scholarly journals Antioxidant and cytotoxic activities of Ageratum conyzoides stems

2013 ◽  
Vol 2 (2) ◽  
pp. 33-37 ◽  
Author(s):  
Fatema Nasrin
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ageratum Conyzoides ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Total Antioxidant

Modern civilization is facing more than hundreds of disorders associated with free radicals and natural antioxidants from non-edible plants are gaining importance to fight these disorders. The intention of this report is to evaluate a well known medicinal weed Ageratum conyzoides stems for its antioxidant and cytotoxic Effects. Antioxidant potentiality of the crude methanolic extract of the Ageratum conyzoides (AC) stems was investigated on DPPH scavenging activity, reducing ability, total antioxidant capacity as well as total phenolic contents. Cytotoxic study was done by brine shrimp lethality bioassay and vincristin sulphate was used as standard. The total phenols and total antioxidant capacity of AC was found to be 38.125 ± 2.01mg/g equivalent of gallic acid and 333.37 ± 4.22mg/gm equivalent of ascorbic acid, respectively. The percentage (%) scavenging of DPPH free radical of the extract was found to be concentration dependent with IC50 value 46.01 ± 2.23µg/ml while IC50 value of standard ascorbic acid was found to be 29.56 ± 0.11?g/ml. The reducing power of AC was found to be concentration dependent. The cytotoxicity exhibited by AC was found promising with LC50 value 1.32?g/ml, comparing with the LC50 (0.689?g/ml) values of vincristin sulphate. The present investigation suggests that Ageratum conyzoides possesses remarkable antioxidant and cytotoxic property.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13195 International Current Pharmaceutical Journal 2013, 2(2): 33-37

scholarly journals Antioxidant Activity of Methanol Extract from Several Indonesian Green Teas

2018 ◽  
Vol 26 (1) ◽  
pp. 018
Author(s):  
Adelina Simamora ◽  
Michael Steven ◽  
Adit Widodo Santoso ◽  
Flora Rumiati ◽  
Kris Herawan Timotius
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Green Tea ◽  
Total Antioxidant Capacity ◽  
Health Effect ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Total Antioxidant

<p>As a common beverage, green tea is supposed to have beneficial health effect, such as antioxidant. At present, there are many green teas available in the market in Jakarta. Their quality, in their antioxidant activity, should be proved. This study aimed to investigate the antioxidant activity of extracts of green tea products commercially available in Jakarta. Four green tea samples from different factories (coded as A, B, C, and D) were selected and macerated using methanol. DPPH radical scavenging activity, reducing power ability and total antioxidant capacity were used to measure the antioxidant activity. The total phenolic content (TPC) was also determined. The studied green teas had varied TPC from 23.80  to  84.03 mG GAE/g extract, ranked as B &gt; D &gt; A &gt; C. All samples exhibited various but strong antioxidant activity by DPPH assay, even better than standards ascorbic acid and butylated hydroxytoluene (BHT). However, all extracts showed similar activities in their reducing power ability and total antioxidant capacity, with activities less than standards.<strong> </strong>These findings confirm that the quality of the Indonesian commercial green teas were heterogeneous both in TPC or DPPH scavenging capacity. But, there is an indication that they are good as an antioxidant containing beverage. </p>

scholarly journals The Total Antioxidant Capacity and the Total Phenolic Content of Rice Using Water as a Solvent

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
C. Priyanthi ◽  
R. Sivakanesan
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Condensed Tannin ◽  
Reducing Power ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Tannin Content ◽  
Rice Varieties ◽  
Total Antioxidant

Background. The present study evaluates the antioxidant properties of some Sri Lankan red rice varieties using water extracts. Methods. Water extracts of rice varieties Attakkari, Bg2907, and Bg407 were used in this study. The total antioxidant capacity was measured by ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and reducing power assays. The total phenolic content (TPC), total flavonoid content (TFC), monomeric anthocyanin, and condensed tannin contents were measured by Folin-Ciocalteu, aluminium chloride, pH differential, and vanillin assays, respectively. Results. It was observed that mean FRAP, DPPH, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content were in the range of 0.561 ± 0.113 to 0.695 ± 0.077   mmol / 100   g fresh weight (FW), 26.07 ± 3.08 to 53.66 ± 7.61   mg / mL   FW , 33.49 ± 4.105.14 to 40.81 ± 3.65   mg / mL , 0.676 ± 0.078 to 0.900 ± 0.057   mg tannic acid equivalent (TAE)/g, 5.36 ± 0.75 to 6.38 ± 0.82   mg   TAE / g   FW , 0.0202 ± 0.005 to 0.0292 ± 0.009   mg / g   FW , and 0.078 ± 0.015 to 0.104 ± 0.017   mg   TAE / g   FW , respectively. Significant differences were observed in DPPH, reducing power, and TPC among rice varieties ( p < 0.05 ). Rice variety Attakkari had the highest total antioxidant capacity (TAC), scavenging activity, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content followed by Bg2907 and Bg406. Conclusion. Total phenolic compounds, total flavonoid, and condensed tannin are the major antioxidants in all three varieties of rice while the monomeric anthocyanin is only a minor antioxidant.

scholarly journals Phenolic Profile, Antioxidant and Anti-Proliferative Activities of Methanolic Extracts from Asclepias linaria Cav. Leaves

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 54
Author(s):  
José Alejandro Sánchez-Gutiérrez ◽  
Dafné Moreno-Lorenzana ◽  
Dioselina Álvarez-Bernal ◽  
Jacobo Rodríguez-Campos ◽  
José Roberto Medina-Medrano
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Ultra Performance Liquid Chromatography ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Scavenging Activity ◽  
Phenolic Composition ◽  
Alternative Source ◽  
Methanolic Extracts ◽  
Total Antioxidant

Asclepias linaria Cav. (Apocynaceae) is a shrubby plant endemic of Mexico which has been used in traditional medicine. However, the bioactive potential of this plant remains unexplored. In this study, the phenolic composition, antioxidant, and cytotoxic activities of A. linaria leaves were determined. In order to estimate the phenolic composition of the leaves, the total phenolic, flavonoid, and condensed tannins contents were determined. Furthermore, the antioxidant activity was measured by the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] (ABTS•+) radicals and the total antioxidant capacity. The phenolic compounds identified in the A. linaria leaves by ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) include phenolic acids, such as p-coumaric and ferulic acid, as well as flavonoids, such as rutin and quercetin. The leaves’ extracts of A. linaria showed a high scavenging activity of DPPH• and ABTS•+ radicals (IC50 0.12 ± 0.001 and 0.51 ± 0.003 µg/mL, respectively), high total antioxidant capacity values (99.77 ± 4.32 mg of ascorbic acid equivalents/g of dry tissue), and had a cytotoxic effect against K562 and HL60 hematologic neoplasia cells lines, but no toxicity towards the normal mononuclear cell line was observed. These results highlight the potential of A. linaria and could be considered as a possible alternative source of anticancer compounds.

scholarly journals Evaluation of Analgesic and Antioxidant Potential of the Leaves of Curcuma alismatifolia Gagnep.

1970 ◽  
Vol 1 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Raushanara Akter ◽  
SM Raquibul Hasan ◽  
Samira Ayesha Siddiqua ◽  
Muntasir Mamun Majumder ◽  
Md Mokarram Hossain ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Dependent Manner ◽  
Writhing Test ◽  
Ic50 Value ◽  
Tail Immersion ◽  
Total Antioxidant ◽  
Dose Dependent

In the present study, the antioxidant and analgesic potential of the 80% methanol extract of the leaves of Curcuma alismatifolia Gangnep was evaluated. The extract was investigated for its antioxidant activity using lipid peroxidation, total antioxidant capacity and reducing power assays. The extract showed significant antioxidant activities in lipid peroxidation assay compared to the standard antioxidant in a dose dependent manner. In lipid peroxidation assay, the IC50 value was found to be 122.43μg/mL while the IC50 value for the reference ascorbic acid was 147.87μg/ml. Moreover, Curcuma alismatifolia extract showed strong total antioxidant capacity and good reducing power. The analgesic activity was evaluated for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test in mice respectively. The extract, at the dose of 250 and 500 mg/kg, produced a significant (p < 0.05-0.001) increase in pain threshold in tail immersion methods in a dose dependent manner. In acetic acid-induced writhing test the extract, at a dose of 500 mg/kg, showed a maximum of 60.5% inhibition (p < 0.001) of writhing reaction compared to the reference drug diclofenac-sodium (75.0%). All experimental results suggest the use of this plant for the treatment of pain and inflammatory disorder. Key Words: Curcuma alismatifolia, Antioxidant, Lipid peroxidation, Total antioxidant capacity, Reducing power, Analgesic, Tail immersion. doi:10.3329/sjps.v1i1.1779 S. J. Pharm. Sci. 1(1&2): 3-9

scholarly journals Anti-inflammatory and Antioxidant Activities of Cucumis sativus Leaves

2015 ◽  
Vol 18 (2) ◽  
pp. 169-173
Author(s):  
Fatema Nasrin ◽  
Israt Jahan Bulbul ◽  
Fahima Aktar ◽  
Mohammad A Rashid
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Antioxidant Capacity ◽  
Cucumis Sativus ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Paw Edema ◽  
Total Antioxidant ◽  
Anti Inflammatory

This study was carried out to evaluate the anti-inflammatory and antioxidant activities of Cucumis sativus Linn. (Family: Cucurbitaceae) leaves. The methanolic extract of C. sativus leaves (MCS) was investigated for antiinflammatory activities in Long Evans rat model at two different doses of 150 and 250 mg/kg body weight and the effects were compared with the standard, indomethacin (10 mg/kg body weight). It exhibited highest antiinflammatory activity at the dose 250 mg/kg. In the formalin test, the extract at both doses (150 and 250 mg/kg body weight) significantly prevented the increase in volume of paw edema (P<0.05 and P<0.001). In carrageenan-induced paw edema test the MCS significantly (P?0.001) reduced inflammation by 57.35 % (150 mg/kg body weight) and 72.06% (250 mg/kg body weight) in comparison to the standard drug, indomethacin (79.41%) at the end of 5h. MCS was also screened for DPPH scavenging activity, total antioxidant capacity, reducing ability as well as total phenolics content to assess its antioxidant potential. Total phenolic content and total antioxidant capacity of MCS were found to be 262.31 mg/g equivalent of gallic acid and 267.2 mg/g equivalent of ascorbic acid, respectively. The IC50 of free radical scavenging of DPPH was 13.06 ?g/ml while that of standard ascorbic acid was 13.17 ?g/ml. The reducing power of MCS was found to be concentration dependent.Bangladesh Pharmaceutical Journal 18(2): 169-173, 2015

scholarly journals Moroccan Monofloral Bee Pollen: Botanical Origin, Physicochemical Characterization, and Antioxidant Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
El Ghouizi Asmae ◽  
El Menyiy Nawal ◽  
Meryem Bakour ◽  
Badiaa Lyoussi
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Physicochemical Characterization ◽  
Reducing Power ◽  
Total Phenolic ◽  
Mineral Contents ◽  
Bee Pollen ◽  
Two Samples ◽  
Total Antioxidant

In this study, eight monofloral bee pollen samples were collected from different apiaries in Morocco. Botanical origins of the bee pollen samples were determined by scanning electron microscopy (SEM), and the physicochemical parameters (pH, moisture, ash, and the mineral contents) were determined. Total phenolic, flavones/flavonols contents were evaluated, and the antioxidant potential was assessed using total antioxidant capacity, DPPH, ABTS, and reducing power assays. Data showed that pH, moisture, and ash content values ranged between 4.19 ± 0.17 and 4.82 ± 0.36, 10.7 ± 0.04% and 26.8 ± 0.01%, and 1.81 ± 0.10% and 4.22 ± 0.08%, respectively. Potassium and magnesium were the most abundant minerals in bee pollen samples; heavy metals were not detected except for two samples (P5 and P6) where a very small amount of lead was found. The protein content in these samples varied between 19.86 ± 0.36 mg/100 g and 30.32 ± 0.12 mg/100 g of bee pollen. The phenolic content, flavones/flavonols content, and total antioxidant capacity were 21.87 ± 1.80 mgEAA/g, 2.37 ± 0.16 mgEAA/g, and 6.23 ± 0.21 mgEAA/g, respectively. High scavenging activity of DPPH and ABTS radicals was found in P2 with the lower IC50 of 0.245 ± 0.009 mg/ml and 0.19 ± 0.005 mg/ml, respectively. The lower EC50 was 0.133 ± 0.036 mg/ml found in P1 for the reducing power test. The current study is considered to be the first step to the standardization of Moroccan bee pollen.

scholarly journals A Comparison of Antioxidant Properties of Two Apple Cultivars

2019 ◽  
Vol 68 (3-4) ◽  
pp. 60-64 ◽  
Author(s):  
Emir Šahinović ◽  
Senad Murtić
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Fresh Weight ◽  
Total Antioxidant ◽  
Apple Cultivars ◽  
Apple Fruits

Summary The apple (Malus domestica Borkh.) possesses a number of health-promoting properties. The main beneficial effects of apple fruits on human health are associated with the antioxidant properties of components synthesising in apple fruits during their growth and development. Phenolic compounds and flavonoids are such components that contribute significantly to the antioxidant capacity of fruits, thus enhancing their health properties. The purpose of this study is to compare the antioxidant properties (namely antioxidant capacity, ascorbic acid, total phenolic and flavonoid contents) of the ‘Idared’ and ‘Braeburn’ apple cultivars, grown under optimal growing conditions in the Goražde area. Ascorbic acid contents were estimated using the 2.6-dichlorophenolindophenol titration method, whereas total phenolics were determined using the Folin-Ciocalteu method. Furthermore, total flavonoids were determined using the aluminium chloride colorimetric assay, whereas the ferric reducing/antioxidant power assay was used to determine the total antioxidant capacity of the apple fruits examined. The average values of the ascorbic acid, the total phenolic and flavonoids contents, and the total antioxidant capacity of the ‘Idared’ apples under consideration were 8.09 mg 100 g−1, 34.56 mg gallic acid equivalents 100 g−1, 12.62 mg catechin equivalents 100 g−1 and 323.28 μmol Fe2+ 100 g−1of fresh weight, respectively. The average values of the total phenolic and flavonoids contents, as well as the antioxidant capacity of the ‘Braeburn’ apples examined were as follows: 8.89 mg 100 g−1, 33.4 mg gallic acid equivalents 100 g−1, 13.56 mg catechin equivalents 100 g−1 and 367.01 μmol Fe2+ 100 g−1 of fresh weight, respectively. The analyses performed indicate that the differences recorded in the parameter values between the apple cultivars ‘Idared’ and ‘Braeburn’ were statistically non-significant. A correlation data analysis confirmed that phenolic compounds are the carriers of the antioxidant activity of apples.

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

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