scholarly journals CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

2012 ◽  
Vol 9 (1) ◽  
pp. 67-71 ◽  
Author(s):  
MA Momin ◽  
MA Islam ◽  
MM Khatun ◽  
MM Rahman ◽  
MA Islam
Keyword(s):  
Antimicrobial Agents ◽  
Research Work ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Highly Sensitive ◽  
Golden Yellow ◽  
Agar Media ◽  
Staphylococcus Spp

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71 

Isolation and Identification of Staphylococcus Aureus from Dairy Farms in Bishoftu Town, Ethiopia

2021 ◽  
Author(s):  
Sara Amanuel Bude ◽  
Abdi Kidane Mengesha
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Farms ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Swab Sample ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Mannitol Salt Agar ◽  
Milk Samples ◽  
Golden Yellow

Abstract A cross-sectional study was conducted from February, 2020 to March, 2020 in selected dairy farms in Bishoftu Town to isolate and identify Staphylococcus aureus from milk samples, swab (teat swab, tank swab and bucket swab) samples. The samples were transported to microbiology laboratory and, isolation and identification of an organism was based on morphological, cultural and biochemical characteristics. Accordingly a total of 120 samples of them 58 milk sample, 58 polled teat swab sample, 2 polled tank swab sample and 2 polled bucket swab samples. Those milk samples and swab sample were collected and cultured parallel on both Blood agar and nutrient agar. Out of total samples 68/120 were well grown on both Medias and sub cultured on nutrient agar for farther identification using Primary and secondary biochemical tests. Accordingly, 68 milk samples were shown typical large, round, golden yellow colonies with hemolysis when grown on blood agar, round shaped grape like clusters are seen under microscope after stained with grams stain, and catalase positive Staphylococcus aureus with bubble formation is observed and typical yellow pigmentation on mannitol salt agar which is selective media for genus Staphylococcus. Finally, 68(56.67%) Staphylococcus aureus were identified with coagulase test with the clumping result observed. Hence, implementing hygiene conditions, creation of awareness on control and prevention of subclinical mastitis in dairy farms and conducting drug sensitivity test for Staphylococcus aureus is recommended.

scholarly journals Antimicrobial Studies and Characterization of Copper Surfactants Derived from Various Oils Treated at High Temperatures by P.D.A. Technique

2018 ◽  
Vol 5 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Renu Bhutra ◽  
Rashmi Sharma ◽  
Arun Kumar Sharma
Keyword(s):  
Antimicrobial Agents ◽  
Edible Oils ◽  
Research Work ◽  
Fungal Species ◽  
Spectroscopic Technique ◽  
Antimicrobial Studies ◽  
Free Environment ◽  
Copper Soap ◽  
Ir Spectroscopic

Introduction:Biologically potent compounds are one of the most important classes of materials for the upcoming generations. Increasing number of microbial infectious diseases and resistant pathogens create a demand and urgency to develop novel, potent, safe and improved variety of antimicrobial agents. This initiates a task for current chemistry to synthesize compounds that show promising activity as therapeutic agents with lower toxicity. Therefore, a substantial research is needed for their discovery and improvement. Chemistry of present era aims to build a pollution free environment. For the same, it targets to create some alternativeswhich are eco-friendly and nature loving. Present research work is a step towards achieving such alternatives.Method:For this the metallic soaps of copper (derived from common edible oils) were synthesized. The synthesized copper soaps have been confirmed by elemental analysis, UV, and IR spectroscopic technique. The fungicidal activities of copper soaps derived from soyabean, sesame oils have been evaluated by testing against Alternaria alternate and Aspergillus niger by P.D.A. technique.Result:The fungi toxicity results indicate that the strain of fungal species are susceptible towards these soaps and suggests that with the increase in concentration of copper soap it may increase further. The transition metallic soaps showed good antifungal activity because chelation increases the anti-microbial potency.

scholarly journals Use of Amino Oligosaccharins and Alternaria Fine Protein in the Management of Crown Gall Disease in Roses

2020 ◽  
Vol 8 (2) ◽  
pp. 118-127
Author(s):  
Emmanuel Wesonga Atsango ◽  
William Maina Muiru ◽  
Agnes Wakesho Mwang’ombe ◽  
Liu Gaoqiong
Keyword(s):  
Crown Gall ◽  
Block Design ◽  
Foliar Spray ◽  
Negative Control ◽  
Rosa Hybrida ◽  
Nutrient Agar ◽  
Biochemical Tests ◽  
Crown Gall Disease ◽  
Pests And Diseases ◽  
Agar Media

Rose (Rosa hybrida Vill.) production is limited by a variety of factors such as poor mineral nutrition, high salinity, pests and diseases. Crown gall disease caused by Agrobacterium tumefaciens causes a significant damage to roses in Kenya. The study was carried out in Winchester farm (Nairobi) and Bahati farm (Nakuru) to determine the effects of a mixture of amino oligosaccharins and Alternaria fine proteins on crown gall disease in roses. The experiment was conducted on Rosa hybrida var. Mariyo in a Randomized Complete Block Design with four replications. The treatments comprised of different rates 0.5, 1, 1.5g/L of water of the product at 3% concentration applied as foliar spray and a commonly used product copper sulphate pentahydrate (Mastercop) produced by ADAMA applied at 2ml/ L as the standard and water as a negative control. Crown gall tissues were collected from four different roses per treatment from the two sites, counted and used for biochemical tests in Kabete laboratory. Bacteria were isolated by culturing and the representative colony types growing on nutrient agar media selected and sub-cultured by successive streaking on nutrient agar media. The biochemical test for the different isolates was done to identify the bacterial isolates. Application of the mixture of amino oligosaccharins and Alternaria activated protein at the rate of 1.5g/L had significant effects on galls formation and reduced the numbers significantly at both farms. The gram reaction indicated that the selected isolates were gram negative and were positive for motility, catalase, oxidase, lactose, mannitol, and salt tolerance tests. There was a significant reduction in the number of galls and size following the application of amino oligosaccharins and Alternaria fine proteins as well as improvement in plant growth. The ability to manage the disease can be attributed to enhanced defense enzyme activity enhanced by amino oligosaccharins and Alternaria fine proteins.

Studies on Trypanosoma avium

Parasitology ◽  
1966 ◽  
Vol 56 (1) ◽  
pp. 15-19 ◽  
Author(s):  
J. R. Baker
Keyword(s):  
Nutrient Agar ◽  
Blood Agar ◽  
Insect Vector ◽  
Corvus Frugilegus ◽  
Agar Media

A strain of Trypanosoma avium isolated from Corvus frugilegus in England was readily cultivated in vitro in diphasic blood agar, or blood nutrient agar, media at 28 °C.In both media, small trypanosomes appeared which morphologically resembled the metacyclic forms from the insect vector and which were infective to canaries. They were more numerous, and developed more regularly, in the medium with the nutrient agar base.At 40–41 °C a few of these small trypanosomes apparently developed into large trypanosomes similar to, but not identical with, those seen in the blood of infected birds.

scholarly journals IDENTIFIKASI BAKTERI AEROB DI UDARA RUANG OPERASI INSTALASI BEDAH SENTRAL (IBS) RSUP ROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Billy V. Palawe ◽  
Constantien Kountul ◽  
Olivia Waworuntu
Keyword(s):  
Bacillus Subtilis ◽  
Operating Room ◽  
Descriptive Study ◽  
Aspergillus Species ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Aerobic Bacteria ◽  
Particulate Matters ◽  
Water Droplets ◽  
Agar Media

Abstract: Air is not a medium where microbes grow, but it is a carrier of particulate matters, such as dust and water droplets which are very probably loaded with microbes. And the most of bacteria that live in the air of the hospitals room environment consist of Bacillus bacteria, E.coli, Staphylococcus, Streptococcus, Pseudemonas, also fungi which is Aspergillus species. Objective: To determine the existence of aerobic bacteria and others species in the operating room of air Installation Central Surgery (IBS). Prof. Dr. R. D. Kandou Manado. Methods: It is a prospective descriptive study to get an overview of the bacteria in the air of aerobic Central Surgery Installation operating room (IBS) Prof. Dr. R. D. Kandou Manado. Results: Staphylococcus Albus bacteria found in Nutrient Agar media (NA) And Blood Agar (AD), while Bacillus subtilis found in Blood Agar media (AD).Conclusion: In the air of Central Surgery Installation operations room (IBS) Prof. Dr. RD Kandou Manado, based on the result shows the high number of aerobic bacteria.Keywords: aerobic bacteria,in the operations room air.Abstrak: Udara bukan merupakan medium tempat mikroba tumbuh, tetapi merupakan pembawa bahan partikulat, debu, dan tetesan air yang semuanya sangat mungkin dimuati mikroba. Mikroorganisme yang paling banyak berkeliaran di udara lingkungan rumah sakit adalah bakteri Bacillus, E.coli, Staphylococcus, Streptococcus, Pseudemonas, dan spesies jamur seperti Aspergillus. Tujuan: Mengetahui adanya bakteri aerob dan jenis lainnya di udara ruang operasi Instalasi Bedah Sentral (IBS) RSUP. Prof. Dr. R. D. Kandou Manado. Metode: Penelitian ini bersifat penelitian deskriptif prospektif yaitu untuk mendapatkan gambaran bakteri aeorob di udara ruang operasi Instalasi Bedah Sentral (IBS) RSUP Prof. Dr. R. D. Kandou Manado. Hasil: Staphylococcus Albus ditemukan di media spesifik Agar Nutrientt (NA) dan Agar Darah (AD) sedangkan Bacillus Subtilis ditemukan di media spesifik Agar Darah (AD). Simpulan: Udara diruang operasi Instalasi Bedah Sentral (IBS) RSUP Prof. Dr.R. D. Kandou Manado, menunjukan masih tingginya jumlah angka bakteri aerob.Kata kunci: bakteri aerob, udara diruang operasi.

scholarly journals Microbial Quality of Raw Beef and Chevon From Selected Markets in Cape Coast, Ghana

2019 ◽  
Vol 10 (1) ◽  
pp. 78 ◽  
Author(s):  
Levi Yafetto ◽  
Emelia Hornam Adator ◽  
Abena Ahema Ebuako ◽  
Ephraim Ekloh ◽  
Francis Yao Afeti
Keyword(s):  
Nutrient Agar ◽  
Microbial Quality ◽  
Macconkey Agar ◽  
Cape Coast ◽  
Salmonella Spp ◽  
Bacterial Counts ◽  
Agar Media ◽  
Stock Solutions ◽  
Staphylococcus Spp

This study assessed microbial quality of raw beef and chevon (goat meat) from selected meat retail shops in Abura, Kotokuraba and Science markets in Cape Coast, Ghana. Stock solutions from beef and chevon were analyzed on nutrient agar, MacConkey agar, and potato dextrose agar media using microbiological procedures. Results revealed that beef from Kotokuraba market was the most contaminated with mean highest bacterial counts of 1.15x108 and 9.40x107 cfu/ml in nutrient agar and MacConkey agar media, respectively. The results further showed that chevon from Science market was the most contaminated with mean highest bacterial counts of 1.67x108 and 7.10x107 cfu/ml in nutrient agar and MacConkey agar media, respectively. Mean fungal counts in PDA medium was the least recorded for both beef and chevon from all the three markets. Comparative analyses of results suggest that chevon was more contaminated than beef from Abura market, whereas beef was more contaminated than chevon from Kotokuraba market. However, from Science market, except in MacConkey agar medium, where beef was more contaminated than chevon, chevon was more contaminated than beef in nutrient agar and PDA media. Bacteria isolated were Escherichia coli, Klebsiella spp., Nocardia spp., Salmonella spp., Staphylococcus spp., and Streptococcus spp. Fungi of the genera Aspergillus, Candida, Fusarium, Penicillium, and Rhodotorula were isolated. We conclude that raw beef and chevon sold in markets in Cape Coast are contaminated by pathogenic and toxigenic microbes that may affect meat quality and consequently pose public health concerns to consumers.

scholarly journals Identification of pathogenic bacteria from infected Thai koi (Anabas testudineus)

2019 ◽  
Vol 5 (1) ◽  
pp. 56-62
Author(s):  
Mousumi Sarker Chhanda ◽  
Imran Parvez ◽  
Nazmi Ara Rumi ◽  
Md Hafiz All Hosen ◽  
Md Rezaul Islam
Keyword(s):  
Experimental Infection ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Anabas Testudineus ◽  
Mannitol Salt Agar ◽  
Fresh Fish ◽  
Agar Media ◽  
Staphylococcus Spp

A study was conducted for identification of pathogenic bacteria from Thai koi (Anabas testudineus) and an experimental infection test was run for identifying the actual causative agent of the infection. Due to perform the experiment, the fish sample was collected from different fish farm located in Fulbari, Dinajpur to the Microbiology lab of the HSTU-Dnajpur campus and placed in tray for taking sample from different infected part such as gill, slime, muscle, fin of the fish by using wire loop then these were taking in the nutrient ager medium for observing the culture of bacteria. After then specific culture media, Salmonella-Shigella media, Mannitol salt agar media, Mac-Conkey (MaC) agar media and Eosin Methylene Blue media were used for observing specific bacterial characteristics. Then biochemical tests, Methyl red (MR), Voges-proskaure test, Triple sugar iron test, Indole test were performed for bacterial identification. As a result Salmonella spp., Shigella spp., Klebshiella spp. and Staphylococcus spp. were confirmed. Catalase test and Simon citrate test was also performed. Then Grams staining method was followed for microscopic observation of identified bacteria. Then experimental infection test was performed in Aquaculture lab by setting up 5 aquarium holding fresh fish. The fresh water and identified bacteria were added specifically to the aquarium and it was continued for 15 days for observing infectious symptoms. After 15 days the fish with Salmonella spp. and Staphylococcus spp. were showed infectious symptoms but other did not any change in physical appearance. So it can be said that Staphylococcus spp. and Salmonella spp. are able to show ulcerative symptoms in Thai koi (Anabas testudineus) that is a bacterial infection. Asian J. Med. Biol. Res. March 2019, 5(1): 56-62

scholarly journals Detection of the main bacterial causative agents associated with pneumonia cases of pigeons in Al-Diwaniya province

2012 ◽  
Vol 11 (1) ◽  
pp. 144
Author(s):  
M.A. Khudair
Keyword(s):  
Blood Agar ◽  
Aerobic Bacteria ◽  
Biochemical Tests ◽  
Indirect Methods ◽  
E Coli ◽  
Escherchia Coli ◽  
Causative Agents ◽  
Identification Of Bacteria ◽  
Staphylococcus Spp ◽  
Direct And Indirect Methods

In this study, infected lung samples of pigeons at Al-Diwaniya province were tested for the presence of some aerobic bacteria. The identification of bacteria were proved by swabs culturing and biochemical tests. A total of 100 pigeons were examined at post-mortem, and 100 samples with pneumonia were collected. All lungs were collected from different local pigeon’s market showing respiratory disorders. Blood agar supplemented with 7% sheep blood was used for isolation of the agents.Two methods were used for culturing the samples collecting from lung which include direct and indirect methods. In both methods bacterial colonies were identified on blood agar and MacConkey agar after that differential media was used for bacterial purification; various biochemical tests were used to study the properties of each bacterium. The aim of this work was to isolate and identify the aerobic bacteria associated with cases showing typical signs of respiratory infections.Bacteriological result showed (107) bacterial isolates which include Escherchia coli represent high percentage (29.9%) and ,Staphylococcus spp.(14.%) were considered the most predominant bacteria in comparison to other isolated bacteria which include Streptococcus pneumonia, K. pneumonia, E. aerogenense and P. vulgaris. This study showed that E. coli is the most prevalent bacterial infection among pigeon population in Al-Diwaniya province.

scholarly journals Chromogenic Agar Medium : A Versatile Tool for the Diagnosis of UTI

2018 ◽  
Vol 25 ◽  
pp. 64-71
Author(s):  
S Gul Nahar ◽  
M Bulbul Hasan ◽  
Mst Rokeya Khatun ◽  
M Nawshad Ali ◽  
DK Mohanta
Keyword(s):  
Urine Culture ◽  
Blood Agar ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
College Hospital ◽  
Medical College ◽  
Presumptive Identification ◽  
Versatile Tool ◽  
Chromogenic Agar ◽  
Agar Media

Objective: The present study was done on Chromogenic agar media to identify uropathogens more efficiently by its characteristic colony colour for each of the organism.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto Chromogenic agar media, Blood agar and MacConkey agar media.Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both Chromogenic agar media and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on Chromogenic agar media when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on Chromogenic agar media in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on Chromogenic agar media, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: Chromogenic agar media has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2012; 25: 64-71

scholarly journals Antibiogram Analysis and Characterization of Bacterial Pathogens from Leucorrhea Patients

2019 ◽  
pp. 1-13
Author(s):  
Nazish Mazhar Ali ◽  
Muhammad Shahzad ◽  
Muqadas Bano ◽  
Iram Liaqat ◽  
Bushra Mazhar ◽  
...  
Keyword(s):  
Research Work ◽  
Optimum Temperature ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Plate Method ◽  
Sterile Culture ◽  
Escherchia Coli ◽  
Optimum Ph ◽  
L1 And L2

In recent research work bacteria were isolated from samples of leucorrhea patients admitted in Lady Wellington hospital Lahore (gynae ward) and Basheer welfare hospital Shahdara Lahore. The sampling was done from pregnant and non-pregnant leucorrhea patients aged 18 to 30 years by using sterile culture sticks from vagina. The samples were spread on agar plates and incubated for overnight, bacterial strains were isolated by streak plate method. The strains were named L1, L2, L3 and L4. Identification was carried out by various morphological and biochemical tests. Molecular characterization was also done to characterize bacteria up to species level. L1 strain was identified as Streptococcus pyogens, L2 as Staphylococcus aureus, L3 as Neisseria gonorrheae and L4 as Escherchia coli. Antibiotic resistance was analyzed by disc plate method.  L1, L2 and L3 strains showed maximum sensitivity with Cefepime antibiotics having values 17.74 µg/ml, 13.63 µg/ml and 12 µg/ml respectively. L4 showed max sensitivity with Azithromycin and Cloxacillin antibiotics i.e., 6.25 µg/ml and 6.4 µg/ml respectively. Optimum pH was 6.5 for L1 and L2, while 7 for L3 and L4. Optimum temperature was 37 for all strains.

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