scholarly journals Evaluation of Analgesic and Antioxidant Potential of the Leaves of Curcuma alismatifolia Gagnep.

1970 ◽  
Vol 1 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Raushanara Akter ◽  
SM Raquibul Hasan ◽  
Samira Ayesha Siddiqua ◽  
Muntasir Mamun Majumder ◽  
Md Mokarram Hossain ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Dependent Manner ◽  
Writhing Test ◽  
Ic50 Value ◽  
Tail Immersion ◽  
Total Antioxidant ◽  
Dose Dependent

In the present study, the antioxidant and analgesic potential of the 80% methanol extract of the leaves of Curcuma alismatifolia Gangnep was evaluated. The extract was investigated for its antioxidant activity using lipid peroxidation, total antioxidant capacity and reducing power assays. The extract showed significant antioxidant activities in lipid peroxidation assay compared to the standard antioxidant in a dose dependent manner. In lipid peroxidation assay, the IC50 value was found to be 122.43μg/mL while the IC50 value for the reference ascorbic acid was 147.87μg/ml. Moreover, Curcuma alismatifolia extract showed strong total antioxidant capacity and good reducing power. The analgesic activity was evaluated for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test in mice respectively. The extract, at the dose of 250 and 500 mg/kg, produced a significant (p < 0.05-0.001) increase in pain threshold in tail immersion methods in a dose dependent manner. In acetic acid-induced writhing test the extract, at a dose of 500 mg/kg, showed a maximum of 60.5% inhibition (p < 0.001) of writhing reaction compared to the reference drug diclofenac-sodium (75.0%). All experimental results suggest the use of this plant for the treatment of pain and inflammatory disorder. Key Words: Curcuma alismatifolia, Antioxidant, Lipid peroxidation, Total antioxidant capacity, Reducing power, Analgesic, Tail immersion. doi:10.3329/sjps.v1i1.1779 S. J. Pharm. Sci. 1(1&2): 3-9

scholarly journals Antioxidant and cytotoxic activities of Ageratum conyzoides stems

2013 ◽  
Vol 2 (2) ◽  
pp. 33-37 ◽  
Author(s):  
Fatema Nasrin
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ageratum Conyzoides ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Total Antioxidant

Modern civilization is facing more than hundreds of disorders associated with free radicals and natural antioxidants from non-edible plants are gaining importance to fight these disorders. The intention of this report is to evaluate a well known medicinal weed Ageratum conyzoides stems for its antioxidant and cytotoxic Effects. Antioxidant potentiality of the crude methanolic extract of the Ageratum conyzoides (AC) stems was investigated on DPPH scavenging activity, reducing ability, total antioxidant capacity as well as total phenolic contents. Cytotoxic study was done by brine shrimp lethality bioassay and vincristin sulphate was used as standard. The total phenols and total antioxidant capacity of AC was found to be 38.125 ± 2.01mg/g equivalent of gallic acid and 333.37 ± 4.22mg/gm equivalent of ascorbic acid, respectively. The percentage (%) scavenging of DPPH free radical of the extract was found to be concentration dependent with IC50 value 46.01 ± 2.23µg/ml while IC50 value of standard ascorbic acid was found to be 29.56 ± 0.11?g/ml. The reducing power of AC was found to be concentration dependent. The cytotoxicity exhibited by AC was found promising with LC50 value 1.32?g/ml, comparing with the LC50 (0.689?g/ml) values of vincristin sulphate. The present investigation suggests that Ageratum conyzoides possesses remarkable antioxidant and cytotoxic property.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13195 International Current Pharmaceutical Journal 2013, 2(2): 33-37

scholarly journals In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi Linn.

1970 ◽  
Vol 6 (2) ◽  
pp. 197-202 ◽  
Author(s):  
MR Saha ◽  
SMR Hasan ◽  
R Akter ◽  
MM Hossain ◽  
MS Alam ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Reducing Power ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Mimusops Elengi ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay ◽  
Dose Dependent

In the present study, antioxidant potential of the methanol extract of the leaves of Mimusops elengi Linn. was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activities in all antioxidant assays compared to the reference antioxidant ascorbic acid in a dose dependent manner. In DPPH scavenging assay the IC50 value of the extract was found to be 43.26μg/ml while the IC50 value of the reference standard ascorbic acid was 58.92 μg/ml. Total antioxidant activity was also found to increase in a dose dependent manner. Moreover, M. elengi extract showed strong reducing power. These results suggest that Mimusops elengi may act as a chemopreventative agent, providing antioxidant properties and offering effective protection from free radicals. Key words: Mimusops elengi, antioxidant, reducing power, total antioxidant capacity, reactive oxygen species   doi: 10.3329/bjvm.v6i2.2336 Bangl. J. Vet. Med. (2008). 6 (2): 197-202

scholarly journals Antioxidant, cytotoxic and analgesic activities of the methanolic fruit extract of Terminalia chebula Retz.

2013 ◽  
Vol 3 (1) ◽  
pp. 219-222
Author(s):  
Shammy Sarwar ◽  
Asif Hasan Malik ◽  
Muhammad Ashikur Rahman ◽  
Md. Zakiur Rahman ◽  
Md. Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Ascorbic Acid ◽  
Cytotoxic Activity ◽  
Reducing Power ◽  
Dependent Manner ◽  
Terminalia Chebula ◽  
Reference Drug ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
Dose Dependent

The present study was aimed to investigate antioxidant, analgesic and cytotoxic activity of the methanolic extract of Terminalia chebula Retz. fruits. Antioxidant potential of the extract was evaluated by using nitric oxide scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activities in all antioxidant assays compared to ascorbic acid in a dose dependent mode. In nitric oxide scavenging assay, the IC50 value of the extract was found to be 51.3 µg/mL while the IC50 value of ascorbic acid was 77.4 µg/mL. In addition to strong reducing power, total antioxidant activity of the extract was also found to increase in a dose dependent manner. The analgesic activity was evaluated using acetic acid-induced writhing test in mice. The extract, at a dose of 500 mg/kg, showed a maximum of 44.17 % inhibition (p < 0.05) of writhing reaction compared to the reference drug diclofenac-sodium (66.96 %). The extract also showed moderate cytotoxic activity in brine shrimp lethality bioassay and the LC50 value was found to be 97.36 µg/mL.DOI: http://dx.doi.org/10.3329/icpj.v3i1.17296 International Current Pharmaceutical Journal, December 2013, 3(1): 219-222

scholarly journals Enhancement of polyphenols and antioxidant and inhibitory properties of Corchorus olitorius and Amaranthus hybridus leaves by the Ultraviolet-C treatment

2020 ◽  
Vol 12 (2) ◽  
pp. 203-210
Author(s):  
Kayode Olayele Karigidi ◽  
Olufunke Omowunmi Adebogun
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Phenolics ◽  
Reducing Power ◽  
Total Flavonoids ◽  
Corchorus Olitorius ◽  
Amaranthus Hybridus ◽  
Total Antioxidant ◽  
Ultraviolet C

The effect of the postharvest (Ultraviolet-C) treatment on total phenolics, total flavonoids, total antioxidant capacity, 1,1-diphenyl-2-picrylhydrazyl scavenging activity(DPPH),and reducing power of Corchorus olitorius(CO) and Amaranthus hybridus(AH)was investigated. Their abilities to inhibit induced lipid peroxidation, α-amylase,and α-glucosidase after the ultraviolet-C treatment were also evaluated. The leaves were exposed to ultraviolet-C radiation for 10 min. Total phenolics, total flavonoids, total antioxidant capacity, DPPH scavenging ability,and reducing power were significantly (p &lt; 0.05) increased after the ultraviolet-C treatment. Also,their abilities to inhibit SNP-induced lipid peroxidation, α-amylase and α-glucosidase were also enhanced significantly (p &lt; 0.05). Therefore,apart from using ultraviolet-C radiation in water purification, it can be used to enhance health-promoting components in fruit and vegetables.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

Studies on the Antioxidant Activities of Total Alkaloids from the Fruits of Evodia rutaecarpa (Juss.) Benth

2011 ◽  
Vol 396-398 ◽  
pp. 52-55 ◽  
Author(s):  
Ming Xiong Tan ◽  
Yan Cheng Liu ◽  
Xu Jian Luo ◽  
Dong Qing Li
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Dpph Radical ◽  
Synthetic Antioxidant ◽  
Total Alkaloids ◽  
Evodia Rutaecarpa ◽  
Total Antioxidant

The total alkaloids (TAE) were obtained from the fruits of Evodia rutaecarpa (Juss.) Benth. The antioxidant activities of TAE towards the inhibitory effect on 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical, total antioxidant capacity, and inhibition on lipid peroxidation were investigated. TAE was found that the total antioxidant capacity and inhibitory lipid peroxidation are superior to synthetic antioxidant 2, 6-di-ter-butyl-4-methylphenol (BHT), but scavenging activity on DPPH radical is lower than that of BHT at the same condition.

scholarly journals Environmental enrichment and exercise are better than social enrichment to reduce memory deficits in amyloid beta neurotoxicity

2018 ◽  
Vol 115 (10) ◽  
pp. E2403-E2409 ◽  
Author(s):  
Mariza G. Prado Lima ◽  
Helen L. Schimidt ◽  
Alexandre Garcia ◽  
Letícia R. Daré ◽  
Felipe P. Carpes ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Physical Exercise ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Environmental Enrichment ◽  
Memory Deficits ◽  
Neuroprotective Effects ◽  
Antioxidant Power ◽  
Term Memory ◽  
Total Antioxidant

Recently, nongenetic animal models to study the onset and development of Alzheimer’s disease (AD) have appeared, such as the intrahippocampal infusion of peptides present in Alzheimer amyloid plaques [i.e., amyloid-β (Aβ)]. Nonpharmacological approaches to AD treatment also have been advanced recently, which involve combinations of behavioral interventions whose specific effects are often difficult to determine. Here we isolate the neuroprotective effects of three of these interventions—environmental enrichment (EE), anaerobic physical exercise (AnPE), and social enrichment (SE)—on Aβ-induced oxidative stress and on impairments in learning and memory induced by Aβ. Wistar rats were submitted to 8 wk of EE, AnPE, or SE, followed by Aβ infusion in the dorsal hippocampus. Short-term memory (STM) and long-term memory (LTM) of object recognition (OR) and social recognition (SR) were evaluated. Biochemical assays determined hippocampal oxidative status: reactive oxygen species, lipid peroxidation by thiobarbituric acid reactive substance (TBARS) test, and total antioxidant capacity by ferric reducing/antioxidant power (FRAP), as well as acetylcholinesterase activity. Aβ infusion resulted in memory deficits and hippocampal oxidative damage. EE and AnPE prevented all memory deficits (STM and LTM of OR and SR) and lipid peroxidation (i.e., TBARS). SE prevented only the SR memory deficits and the decrease of total antioxidant capacity decrease (i.e., FRAP). Traditionally, findings obtained with EE protocols do not allow discrimination of the roles of the three individual factors involved. Here we demonstrate that EE and physical exercise have better neuroprotective effects than SE in memory deficits related to Aβ neurotoxicity in the AD model tested.

scholarly journals Serum total antioxidant capacity prior to liver transplantation for hepatocellular carcinoma is associated with 1-year liver transplantation survival

2018 ◽  
Vol 46 (7) ◽  
pp. 2641-2649 ◽  
Author(s):  
Leonardo Lorente ◽  
Sergio T. Rodriguez ◽  
Pablo Sanz ◽  
Antonia Pérez-Cejas ◽  
Pedro Abreu-González ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Lipid Peroxidation ◽  
Liver Transplantation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Serum Levels ◽  
Study Endpoint ◽  
First Year ◽  
Inverse Association ◽  
Total Antioxidant

Objective To determine whether there was an association between serum total antioxidant capacity (TAC) levels prior to in liver transplantation (LT) for hepatocellular carcinoma (HCC) and 1-year LT mortality. Methods This observational retrospective single-centre study of patients with LT for HCC measured serum levels of TAC and malondialdehyde (as a biomarker of lipid peroxidation) before LT. The study endpoint was 1-year LT mortality. Results This study included 142 patients who underwent LT for HCC. Patients who survived the first year ( n = 127) had significantly lower aged liver donors, significantly higher serum TAC levels, and significantly lower serum malondialdehyde levels compared with the non-survivors ( n = 15). Logistic regression analysis found that serum TAC levels (odds ratio [OR] 0.275; 95% confidence interval [CI] 0.135, 0.562) and the age of the LT donor (OR 1.050; 95% CI 1.009, 1.094) were associated with 1-year LT mortality. There was an inverse association between serum levels of TAC and malondialdehyde levels (rho = –0.22). Conclusions There was an association between low serum TAC levels prior to LT for HCC and mortality during the first year after LT. There was an inverse association between serum TAC levels and lipid peroxidation as measured by malondialdehyde levels.

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