scholarly journals Mycofloral study of cultivated maize seed in district Poonch Azad Jammu and Kashmir

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Mehr -un-Nisa ◽  
Mussadiq Hussain ◽  
Basharat Mehmood ◽  
Muhammad Tariq-Khan ◽  
Hafiz Matee Rehman

The current study was carried out on the prevalence of mycoflora associated with maize seeds using blotter paper method. Maize seed samples were collected from six different locations of district Poonch, Azad Jammu and Kashmir. Objective of this study was to determine the fungi associated with maize seeds. A total of seven species of fungi were isolated. Penicillium spp., Fusarium spp., Aspergillus niger, A. flavus, Fusarium spp., Fusarium oxysporum and Pythium spp., were identified. Davigali had the infection percentage of 72 % in all locations. Occurrence frequency and type of fungi isolated varied with location. Prevalence of pathogenic fungi with maize seeds of district Poonch was found variable. Resistant varieties of maize, maintaining temperature, relative humidity and their treatment is suggested to reduce disease and increase yield.

2013 ◽  
Vol 2 (2) ◽  
pp. 97-101
Author(s):  
Muzammil Hussain ◽  
Muhammad U. Ghazanfar ◽  
Muhammad I. Hamid ◽  
Mubashar Raza

Seed borne mycoflora associated with ten commercial varieties of wheat viz. Blue silver, Faisalabad 85, Manthar-3, Pak 81, Parwaz 94, Pirsabaq 2005, Punjnad-1, Sariab-92, Sh-2002 and Wafaq-2001 was investigated through standard blotter paper and agar plate method by using Mann-Whitney U test. At least eleven fungal genera were recovered from seeds. The most frequently isolated fungi were Bipolaris sorokiniana (11.125%), Aspergillus flavus (9.825%), Alternaria alternata (7.15%) and Aspergillus niger (6.225%). It is apparent from the present investigation that all commercial wheat verities tested were contaminated by fungi. The rolled paper method was used to find out the effect of seed borne fungi on seed germination. Seeds of Pak 81, Wafaq-2001 and Blue silver were germinated in high proportion with variable number of normal and abnormal seedlings than the seeds of other varieties tested. The fungi associated with seeds of wheat cause dire diseases in wheat reducing the germination capacity.


Author(s):  
Janet Olufunmilayo Williams ◽  
Nosayame Thomas Osahon

The potential of fungi as bio degraders of micro plastic particles was assessed using standard microbiological and Fourier transformed Infra-Red (FTIR) spectroscopic analysis methods. The highest mean Total Heterotrophic fungal (THF) count of 4.24x104 cfu/ml was obtained with the least THF (2.72x104 cfu/ml) recorded during the dry season. Mean hydrocarbon Utilizing Fungal (HUF) count was highest (1.78x104 cfu/ml) during the wet period while the least HUF count (1.46x104 cfu/ml) was recorded during the dry period. Spectra of FTIR showed that the water contained microplastic particles in these proportions; polyethylene of low density (LDPE) 0.01%, 0.11% protein, 0.15% polystyrene, 0.37% polyamide, 1.14% cellulose, 1.21% polyurethane, 1.90% polyvinyl chloride, 3.11% polyester and 92% polypropylene, respectively. Species of fungi identified were Aspergillus niger, Penicillium spp., Rhizopus spp., Mucor spp., Aspergillus nidulans, Fusarium spp., Microsporum canis, and Aspergillus fumigatus. Among the fungal isolates, A. niger and A. fumigatus were most active in degrading the micro plastic (polypropylene) with mean % weight loss of 71.09% and 53.09%, respectively while the least active was Penicillium spp. with a mean % weight loss of 28.64% during the study period. The order of degradation was Aspergillus niger > A. fumigatus > A. nidulans > Fusarium spp. > Rhizopus spp. > Microsporum canis > Mucor spp. > Penicillium spp. The potential to degrade micro plastic particles by these fungi can be harnessed. The foremost active fungi degrading potentials are as follows: A. niger (71.1%) and A. fumigatus (53.1%). The study has shown that isolates of fungi from Ohiakwu River in Nigeria possess the ability to degrade micro plastic (polypropylene) particles. Therefore, this research is of tremendous importance for industrial development and additionally for future research purposes.


2020 ◽  
Vol 58 (7) ◽  
pp. 881-886
Author(s):  
Mayk Teles de Oliveira ◽  
Nathany Kelly Ribeiro Batista ◽  
Eric de Souza Gil ◽  
Maria do Rosário Rodrigues Silva ◽  
Carolina Rodrigues Costa ◽  
...  

Abstract The hospital environment requires special attention to air quality, since it needs to be healthy for the protection of patients and health professionals in order to prevent them against hospital infections. The objective of this study was to isolate, identify and evaluate the susceptibility profile of isolated fungi from two hospitals. For air sampling the impaction (Spin Air, IUL®) and passive sedimentation methods were used. For the isolation of fungi from surfaces, contact plates (RODAC®) were used. The identification of the fungi was performed by observing the macroscopic and microscopic aspects of the colonies, whereas for better visualization of fruiting structures, the microculture technique was performed on slides. To evaluate the susceptibility profile, the broth microdilution test recommended by CLSI was performed. Thirty-five isolates were identified: Aspergillus flavus (12), Aspergillus fumigatus (11), Aspergillus niger (1), Aspergillus terreus (2), Penicillium spp. (7), and Fusarium spp. (2) in the hospitals evaluated. All isolates had a minimum inhibitory concentration (MIC) more than 128 μg/ml for fluconazole; 0.5 to 4.0 μg/ml for amphotericin B (hospital 1), and all isolates from haospital 2 had MIC ≥2.0 μg/ml. In hospital 1, MIC for posaconazole ranged from 0.25 μg/ml to ≥32 μg/ml, and hospital 2 ranged from 0.5 to 1.0 μg/ml. The monitoring and evaluation of air quality and surfaces are essential measures for prevention and control of hospital infections, as these microorganisms are becoming increasingly resistant to antimicrobial agents, thus making treatment difficult, especially in immunocompromised individuals.


2016 ◽  
Vol 13 (1) ◽  
pp. 79-86
Author(s):  
MM Rahman ◽  
MS Islam ◽  
MZ Alam ◽  
M Ashrafuzzaman

The Experiments were carried out during the period of July 2001 to December 2002. Infestation of poultry feeds by fungi and aflatoxins content in feeds were determined. Ten feed samples were collected from ten different farms located in three different areas of Bangladesh. Five different fungal species belonging four genera were detected which were Aspergillus flavus, Aspergillus niger, Fusarium spp, Rhizopus stolonifer and Penicillium spp. Among them Aspergillus flavus and Aspergillus niger were found in all the feed samples and the rest were occasionally found. Effect of storage and moisture content of feeds were studied for determining the influence on occurrence of fungal flora in feeds. Higher the storing period of feed along with higher moisture content resulted superior incidences of fungal population in stored feeds. Studied on storage practice with 4 duration of F1 sample revealed that the presence of aflatoxins were 30.52ppb, 33.36ppb, 37.32ppb and 48.35ppb of 5, 10, 15 and 20 days after production of feeds, respectively.J. Bangladesh Agril. Univ. 13(1): 79-86, June 2015


2017 ◽  
Vol 2 (3) ◽  
pp. 129
Author(s):  
Aurilio Garcia ◽  
Jobson Bortot ◽  
Marco Antônio Ferraz ◽  
Lívia Mota ◽  
Tassius Borsatto ◽  
...  

Os autores avaliaram o perfil da microbiota conjuntival fúngica de estudantes da área das Ciências da Saúde do Centro Universitário de Volta Redonda – UniFOA. Foram estudados 200 acadêmicos, sendo 100 pertencentes ao ciclo básico, com restrição ao ambiente hospitalar, e 100 do ciclo clínico, com efetivo contato. O material foi coletado do saco conjuntival e semeado em meio de cultura Sabouraud-dextrose-agar, e mantido em temperatura ambiente.As culturas foram positivas em 26 alunos, correspondendo a 13% do total examinado. Foram isolados os seguintes fungos: 7 (26,92%) Candida albicans, 5 (19,23%) Rhodotorula spp., 6 (23,07%) Penicillium spp., 3 (11,53%) Aspergillus niger, 3 (11,53%) Fusarium spp., e 2 (7,69%) Aspergillus fumigatus. Este resultado é compatível com a maioria dos estudos anteriores realizados por outros autores.


2018 ◽  
pp. 1-7
Author(s):  
Elias Nortaa Kunedeb Sowley ◽  
Frederick Kankam ◽  
Edmond Tawiah

Aspergillus flavus is predominant among other fungi species responsible for aflatoxin contamination of crops on the field and in storage. A study was conducted to determine the incidence of Aspergillus flavus in maize seeds from various sources. Sixty (60) maize seed samples were collected from farmers and marketers from different storage structures within Tamale metropolis, Savelugu and Tolon districts,northern region of Ghana. Three different fungi species were isolated from the maize samples. These were Aspergillus flavus, Aspergillus niger and Fusarium spp. Total fungi species differed significantly. Tamale metropolis recorded the least occurrence of fungi species. Aspergillus flavus recorded varied occurrences across all three districts with the highest incidence recorded in Savelugu (42%). Aspergillus flavus was found predominant in maize from the markets than maize from the farmers. The incidences of Aspergillus flavus varied with the method of storage. Storage using cocoa sack recorded the highest incidence (69%), with the lowest (13%) in hanging shed. Aspergillus flavus was however found to be associated with maize from all three districts studied. Proper drying and use of proper storage structures are recommended as a way of combating the high incidences of Aspergillus flavus.


2017 ◽  
Vol 2 (3) ◽  
pp. 129-132
Author(s):  
Aurilio Garcia ◽  
Jobson Bortot ◽  
Marco Antônio Ferraz ◽  
Lívia Mota ◽  
Tassius Borsatto ◽  
...  

Os autores avaliaram o perfil da microbiota conjuntival fúngica de estudantes da área das Ciências da Saúde do Centro Universitário de Volta Redonda – UniFOA. Foram estudados 200 acadêmicos, sendo 100 pertencentes ao ciclo básico, com restrição ao ambiente hospitalar, e 100 do ciclo clínico, com efetivo contato. O material foi coletado do saco conjuntival e semeado em meio de cultura Sabouraud-dextrose-agar, e mantido em temperatura ambiente.As culturas foram positivas em 26 alunos, correspondendo a 13% do total examinado. Foram isolados os seguintes fungos: 7 (26,92%) Candida albicans, 5 (19,23%) Rhodotorula spp., 6 (23,07%) Penicillium spp., 3 (11,53%) Aspergillus niger, 3 (11,53%) Fusarium spp., e 2 (7,69%) Aspergillus fumigatus. Este resultado é compatível com a maioria dos estudos anteriores realizados por outros autores.


2008 ◽  
Vol 1 ◽  
pp. MBI.S943 ◽  
Author(s):  
V.H. Tournas ◽  
E.J. Katsoudas

Various herbal teas including German chamomile, Chrysanthemum Vascuflow herb tea, hop, jasmine and orange flowers, sweet marjoram, spearmint and thyme leaves, and papaya-mint tea as well as coffee substitutes (Bambu instant Swiss, Teeccino chocolate-mint, and Teeccino Mediterranean Espresso) were analyzed for fungal contamination and the presence of aerobic mesophilic bacteria (APC). The results of this investigation showed that fungal counts reached levels as high as 5.8 × 105 colony forming units (cfu) per gram. German chamomile harbored the highest fungal contamination. The most common fungi found in herbal teas were Aspergillus niger, Penicillium spp., Eurotium rubrum, E. chevalieri, A. flavus, Fusarium spp., Alternaria alternata, and yeasts. Among the coffee substitutes, only the chocolate-mint coffee was contaminated with low numbers (<1.0 × 103 cfu g-1) of E. rubrum, Ulocladium spp. and Phoma spp., and with yeasts (< 100–6.8 × 103 cfu g-1). Aerobic mesophilic bacteria were recovered from 100% of the herbal tea, chocolate-mint and Mediterranean Espresso, and from 50% of the Bambu instant Swiss coffee samples. The highest APC counts of 1.2 × 107 cfu g-1 were observed in spearmint leaves.


1970 ◽  
Vol 24 (1) ◽  
pp. 79-80 ◽  
Author(s):  
Irshad Hussain Soomro ◽  
Yasmeen Faiz Kazi ◽  
Miandad Zardari ◽  
Abdul Hussain Shar

One hundred and twenty five soil samples were collected from five areas in the vicinity of Khairpur city, Sindh, Pakistan and screened for the presence of keratinophilic fungi by using hair bait technique. A total 253 isolates belonged to eight genera and eleven species of keratinophilic fungi were recovered. Maximum number (153/253, 60.5%) of keratinophilic fungi was recovered from farm land and poultry soils. Aspergillus niger was most prevalent species and represented 20.2% of the total number isolated. A. flavus and A. fumigatus were almost equal in their prevalence and represented 11.9 and 11.1%, respectively. Botrytis cinaria comes next constituting 9.9%; followed by Mucor spp. (9.1%) and Chochliobolus lunatus (8.3%). Other species isolated less frequently included Chrysosporum asperatum (5.9%), Fusarium spp. (5.1%), Penicillium spp. (3.1%) and A. wentii (2.4%). The distribution pattern of the different keratinophilic fungi and their significance are discussed.Keywords: Keratinophilic fungi, Prevalence, Keratinase, Hair bait techniqueDOI: http://dx.doi.org/10.3329/bjm.v24i1.1246 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 79


2013 ◽  
Vol 13 (2) ◽  
pp. 79-86
Author(s):  
Sadhana Sah ◽  
Ranjita Shrestha ◽  
Sadikchya Koirala ◽  
Keshab Bhattarai

Crude petroleum ether and methanol extracts of different parts of five medicinal plants collected from Terai region, namely, Asparagus racemosus, Catharanthus roseus, Hedychium coronarium, Mimosa pudica and Terminalia chebula were examined for their antimicrobial activity against different pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Klebsella spp., Salmonella paratyphi, Bacillus subtilis, Aspergillus niger, Fusarium spp. and Penicillium spp. From the obtained growth curves of methanol extract of Terminalia chebula and petroleum ether and methanol extracts of Hedychium coronarium, it can be said these extracts have relatively high bacteriostatic property than other plant extracts. Similarly, the zone of inhibitions observed during antifungal assay by methanol extract of Terminalia chebula against Aspergillus niger, Fusarium spp. and Penicillium spp. are comparable to that of Itraconazole (antifungal drug). It shows that Terminalia chebula has fungistatic property. Phytochemical screening revealed the presence of alkaloids, coumarins, flavonoids, terpenoids, steroids and glycosides. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 79-86 DOI: http://dx.doi.org/10.3126/njst.v13i2.7718


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