scholarly journals Clinico-Histopathological histopathological correlation and detection of anti PGL-1 antibodies in leprosy patients

2014 ◽  
Vol 13 (3) ◽  
pp. 316-322
Author(s):  
Soma Datta ◽  
SP Pandya ◽  
YR Marfatia ◽  
Arindam Rakshit
Keyword(s):  
Blood Donors ◽  
Medical Science ◽  
Pathological Examination ◽  
Adequate Treatment ◽  
Smear Examination ◽  
Histopathological Correlation ◽  
Histopathological Evaluation ◽  
Leprosy Patients ◽  
Preclinical Diagnosis ◽  
Monitoring Treatment

Background: Leprosy (Hansen’s disease) is one of the major health problems of the world especially in developing countries. In India, it was first described in “Sushruta Samhita” & treated by Chaulmoogra oil and caused by Mycobacterium Leprae. Early diagnosis of leprosy, an absolute necessity for control as well as effective therapy. For this, clinical diagnosis, skin smear examination is adequate coupled with histo-pathological examination of skin and nerve lesions with modified Fite Faraco stain for demonstration of acid-fast bacilli. Moreover, bacillary index is required for adequate combined chemotherapy regimen. Detection of anti PGL-1 antibodies in serum gives an added advantage for detection and monitoring treatment. Materials & Methods : A total of 85 cases of leprosy who attended outpatient department of Skin & VD, Shri Sayaji General Hospital Baroda chosen for study during ‘07-08’ period with 75 cases from leprosy hospital, Baroda which included 50 detected patients and 25 child contacts with 25 healthy voluntary blood donors from blood bank, SSGH selected. Clinical, past and family history taken with slit skin smears stained with Z-N stain, graded and histopathological evaluation done. Serological study done from serum of leprosy patients and healthy blood donors; tested by Serodia Kitsand interpretation made. Results: Most cases were in 2nd to 4th decade and males dominated. Clinically most cases were of indeterminate and tuberculoid type and histologically indeterminate and border-line tuberculoid. Clinico-histopathological correlation was found most in indeterminate followed by histoid type. Voluntary blood donors were seronegative. 21 out of 48 multibacillary cases and 6 out of 28 pauci-bacillary showed seropositivity for anti PGL-1 anti-bodies (p<0.001) Conclusion: All suspected leprosy cases clinically should be subjected to slit skin smear examination with histopathological evaluation; bacillary study which helps in diagnosis and adequate treatment of patients. Detection of antibodies to PGL-1 in patients indicate presence of leprosy bacilli and useful in preclinical diagnosis and determining progress of therapy. DOI: http://dx.doi.org/10.3329/bjms.v13i3.9153 Bangladesh Journal of Medical Science Vol.13(3) 2014 p.316-322

scholarly journals Spectrum of Leprosy Patients with Clinico-Histopathological Correlation: A Hospital Based Study

2013 ◽  
Vol 4 (4) ◽  
pp. 11-16
Author(s):  
Arjun Singh ◽  
R Gaur ◽  
R Ambey
Keyword(s):  
Histopathological Examination ◽  
Medical Science ◽  
Polar Group ◽  
Female Ratio ◽  
Histopathological Correlation ◽  
Leprosy Patients ◽  
Diagnosis And Classification ◽  
Skin Biopsies ◽  
Hematoxylin And Eosin ◽  
Minor Disagreement

Introduction: Leprosy is a chronic granulomatous, infectious disease involving skin and peripheral nerves. It is present in various clinicopathological forms depending upon immune status of the patients. Histopathological examination of skin provides confirmatory diagnosis in suspected cases and gives indication of progression and regression of disease under treatment. Ridley and Jopling classification is used to classify leprosy. The objective of study was to identify the clinical pattern of leprosy and performed detail clinico- histopathological correlation in our institute. Method: The study was carried out on the skin biopsies received in between 2007-2010. Biopsies were fixed in 10% formalin, processed and stained with Hematoxylin and Eosin, modified Fite Ferraco and Ziehl-Neelsen stains. The predesigned Performa was used to record observation. The clinical diagnosis were correlated with histopathology in all 120 cases. Result: The age of the patients was ranged from 8 to 79 years with mean age of 36.38 years. The male to female ratio of patients was 1.5 to 1. The majority of cases 79 (65.8%) were in the age group of 21-50 years. Highest parity was observed in stable polar group TT 100%. Clinco-histopathological agreement was seen in 98 (81.67%) cases, 14 (11.67%) cases shows minor disagreement and 8 (6%) cases major disagreement. Conclusion: The clinical and histopathological features along with bacteriological index are useful than any single parameter in arriving definitive diagnosis and classification of the leprosy. Asian Journal of Medical Science, Volume-4 (2013), Pages 11-16 DOI: http://dx.doi.org/10.3126/ajms.v4i4.7997 

TO STUDY THE CYTOMORPHOLOGICAL PATTERNS OF TUBERCULAR LYMPHADENOPATHY AND ITS HISTOPATHOLOGICAL CORRELATION

2021 ◽  
pp. 26-27
Author(s):  
Rashmi Sharma ◽  
Poonum Gogania ◽  
Geeta Pachori ◽  
Akhilesh Bharadwaj
Keyword(s):  
Epithelioid Cell ◽  
Cervical Region ◽  
Tuberculous Lymphadenitis ◽  
Female Ratio ◽  
Caseous Necrosis ◽  
Histopathological Correlation ◽  
Histopathological Evaluation ◽  
Special Stain ◽  
Hematoxylin And Eosin ◽  
Tubercular Lymphadenitis

INTRODUCTION: Tubercular lymphadenopathy is one of the common clinical problems. Practically diagnosis of tuberculosis sometimes creates a challenge to the pathologist and clinician. A combined approach of cytology with aid of special stain and histopathological evaluation helps to conrm the diagnosis. AIM -The present study was carried out to evaluate the different cytological patterns of tuberculous lymphadenitis along with utility of special stain like Acid fast stain and further histopathological evaluation. MATERIAL AND METHOD: Smears from 833 cytologically diagnosed cases of tubercular lymphadenitis were prepared and stained with Hematoxylin and Eosin (H&E), Giemsa and Acid-fast stain. All the smears were categorized into four cytomorphological patterns and correlated with 250 histopathologically available cases. RESULT: Tuberculosis is the major cause of lymphadenopathy. Majority of cases (63 %) were in their second to third decades of life, with male to female ratio of 1:1.4. Cervical region was the most common site of involvement (83.5 %). Smear revealed epithelioid granulomas with caseous necrosis in maximum cases (46.9 %). AFB positivity was seen highest in smear revealing necrosis only with or without epithelioid cell (93,8 %). histopathological correlation was seen in 248 cases out of 250 available cases. CONCLUSION: FNAC has been proved very safe, highly sensitive, and rst line investigation in diagnosing tubercular lymphadenitis. The approach to tubercular lymphadenitis attains completeness with cytopathological, Acid Fast stain and histopathological evaluation.

scholarly journals Automated Low Cost Method of Quantification of Hepatitis C Virus

2015 ◽  
Vol 14 (3) ◽  
pp. 247-253
Author(s):  
Faiz MMT Marikar ◽  
Dammika Senevirathna ◽  
Neil Fernandopulle
Keyword(s):  
Viral Load ◽  
Hepatitis C ◽  
Blood Donors ◽  
Low Cost ◽  
Medical Science ◽  
Simultaneous Detection ◽  
Plasma Samples ◽  
Rt Pcr ◽  
Viral Genomes ◽  
Post Therapy

This paper describes the development of a Dig-dUTP based multiplex real time RT-PCR for the simultaneous detection of HCV viral amount in plasma samples. Viral genomes were identified in the same sample by Dig-dUTP PCR 216 bp region. Analysis of known scalar concentrations of reference plasma indicated that the multiplex procedure detects at least 500 copies/ml of HCV. In addition, we also assayed HCV viral load in eighty co-infected patients and in fifteen blood donors, confirming the sensitivity and specificity of the assay. This method may represent a useful alternative method for the detection of HCV co-infection, reliable for a rapid and relatively inexpensive screening of blood donors. The assay may be used to determine post-therapy viral clearance.Bangladesh Journal of Medical Science Vol.14(3) 2015 p.247-253

scholarly journals Significance of Histopathology in Leprosy Patients with 1–5 Skin Lesions with Relevance to Therapy

2011 ◽  
Vol 3 (01) ◽  
pp. 021-024 ◽  
Author(s):  
S Veena ◽  
Prakash Kumar ◽  
Shashikala P. ◽  
Gurubasavaraj H. ◽  
H R Chandrasekhar ◽  
...  
Keyword(s):  
Drug Therapy ◽  
Skin Lesion ◽  
Skin Lesions ◽  
Clinicopathological Correlation ◽  
Adequate Treatment ◽  
Duration Of Therapy ◽  
Tuberculoid Leprosy ◽  
Leprosy Patients ◽  
Skin Biopsies ◽  
Severity Of The Disease

ABSTRACT Background: Patients with 1-5 skin lesions are clinically categorized as paucibacillary for treatment purposes. For betterment and adequate treatment of patients, this grouping needs further study. Aim: To study a group of leprosy patients with 1-5 skin lesions, compare clinical details with histopathological findings and bacteriological status of the skin to evaluate the relevance of this grouping. Materials and Methods: Two-year study involving 31 patients of leprosy with 1-5 skin lesions was included in this study. A number of skin lesions were recorded. Skin biopsies were taken in all patients. The biopsies were evaluated for the type of pathology and acid fast bacilli (AFB) status. Results: Of 31 patients, 19 (61.2%) had single skin lesion, 7 (22.5%) had two lesions, 4 (12.9%) had three lesions, and only one (3.22%) had four lesions, there were no patients with five lesions. Of the 31 patients, 30 (96.7%) were clinically diagnosed as borderline tuberculoid and one patient (3.22%) has tuberculoid leprosy. Skin smears were negative for AFB in all patients. The histological diagnoses were: TT 1 (3.22%), BT 24 (77.41%), and IL 6 (19.2%). AFB were found in 2 (6.45%) out of 31 skin biopsies. Clinicopathological correlation was 76.6% in the BT group. Conclusion: Tissue biopsy findings in 1-5 skin lesions which were not considered relevant for treatment purposes until now should be given a status in the categorization and assessment of severity of the disease. The significance of finding of AFB and histopathology of multibacillary (MB) type of leprosy in tissue biopsies, in patients grouped as PB should be resolved so that patients could be given the drug therapy and duration of therapy they warrant.

scholarly journals Pathological Examination of the Lung in Leprosy Patients.

1997 ◽  
Vol 66 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Katsuya Chinen ◽  
Iwao Nakazato ◽  
Atsushi Hosokawa ◽  
Satoshi Sawada
Keyword(s):  
Pathological Examination ◽  
Leprosy Patients

scholarly journals Frequency of Hepatitis B Virus carrier by Detecting AntiHBc Antibody among HBsAg Negative Blood Donors

2016 ◽  
Vol 15 (1) ◽  
pp. 66-69
Author(s):  
Kashfia Islam ◽  
Mazharul Hoque ◽  
Samsun Nahar ◽  
Syed Dawood Md. Taimur ◽  
Zeenat Farzana Rahman ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Blood Donation ◽  
Hepatitis B Surface Antigen ◽  
Medical Science ◽  
Chromatography Method ◽  
Cross Sectional ◽  
B Virus ◽  
History Of

Background & Objective: Transfusion associated hepatitis B virus (TAHBV) infection continues to be a major problem despite mandatory screening for hepatitis B surface antigen (HBsAg). This is because HBsAg is not detected during the window period of the infection. This study was designed to assess the frequency of anti HBc antibody among HBsAg negative donor and also to determine the demographic profile of healthy blood donors.Material & Method: This cross sectional observational study was carried out in the Department of Transfusion Medicine, BSMMU, Dhaka during the period of May 2014 to April 2015. A total number of 100 consecutive healthy blood donors who were clinically and physically healthy and serologically found to be free from HBsAg, HCV and HIV by rapid chromographic test were included in this study.Results: Within the 100 patients among them history of jaundice was found in 17(17.0%). There all (100.0%) subjects had negative HBsAg and 14(14.0%) subjects had positive anti HBc-Ab. Anti HBc-Ab was statistically significant (p<0.001) in Z-test.Conclusion: This study was undertaken to assess the prevalence of anti HBc antibody among healthy blood donors with HBsAg negative in rapid chromatography method. The prevalence of anti HBc antibody was about 14 (14.0%) among 100 HBsAg negative blood donors. History of blood donation was found in almost two third 65 (65.0%). So, anti HBc antibody screening is an useful tool for estimating the risk of transfusion transmitted HBV infection.Bangladesh Journal of Medical Science Vol.15(1) 2016 p.66-69

scholarly journals Child with Mediastinal cavernous lymphangioma

2012 ◽  
Vol 11 (4) ◽  
pp. 256-358
Author(s):  
Naffisa Adedin ◽  
Abdullah Shahriar ◽  
Jafreen Sultana ◽  
Nayeema Rahman ◽  
Nusrat Ghafoor
Keyword(s):  
Mediastinal Mass ◽  
Lymphatic System ◽  
Medical Science ◽  
Male Child ◽  
Pathological Examination ◽  
Low Density ◽  
X Ray ◽  
Chest X Ray ◽  
Rare Lesion ◽  
Surgical Sample

Mediastinal cavernous lymphangioma is a benign rare lesion originating from lymphatic system. It is usually asymptomatic. We have presented a 2 year old male child with fever for seven days. Opacity was found in chest X-ray in the upper part of right hemithorax, merged with the mediastinum.CT scan of chest was performed, which revealed a large, lobulated, smoothly marginated non-enhancing, low density, mediastinal mass, involving right half, extending from root of neck. Finally, pathological examination of the surgical sample indicated ?Cavernous lymphangioma’. DOI: http://dx.doi.org/10.3329/bjms.v11i4.12612 Bangladesh Journal of Medical Science Vol. 11 No. 04 Oct’12  

scholarly journals Postoperative complicated appendectomy revealing Crohn’s disease in a pediatric patient

2021 ◽  
Vol 8 (1) ◽  
pp. 154-160
Author(s):  
Augustina Enculescu ◽  
Mircea Lupusoru ◽  
Catalin Cirstoveanu ◽  
Andra-Iulia Suceveanu ◽  
Liliana Andronache ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Acute Appendicitis ◽  
Histopathological Examination ◽  
Pediatric Population ◽  
Pathological Examination ◽  
Ileocecal Resection ◽  
Initial Manifestation ◽  
Adequate Treatment ◽  
Granulomatous Appendicitis

Granulomatous appendicitis is a very rare entity in children. It is related to Crohn’s disease in only 5-10% of the cases. The diagnosis of Crohn’s disease is a real challenge in the pediatric population, especially when its initial manifestation is acute appendicitis. Herein, we present a 13-year-old boy admitted for acute appendicitis. The histopathological examination was conclusive for acute granulomatous appendicitis. The development of an entero-cutaneous fistula has complicated the postoperative evolution. The failure of the conservative management required ileocecal resection. The pathological examination revealed Crohn’s disease. The one-year follow-up on the patient din not reveal any complications. We strongly support the importance of the histopathological examination of the entirely removed appendix. Careful assessment of granulomatous appendicitis is needed in order to establish its possible etiology and to initiate the adequate treatment.

Was it thickened Dua’s layer? Clinical, tomographical, and histopathological correlation: A case report

2020 ◽  
pp. 112067212097427
Author(s):  
Vijay K Sharma ◽  
Rajesh Sinha ◽  
Alok Sati ◽  
Manisha Agarwal
Keyword(s):  
Case Report ◽  
Cataract Surgery ◽  
Trypan Blue ◽  
Internal Limiting Membrane ◽  
Bullous Keratopathy ◽  
Endothelial Keratoplasty ◽  
Internal Limiting Membrane Peeling ◽  
Histopathological Correlation ◽  
Pseudophakic Bullous Keratopathy ◽  
Histopathological Evaluation

A 62-year-old female developed pseudophakic bullous keratopathy after cataract surgery and underwent Descemet stripping automated endothelial keratoplasty (DSAEK). Intraoperatively, a white opacified pre-Descemet’s layer was noticed after scoring and removal of trypan blue stained Descemet’s membrane (DM), and endothelium complex. It was removed using internal limiting membrane peeling forceps and sent for histopathological evaluation along with DM-endothelium complex and epithelium. Preoperative, intraoperative, and postoperative clinical, tomographical, and histopathological data confirmed thickened PDL or Dua’s layer. This is a rare encounter with scarred PDL or Dua’s layer during DSAEK surgery.

Factors Associated With Single and Recurrent Bacteremia in Childhood Brucellosis

2019 ◽  
Author(s):  
Shalom Ben-Shimol ◽  
Salar Farahvar ◽  
Yariv Fruchtman ◽  
Naphtali Justman
Keyword(s):  
Multivariate Analyses ◽  
Univariate Analysis ◽  
Immunoglobulin M ◽  
Prolonged Treatment ◽  
High Grade Fever ◽  
Adequate Treatment ◽  
Elevated Liver Enzymes ◽  
Poor Treatment ◽  
Monitoring Treatment ◽  
Culture Negative

Abstract Background Data regarding risk factors of bacteremic brucellosis, and specifically recurrent bacteremia, are scarce. We assessed patients with childhood brucellosis, differentiating between those having culture-negative, single-bacteremic, and recurrent-bacteremic episodes. Methods The medical files of pediatric brucellosis patients, from 2005 through 2014, were reviewed retrospectively. Univariate and multivariate analyses were performed to compare demographic and clinical characteristics of culture-negative, single-bacteremic, and recurrent-bacteremic (≥30 days between positive cultures) episodes. Results Of all 436 brucellosis cases, 22% were culture-negative, 72% were single-bacteremic, and 6% were recurrent-bacteremic. In a univariate analysis, single-bacteremic episodes were associated with fever (90% vs 65% and 40% in culture-negative and recurrent bacteremia, respectively) and elevated glutamic oxaloacetic transaminase (GOT) levels. Recurrent-bacteremic episodes were associated with anemia (26% vs 14% and 9% in single bacteremia and culture negative, respectively), elevated GOT, low immunoglobulin M (IgM) titers (56% vs 89% and 99%, respectively), and lower levels of adequate treatment (74% vs 94% and 86%, respectively). In multivariate analyses, single bacteremia was associated with fever (odds ratio [OR], 3.595, compared with culture negative), while recurrent bacteremia was inversely associated with IgM titers ≥1:160 (OR, 0.022 and 0.226 compared with culture negative and single bacteremia, respectively) and fever (OR, 0.108 compared with single bacteremia). Conclusions Brucellosis episodes are commonly complicated with bacteremia. Single-bacteremic episodes were associated with high-grade fever and elevated liver enzymes, possibly indicating high bacterial virulence. Recurrent-bacteremic episodes were associated with poor treatment at initial diagnosis, along with low rates of fever, low IgM titers, and high anemia rates, possibly indicating impaired host response. Physicians should consider treatment modifications for suspected recurrent-bacteremic brucellosis, including monitoring treatment adherence, and possibly administering prolonged treatment.

Sign in / Sign up

Export Citation Format

Share Document