scholarly journals Distribution of HLA-B Locus Antigens in Patients with Psoriatic Arthritis in Bangladesh

2018 ◽  
Vol 12 (2) ◽  
pp. 14-20
Author(s):  
Devolina Bhowmik ◽  
Md Ruhul Amin Miah ◽  
Shirin Tarafder ◽  
Ahmed Abu Saleh ◽  
Manash Chandra Sarker
Keyword(s):  
Psoriatic Arthritis ◽  
Human Leukocyte ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Specific Primers ◽  
Disease Manifestation ◽  
B Locus ◽  
Leukocyte Antigens ◽  
Healthy Donors ◽  
Polymerase Chain

This study was designed to investigate the distribution of HLA-B locus antigens in patients with psoriatic arthritis (PsA) in Bangladesh and identify HLA markers related to disease manifestation in PsA. HLA-B typing was carried out by polymerase chain reaction (PCR) with sequence specific primers in a group of 50 consecutive PsA patients. The reports of HLA-B locus antigens typing were collected from 50 ages and sex matched unrelated healthy donors as controls. A total of 17 HLA-B locus antigens were determined in both patients and controls. The most common antigen was B*15 (34%) followed by B*07 (26%), B*27 (24%), B*38 (20%). Human leukocyte antigens B*07, B*27 and B*38 alleles were found to be significantly prevalent in PsA patients compared with healthy controls. We found a statistically significant association between spondylitis pattern and the presence of HLA-B*27. PsA in Bangladeshi patients seems to be associated with the presence of B*07, B*27 and B*38 alleles. Bangladesh J Med Microbiol 2018; 12 (2): 14-20

scholarly journals Extração de DNA a partir de sangue humano coagulado para aplicação nas técnicas de genotipagem de antígenos leucocitários humanos e de receptores semelhantes à imunoglobulina

2009 ◽  
Vol 42 (6) ◽  
pp. 651-656 ◽  
Author(s):  
Daniela Maira Cardozo ◽  
Gláucia Andréia Guelsin ◽  
Samaia Laface Clementino ◽  
Fabiano Cavalcante de Melo ◽  
Marco Antônio Braga ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Killer Cell ◽  
Human Leukocyte ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Specific Sequence ◽  
Salting Out ◽  
Leukocyte Antigens ◽  
Polymerase Chain ◽  
Made In

O objetivo deste estudo foi padronizar uma metodologia de extração de DNA de alta qualidade a partir de amostras de sangue coagulado. Quarenta e oito amostras de sangue humano coagulado foram utilizadas para a extração de DNA pelo kit comercial EZ-DNA® (Biological Industries, Beit Haemek, Israel), pelo kit de coluna Neoscience® (One Lambda Inc., San Diego, CA) e pelo método modificado de salting out. Apenas o método de salting out foi capaz de extrair altas concentrações de DNA (média, 180ng/µL), as quais foram medidas pelo detector de fluorescência Qubit® (Invitrogen, USA). Este método permitiu a amplificação dos genes HLA (human leukocyte antigens) pela tecnologia PCR-SSO (polymerase chain reaction - specific sequence of oligonucleotides) Luminex, a qual exige DNA de boa qualidade, e de genes KIR (killer cell immunoglobulin-like receptors) pela técnica made in house PCR-SSP (polymerase chain reaction-sequence specific of primers), a qual demanda uma concentração específica de DNA (10ng/µL). Concluímos que a técnica de salting out modificada foi muito eficiente, simples e rápida para a extração de DNA de amostras de sangue humano coagulado, com o objetivo de realizar a genotipagem de genes HLA e KIR.

Microsporidial infections due toEncephalitozoon intestinalisin non-HIV-infected patients with chronic diarrhoea

2011 ◽  
Vol 140 (10) ◽  
pp. 1773-1779 ◽  
Author(s):  
J. YAKOOB ◽  
Z. ABBAS ◽  
M. ASIM BEG ◽  
W. JAFRI ◽  
S. NAZ ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stool Examination ◽  
Chronic Diarrhoea ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Specific Primers ◽  
Specific Pcr ◽  
Stool Samples ◽  
Polymerase Chain ◽  
Species Specific

SUMMARYWe determined the prevalence of microsporidiaEnterocytozoon(Ent.)bieneusiandEncephalitozoon(E.)intestinalisinfection in patients with chronic diarrhoea and hepatocellular carcinoma (HCC). A total of 330 stool samples were examined from 171 (52%) patients with chronic diarrhoea, 18 (5%) with HCC while 141 (43%) were controls. Stool microscopy, polymerase chain reaction (PCR) with species-specific primers forEnt. bieneusiandE. intestinalisand sequencing were carried out. Microsporidia were found by trichrome staining in 11/330 (3%) andE. intestinalisby PCR in 13/330 (4%) whileEnt. bieneusiwas not detected. PCR forE. intestinaliswas positive in 8/171 (5%) stool samples from patients with chronic diarrhoea, 2/141 (1·4%) samples from healthy controls and in 3/18 (17%) samples from patients with HCC. In the chronic diarrhoea group,E. intestinaliswas positive in 4/171 (2·3%) (P=0·69) stool samples compared to 2/18 (11%) (P=0·06) in the HCC group and 2/141 (1·4%) from healthy controls.E. intestinalisinfection was significantly associated with chronic diarrhoea and HCC in these patients who were negative for HIV. Stool examination with trichrome or species-specific PCR for microsporidia may help establish the cause of chronic diarrhoea.

Human Leukocyte Antigens I and II Haplotypes Associated With Human Papillomavirus 16-Positive Invasive Cervical Cancer in Mexican Women

2009 ◽  
Vol 19 (6) ◽  
pp. 1099-1106 ◽  
Author(s):  
Dulce M. Hernández-Hernández ◽  
Ricardo M. Cerda-Flores ◽  
Teresa Juárez-Cedillo ◽  
Julio Granados-Arriola ◽  
Gilberto Vargas-Alarcón ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Odds Ratio ◽  
Human Leukocyte ◽  
Hla Class I ◽  
Class I ◽  
Human Leukocyte Antigens ◽  
Chain Reaction ◽  
Leukocyte Antigens ◽  
Polymerase Chain

Infection with human papillomavirus (HPV), mainly HPV type 16, is the major etiologic factor associated with cervical cancer (CC), but HPV infection alone is not sufficient for progression of precursor lesions. Host genetic susceptibility may lead to abnormal immune response resulting from virus persistence. Several studies have suggested a possible association with specific human leukocyte antigen (HLA) class I and II alleles and CC, but results are not consistent. The association of genetic HLA class I (A and B) and HLA class II (DR*B1 and DQ*B1) haplotypes with HPV16-positive CC (n = 104) and base population controls (n = 104) was evaluated in this Mexican population study. Sequence-specific primer HLA genes were determined by polymerase chain reaction (PCR)-based methods in peripheral blood cell counts (PCR sequence-specific oligonucleotides). The cervical swabs of 208 women were tested for HPV16 by Hybrid Capture II. Allele and haplotype HLA frequencies, Hardy-Weinberg tests, and a haplotype homogeneity test were estimated using the Arlequin software v. 3.01. Odds ratio (OR) was calculated to compare cases and control women. Consistent associations across other studies in women with CC and infected by HPV16 were observed for HLA-DRB1*15 (OR, 3.9; 95% CI, 1.6-10.2) and the haplotype DRB1*15 DQB1*0602 (OR, 4.1; 95% CI, 1.4-12.7) compared with control women. The HLA-A2-B44-DR4-DQ*0302, HLA-A24-B35-DR16-DQ*0301, and HLA-A2-B40-DR4-DQ*0302 haplotypes showed a positive association with CC (OR, >1), whereas HLA-A2-B39-DR4-DQ*0302, HLA-A24-B35-DR4-DQ*0302, and HLA-A68-B40-DR4-DQ*0302 showed a negative association (OR, <1). These results support the hypothesis that some HLA class I and II haplotypes could be involved with susceptibility for developing CC.Abbreviations:Cervical Cancer-CC, confidence interval-CI, human leukocyte antigens-HLA, human papillomavirus-HPV, odds ratio-OR, polymerase chain reaction-PCR, relative risk-RR, relative light units-RLU, ribonucleic acid-RNA, sequence-sensitive oligonucleotide-SSO

scholarly journals The association of HLA-DQB1*0602 but not HLA-DRB1*15 with obstructive sleep apnea

2017 ◽  
pp. E167-E175
Author(s):  
Suleiman M Momany ◽  
Thamer A Al-Qatarneh ◽  
Yousef S Khader ◽  
Nizar M Abu-Harfeil ◽  
Ammar K Daoud ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Human Leukocyte ◽  
Apnea Hypopnea Index ◽  
Berlin Questionnaire ◽  
Specific Primers ◽  
Leukocyte Antigens ◽  
Obstructive Sleep ◽  
Sleep Breathing Disorder ◽  
Polymerase Chain

Purpose: Obstructive sleep apnea (OSA) is a sleep breathing disorder with unclear multifactorial pathogenesis. This study aimed to investigate the association between OSA and two human leukocyte antigens (HLA) alleles; DQB1*0602 and DRB1*15. Methods: Forty patients with OSA and 40 control subjects were enrolled in the study. OSA diagnosis was made utilizing the Apnea-Hypopnea Index (AHI)≥5 in overnight polysomnography (PSG). AHI was also used to determine OSA severity. Controls were randomly selected from healthy volunteers who had a low risk for OSA, utilizing the Berlin Questionnaire. Polymerase chain reaction (PCR) using Sequence Specific Primers (PCR-SSPs) was used to determine the association between HLA (HLA-DQB1*0602 and HLA-DRB1*15) and OSA, then statistical analyses of the results were performed. Results: HLA-DQB1*0602 allele was found in 85% of all OSA patients and 50% of controls (P< 0.001). In patients with severe OSA, HLA-DQB1*0602 was present in the 92.9% compared with 66.7% in non-severe OSA (P=0.05). HLA-DRB1*15 allele was found in 15% of OSA patients and 20% of controls, with no difference between the two groups (P=0.556). No statistical difference was found in HLA-DRB1*15 between severe and non-severe OSA (P=0.499). After adjusting for gender, HLA-DQB1*0602 allele was associated with increased odds of OSA (OR = 6.17, 95% CI 1.87-20.3, p = 0.003), but HLA-DRB1*15 allele was not associated with OSA (OR 0.45, 95% CI 0.12-1.73, p = 0.242). Conclusions: The presence of HLA-DQB1*0602 allele, but not HLA-DRB1*15 allele, was significantly associated with OSA.

scholarly journals HLA Haplotypes and Genotypes Frequencies in Brazilian Chronic Periodontitis Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Emília Ângela Sippert ◽  
Cléverson de Oliveira e Silva ◽  
Christiane Maria Ayo ◽  
Silvia Barbosa Dutra Marques ◽  
Jeane Eliete Laguila Visentainer ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Human Leukocyte ◽  
Positive Association ◽  
Total Sample ◽  
Periodontal Pathogens ◽  
Chain Reaction ◽  
Predisposing Factor ◽  
Leukocyte Antigens ◽  
Polymerase Chain ◽  
Hla Haplotypes

Human leukocyte antigens (HLA) have a pivotal role in immune response and may be involved in antigen recognition of periodontal pathogens. However, the associations of HLA with chronic periodontitis (CP) have not been previously studied in the Brazilian population. In an attempt to clarify the issue of genetic predisposition to CP, we examined the distribution of HLA alleles, genotypes, and haplotypes in patients from Southern Brazil. One hundred and eight CP patients and 151 healthy and unrelated controls with age-, gender-, and ethnicity-matched were HLA investigated by polymerase chain reaction with sequence specific oligonucleotides. To exclude smoking as a predisposing factor, statistical analyses were performed in the total sample and in nonsmoking individuals. The significant results showed a positive association of the A∗02/HLA-B∗40 haplotype with CP (total samples: 4.2% versus 0%,Pc= 0.03; nonsmokers: 4.3% versus 0%,Pc= 0.23) and a lower frequency of HLA-B∗15/HLA-DRB1∗11 haplotype in CP compared to controls (total samples: 0.0% versus 4.3%,Pc= 0.04; nonsmokers: 0 versus 5.1%,Pc= 1.0). In conclusion, the HLA-A∗02/B∗40 haplotype may contribute to the development of CP, while HLA-B∗15/DRB1∗11 haplotype might indicate resistance to disease among Brazilians.

scholarly journals Γενετικοί πολυμορφισμοί του υποδοχέα της βιταμίνης D και των αντιγόνων HLA σε ελληνικό πληθυσμό. Συσχέτιση με διαταραχές οστικής πυκνότητας

2002 ◽  
Author(s):  
Κωνσταντίνος Δουρούδης
Keyword(s):  
Vitamin D ◽  
Polymerase Chain Reaction ◽  
Human Leukocyte ◽  
Dual Energy ◽  
Human Leukocyte Antigens ◽  
Chain Reaction ◽  
X Ray ◽  
T Score ◽  
Leukocyte Antigens ◽  
Polymerase Chain

Η βιταμίνη D έχοντας ενεργό ρόλο στην ομοιοστασία του ασβεστίου, στην επιμετάλλωση των οστών και στη διαφοροποίηση των οστικών κυττάρων, συμμετέχει στον οστικό μεταβολισμό και ενδέχεται να καθορίζει διαταραχές της οστικής μάζας. Η δράση της βιταμίνης D καθορίζεται μέσω του υποδοχέα της (vitamin D receptor-VDR), το γονίδιο του οποίου εδρεύει στο χρωμόσωμα 12 (12cen-ql2). Παράλληλα, τα Η LA (Human Leukocyte Antigens) αντιγόνα τα γονίδια των οποίων εντοπίζονται στο βραχύ σκέλος του χρωμοσώματος 6 (6ρ21.31), έχουν συσχετισθεί με ποικίλες παθολογικές καταστάσεις ενώ διερευνάται ο ρόλος τους σε νοσήματα του οστικού μεταβολισμού. Οι πολυμορφισμοί στο γονίδιο του υποδοχέα της βιταμίνης D για τις πολυμορφικές θέσεις BsmI, Apal και Taql, και των αντιγόνων Η LA τάξης Ι (-Α, -B, -C) και τάξης II (-DR, -DQ) μελετήθηκαν προκειμένου να εκτιμηθεί ο ρόλος τους στην ελάττωση της οστικής πυκνότητας. Μελετήθηκαν 126 μεταεμμηνοπαυσιακές και 65 προεμμηνοπαυσιακές γυναίκες ελληνικής καταγωγής, οι οποίες, με βάση τα κριτήρια της Παγκόσμιας Οργάνωσης Υγείας και τις τιμές του Τ-score που έφεραν, ύστερα από μέτρηση της οστικής τους πυκνότητας κατατάχθηκαν σε ομάδες φυσιολογικών (ομάδα I, T-score > -1), οστεοπενικών (ομάδα II, -2.5 < T-score < -1) και οστεοπορωτικών (ομάδα III, T-score < -2.5) γυναικών. Η μέτρηση της οστικής πυκνότητας έγινε στο αντιβράχιο με την τεχνική της διπλοενεργειακής απορροφησιομετρίας διπλής δέσμης ακτινών - X, γνωστή ως DEXA (Dual energy X-ray absorptiometry). Δευτεροπαθή αίτια οστεοπενίας -οστεοπόρωσης αποκλείσθηκαν με τον απαραίτητο κλινικό - εργαστηριακό έλεγχο. Η μελέτη των πολυμορφισμών στο γονίδιο του VDR έγινε με τεχνικές μοριακής βιολογίας κάνοντας χρήση της αλυσιδωτής αντίδρασης πολυμεράσης (polymerase chain reaction-PCR) και τη δράση των περιοριστικών ενδονουκλεασών BsmI, Apal και Taql. Για τη μελέτη των ανοσογενετικών δεικτών Η LA χρησιμοποιήθηκε η μικρολεμφοκυτταροτοξική δοκιμασία των δυο σταδίων. Σύμφωνα με την ανάλυση των αποτελεσμάτων στο γονίδιο του VDR, οι γονότυποι ΒΒ και tt και ο απλότυπος BAt χαρακτήριζαν την ομάδα των φυσιολογικών γυναικών, ενώ οι γονότυποι bb, aa και TT και οι απλάτυποι baT και bAT χαρακτήριζαν τις ομάδες των οστεοπενικών - οστεοπορωτικών γυναικών, με διαφορές που ελέγχονται στατιστικά σημαντικές (p<0.05), παρουσιάζοντας σαφή συσχετισμό των VDR αλληλίων με την οστική πυκνότητα. Επίσης, στην ομάδα των μεταεμμηνοπαυσιακών οστεοπορωτικών γυναικών της μελέτης τα αλλήλια ΗLA -Β7, -DQ6(1) και DR15(2) βρέθηκαν με αυξημένη συχνότητα (p<0.05).

scholarly journals AB0691 ANALYSIS OF SARS-COV-2 ANTIBODIES IN NON-COVID-19 PATIENTS: COMPARISON BETWEEN SYSTEMIC SCLEROSIS PATIENTS AND HEALTHY CONTROLS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1379.1-1379
Author(s):  
L. Giardullo ◽  
C. Rotondo ◽  
A. Corrado ◽  
N. Maruotti ◽  
R. Colia ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Systemic Sclerosis ◽  
Autoimmune Disease ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Nuclear Antigen ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Polymerase Chain

Background:Previous study evidenced a cross-reactivity between Sars-Cov-2 antibodies and autoimmune tissue antigen involved in connective tissue diseases, as nuclear antigen (NA), extractable nuclear antigen (ENA), histone and collagen (1). No study has been published about the titer of Sars-Cov-2 antibodies in non-infected patients with autoimmune disease.Objectives:To evaluate the titer of SARS-CoV-2 antibodies in non-COVID-19 patients and compare it between systemic sclerosis (SSc) patients and healthy controls (HC).Methods:A total of 58 patients with SSc (who fulfilled ACR/EULAR 2013 SSc classification criteria) and 18 HC were enrolled. Sera of all participants were collected, and SARS-CoV-2 antibodies (IgG and IgM) were evaluated by means ELISA. In all participants swabs for SARS-CoV-2 by real-time reverse-transcriptase-polymerase-chain-reaction assay were reported negative. Demographic, clinical, and autoimmune serological characteristics of SSc patients were recorded. The normal distribution was assessed using the Shapiro–Wilk’s test. Exclusion criteria was previous or actual Sars-Cov-2 infection. Comparisons between study groups of patients were evaluated by the Student’s t-test or Mann – Whitney U-test as appropriate. The differences between categorial variables were assessed by Pearson chi-square or Fisher’s exact test, as opportune. Statistical significance was set at p ≤ 0.05.Results:We observed significant differences between SSc patients and HC in serum levels of Sars-Cov-2 antibodies (IgG: 1,4±2,1 AU/ml vs 0,36±0,19 AU/ml respectively (p=0,001); and IgM: 2,5±3,1 AU/ml vs 0,8±0,7 AU/ml (p=0,022)). In 5 SSc patients was found titer of Sars-Cov-2 antibodies (IgG) exceeding the cut-off, but the control of swabs for SARS-CoV-2 by real-time reverse-transcriptase-polymerase-chain-reaction assay were negative. No significative differences in Sars-Cov-2 autoantibodies titer were found in subgroup of SSc patients with or without ILD or PAH, limited or diffuse skin subset, and different autoantibodies profile. Furthermore, antibodies titer was not associated with different drugs (steroid, methotrexate, mofetil-mycophenolate and bosentan) in use.Conclusion:A cross mimicking between Sars-Cov-2 antibodies and antinuclear antibodies or anti ENA could be hypothesized. Further studies are necessary to unravel the reliability of Sars-Cov-2 antibodies detection in autoimmune disease.References:[1]Vojdani, A., Vojdani, E., & Kharrazian, D. (2021). Reaction of human monoclonal antibodies to SARS-CoV-2 proteins with tissue antigens: Implications for autoimmune diseases. Frontiers in Immunology, 11, 3679Disclosure of Interests:None declared

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