scholarly journals In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi Linn.

1970 ◽  
Vol 6 (2) ◽  
pp. 197-202 ◽  
Author(s):  
MR Saha ◽  
SMR Hasan ◽  
R Akter ◽  
MM Hossain ◽  
MS Alam ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Reducing Power ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Mimusops Elengi ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay ◽  
Dose Dependent

In the present study, antioxidant potential of the methanol extract of the leaves of Mimusops elengi Linn. was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activities in all antioxidant assays compared to the reference antioxidant ascorbic acid in a dose dependent manner. In DPPH scavenging assay the IC50 value of the extract was found to be 43.26μg/ml while the IC50 value of the reference standard ascorbic acid was 58.92 μg/ml. Total antioxidant activity was also found to increase in a dose dependent manner. Moreover, M. elengi extract showed strong reducing power. These results suggest that Mimusops elengi may act as a chemopreventative agent, providing antioxidant properties and offering effective protection from free radicals. Key words: Mimusops elengi, antioxidant, reducing power, total antioxidant capacity, reactive oxygen species   doi: 10.3329/bjvm.v6i2.2336 Bangl. J. Vet. Med. (2008). 6 (2): 197-202

scholarly journals Antioxidant, cytotoxic and analgesic activities of the methanolic fruit extract of Terminalia chebula Retz.

2013 ◽  
Vol 3 (1) ◽  
pp. 219-222
Author(s):  
Shammy Sarwar ◽  
Asif Hasan Malik ◽  
Muhammad Ashikur Rahman ◽  
Md. Zakiur Rahman ◽  
Md. Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Ascorbic Acid ◽  
Cytotoxic Activity ◽  
Reducing Power ◽  
Dependent Manner ◽  
Terminalia Chebula ◽  
Reference Drug ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Dose Dependent

The present study was aimed to investigate antioxidant, analgesic and cytotoxic activity of the methanolic extract of Terminalia chebula Retz. fruits. Antioxidant potential of the extract was evaluated by using nitric oxide scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activities in all antioxidant assays compared to ascorbic acid in a dose dependent mode. In nitric oxide scavenging assay, the IC50 value of the extract was found to be 51.3 µg/mL while the IC50 value of ascorbic acid was 77.4 µg/mL. In addition to strong reducing power, total antioxidant activity of the extract was also found to increase in a dose dependent manner. The analgesic activity was evaluated using acetic acid-induced writhing test in mice. The extract, at a dose of 500 mg/kg, showed a maximum of 44.17 % inhibition (p < 0.05) of writhing reaction compared to the reference drug diclofenac-sodium (66.96 %). The extract also showed moderate cytotoxic activity in brine shrimp lethality bioassay and the LC50 value was found to be 97.36 µg/mL.DOI: http://dx.doi.org/10.3329/icpj.v3i1.17296 International Current Pharmaceutical Journal, December 2013, 3(1): 219-222

scholarly journals Evaluation of Analgesic and Antioxidant Potential of the Leaves of Curcuma alismatifolia Gagnep.

1970 ◽  
Vol 1 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Raushanara Akter ◽  
SM Raquibul Hasan ◽  
Samira Ayesha Siddiqua ◽  
Muntasir Mamun Majumder ◽  
Md Mokarram Hossain ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Dependent Manner ◽  
Writhing Test ◽  
Ic50 Value ◽  
Tail Immersion ◽  
Total Antioxidant ◽  
Dose Dependent

In the present study, the antioxidant and analgesic potential of the 80% methanol extract of the leaves of Curcuma alismatifolia Gangnep was evaluated. The extract was investigated for its antioxidant activity using lipid peroxidation, total antioxidant capacity and reducing power assays. The extract showed significant antioxidant activities in lipid peroxidation assay compared to the standard antioxidant in a dose dependent manner. In lipid peroxidation assay, the IC50 value was found to be 122.43μg/mL while the IC50 value for the reference ascorbic acid was 147.87μg/ml. Moreover, Curcuma alismatifolia extract showed strong total antioxidant capacity and good reducing power. The analgesic activity was evaluated for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test in mice respectively. The extract, at the dose of 250 and 500 mg/kg, produced a significant (p < 0.05-0.001) increase in pain threshold in tail immersion methods in a dose dependent manner. In acetic acid-induced writhing test the extract, at a dose of 500 mg/kg, showed a maximum of 60.5% inhibition (p < 0.001) of writhing reaction compared to the reference drug diclofenac-sodium (75.0%). All experimental results suggest the use of this plant for the treatment of pain and inflammatory disorder. Key Words: Curcuma alismatifolia, Antioxidant, Lipid peroxidation, Total antioxidant capacity, Reducing power, Analgesic, Tail immersion. doi:10.3329/sjps.v1i1.1779 S. J. Pharm. Sci. 1(1&2): 3-9

scholarly journals Antioxidant and cytotoxic activities of Ageratum conyzoides stems

2013 ◽  
Vol 2 (2) ◽  
pp. 33-37 ◽  
Author(s):  
Fatema Nasrin
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ageratum Conyzoides ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Total Antioxidant

Modern civilization is facing more than hundreds of disorders associated with free radicals and natural antioxidants from non-edible plants are gaining importance to fight these disorders. The intention of this report is to evaluate a well known medicinal weed Ageratum conyzoides stems for its antioxidant and cytotoxic Effects. Antioxidant potentiality of the crude methanolic extract of the Ageratum conyzoides (AC) stems was investigated on DPPH scavenging activity, reducing ability, total antioxidant capacity as well as total phenolic contents. Cytotoxic study was done by brine shrimp lethality bioassay and vincristin sulphate was used as standard. The total phenols and total antioxidant capacity of AC was found to be 38.125 ± 2.01mg/g equivalent of gallic acid and 333.37 ± 4.22mg/gm equivalent of ascorbic acid, respectively. The percentage (%) scavenging of DPPH free radical of the extract was found to be concentration dependent with IC50 value 46.01 ± 2.23µg/ml while IC50 value of standard ascorbic acid was found to be 29.56 ± 0.11?g/ml. The reducing power of AC was found to be concentration dependent. The cytotoxicity exhibited by AC was found promising with LC50 value 1.32?g/ml, comparing with the LC50 (0.689?g/ml) values of vincristin sulphate. The present investigation suggests that Ageratum conyzoides possesses remarkable antioxidant and cytotoxic property.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13195 International Current Pharmaceutical Journal 2013, 2(2): 33-37

scholarly journals Biological Activities of Bellis perennis Volatiles and Extracts

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Neslihan Kavalcioğrlu ◽  
Leyla Açık ◽  
Fatih Demirci ◽  
Betül Demirci ◽  
Hülya Demir ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Plant Materials ◽  
Total Antioxidant ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging

Different parts of Bellis perennis were subjected to hydrodistillation and the products were subsequently analyzed by GC and GC/MS. Aqueous and methanol extracts were prepared from the aerial parts. Antioxidant [1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, reducing activity, total antioxidant] and antimicrobial activity of the plant materials were studied. The aqueous extracts showed higher DPPH scavenging activity (85.8% at 102.5 μg/mL) than the methanol extract. Reducing power was also observed for both tested extracts, where the formation of linoleic acid peroxides was more for the aqueous extract than the methanol extract. Antimicrobial activities of all plant materials were evaluated at varying concentrations against a panel of pathogens, but without pronounced inhibitory effect.

scholarly journals Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

1970 ◽  
Vol 3 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Fatema Tuz Zohera ◽  
Md Razibul Habib ◽  
Mohammad Zafar Imam ◽  
Md Ehsanul Hoque Mazumder ◽  
Md Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

scholarly journals Canthium parviflorum Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vanitha Reddy Palvai ◽  
Sowmya Mahalingu ◽  
Asna Urooj
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Temperature Stability ◽  
Dependent Manner ◽  
Oil Emulsion ◽  
Dose Dependent

Canthium parviflorum leaves were analyzed for their proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent induced oxidation in oil emulsion and microsomes. In addition, the effect of high temperature (100°C, 15 and 30 min) and pH (4.5, 7, and 9) on the antioxidant activity of ME was investigated. The leaves were rich in polyphenols, flavonoids β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose dependent manner. The RSA was 68%–500 μg. Reducing potency was 0.34 and FRAP was 1.377. Canthium exhibited greater inhibition of oxidation in microsomes (73%) than in the oil emulsion (21%). Heat treatment resulted in reduction of radical scavenging activity of extract from 68% to 40%. At pH 4.5 and 7 methanol extract exhibited some percent of antioxidant activity which ranged between 18 and 32%. Data indicates Canthium as a good source of antioxidants and methanol extract exhibited good antioxidant activity.

scholarly journals Antioxidant, Antidiabetic, and Anti-obesity Potential of Ipomoea reptans Poir Leaves

2020 ◽  
Vol 3 (4) ◽  
pp. 216-226
Author(s):  
Hendrik Kurniawan ◽  
Ermenilda Sonia Dacamis ◽  
Adelina Simamora ◽  
Priscilla Sari Dianauli Lumban Tobing ◽  
Ali Hanapiah ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Reducing Power ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Limited Information ◽  
Concentration Dependent Manner ◽  
Potent Inhibition ◽  
Total Antioxidant ◽  
Dpph Scavenging

Ipomoea reptans Poir or kangkung is a popular leafy vegetable, a favorite to people in Asian countries. However, limited information is available on their bioactivities. In the present study, the antioxidant, antidiabetic, and anti-obesity potential of I. reptans leaves were investigated. Different fractions (ethanol, ethyl acetate, and hexane) of I. reptans leaves were evaluated for their scavenging activity on DPPH radicals, whereas their reducing potential was investigated by cupric reducing antioxidant capacity (CuPRAC), total antioxidant, and reducing power assays. The antidiabetic potential was investigated by their inhibition effect on a-glucosidase. Total phenolic and flavonoid contents of I. reptans leaves were solvent dependent. Ethyl acetate contained the highest phenolic content, followed by ethanol and hexane fractions. However, for flavonoid content, the order was ethanol > ethyl acetate > hexane. All fractions showed DPPH scavenging activity in a concentration-dependent manner, with activities weaker than standards ascorbic acid and BHT, in the order of ethanol > ethyl acetate > hexane. All fractions showed reducing capacity, but only hexane and ethanol fractions of I. reptans leaves showed inhibition on a-glucosidase, with hexane showed more potent inhibition compared to acarbose. The study also found that fractions of I. reptans inhibit lipase and trypsin, enzymes related to lipid metabolism. Findings in this study offer a prospect for I. reptans leaves as a functional food source for antioxidant, antidiabetic, and anti-obesity purposes.

scholarly journals In vitro Evaluation of Antioxidant Potential of Leaves of Opuntia dillenii Haw.

1970 ◽  
Vol 2 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Raushanara Akter ◽  
SM Raquibul Hasan ◽  
Md Mokarram Hossain ◽  
Taksim Ahmed ◽  
Muntasir M Majumder ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Nitric Oxide Radical ◽  
Opuntia Dillenii

Antioxidant potential of the 80% methanol extract of the leaves of Opuntia dillenii was evaluated using 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) and nitric oxide radical scavenging, reducing power, total phenol and total flavonoid content determination assays. Preliminary phytochemical screening of the extract was also carried out, which revealed that the extract possesses flavonoids, steroids, alkaloids and tannins. The extract showed significant antioxidant activities in all antioxidant assays compared to the reference antioxidant in a dose dependent manner. In DPPH radical scavenging activity, the IC50 value of the crude extract was found to be 15.71μg/mL while the IC50 value for the reference ascorbic acid was 10.84 μg/mL. Again, the extract showed remarkable nitric oxide scavenging potential and good reducing power. Moreover, the methanol extract was found to contain high amount of phenols and flavonoids, expressed as gallic acid and rutin equivalents respectively. Based on the findings of the present study, we conclude that the methanol extract of the leaves of O. dillenii possesses remarkable antioxidant potential which may be attributed to the high amount of phenols and flavonoids present in the extract. Key Words: Opuntia dillenii; Antioxidant; Reactive oxygen species; DPPH; Nitric oxide radical DOI: 10.3329/sjps.v2i1.5811Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 22-26

Measurement of Total Phenolics, Antiradical Capacity and Reducing Power of Extracts of Chinese Medicinal Plants

2012 ◽  
Vol 217-219 ◽  
pp. 1002-1005
Author(s):  
Xin Hong Wang
Keyword(s):  
Functional Foods ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Antioxidant Ability ◽  
Ic50 Value ◽  
Chinese Medicinal Plants ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

P. cocos, B. ochracea, P. lactiflora, A. dahurica were procured and identified and seperately extracted using water (ethanol, methanol) in the ultrasonic cleaner. TPC, DPPH scavenging ability and reducing power (RP) were determined. The results from this study showed that P. lactiflora had the highest content as 18.62 mg GAE/g dw in ethanol, whereas P. cocos contained only 0.37 mg GAE/g dw in water and P. lactiflora exhibits the highest capacity (68.97%) to reduce DPPH in methanol extract with lowest IC50 value of 1.12 mg dw /ml and 1.14 mg dw /ml in ethanol extract at a concentration of 1.6 mg dw/ml. In RP assay, P. lactiflora had highest FRAP values (37.65 mg AAE/g dw in methanol extract), and P. cocos with least activity 3.25 mg AAE/g dw. The RP value has a apparently positive correlation with TPC and DPPH scavenging activity. TPC is remarkably related to its overall antioxidant ability. P. lactiflora could be selected as an excellent antioxidant for functional foods as well as pharmaceutics.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Choon Young Kim ◽  
Qi-Ming Yu ◽  
Hyun-Joo Kong ◽  
Joo-Yeon Lee ◽  
Kyung-Mi Yang ◽  
...  
Keyword(s):  
Cell Damage ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
Total Antioxidant ◽  
Anti Inflammatory ◽  
Species Production ◽  
Dose Dependent

The purpose of this study is to investigate the effect of Agrimonia pilosa Ledeb. extract (APLE) on lipopolysaccharide- (LPS-) induced cell damage in hepatocytes with a focus on antioxidant and anti-inflammatory activities. Total antioxidant and anti-inflammatory activities of APLE itself were analyzed and phytochemical analysis was performed. Moreover, inhibitory effects of APLE on LPS-induced oxidative stress and inflammation were assessed in human HepG2 hepatocytes. APLE was found to exert α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and nitrite scavenging activities and reducing power in a dose-dependent manner. The total phenolic and flavonoid contents of APLE were 44.30 ± 1.61 mg GAE/g and 29.65 ± 1.81 mg QE/g, respectively. HPLC analysis revealed that gallic acid is the major phenolic compound in APLE, followed by rutin, genistein, taxifolin, quercetin, luteolin, and apigenin, in descending order. Treatment of 100 and 200 μg/mL APLE significantly reduced LPS-stimulated intracellular reactive oxygen species production to the basal level without any cytotoxicity. Oppositely, APLE reversed LPS-suppressed expression of glutathione peroxidase gene and protein. Consistent with this result, APLE suppressed LPS-triggered expression of proinflammatory cytokine genes in a dose-dependent manner. These results reinforce the fact that the antioxidant and anti-inflammatory activity of APLE helps protect hepatocytes from LPS. Thus, APLE may be utilized as a bioactive ingredient in functional foods.

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