of five medicinal plants collected from Terai region, namely, Asparagus racemosus, Catharanthus roseus, Hedychium coronarium, Mimosa pudica and Terminalia chebula were examined for their antimicrobial activity against different pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Klebsella spp., Salmonella paratyphi, Bacillus subtilis, Aspergillus niger, Fusarium spp. and Penicillium spp. From the obtained growth curves of methanol extract of Terminalia chebula and petroleum ether and methanol extracts of Hedychium coronarium, it can be said these extracts have relatively high bacteriostatic property than other plant extracts. Similarly, the zone of inhibitions observed during antifungal assay by methanol extract of Terminalia chebula against Aspergillus niger, Fusarium spp. and Penicillium spp. are comparable to that of Itraconazole (antifungal drug). It shows that Terminalia chebula has fungistatic property. Phytochemical screening revealed the presence of alkaloids, coumarins, flavonoids, terpenoids, steroids and glycosides. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 79-86 DOI: http://dx.doi.org/10.3126/njst.v13i2.7718


1969 ◽  
Vol 88 (1-2) ◽  
pp. 55-72
Author(s):  
Lorraine Vélez ◽  
Lydia I. Rivera ◽  
Rocío del P. Rodríguez ◽  
Irma Cabrera

A survey was conducted in experimental and commercial onion (Allium cepa L.) fields in the southern region of Puerto Rico to isolate and identify fungi associated with this crop. Fungi were isolated from air, soil, and tissue from leaves and bulbs that showed typical disease symptoms. Commercial seeds from three onion cultivars commonly used in Puerto Rico were examined. Overall, Aspergillus niger was the fungus most frequently isolated from soil, bulbs and seeds. Fusarium spp. and Penicillium spp. were frequently isolated from air; A. niger, Fusarium and Cladosporium, from soil; and A. niger, Penicillium and Sclerotium rolfsii, from bulbs. In onion foliage, Alternaria was the most common genus, followed by Stemphylium and Nigrospora. In commercial onion seeds, A. niger and Rhizopus in combination were the most frequent.


2017 ◽  
Vol 14 (1) ◽  
pp. 45
Author(s):  
Angela P. Cajiao
Keyword(s):  

El comercio del café es uno de los renglones económicos más importantes a nivel mundial, sin embargo, también es susceptible a contaminaciones desde su cosecha hasta su transformación. Para la realización de este estudio se recolectaron muestras de café cereza procedentes de diferentes municipios productores de Norte de Santander y posteriormente en el laboratorio se aislaron y caracterizaron fenotípicamente los siguientes agentes fúngicos del grano de café: Aspergillus  spp., Penicillium spp., Fusarium spp., Cladosporium  spp., Mucor spp. y  Rhizopus spp. con ayuda de claves taxonómicas. Los hongos que presentaron una alta incidencia fueron Aspergillus spp. y Fusarium spp. Entre las tres variables fisicoquímicas analizadas en el café cereza (pH, actividad de agua, % de humedad) se puede afirmar con certeza que la actividad de agua y el porcentaje de humedad influyen directamente en el número y tipo de aislamientos fúngicos obtenidos. 


Sign in / Sign up

Export Citation Format

Share Document