scholarly journals Antimicrobial sensitivity pattern of Salmonella typhi isolated from blood culture in a referral hospital

2011 ◽  
Vol 5 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Bulbul Hasan ◽  
Sabera Gul Nahar ◽  
Laila Akter ◽  
Ahmed Abu Saleh
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Antimicrobial Susceptibility ◽  
Salmonella Typhi ◽  
Susceptibility Pattern ◽  
Widal Test ◽  
College Hospital ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Sensitivity Pattern

The present study has been carried out in an attempt to evaluate antimicrobial susceptibility patterns with special reference to susceptibility of Salmonella Typhi to ciprofloxacin isolated from blood culture. The study is also designed to find out the MIC of Ciprofloxacin by E- test. Blood samples were taken for culture sensitivity, Widal test and ICT from 100 clinically suspected cases of typhoid fever in 1st week of illness who attended at out patient department of Rajshahi Medical College Hospital (RMCH).The study was done in Microbiology Department of Rajshahi Medical College and Shishu Hospital, Dhaka. Diagnosis of patients was based on history of fever, blood culture, Widal test and ICT. The antimicrobial susceptibility pattern of isolates from blood culture was recorded. Further more, the minimum inhibitory concentration of Ciprofloxacin was determined by E-test for the isolates resistance to Ciprofloxacin. Out of 100 suspected cases of typhoid fever, blood culture positive for S. Typhi were 16 (16%). Antimicrobial susceptibility pattern of 16 isolates of S. Typhi showed that no isolate was resistant to Ceftriaxone and Ceftazidime, only 03(18.75%) were resistant to Ciprofloxacin and Azithromycin whereas 10(62.5%) were MDR showing resistance to Ampicillin, Co-trimoxazole and Chloramphenicol which are first-line antityphoidal drugs. On the other hand, all (100%) the isolates were resistant to Nalidixic acid. The study revealed that Ceftriaxone and Ceftazidime are the most effective drugs in the treatment of typhoid fever. Moreover, E-test has been found to be helpful to determine appropriate therapeutic dose of Ciprofloxacin especially in case of drug resistance and pediatric population.DOI: http://dx.doi.org/10.3329/bjmm.v5i1.15816 Bangladesh J Med Microbiol 2011; 05 (01): 16-20

scholarly journals Pattern of Antimicrobial Susceptibility for Salmonella Typhi in Dhaka Medical College Hospital from 2013-2014

2017 ◽  
Vol 25 (2) ◽  
pp. 115-118
Author(s):  
Sultana Shazeda Nahar ◽  
Aliya Rashid ◽  
Sagana Shahreen Chowdhury ◽  
Ayesha Begum ◽  
Nadira Akter ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Antibiotic Sensitivity ◽  
Salmonella Typhi ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
College Hospital ◽  
Female Ratio ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Sensitivity Pattern

Multidrug resistant Salmonella spp has been emerged as a cause of concern. This study was done to evaluate recent status in antimicrobial susceptibility pattern of Salmonella typhi isolated from blood in Dhaka Medical college Hospital.Methods- The retrospective study was done over a two year period between January 2013 to December 2014 at Microbiology laboratory, Dhaka Medical College. A total of 203 isolates of Salmonella typhi obtained from blood cultures. Both the indoor and outdoor patients were enrolled in this study.Results- During the two year study period, total 203 cases were enrolled. Among them 66% were male with a male to female ratio 2:1. The bulk (55%) cases were in the age group of 15-30 years, 34% cases were in less than 15 years and 10% cases were between the ages of 31-45 years. Regarding antibiotic sensitivity pattern, 90.14% strains were sensitive to amikacin, 90.47% to gentamycin and amoxyclav. 89.16% to doxycycline, 83.75% to cotrimoxazole, 71.42% to azithromycin and 86.2% to ceftazidime. All the Salmonella were sensitive to ceftriaxone, 95.07% were sensitive to ciprofloxacin and 88% were nalidixic acid resistant Salmonella typhi (NARST).Conclusion- Ceftriaxone may be used to treat typhoid fever cases and quinolone has no longer efficacy to treat such cases. Azithromycin may be used as an alternative drug if it is found susceptible in culture and sensitivity testing.J Dhaka Medical College, Vol. 25, No.2, October, 2016, Page 115-118

Antibiotic Sensitivity and Resistance Patterns of Salmonella Typhi in Rajshahi Medical College Hospital

2020 ◽  
Vol 33 (2) ◽  
pp. 10-14
Author(s):  
Md Azizul Haque ◽  
Laila Shamima Sharmin ◽  
KM Faisal Alam ◽  
Md Mohimanul Hoque ◽  
M Morsed Zaman Miah ◽  
...  
Keyword(s):  
Blood Culture ◽  
Enteric Fever ◽  
Antibiotic Sensitivity ◽  
Salmonella Typhi ◽  
Medical College Hospital ◽  
Personal Hygiene ◽  
Care Hospital ◽  
College Hospital ◽  
Medical College ◽  
Sensitivity Pattern

Typhoid and paratyphoid fevers, collectively known as enteric fever, is caused by Salmonella enterica subspecies serovars Typhi and Paratyphi A, B and C. Despite this declining global trend, enteric fever is still considered to be a major public health hazard in Bangladesh and other developing countries due to poor sanitation, inadequate food safety measures and poor personal hygiene. In Bangladesh, the incidence of typhoid fever was reported to be 200 episodes per 100,000 person-years during 2003–2004. Multidrug-resistant (resistance to the first-line antimicrobials ampicillin, cotrimoxazole, and chloramphenicol) strains of S. Typhi and S. Paratyphi are on the rise globally and even cases of extensively drug-resistant (XDR) typhoid cases resistant to chloramphenicol, ampicillin, trimethoprim-sulfamethoxazole, third generation cephalosporins and fluoroquinolones are being reported from many corners of the world. This descriptive, observational study was carried out in Rajshahi Medical College Hospital Hospital, Rajshahi, Bangladesh from July 2017 to June 2019. Antibiotic sensitivity pattern of total 76 cases of enteric fever due to Salmonella Typhi were studied. Blood culture was carried out by BACT ALERT-3D, Automated blood culture analyzer from BioMeriuex SA, France Patented FAN Plus method. Based on the minimum inhibitory concentration (MIC), the organism was categorized as sensitive, intermediate, and resistant against the respective antibiotics as per Clinical and Laboratory Standards Institute (CLSI) criteria. We are reporting antibiotic sensitivity and resistant patterns of S. Typhi documented in Rajshahi Medical College Hospital, a large tertiary care hospital in Northern Bangladesh. TAJ 2020; 33(2): 10-14

scholarly journals Comparative Study of Widal and Typhidot Test in the Diagnosis of Typhoid Fever in Children

2016 ◽  
Vol 36 (2) ◽  
pp. 131-135
Author(s):  
B. Sreenivasa ◽  
G.V. Kumar
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Sensitivity And Specificity ◽  
Age Groups ◽  
Systemic Infection ◽  
Widal Test ◽  
College Hospital ◽  
Medical College ◽  
Life Threatening ◽  
Specific Symptoms

Introduction: Typhoid fever is a life threatening systemic infection that is usually presented with non- specific symptoms and signs. Its diagnosis is usually confirmed by blood culture and Widal test. The delayed results of microbiologic examination and the unreliable results of Widal test makes the search for a rapid and reliable method for diagnosis of typhoid fever. An early and accurate diagnosis is necessary for a prompt and effective treatment. The objectives were to compare the sensitivity and specificity of Widal test and typhidot with the blood culture in the diagnosis of typhoid fever in children.Material and Methods: A total of 200 hundred clinically suspected typhoid fever cases of 2-12 year age groups from both sexes attending Basaveshwara Medical College Hospital Chitradurga were studied. The blood collected aseptically was used for blood culture and clotted blood was used for Widal test and typhidot test.Results: Of 200 blood samples studied, 28 cases were positive by blood culture. Widal test showed sensitivity and specificity of 53% and 83% on blood culture proven cases. Typhidot test showed sensitivity and specificity of 93% and 83%, respectively on blood culture proven cases.Conclusion: Using blood culture as the gold standard, typhidot showed a higher sensitivity compared to Widal test. The specificity of both Widal and typhidot remained the same. Typhidot could be a practical alternative to Widal test in the diagnosis of typhoid fever even in the resource poor laboratories.J Nepal Paediatr Soc 2016;36(2):131-135.

scholarly journals Bacterial Profile of Neonatal Septicemia and Antibiotic Susceptibility Pattern of the Isolates in Tertiary Care Hospital, Dhaka, Bangladesh

2019 ◽  
Vol 43 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Quazi Rakibul Islam ◽  
Mohammod Shahidullah ◽  
Md Zakirul Islam ◽  
Md Al Amin Mridha ◽  
Shahida Akter
Keyword(s):  
Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Neonatal Unit ◽  
Morbidity And Mortality ◽  
Susceptibility Pattern ◽  
Neonatal Septicemia ◽  
Gram Negative ◽  
Antimicrobial Susceptibility Pattern ◽  
Sensitivity Pattern ◽  
Highly Sensitive

Background : Neonatal septicemia (NS) is the most serious problem in special care neonatal unit (SCANU) and neonatal intensive care unit (NICU), resulting in significant morbidity and mortality. We evaluated the causative pathogens and their drug sensitivity pattern in this study, which will certainly help in the choice of specific antibiotic during treatment of septicemic neonates. The objective of this study is to isolate the causative agents of neonatal septicemia and to analyze antimicrobial susceptibility pattern of the isolates. Methodology: This prospective study was carried out from July 2001 to June 2002 in the Neonatal Unit of Bangabandhu Sheikh Mujib Medical University (BSMMU), and Special Care Baby Unit (SCABU) of Bangladesh Institute of Research and Rehabilitation of Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka. Blood samples from 100 clinically suspected NS cases were collected and processed in the microbiology laboratory of BSMMU and BIRDEM. The growths were identified by standard microbiological protocol and their antimicrobial sensitivity pattern was determined. The results were analyzed maintaining standard procedure with SPSS. Results: Of 100 cases, 31 (31%) showed positive blood culture. Gram-negative isolates were 22 (70.97%) and gram-positive 9 (29.03%). Klebsiella pneumoniae was the most common (41.9%), followed by staphylococcus aureus (29%) and E. coli (19.4%) among the isolates. All the three common isolates showed 100% resistance to ampicillin and very poor sensitivity to gentamicin. Gram-negative isolates were highly sensitive to amikacin and imipenem, where as gram-positive isolates were highly sensitive to amikacin and vancomycin. Conclusion: Neonatal septicemia was found to be 31% in this study, based on blood culture, as the gold standard investigation for diagnosis. A change in microbial spectrum and change in their antimicrobial susceptibility pattern as noticed in this study will certainly help in treating such cases with appropriate antibiotic and thereby help to decrease neonatal morbidity and mortality. Bangladesh J Child Health 2019; VOL 43 (1) :35-40

scholarly journals Study of Clinical Profile and Antibiotic Response in Typhoid Fever at Faridpur Medical College Hospital

2017 ◽  
Vol 12 (1) ◽  
pp. 2-4
Author(s):  
Aloke Kumar Saha ◽  
Md Kamrul Hassan ◽  
Lakshman Chandra Kundu ◽  
Shyamal Kumar Saha ◽  
Poly Begum
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Generation Cephalosporin ◽  
Response To Therapy ◽  
Medical College Hospital ◽  
Clinical Profile ◽  
College Hospital ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Antibiotic Response

The objectives of this present study is to evaluate the clinical profile and pattern of various drugs used in the treatment of typhoid fever. A retrospective analysis of Paediatric patients suffering from typhoid fever was done at Department of Paediatrics, Faridpur Medical College Hospital, Faridpur during the year 2015-2016. Diagnosis of patients was based on clinical features, Widal test and blood culture. The sensitivity pattern of isolates from blood culture was recorded. The mode of presentation, clinical course, treatment history, laboratory investigations reports, antibiotic administered, response to therapy and the complications were recorded. Total number of 50 cases of typhoid fever were studied. Out of these 23 (46%) were males and 27 (54%) were females. Average age of presentation was 8.2 years. Average duration of hospital stay was 10.8 days. Fever was present in all patients. Resistance of S. typhi to amoxicillin, chloramphenicol, ampicillin and co-trimoxazole were significantly high. Ciprofloxacin also showed resistance in 21.4% of cases. Sensitivity to cephalosporin was 100% in our study. Ciprofloxacin was the most commonly used antibiotic in our study (26 patients). Chloramphenicol alone was used in 2 patients and in 3 patients it was given after 6 days of ciprofloxacin treatment. Third generation cephalosporin (ceftriaxone) alone were used in 10 patients. Indiscriminate use of drugs in typhoid fever should be discouraged. Appropriate antibiotic as indicated by sensitivity tests should be employed to prevent the development of resistant strains of S. typhi.Faridpur Med. Coll. J. Jan 2017;12(1): 2-4

scholarly journals Isolation and Antimicrobial Susceptibility Pattern of Urinary Escherichia Coli in Dhaka Medical College Hospital, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 40-44
Author(s):  
Tashmin Afroz Binte Islam ◽  
SM Shamsuzzaman ◽  
Irin Rahman ◽  
Faria Ferdousi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Test Reaction ◽  
College Hospital ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

Background: Escherichia coli is the major bacterial pathogen being isolated and reported from urine, globally. For these uropathogens the therapeutic management becomes limited, so knowledge about the antimicrobial resistance pattern of these agents at a specific area may help the doctors to choose correct treatment regimen. The aim of the current study was to detect the common uropathogens from urine and the antimicrobial susceptibility pattern of Escherichia coli in Dhaka Medical College Hospital (DMCH).Methods: Samples were collected over a period of 12 months from July 2011 to June 2012 from the patients of DMCH irrespective of age and sex. Samples were cultured in blood agar and MacConkey agar media and organisms were identified by different biochemical tests such as oxidase test, reaction in MIU and simmon's citrate media and different sugar fermentation tests. Data were analyzed using Microsoft Excel (2007).Results: From total 300 urine samples, 177 (59%) Gram-positive and Gram-negative bacteria were isolated. Among them, Esch. coli was the most predominant 112 (63.28%) organism followed by Enterobacter spp. (11.87%). The prevalence of Esch. coli was significantly higher in females (63.39%) of younger age group than in males (36.61%). Esch. coli was highly sensitive to imipenem (100%) followed by ceftriaxone (62.50%), ceftazidime (55.36%) and azitromycin (35.72%) respectively. Low sensitivity patterns were found against doxycycline (13.39%), co-trimoxazole (16.07%), amoxiclav (21.43%) and ciprofloxacin (23.22%) respectively.Conclusion: The result of this study provides insight into the high proportion of multidrug resistant Escherichia coli and creates a need to report them routinely in laboratories.Anwer Khan Modern Medical College Journal Vol. 7, No. 1: Jan 2016, P 40-44

scholarly journals Antimicrobial susceptibility pattern of pathogenic bacteria of Chronic Suppurative Otitis Media (CSOM) patients through culture and sensitivity in a tertiary level hospital in Khulna

Mediscope ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 1-6
Author(s):  
Mitun Kumar Paul ◽  
Baishakhi Islam ◽  
KM Reza Ul Haq
Keyword(s):  
Otitis Media ◽  
Antimicrobial Susceptibility ◽  
Pathogenic Bacteria ◽  
Chronic Suppurative Otitis Media ◽  
Susceptibility Pattern ◽  
College Hospital ◽  
Suppurative Otitis Media ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Culture And Sensitivity

Background: Chronic Suppurative Otitis Media (CSOM) is a chronic disease associated with irreversible consequences and serious intracranial and extracranial complications. Thereby early & effective treatment must be needed to avoid such complications. Objectives: This study was carried out to know antimicrobial susceptibility pattern of pathogenic bacteria through culture and sensitivity for better management and to reduce resistance & morbidity due to CSOM. Methods: After taking proper approval from hospital administration, this study was conducted on 82 patients of clinically diagnosed cases of both Tubo-tympanic & Attico-antral variety of CSOM attending ENT OPD of Gazi Medical College Hospital, Khulna from January 2018 to June 2018. After proper sample collection by sterile aural swabs, they were immediately sent to the microbiology laboratory of Gazi Medical College Hospital, Khulna for bacterial culture, isolation and identification. Routine antibacterial susceptibility was done as per CLSI guidelines. SPSS 18.0 was used for statistical analysis. Results: The commonest pathogens isolated were Staphylococci, Coagulase Negative Staphylococci (CONS), Pseudomonas aeruginosa, Klebsiella spp. & others; mostly showing susceptibility to high end antibiotics like Ceftriaxone and Amoxiclav for staphylococcal infection & piperacillin-tazobactum for Pseudomonal infection. Conclusion: Antibiotic sensitivity pattern determines the prevalent bacterial organism causing CSOM to start empirical treatment for a successful outcome, and thus to prevent the emergence of resistant strains. Mediscope Vol. 7, No. 1: Jan 2020, Page 1-6

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